隐匿性乙肝患者转化生长因子、干扰素及部分细胞因子表达情况分析
|
摘要
目的:探讨转化生长因子-β1(TGF-β1)、干扰素-γ(IFN-γ)和人可溶程序性死亡分子1(sPD-1)、可溶性B7-H2(sB7-H2)、可溶性B7-H3(sB7-H3)细胞因子在隐匿性乙肝病毒感染(OBI)形成过程中的作用。方法:选取OBI标本20例、乙肝患者标本16例、乙肝携带者标本16例及健康献血者标本16例,同时检测血清中TGF-β1、IFN-γ、sPD-1、sB7-H2、sB7-H3,对检测结果进行统计分析。结果:sB7-H2、sPD-1和IFN-γ在不同人群血清中的含量差异无统计学意义(P>0.05),而sB7-H3、TGF-β1在不同人群血清中的含量差异有统计学意义(P<0.05),OBI人群血清中sB7-H3含量与乙肝患者及乙肝携带者比较均差异无统计学意义(P>0.05),与健康献血者比较有所增高(P<0.05),OBI人群血清中TGF-β1含量与乙肝患者人群及乙肝携带者比较差异无统计学意义(P>0.05),与健康献血者比较有所降低(P<0.05)。结论:sB7-H3和TGF-β1在OBI发生和发展中起着比较重要的作用,由于sB7-H3和TGF-β1均为免疫调节性细胞因子,因此免疫因素是OBI形成过程中的重要影响因素。
Objective:To evaluate the role of the TGF-β1,IFN-γ,sPD-1,sB7-H2 and sB7-H3 in the formation of OBI.Method:20 specimens of OBI,16 specimens of hepatitis B patients,16 specimens of hepatitis B carriers and16 specimens of healthy blood donors were selected,the TGF-β1,IFN-γ,sPD-1,sB7-H2 and sB7-H3 were tested,the result were statistical analyzed.Result:There was no significant difference in sB7-H2,sPD-1 and IFN-γlevel in different groups(P>0.05).sB7-H3 and TGF-β1 were statistically different in different groups(P<0.05),There were no significant difference in sB7-H3 level between OBI group and HBV patients,HBV carriers(P>0.05),Compared to healthy blood donors,The sB7-H3 was higher in OBI group(P<0.05).There were no significant difference in TGF-β1 level between OBI group and HBV patients,HBV carriers(P>0.05).Compared to healthy blood donors,The TGF-β1 was lower in OBI group(P<0.05).Conclusion:sB7-H3 and TGF-β1 play an important role in the occurrence and development of OBI,sB7-H3 and TGF-β1 are immune regulatory cytokine,so immune factors is important factor in the formation of OBI.
Objective:To evaluate the role of the TGF-β1,IFN-γ,sPD-1,sB7-H2 and sB7-H3 in the formation of OBI.Method:20 specimens of OBI,16 specimens of hepatitis B patients,16 specimens of hepatitis B carriers and16 specimens of healthy blood donors were selected,the TGF-β1,IFN-γ,sPD-1,sB7-H2 and sB7-H3 were tested,the result were statistical analyzed.Result:There was no significant difference in sB7-H2,sPD-1 and IFN-γlevel in different groups(P>0.05).sB7-H3 and TGF-β1 were statistically different in different groups(P<0.05),There were no significant difference in sB7-H3 level between OBI group and HBV patients,HBV carriers(P>0.05),Compared to healthy blood donors,The sB7-H3 was higher in OBI group(P<0.05).There were no significant difference in TGF-β1 level between OBI group and HBV patients,HBV carriers(P>0.05).Compared to healthy blood donors,The TGF-β1 was lower in OBI group(P<0.05).Conclusion:sB7-H3 and TGF-β1 play an important role in the occurrence and development of OBI,sB7-H3 and TGF-β1 are immune regulatory cytokine,so immune factors is important factor in the formation of OBI.
引文
[1]Wang S,Tao Y,Tao Y,et al.Epidemiological study of hepatitis B and hepatitis C infections in Northeastern China and the beneficial effect of the vaccination strategy for hepatitis B:a cross-sectional study[J].BMCPublic Health,2018,18:1088-1088.
[2]徐国美,王鹏,李美霖,等.北京部分地区献血人群血液检测结果分析[J].临床血液学杂志,2016,29(10):833-835.
[3]周怡,蒋靓,王凯,等.HBsAg阴性HBV DNA阳性无偿献血者的血清学模式及其相关特征研究[J].临床血液学杂志,2017,30(2):86-89.
[4]石庆秋,苏武锦,何平,等.南宁市机采血小板献血者血液核酸筛查情况分析[J].临床血液学杂志,2017,30(4):320-322.
[5]朱守兵,刘祎,傅立强.绍兴地区无偿献血者血液核酸检测效果评估[J].临床血液学杂志,2016,29(10):831-832.
[6]Levicnik-Stezinar S,Rahne-Potokar U,Candotti D,et al.Anti-HBs positive occult hepatitis B virus carrier blood infectious in two transfusion recipients[J].JHepatol,2008,48:1022-1025.
[7]Borzooy Z,Jazayeri SM,Mirshafiey A,et al.Identification of occult hepatitis B virus(HBV)infection and viral antigens in healthcare workers who presented low to moderate levels of anti-HBs after HBV vaccination[J].Germs,2015,5:134-140.
[8]Rastegarvand N,Makvandi M,Samarbafzadeh A,et al.Molecular Characterization of Pre-Core/Core and SRegion of Hepatitis B Virus in Hemodialysis Patients With Occult Hepatitis B Infection[J].Jundishapur JMicrobiol,2015,8:1-5.
[9]Kramvis A.Molecular characteristics and clinical relevance of African genotypes and subgenotypes of hepatitis B virus[J].J Med Viral,2018,108:17-21.
[10]Hatazawa Y,Yano Y,Okada R,et al.Quasispecies variant of pre-S/S gene in HBV-related hepatocellular carcinoma with HBs antigen positive and occult infection[J].Infect Agent Cancer,2018,13:1-8.
[11]Diarra B,Yonli AT,Sorgho PA,et al.Occult Hepatitis B Virus Infection and Associated Genotypes among HBsAg-negative Subjects in Burkina Faso[J].Mediterr J Hematol Infect Dis,2018,10:2018007.
[12]李锦,景媛媛,郭燕,等.隐匿性乙肝献血者中TGF-β1的表达分析[J].中国输血杂志,2015,29(1):39-41.
[13]Zeng Z,Li L,Chen Y,et al.Interferon-γfacilitates hepatic antiviral T cell retention for the maintenance of liver-induced systemic tolerance[J].J Exp Med,2016,213:1079-1093.
[14]李曼,孙学华,周振华,等.慢性乙型肝炎病毒感染者外周血T细胞γ干扰素和白细胞介素4的水平变化[J].细胞与分子免疫学杂志,2016,32(2):240-244.
[15]操伟庆,季榕.慢性乙型肝炎患者血清中IL-17A和TGF-β1水平的变化及意义[J].贵州医科大学学报,2017,42(12):1431-1435.
[16]任彦,袁丹,张威威,等.干扰素对于病毒性肝炎治疗效果的观察[J].临床医药文献电子杂志,2018,4(46):69-70.
[17]G Liang,G Liu,K Kitamura,et al.TGF-βsuppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex[J].Plos Pathogens,2015,11:18-23.
[18]Huang J,Wu Z,Lu S,et al.Soluble B7-H2as a novel marker in early evaluation of the severity of acute pancreatitis[J].Lab Med,2015,46:109-117.
[19]Xu H,Chen X,Tao M.B7-H3and B7-H4are independent predictors of a poor prognosis in patients with pancreatic cancer[J].Oncol Lett,2016,11:1841-1846.
[20]Staszewski J,Piusińska-Macoch R,Brodacki B,et al.IL-6,PF-4,sCD40L,and homocysteine are associated with the radiological progression of cerebral smallvessel disease:a 2-year follow-up study[J].Clin Interv Aging,2018,13:1135-1141.
[21]金静霞,郑翠苹,陈丽雅,等.PD-1、PD-L1在弥漫大B细胞淋巴瘤组织中的差异性表达及其临床意义[J].临床血液学杂志,2018,31(1):34-37.
[22]Zhang ZH,Zhang L,Dai Y,et al.Occult hepatitis Bvirus infection:influence of S protein variants[J].Virol J,2016,13:1186-1197.
[23]Sadeghi A,Yahyapour Y,Poortahmasebi V.Clearance of HBV DNA in immunized children born to HBsAgpositive mothers,years after being diagnosed with occult HBV infection[J].J Viral Hepat,2016,23:282-285.
[24]Gill H,Leung GMK,Seto WK,et al.Risk of viral reactivation in patients with occult hepatitis B virus infection during ruxolitinib treatment[J].Ann Hematol,2018,10:1-4.
[25]黄坚,张光波,何广胜,等.可溶性B7-H2在早期急性胰腺炎严重程度判定中的应用及其临床意义[J].中国实用内科杂志,2015,35(3):236-238.
[26]时翠林,徐俊驰,宋华峰,等.HBV感染患者外周血中sB7-H3的表达及临床意义[J].江苏医药,2017,43(6):408-410.
[27]李成德,全毅,蔡健梅,等.HBV相关原发性肝癌患者血清B7-H3与IL-21的表达及其临床意义[J].中国免疫学杂志,2018,34(4):569-575.
[28]Stanilova S,Stanilov N,Julianov A,et al.Transforming growth factor-β1gene promoter-509C/T polymorphism in association with expression affects colorectal cancer development and depends on gender[J].PLoS One,2018,13:e0201775.
[2]徐国美,王鹏,李美霖,等.北京部分地区献血人群血液检测结果分析[J].临床血液学杂志,2016,29(10):833-835.
[3]周怡,蒋靓,王凯,等.HBsAg阴性HBV DNA阳性无偿献血者的血清学模式及其相关特征研究[J].临床血液学杂志,2017,30(2):86-89.
[4]石庆秋,苏武锦,何平,等.南宁市机采血小板献血者血液核酸筛查情况分析[J].临床血液学杂志,2017,30(4):320-322.
[5]朱守兵,刘祎,傅立强.绍兴地区无偿献血者血液核酸检测效果评估[J].临床血液学杂志,2016,29(10):831-832.
[6]Levicnik-Stezinar S,Rahne-Potokar U,Candotti D,et al.Anti-HBs positive occult hepatitis B virus carrier blood infectious in two transfusion recipients[J].JHepatol,2008,48:1022-1025.
[7]Borzooy Z,Jazayeri SM,Mirshafiey A,et al.Identification of occult hepatitis B virus(HBV)infection and viral antigens in healthcare workers who presented low to moderate levels of anti-HBs after HBV vaccination[J].Germs,2015,5:134-140.
[8]Rastegarvand N,Makvandi M,Samarbafzadeh A,et al.Molecular Characterization of Pre-Core/Core and SRegion of Hepatitis B Virus in Hemodialysis Patients With Occult Hepatitis B Infection[J].Jundishapur JMicrobiol,2015,8:1-5.
[9]Kramvis A.Molecular characteristics and clinical relevance of African genotypes and subgenotypes of hepatitis B virus[J].J Med Viral,2018,108:17-21.
[10]Hatazawa Y,Yano Y,Okada R,et al.Quasispecies variant of pre-S/S gene in HBV-related hepatocellular carcinoma with HBs antigen positive and occult infection[J].Infect Agent Cancer,2018,13:1-8.
[11]Diarra B,Yonli AT,Sorgho PA,et al.Occult Hepatitis B Virus Infection and Associated Genotypes among HBsAg-negative Subjects in Burkina Faso[J].Mediterr J Hematol Infect Dis,2018,10:2018007.
[12]李锦,景媛媛,郭燕,等.隐匿性乙肝献血者中TGF-β1的表达分析[J].中国输血杂志,2015,29(1):39-41.
[13]Zeng Z,Li L,Chen Y,et al.Interferon-γfacilitates hepatic antiviral T cell retention for the maintenance of liver-induced systemic tolerance[J].J Exp Med,2016,213:1079-1093.
[14]李曼,孙学华,周振华,等.慢性乙型肝炎病毒感染者外周血T细胞γ干扰素和白细胞介素4的水平变化[J].细胞与分子免疫学杂志,2016,32(2):240-244.
[15]操伟庆,季榕.慢性乙型肝炎患者血清中IL-17A和TGF-β1水平的变化及意义[J].贵州医科大学学报,2017,42(12):1431-1435.
[16]任彦,袁丹,张威威,等.干扰素对于病毒性肝炎治疗效果的观察[J].临床医药文献电子杂志,2018,4(46):69-70.
[17]G Liang,G Liu,K Kitamura,et al.TGF-βsuppression of HBV RNA through AID-dependent recruitment of an RNA exosome complex[J].Plos Pathogens,2015,11:18-23.
[18]Huang J,Wu Z,Lu S,et al.Soluble B7-H2as a novel marker in early evaluation of the severity of acute pancreatitis[J].Lab Med,2015,46:109-117.
[19]Xu H,Chen X,Tao M.B7-H3and B7-H4are independent predictors of a poor prognosis in patients with pancreatic cancer[J].Oncol Lett,2016,11:1841-1846.
[20]Staszewski J,Piusińska-Macoch R,Brodacki B,et al.IL-6,PF-4,sCD40L,and homocysteine are associated with the radiological progression of cerebral smallvessel disease:a 2-year follow-up study[J].Clin Interv Aging,2018,13:1135-1141.
[21]金静霞,郑翠苹,陈丽雅,等.PD-1、PD-L1在弥漫大B细胞淋巴瘤组织中的差异性表达及其临床意义[J].临床血液学杂志,2018,31(1):34-37.
[22]Zhang ZH,Zhang L,Dai Y,et al.Occult hepatitis Bvirus infection:influence of S protein variants[J].Virol J,2016,13:1186-1197.
[23]Sadeghi A,Yahyapour Y,Poortahmasebi V.Clearance of HBV DNA in immunized children born to HBsAgpositive mothers,years after being diagnosed with occult HBV infection[J].J Viral Hepat,2016,23:282-285.
[24]Gill H,Leung GMK,Seto WK,et al.Risk of viral reactivation in patients with occult hepatitis B virus infection during ruxolitinib treatment[J].Ann Hematol,2018,10:1-4.
[25]黄坚,张光波,何广胜,等.可溶性B7-H2在早期急性胰腺炎严重程度判定中的应用及其临床意义[J].中国实用内科杂志,2015,35(3):236-238.
[26]时翠林,徐俊驰,宋华峰,等.HBV感染患者外周血中sB7-H3的表达及临床意义[J].江苏医药,2017,43(6):408-410.
[27]李成德,全毅,蔡健梅,等.HBV相关原发性肝癌患者血清B7-H3与IL-21的表达及其临床意义[J].中国免疫学杂志,2018,34(4):569-575.
[28]Stanilova S,Stanilov N,Julianov A,et al.Transforming growth factor-β1gene promoter-509C/T polymorphism in association with expression affects colorectal cancer development and depends on gender[J].PLoS One,2018,13:e0201775.