输合配穴针刺对脑损伤幼鼠少突胶质细胞Nogo-A、MAG、OMgp表达的影响
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摘要
目的:探讨输合配穴针刺干预对LPS脑损伤幼鼠模型脑组织Nogo-A、MAG、OMgp表达的影响。方法:将SPF级SD新生的乳鼠60只随机分为LPS组乳鼠干预组,LPS组乳鼠不干预组和对照组乳鼠,对干预组乳鼠采用输合配穴针刺21 d,针刺后对各组大鼠脑组织匀浆中Nogo-A、MAG、OMgp含量进行检测。结果:脑组织Nogo-A、MAG、OMgp的表达与脑损伤程度密切相关。模型组MAG明显低于空白对照组(P<0.01),Nogo-A、OMgp的表达高于空白对照组(P<0.01)。并且在7、14、21 d时,结果显示:脑组织MAG表达在针刺干预组表达高于未干预组,Nogo-A、OMgp表达在针刺干预组表达低于未干预组。结论:通过对LPS仔鼠感染脑损伤检测,脑组织Nogo-A、MAG、OMgp表达,输合配穴针刺可以促进MAG对脑神经的生长作用,同时抑制Nogo-A,OMgp对脑神经再生的抑制作用,促进神经发育,保护神经。
Objective: To explore the expressions of Nogo-A/MAG and OMgp in brain damage of LPS premature rats which were treated by acupuncture at the Shu and He acupoints. Method: Sixty SPF SD neonatal rats were randomly divided into LPS intervention group,LPS non-interventional group and blank control group. Interventional group adopted acupuncture and manipulation at the Shu and He acupoints within 21 days. Expressions of Nogo-A/MAG and OMgp in brain tissue would be tested after treatment. Result: Expressions of Nogo-A/MAG and OMgp in brain tissue were relevant to brain damage degree. The content of Nogo-A/MAG in non-interventional group was significantly lower than that in the control group( P < 0. 01),while the content of OMgp in interventional group was obviously higher than that in the control group( P < 0. 01). The content of Nogo-A/MAG in nterventional group was higher than that in the non-interventional group at 7 th,14 th and 21 th day. The content of OMgp in nterventional group was lower than that in non-interventional group at 7 th,14 th and 21 th day. Conclusion: Through the examintion of Nogo-A/MAG and OMgp in brain damage of LPS neonatal rats,acupuncture at the Shu and He acupoints could help nerve generation,inhibit Nogo-A and OMgp to promot normal development.
Objective: To explore the expressions of Nogo-A/MAG and OMgp in brain damage of LPS premature rats which were treated by acupuncture at the Shu and He acupoints. Method: Sixty SPF SD neonatal rats were randomly divided into LPS intervention group,LPS non-interventional group and blank control group. Interventional group adopted acupuncture and manipulation at the Shu and He acupoints within 21 days. Expressions of Nogo-A/MAG and OMgp in brain tissue would be tested after treatment. Result: Expressions of Nogo-A/MAG and OMgp in brain tissue were relevant to brain damage degree. The content of Nogo-A/MAG in non-interventional group was significantly lower than that in the control group( P < 0. 01),while the content of OMgp in interventional group was obviously higher than that in the control group( P < 0. 01). The content of Nogo-A/MAG in nterventional group was higher than that in the non-interventional group at 7 th,14 th and 21 th day. The content of OMgp in nterventional group was lower than that in non-interventional group at 7 th,14 th and 21 th day. Conclusion: Through the examintion of Nogo-A/MAG and OMgp in brain damage of LPS neonatal rats,acupuncture at the Shu and He acupoints could help nerve generation,inhibit Nogo-A and OMgp to promot normal development.
引文
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[14]朱庄臣,倪斌.以Nogo蛋白及其受体为靶点促进神经再生的研究进展[J].第二军医大学学报,2010,31(6):670-673.
[15]张高迎,张阳,徐华,等.针灸督脉经穴对脑缺血神经再生抑制因子MAG和OMgp蛋白表达的影响[J].中医药杂志,2016,28(8):1100-1104.
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[19]张洁,陈采玲.针刺治疗痉挛型脑瘫小儿运动功能障碍临床研究进展[J].光明中医,2017,32(2):303-307.
[20]马允,杨帆,昂文平.针刺对脑损伤保护机制的实验研究进展[J].广州中医药大学学报,2013,30(1):120-128.
[21]刘启华,陈永斌,何泉,等.基于自噬理论探讨补气活血方促进缺血性脑损伤血管新生[J].辽宁中医杂志,2017,44(7):1410-1412.
[22]吴涛,陈靖轩,安鹏飞,等.电针对颅脑损伤大鼠脑组织中AQP-4表达的影响[J].辽宁中医杂志,2017,44(6):1311-1315.
[23]顾玉宝,刘敬霞,王枫,等.针刺联合中药治疗缺血性脑卒中临床应用研究进展[J].辽宁中医药大学学报,2017,19(7):87-90.
[24]宋春侠,暴宏伶,吴松柏,等.泻土化瘀方配合针刺治疗顽固性失眠的临床疗效评价[J].辽宁中医杂志,2017,44(9):1873-1875.
[2]李楠,王雪峰,张亮,等.输合配穴针刺法促进LPS早产鼠脑损伤中CNP及MBP表达的研究[J].山东医药,2012,52(36):55-57.
[3]谭雄.高危新生儿早期干预的临床疗效分析[J].中国医药指南,2012,10(8):534-535.
[4]李芩,黄栋,叶莉,等.康复训练联合针灸治疗小儿脑瘫运动功能障碍的效果分析[J].中华中医药学刊,2016,34(8):1994-1996.
[5]王雪峰.中西医结合儿科学[M].北京:中国中医药出版社,2005:216.
[6]李冬梅,沈红岩.输合配穴针刺法在痉挛型脑瘫患儿治疗中的应用[J].中国中医药现代远程教育,2011,9(8):47-49.
[7]胡晓丽,王雪峰,庞伟,等.输合配穴针刺干预对不同脑损伤模型比较[J].华南国防医学杂志,2015,29(4):251-254.
[8]胡晓丽,王雪峰,庞伟,等.输合配穴针刺对LPS脑损伤幼鼠模型脑组织S100B、NSE表达的影响[J].中国中西医结合儿科学,2015,7(1):20-22.
[9]邓春雷.实验针灸学[M].北京:人民卫生出版社,1998:7.
[10]陈宜张.分子神经生物学[M].北京:人民军医出版社,1995:247-266.
[11]张超,潘亚文.脊髓损伤后神经再生抑制因子的研究进展[J].国际神经病学神经外科学杂志,2014,32(4):561-564.
[12]马睿杰,聂小峰,肖婷,等.少突胶质细胞相关因子与脊髓损伤的研究进展[J].中华中医药学刊,2001,16(3):720-722.
[13]卢艳.少突胶质细胞在中枢神经发育与再生中的作用机制研究[D].上海:第二军医大学,2013.
[14]朱庄臣,倪斌.以Nogo蛋白及其受体为靶点促进神经再生的研究进展[J].第二军医大学学报,2010,31(6):670-673.
[15]张高迎,张阳,徐华,等.针灸督脉经穴对脑缺血神经再生抑制因子MAG和OMgp蛋白表达的影响[J].中医药杂志,2016,28(8):1100-1104.
[16]郭江波,金冬梅.新生大鼠缺氧缺血性脑损伤后脑组织中OMgp的表达[J].中国新生儿科杂志,2015,30(6):454-457.
[17]王雪峰,胡晓丽.中医对小儿痉挛型脑瘫(肝强脾弱证)的探析[J].中医儿科杂志,2005(2):6-7.
[18]张静.输合配穴法针刺对痉挛型脑瘫患儿下肢痉挛状态改善的临床研究[J].针灸临床杂志,2016,32(3):21-23.
[19]张洁,陈采玲.针刺治疗痉挛型脑瘫小儿运动功能障碍临床研究进展[J].光明中医,2017,32(2):303-307.
[20]马允,杨帆,昂文平.针刺对脑损伤保护机制的实验研究进展[J].广州中医药大学学报,2013,30(1):120-128.
[21]刘启华,陈永斌,何泉,等.基于自噬理论探讨补气活血方促进缺血性脑损伤血管新生[J].辽宁中医杂志,2017,44(7):1410-1412.
[22]吴涛,陈靖轩,安鹏飞,等.电针对颅脑损伤大鼠脑组织中AQP-4表达的影响[J].辽宁中医杂志,2017,44(6):1311-1315.
[23]顾玉宝,刘敬霞,王枫,等.针刺联合中药治疗缺血性脑卒中临床应用研究进展[J].辽宁中医药大学学报,2017,19(7):87-90.
[24]宋春侠,暴宏伶,吴松柏,等.泻土化瘀方配合针刺治疗顽固性失眠的临床疗效评价[J].辽宁中医杂志,2017,44(9):1873-1875.