1株耐受Cu~(2+)好氧反硝化菌及其饥饿特性
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摘要
文章通过物料平衡分析阴沟肠杆菌Enterobacter cloacae strain F2 (E. cloacae F2)对不同结合态氮的去除能力;考察铜离子(Cu~(2+))对其反硝化影响并对比驯化前后菌株的性能;探究菌株对低碳氮比(C/N)含Cu~(2+)实际生活污水处理效果及饥饿效应,为工程应用提供借鉴。结果表明,菌株可以有效去除浓度为0～200 mg/L时的硝态氮(NO_3~--N)、亚硝态氮(NO_2~--N)和氨氮(NH_4~+-N);当Cu~(2+)浓度达到80mg/L时菌株对于NO_3~--N的去除效率仍高于80%;驯化前后菌株除氮性能无明显区别,且可以同时去除部分Cu~(2+)。此外,菌株对低C/N污水的处理效果在外加碳源后明显增强;经历饥饿状态后补充碳源,可以刺激细菌并提高脱氮效果。因此,菌株E. cloacae F2具有良好的异养硝化-好氧反硝化能力,对于高C/N含Cu~(2+)污水可以表现良好的处理效果。
Enterobacter cloacae strain F2(E. cloacae F2) was applied for nitrogen removal ability study on different forms of nitrogen by mass balance analysis. Influence of Cu~(2+)on aerobic denitrification and comparison of the strains before/after domestication were investigated. Actual treatment trial and starvation effect of strain E. cloacae F2 were studied with the actual low C/N ratio copper-containing domestic sewage, and the results might be reference for practice. NO_3~--N, NO_2~--N and NH_4~+-N could be degraded effectively by strain E. cloacae F2 within a certain range of concentration(0～200 mg/L). The removal efficiency of NO_3~--N could achieve more than 80% even the concentration of Cu~(2+)was 80 mg/L. The denitrification performances of strains did not show significant differences before or after domestication, and some Cu~(2+)was removed at the same time. The strain was stimulated and nitrogen removal efficiency increased obviously by adding external carbon source.Nitrogen removal ability might also increase by adding external carbon source after starvation. The strain E. cloacae F2 could show satisfied ability of heterotrophic nitrification-aerobic denitrification,efficient treatment ability on high C/N ratio copper-containing domestic sewage.
Enterobacter cloacae strain F2(E. cloacae F2) was applied for nitrogen removal ability study on different forms of nitrogen by mass balance analysis. Influence of Cu~(2+)on aerobic denitrification and comparison of the strains before/after domestication were investigated. Actual treatment trial and starvation effect of strain E. cloacae F2 were studied with the actual low C/N ratio copper-containing domestic sewage, and the results might be reference for practice. NO_3~--N, NO_2~--N and NH_4~+-N could be degraded effectively by strain E. cloacae F2 within a certain range of concentration(0～200 mg/L). The removal efficiency of NO_3~--N could achieve more than 80% even the concentration of Cu~(2+)was 80 mg/L. The denitrification performances of strains did not show significant differences before or after domestication, and some Cu~(2+)was removed at the same time. The strain was stimulated and nitrogen removal efficiency increased obviously by adding external carbon source.Nitrogen removal ability might also increase by adding external carbon source after starvation. The strain E. cloacae F2 could show satisfied ability of heterotrophic nitrification-aerobic denitrification,efficient treatment ability on high C/N ratio copper-containing domestic sewage.
引文
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[21]Guo L J,Zhao B,An Q,et al.Characteristics of a novel aerobic denitrifying bacterium,Enterobacter cloacae strain HNR[J].Applied Biochemistry&Biotechnology,2016,178(5):947-959.
[22]Padhi S K,Tripathy S,Mohanty S,et al.Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27with efficient utilization of hydroxylamine[J].Bioresource Technology,2017,232:285-296.
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[24]王文超.重金属冲击对异养硝化-好氧反硝化菌脱氮性能的影响研究[D].成都:西南交通大学,2013:19-24.Wang W C.Effect of Heavy Metal Impact on Nitrogen Removal Performance of Heterotrophic Nitrification-aerobic Denitrifier[D].Chengdu:Southwest Jiaotong University,2013.
[25]孙超越.重金属对Paracoccus versutus LYM好氧反硝化过程的影响[D].大连:大连理工大学,2014:21-22.Sun C Y.Effect of heavy metals on Paracoccus versutus LYM in Aerobic Denitrification Process[D].Dalian:Dalian University of Technology,2014.
[26]Bollmann A,Schmidt I,Saunders A M,et al.Influence of starvation on potential ammonia-oxidizing activity and amoA mRNA levels of Nitrosospira briensis[J].Applied&Environmental Microbiology,2005,71(3):1276-1282.
[2]Chen Q,Ni J.Ammonium removal by Agrobacterium sp.LAD9 capable of heterotrophic nitrification-aerobic denitrification[J].Journal of Bioscience&Bioengineering,2012,113(5):619-623.
[3]Joo H S,Hirai M,Shoda M.Piggery wastewater treatment using Alcaligenes faecalis strain No.4 with heterotrophic nitrification and aerobic denitrification[J].Water Research,2006,40(16):3029-3036.
[4]Kumwimba M N,Zhu B,Muyembe D K.Estimation of the removal efficiency of heavy metals and nutrients from ecological drainage ditches treating town sewage during dry and wet seasons[J].Environmental Monitoring&Assessment,2017,189(9):434-442.
[5]姚金玲,王海燕,于云江,等.城市污水处理厂污泥重金属污染状况及特征[J].环境科学研究,2010,23(6):696-702.Yao J L,Wang H Y,Yu Y J,et al.Pollution status and characteristics of heavy metals of sewage sludge from municipal wastewater treatment plants[J].Research of Environmental Science,2010,23(6):696-702.
[6]杨小龙,李文明,陈燕,等.一株好氧反硝化菌的分离鉴定及其除氮特性[J].微生物学报,2011,51(8):1062-1070.Yang X L,Li W M,Chen Y,et al.Isolation,identification and nitrogen removal characteristics of an aerobic denitrifier[J].J Microbiol,2011,51(8):1062-1070.
[7]Wan C,Yang X,Lee D J,et al.Aerobic denitrification by novel isolated strain using NO2--N as nitrogen source[J].Bioresource Technology,2011,102(15):7244-7248.
[8]Huang T,Lin G,Zhang H,et al.Nitrogen-removal efficiency of a novel aerobic denitrifying bacterium,Pseudomonas stutzeri strain ZF31,isolated from a drinking-water reservoir[J].Bioresource Technology,2015,196(4):209-216.
[9]Zhang Q.Research on key issues in determination of ammonia nitrogen in water and wastewater by Nessler's reagent spectrophotometry[J].Environmental Engineering,2009(1):85-89.
[10]Mahmood Q,Ping Z,Hayat Y,et al.Effects of nitrite to sulfide ratios on the performance of anoxic sulfide oxidizing reactor[J].Arabian Journal for Science&Engineering,2009,34(1A):45-54.
[11]国家环境保护总局.水和废水监测分析方法[M].第4版.北京:中国环境科学出版社,2002.State Environmental Protection Administration.Methods for Monitoring and Analysis of Water and Wastewater[M].4th Edition.Beijing:China Environmental Science Press,2002.
[12]Shi Z,Zhang Y,Zhou J,et al.Biological removal of nitrate and ammonium under aerobic atmosphere by Paracoccus versutus LYM[J].Bioresource Technology,2013,148(7):144-148.
[13]Li C,Yang J,Wang X,et al.Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24[J].Bioresource Technology,2015,182:18-25.
[14]Kim M,Jeong S Y,Su J Y.Aerobic denitrification of Pseudomonas putida AD-21 at different C/N ratios[J].Journal of Bioscience&Bioengineering,2008,106(5):498-502.
[15]Callander I J,Barford J P.Precipitation,chelation,and the availability of metals as nutrients in anaerobic digestion.I.Methodology[J].Biotechnology&Bioengineering,1983,25(8):1947-1957.
[16]Kuo W C,Parkin G F.Characterization of soluble microbial products from anaerobic treatment by molecular weight distribution and nickel-chelating properties[J].Water Research,1996,30(4):915-922.
[17]黄满红,李咏梅,顾国维,等.呼吸计量法在活性污泥系统废水特性测定中的应用[J].工业水处理,2005,25(9):58-60.Huang M H,Li Y M,Gu G W,et al.Application of respirometry techniques to the determination of wastewater chacrcteritics in activated sludge systems[J].Industrial water treatment,2005,25(9):58-60.
[18]Takaya N,Catalan-Sakairi M A B,Sakaguchi Y,et al.Aerobic denitrifying bacteria that produce low levels of nitrous oxide[J].Applied&Environmental Microbiology,2003,69(6):3152-3157.
[19]Zhang Q L,Liu Y,Ai G M,et al.The characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium,Bacillus methylotrophicus strain L7[J].Bioresource Technology,2012,108(3):35-44.
[20]Zhao B,He Y L,Zhang X F.Nitrogen removal capability through simultaneous heterotrophic nitrification and aerobic denitrification by Bacillus sp.LY[J].Environmental Technology,2010,31(4):409-416.
[21]Guo L J,Zhao B,An Q,et al.Characteristics of a novel aerobic denitrifying bacterium,Enterobacter cloacae strain HNR[J].Applied Biochemistry&Biotechnology,2016,178(5):947-959.
[22]Padhi S K,Tripathy S,Mohanty S,et al.Aerobic and heterotrophic nitrogen removal by Enterobacter cloacae CF-S27with efficient utilization of hydroxylamine[J].Bioresource Technology,2017,232:285-296.
[23]Sun Z,Lyu Y,Liu Y,Ren R.Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp.S1[J].Bioresource Technology,2016,220:142-150.
[24]王文超.重金属冲击对异养硝化-好氧反硝化菌脱氮性能的影响研究[D].成都:西南交通大学,2013:19-24.Wang W C.Effect of Heavy Metal Impact on Nitrogen Removal Performance of Heterotrophic Nitrification-aerobic Denitrifier[D].Chengdu:Southwest Jiaotong University,2013.
[25]孙超越.重金属对Paracoccus versutus LYM好氧反硝化过程的影响[D].大连:大连理工大学,2014:21-22.Sun C Y.Effect of heavy metals on Paracoccus versutus LYM in Aerobic Denitrification Process[D].Dalian:Dalian University of Technology,2014.
[26]Bollmann A,Schmidt I,Saunders A M,et al.Influence of starvation on potential ammonia-oxidizing activity and amoA mRNA levels of Nitrosospira briensis[J].Applied&Environmental Microbiology,2005,71(3):1276-1282.