我国牛羊流行性出血病血清抗体调查
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摘要
为了解流行性出血病(EHD)在我国的流行和分布情况,本研究采用竞争ELISA试验方法,对2015年～2017年我国吉林、河北、西藏、重庆、云南等15个省区的6 413份牛羊血清样品进行检测。结果显示,未发现绵羊EHDV阳性血清,山羊EHDV血清阳性率较低(0～4%),而牛血清EHDV阳性率有明显的地域性和季节性,由北向南逐渐升高(0～65.83%),表明EHD在我国的流行已具有广泛性;秋季阳性率(61.11%～65%)明显高于春季(10%～16.67%)和夏季(29%～32%),表明该病的流行分布状况与其媒介昆虫的活动有密切关系。本研究是我国首次大规模的EHD血清学调查,对有效防控该病提供了重要的实验数据。
To investigate the epidemic distribution of epizootic haemorrhagic disease(EHD) in China, a total of 6,413 bovine,goat and sheep sera collected from 15 provinces between 2015 and 2017 were detected for the seroprevalence of the EHD virus(EHDV) by C-ELISA test. The results showed that the positive of sheep sera was not found and very low seropositive rate of goat(0-4%), the positive rate of bovine sera for the EHDV increases from north to south(from 0 to 65.83%). In addition, the results showed that the infection rate in autumn(September to November) was the highest(61.11% to 65%), which was significantly higher than that in spring(10% to 16.67%) and summer(29% to 32%). On the one hand our findings showed that EHD was prevalent and provide the first reliable reference regarding EHD surveillance in China, on the other hand it was verified that the prevalence of EHD was closely related to the activities of its media insects and has obvious regional and seasonal characteristics.
To investigate the epidemic distribution of epizootic haemorrhagic disease(EHD) in China, a total of 6,413 bovine,goat and sheep sera collected from 15 provinces between 2015 and 2017 were detected for the seroprevalence of the EHD virus(EHDV) by C-ELISA test. The results showed that the positive of sheep sera was not found and very low seropositive rate of goat(0-4%), the positive rate of bovine sera for the EHDV increases from north to south(from 0 to 65.83%). In addition, the results showed that the infection rate in autumn(September to November) was the highest(61.11% to 65%), which was significantly higher than that in spring(10% to 16.67%) and summer(29% to 32%). On the one hand our findings showed that EHD was prevalent and provide the first reliable reference regarding EHD surveillance in China, on the other hand it was verified that the prevalence of EHD was closely related to the activities of its media insects and has obvious regional and seasonal characteristics.
引文
[1]Maclachlan N J, Zientara S, Savini G, et al. Epizootic haemor-rhagic disease[J]. Rev Sci Tech, 2015, 34(2):341-349.
[2]Anbalagan S, Hause B M. Characterization of epizootic hemor-rhagic disease virus from a bovine with clinical disease withhigh nucleotide sequence identity to white-tailed deer isolates[J]. Arch Virol, 2014, 159(10):2737-2740.
[3]Kedmi M, Galon N, Herziger Y. Comparison of the epidemiolo-gy of epizootic haemorrhagic disease and bluetongue viruses indairy cattle in Israel[J]. Vet J, 2011, 190(1):77-83.
[4]Noon T H, Wesche S L, Cagle D. Hemorrhagic disease inbighorn sheep in Arizona[J]. J Wild Dis, 2002, 38(1):172-176.
[5]Maan N S, Maan S, Potgieter A C, et al. Development of real-time RT-PCR assays for detection and typing of epizootic haem-orrhagic disease virus[J]. Transbound Emerg Dis, 2017, 64(6):1120-1132.[6][7][8][9][10][11]
[6]Savini G,Afonso A,Mellor P,et al.Epizootic haemorrhagic disease[J].Res Vet Sci,2011,91(1):1-17.
[7]张胜男,李华春,朱建波,等.2015年内蒙古地区蓝舌病及流行性出血热流行病学调查及血清型鉴定[J].中国预防兽医学报,2016,38(12):939-943.
[8]刘帅,张玲,加帕尔·哈斯木,等.新疆牛群中鹿流行性出血热血清学调查[J].流行病学,2016,33(4):15-18.
[9]和东华,杨振兴,高林,等.云南省边境地区牛流行性出血病病毒血清学调查[J].云南畜牧兽医,2016,(5):7-10.
[10]曹颖颖,吴健敏.广西首例牛源鹿流行性出血热病毒的分离鉴定[J].中国预防兽医学报,2015,37(10):746-750.
[11]吕敏娜,朱建波,李娟,等.广东一株牛源流行性出血病病毒的分离鉴定[J].中国预防兽医学报,2017,39(1):67-70.
[12]Kedmi M,Levi S,Galon N,et al.No evidence for involvement of sheep in the epidemiology of cattle virulent epizootic hemorrhagic disease virus[J].Vet Microbiol,2011,148(24):408-412.
[13]Albayrak H,Ozan E,Gur S.A serologic investigation of epizootic hemorrhagic disease virus(EHDV)in cattle and Gazella subgutturosa subgutturosa in Turkey[J].Trop Anim Health Pro,2010,42(8):1589-1591.
[2]Anbalagan S, Hause B M. Characterization of epizootic hemor-rhagic disease virus from a bovine with clinical disease withhigh nucleotide sequence identity to white-tailed deer isolates[J]. Arch Virol, 2014, 159(10):2737-2740.
[3]Kedmi M, Galon N, Herziger Y. Comparison of the epidemiolo-gy of epizootic haemorrhagic disease and bluetongue viruses indairy cattle in Israel[J]. Vet J, 2011, 190(1):77-83.
[4]Noon T H, Wesche S L, Cagle D. Hemorrhagic disease inbighorn sheep in Arizona[J]. J Wild Dis, 2002, 38(1):172-176.
[5]Maan N S, Maan S, Potgieter A C, et al. Development of real-time RT-PCR assays for detection and typing of epizootic haem-orrhagic disease virus[J]. Transbound Emerg Dis, 2017, 64(6):1120-1132.[6][7][8][9][10][11]
[6]Savini G,Afonso A,Mellor P,et al.Epizootic haemorrhagic disease[J].Res Vet Sci,2011,91(1):1-17.
[7]张胜男,李华春,朱建波,等.2015年内蒙古地区蓝舌病及流行性出血热流行病学调查及血清型鉴定[J].中国预防兽医学报,2016,38(12):939-943.
[8]刘帅,张玲,加帕尔·哈斯木,等.新疆牛群中鹿流行性出血热血清学调查[J].流行病学,2016,33(4):15-18.
[9]和东华,杨振兴,高林,等.云南省边境地区牛流行性出血病病毒血清学调查[J].云南畜牧兽医,2016,(5):7-10.
[10]曹颖颖,吴健敏.广西首例牛源鹿流行性出血热病毒的分离鉴定[J].中国预防兽医学报,2015,37(10):746-750.
[11]吕敏娜,朱建波,李娟,等.广东一株牛源流行性出血病病毒的分离鉴定[J].中国预防兽医学报,2017,39(1):67-70.
[12]Kedmi M,Levi S,Galon N,et al.No evidence for involvement of sheep in the epidemiology of cattle virulent epizootic hemorrhagic disease virus[J].Vet Microbiol,2011,148(24):408-412.
[13]Albayrak H,Ozan E,Gur S.A serologic investigation of epizootic hemorrhagic disease virus(EHDV)in cattle and Gazella subgutturosa subgutturosa in Turkey[J].Trop Anim Health Pro,2010,42(8):1589-1591.