绿萝花及其多糖对S180荷瘤小鼠免疫细胞的影响
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摘要
以水萃取和醇沉法制备的绿萝花浸膏和粗多糖为研究材料,以流式细胞术为检测手段,S180荷瘤小鼠CTL、HTL、NK细胞为检测指标,探讨绿萝花对荷瘤小鼠肿瘤免疫功能的影响。结果表明,小鼠接种S180瘤细胞后,血液中CD3~+T细胞数量下降,口服绿萝花浸膏、粗多糖口服液和环磷酰胺后,在不同检测时间点,荷瘤小鼠血液中CD3~+T细胞数量升高,且随着给药时间的延长,效果越明显,说明绿萝花(1g/kg～2.0g/kg)及绿萝花多糖(100mg/kg)连续口服7d～28d,具有升高由S180瘤细胞引起的小鼠血液中CD3~+T细胞数量下降趋势的作用;绿萝花(1.0g/kg)及绿萝花多糖(100mg/kg)连续口服14d～28d,具有升高S180瘤荷小鼠和健康小鼠CD335~+T细胞数量的作用,为绿萝花进一步开发利用提供试验依据。
In this research,Scindapsus aureus extractum and crude polysaccharides were prepared by aqueous extraction method and alcohol precipitation method as research material,and flow cytometry was used as the detection method,CTL-cells,HTL-cells and NK-cells of S180 mice were detected.The result showed that the number of CD3~+T cells in the blood decreased after inoculation with S180 tumor cells.After oral administration of Scindapsus aureus extractum,crude polysaccharide oral liquid and cyclophosphamide,CD3~+T cells in blood of tumor-bearing mice increased at different detection time points,and with the prolongation of medication,the effect was more obvious.Scindapsus aureus(1 g/kg-2.0 g/kg)and polysaccharides(100 mg/kg)were continuously administrated for 7 d-28 d,the numbers of CD3~+T cells in blood of mice which had descending trend caused by S180-tumor cells increased.Scindapsus aureus(1 g/kg)and polysaccharides(100 mg/kg)were continuously administrated for 14 d-28 d,the numbers of CD335~+T cells in S180-bearing mice and healthy mice increased.The results provided experimental basis for further development and utilization of Scindapsus aureus.
In this research,Scindapsus aureus extractum and crude polysaccharides were prepared by aqueous extraction method and alcohol precipitation method as research material,and flow cytometry was used as the detection method,CTL-cells,HTL-cells and NK-cells of S180 mice were detected.The result showed that the number of CD3~+T cells in the blood decreased after inoculation with S180 tumor cells.After oral administration of Scindapsus aureus extractum,crude polysaccharide oral liquid and cyclophosphamide,CD3~+T cells in blood of tumor-bearing mice increased at different detection time points,and with the prolongation of medication,the effect was more obvious.Scindapsus aureus(1 g/kg-2.0 g/kg)and polysaccharides(100 mg/kg)were continuously administrated for 7 d-28 d,the numbers of CD3~+T cells in blood of mice which had descending trend caused by S180-tumor cells increased.Scindapsus aureus(1 g/kg)and polysaccharides(100 mg/kg)were continuously administrated for 14 d-28 d,the numbers of CD335~+T cells in S180-bearing mice and healthy mice increased.The results provided experimental basis for further development and utilization of Scindapsus aureus.
引文
[1]王赛,刘群,韩金潭,等.藏药绿萝花对高血糖小鼠模型的治疗试验[J].动物医学进展,2015,36(4):67-72.
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[9]韩金潭,孙翠翠,丁淑梅,等.藏药绿萝花水提物对成纤维细胞线粒体抗氧化作用的研究[J].动物医学进展,2017,38(2):55-59.
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[13]孙翠翠,程江,唐棣,等.藏药绿萝花水溶性多糖提取工艺研究[J].黑龙江畜牧兽医,2017,4(上):208-213.
[14]张珏,谭雨龙,杨菲,等.肿瘤环境影响抗感染CD8+T细胞应答启动的初步研究[J].免疫学杂志,2013,29(3):229-233.
[15]胡春萍,胡婷婷,蔡雪婷,等.桂枝茯苓胶囊对子宫内膜异位症大鼠脾脏CD4+T淋巴细胞数和NK细胞杀伤活性的影响[J].中国实验方剂学杂志,2011,17(9):145-148.
[16]严健,原永明,张舒,等.CD3+、CD4+、CD8+T淋巴细胞亚群在肿瘤患者外周血中检测的临床意义[J].检验医学,2013,28(10):901-903.
[17]王锦国.小鼠胃癌模型中T淋巴细胞与NK细胞的改变[D].新疆石河子:石河子大学,2014.
[2]罗小文,顾健,张娥,等.藏药绿萝花不同活性部位降血糖实验研究[J].中药材,2012,35(4):612-614.
[3]韩金潭,刘群,王赛,等.藏药绿萝花对正常小鼠糖耐量影响的试验研究[J].西南民族大学学报:自然科学版,2014,40(4):518-522.
[4]张晓英,张致英,王鹏翔,等.绿萝花提取物对α-葡萄糖苷酶的抑制及降血糖作用[J].中药药理与临床,2014,30(3):107-111.
[5]王赛,刘群,韩金潭,等.绿萝花对高血脂大鼠的影响[J].中药药理与临床,2014,30(4):84-87.
[6]张晓英,张致英,贺学,等.西藏绿萝花石油醚提取物对STZ糖尿病大鼠糖脂代谢及糖尿病肾病的影响[J].中药药理与临床,2015,31(1):129-133.
[7]孙翠翠,康莲莲,王赛,等.藏药绿萝花抗氧化作用实验研究[J].中兽医医药杂志,2015(5):18-22.
[8]韩金潭,刘群,孙翠翠,等.不同剂量绿萝花对组织器官抗氧化能力的影响[J].黑龙江畜牧兽医,2015(11):24-28.
[9]韩金潭,孙翠翠,丁淑梅,等.藏药绿萝花水提物对成纤维细胞线粒体抗氧化作用的研究[J].动物医学进展,2017,38(2):55-59.
[10]杨荣,刘群,康莲莲,等.藏药绿萝花体外抑制肿瘤细胞生长的实验研究[J].中成药,2015,37(9):2061-2065.
[11]孙翠翠,朱子凤,刘群.绿萝花水溶性多糖体外抗肿瘤的实验研究[J].西南民族大学学报:自然科学版,2017,43(3):268-275.
[12]康莲莲,刘群,王赛,等.藏药绿萝花总化学成分水提工艺试验[J].中国兽医杂志,2015,51(3):84-86.
[13]孙翠翠,程江,唐棣,等.藏药绿萝花水溶性多糖提取工艺研究[J].黑龙江畜牧兽医,2017,4(上):208-213.
[14]张珏,谭雨龙,杨菲,等.肿瘤环境影响抗感染CD8+T细胞应答启动的初步研究[J].免疫学杂志,2013,29(3):229-233.
[15]胡春萍,胡婷婷,蔡雪婷,等.桂枝茯苓胶囊对子宫内膜异位症大鼠脾脏CD4+T淋巴细胞数和NK细胞杀伤活性的影响[J].中国实验方剂学杂志,2011,17(9):145-148.
[16]严健,原永明,张舒,等.CD3+、CD4+、CD8+T淋巴细胞亚群在肿瘤患者外周血中检测的临床意义[J].检验医学,2013,28(10):901-903.
[17]王锦国.小鼠胃癌模型中T淋巴细胞与NK细胞的改变[D].新疆石河子:石河子大学,2014.