Novozym435催化月桂酸单甘酯的合成研究
|
摘要
本文研究了在无溶剂条件下Novozym435脂肪酶催化合成月桂酸单甘酯,采用高碘酸氧化法和酸值滴定法对产品进行单甘酯含量及月桂酸含量的分析。同时研究了温度、月桂酸与甘油的摩尔比、酶用量、反应时间、水用量、分子筛用量等六个因素对反应的影响,并选用L25(65)正交试验,探讨合成月桂酸单甘酯的最佳条件。结果表明:56℃下,月桂酸与甘油摩尔比为1∶1.5,酶用量为0.8%,反应15 h,水用量为0.56%,分子筛用量为2%,月桂酸单甘酯含量达到81.5%。
In this paper, the synthesis of glycerol monolaurate catalyzed by Novozym435 lipase in the absence of solvent was studied. The monoglyceride content and lauric acid content were analyzed by oxidation of periodate acid and titration of acid. The effects of temperature, molar ratio of lauric acid and glycerol, enzyme dosage, reaction time, water dosage and molecular sieve dosage in the reaction were also studied. To identify the best conditions for the synthesis of monoglyceride lauric acid were studied by using the orthogonal test of L25(65). The results showed that: at 56 ℃, the molar ratio(acid:glycerol) was 1∶1.5, the amount of enzyme was 0.8 %, the reaction time was 15 h, the amount of water was 0.56 %, the amount of molecular sieve was 2 %, and the content of glycerol mono laurate reached 81.5 %.
In this paper, the synthesis of glycerol monolaurate catalyzed by Novozym435 lipase in the absence of solvent was studied. The monoglyceride content and lauric acid content were analyzed by oxidation of periodate acid and titration of acid. The effects of temperature, molar ratio of lauric acid and glycerol, enzyme dosage, reaction time, water dosage and molecular sieve dosage in the reaction were also studied. To identify the best conditions for the synthesis of monoglyceride lauric acid were studied by using the orthogonal test of L25(65). The results showed that: at 56 ℃, the molar ratio(acid:glycerol) was 1∶1.5, the amount of enzyme was 0.8 %, the reaction time was 15 h, the amount of water was 0.56 %, the amount of molecular sieve was 2 %, and the content of glycerol mono laurate reached 81.5 %.
引文
[1]肖付刚,罗松明,刘钟栋.多元醇脂肪酸酯的防腐保鲜效果[J].中国食品添加剂,2006,1:87-92.
[2]游利琴,肖咏梅,张风雷.月桂酸单甘酯的催化合成[J].广东化工,2011,38(9):273-274.
[3]宋国胜,郭祀远.离子交换树脂催化月桂酸单甘油酯的合成[J].中国油脂,2001,26(5):89-90.
[4]朱广琪,李佳敏,张晓芳,等.Ce改性硅钨酸催化制备月桂酸单甘酯[J].精细化工,2018.
[5]崔海萍.脂肪酶催化合成月桂酸单甘酯和乙酸癸酯[D].大连理工大学,2012.
[6]吕德水,石明孝.单脂肪酸甘油酯总单酯含量的测定[J].日用化学工业,1996,3:39-40.
[7]Couturier L,Taupin D,Yvergnaux F.Lipase-catalyzed chemoselective aminolysis of various aminoalcohols with fatty acids[J].Journal of molecular catalysis B:Enzymatic,2009,56(1):29-33.
[8]Nordblad M.,Adlercreutz P.Efficient enzymatic acrylation through transesterification at controlled water activity[J].Biotechnology and bioengineering,2010,99(6):1518-1524.
[2]游利琴,肖咏梅,张风雷.月桂酸单甘酯的催化合成[J].广东化工,2011,38(9):273-274.
[3]宋国胜,郭祀远.离子交换树脂催化月桂酸单甘油酯的合成[J].中国油脂,2001,26(5):89-90.
[4]朱广琪,李佳敏,张晓芳,等.Ce改性硅钨酸催化制备月桂酸单甘酯[J].精细化工,2018.
[5]崔海萍.脂肪酶催化合成月桂酸单甘酯和乙酸癸酯[D].大连理工大学,2012.
[6]吕德水,石明孝.单脂肪酸甘油酯总单酯含量的测定[J].日用化学工业,1996,3:39-40.
[7]Couturier L,Taupin D,Yvergnaux F.Lipase-catalyzed chemoselective aminolysis of various aminoalcohols with fatty acids[J].Journal of molecular catalysis B:Enzymatic,2009,56(1):29-33.
[8]Nordblad M.,Adlercreutz P.Efficient enzymatic acrylation through transesterification at controlled water activity[J].Biotechnology and bioengineering,2010,99(6):1518-1524.