理气散结颗粒对肝气郁结模型大鼠的改善作用研究
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摘要
目的:考察理气散结颗粒对肝气郁结模型大鼠的改善作用。方法:将80只大鼠按体质量随机分为空白对照组(生理盐水)、模型对照组(生理盐水)、逍遥丸对照组(阳性对照a,以生药量计为750 mg/kg)、小金丸对照组(阳性对照b,以丸质量计为200mg/kg)、理气散结丸对照组(原型对照,以生药量计为1 957 mg/kg)和理气散结颗粒低、中、高剂量组(以生药量计分别为978.5、1 957、3 914 mg/kg),每组10只。各组大鼠均每天灌胃给药1次,灌胃体积均为20 mL/kg,连续给药21 d;每次给药1 h后,除空白对照组外的其余各组大鼠均采用束缚法复制肝气郁结模型。通过设计糖水偏嗜度实验测定大鼠的糖水偏嗜度、鼠尾悬吊实验测定大鼠悬吊不动时间和挣扎次数、旷场行为学实验测定大鼠行为总得分判断大鼠的肝郁程度以及药物的作用效果。结果:与空白对照组比较,模型对照组大鼠的悬吊不动时间显著延长,糖水偏嗜度和旷场行为学实验中行为总得分显著降低,差异均有统计学意义(P<0.05或P<0.01)。与模型对照组比较,小金丸对照组、理气散结丸对照组和理气散结颗粒中剂量组大鼠的挣扎次数显著增加(P<0.05或P<0.01);逍遥丸对照组、小金丸对照组、理气散结丸对照组和理气散结颗粒低、中剂量组大鼠的悬吊不动时间显著缩短,糖水偏嗜度和旷场行为学实验中行为总得分显著升高(P<0.05或P<0.01)。与理气散结丸对照组比较,仅理气散结颗粒高剂量组大鼠的挣扎次数显著减少,悬吊不动时间显著延长(P<0.05或P<0.01),理气散结颗粒低、中剂量组大鼠上述指标差异均无统计学意义(P>0.05)。结论:理气散结颗粒对束缚法致肝气郁结模型大鼠有明显的改善作用,且低、中剂量理气散结颗粒与理气散结丸的作用效果相当。
OBJECTIVE:To investigate the improvement effects of Liqi sanjie granule on liver-qi stagnation model rats.METHODS:According to the weight,totally 80 rats were randomly divided into blank control group(normal saline),modelcontrol group(normal saline),Xiaoyao pill control group(positive control a,750 mg/kg,calculated by crude drug),Xiaojin pillcontrol group(positive control b,200 mg/kg,calculated by pill weight),Liqi sanjie pill control group(prototype control,1 957 mg,calculated by crude drug)and Liqi sanjie granule low-dose,medium-dose and high-dose groups(978.5,1 957,3 914 mg/kg,calculated by crude drug),with 10 rats in each group. Each group was given medicine 20 mL/kg intragastrically once a day,forconsecutive 21 d. 1 h after per medication,liver-qi stagnation model was established in those groups by binding method except forblank control group. The syrup preference of rats was determined by designing syrup preference test. Rattail suspension test wasadopted to determine the hanging immobility time and struggling times of mice. Open-field behavior test was used to determinetotal behavior score so as to judge the extent of liver-qi stagnation and effect of the drug in rats. RESULTS:Compared with blankcontrol group,hanging immobility time of model control group was significantly prolonged,the syrup preference and the totalbehavior score of open field test were decreased significantly,with statistical significance(P<0.05 or P<0.01). Compared withmodel control group,the struggling times of rats were increased significantly in Xiaojin pill control group,Liqi sanjie pill controlgroup and Liqi sanjie granule medium-dose group(P<0.05 or P<0.01);the hanging immobility time of Xiaoyao pill controlgroup,Xiaojin pill control group,Liqi sanjie pill control group,Liqi sanjie granule low-dose and medium-dose groups wereshortened significantly;syrup preference and total behavior score of open-field behavior test were increased significantly(P<0.05 or P<0.01). Compared with Liqi sanjie pill control group,the struggling times of rats were decreased significantly and hangingimmobility time were prolonged significantly only in Liqisanjie granule high-dose group(P<0.05 or P<0.01);therewas no statistical significance in above indexes of rats in Liqisanjie granule low-dose and medium-dose groups(P>0.05).CONCLUSIONS: Liqi sanjie granule can significantlyimprove liver-qi stagnation caused by binding method,and theeffects of low-dose and medium-dose Liqi sanjie granule are similar to those of Liqi sanjie pill.
OBJECTIVE:To investigate the improvement effects of Liqi sanjie granule on liver-qi stagnation model rats.METHODS:According to the weight,totally 80 rats were randomly divided into blank control group(normal saline),modelcontrol group(normal saline),Xiaoyao pill control group(positive control a,750 mg/kg,calculated by crude drug),Xiaojin pillcontrol group(positive control b,200 mg/kg,calculated by pill weight),Liqi sanjie pill control group(prototype control,1 957 mg,calculated by crude drug)and Liqi sanjie granule low-dose,medium-dose and high-dose groups(978.5,1 957,3 914 mg/kg,calculated by crude drug),with 10 rats in each group. Each group was given medicine 20 mL/kg intragastrically once a day,forconsecutive 21 d. 1 h after per medication,liver-qi stagnation model was established in those groups by binding method except forblank control group. The syrup preference of rats was determined by designing syrup preference test. Rattail suspension test wasadopted to determine the hanging immobility time and struggling times of mice. Open-field behavior test was used to determinetotal behavior score so as to judge the extent of liver-qi stagnation and effect of the drug in rats. RESULTS:Compared with blankcontrol group,hanging immobility time of model control group was significantly prolonged,the syrup preference and the totalbehavior score of open field test were decreased significantly,with statistical significance(P<0.05 or P<0.01). Compared withmodel control group,the struggling times of rats were increased significantly in Xiaojin pill control group,Liqi sanjie pill controlgroup and Liqi sanjie granule medium-dose group(P<0.05 or P<0.01);the hanging immobility time of Xiaoyao pill controlgroup,Xiaojin pill control group,Liqi sanjie pill control group,Liqi sanjie granule low-dose and medium-dose groups wereshortened significantly;syrup preference and total behavior score of open-field behavior test were increased significantly(P<0.05 or P<0.01). Compared with Liqi sanjie pill control group,the struggling times of rats were decreased significantly and hangingimmobility time were prolonged significantly only in Liqisanjie granule high-dose group(P<0.05 or P<0.01);therewas no statistical significance in above indexes of rats in Liqisanjie granule low-dose and medium-dose groups(P>0.05).CONCLUSIONS: Liqi sanjie granule can significantlyimprove liver-qi stagnation caused by binding method,and theeffects of low-dose and medium-dose Liqi sanjie granule are similar to those of Liqi sanjie pill.
引文
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[5]程洪燕,梅妍,杜宏伟.柴胡舒肝散联合氯硝西泮治疗肝气郁结型焦虑症的临床观察[J].中国药房,2016,27(17):2407-2409.
[6]陈栘,徐海霞,朱明月,等.慢性低强度应激抑郁症模型大鼠组织中5-羟色胺和色氨酸羟化酶的表达变化[J].中国药房,2017,28(22):3061-3064.
[7]付义.女性肝郁患者心身状态初探及畅郁方药效作用实验研究[D].北京:北京中医药大学,2005.
[8]陈浮芸.《金匮要略》调理肝脾法在乳腺病中的运用:加味当归芍药散对乳腺增生模型动物LH、FSH、PRL水平及乳腺、子宫、卵巢组织的影响[D].福州:福建中医学院,2008.
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[11]四川省食品药品监督管理局.四川省医疗机构制剂研究技术指南(试行版)[S]. 2015-04-17.
[12]李仪奎.中药药理实验方法学[M]. 2版.上海:上海科学技术出版社,2006:543-545.
[13]周国儿,吴静,黄云娟,等.“肝郁气滞”及“肝郁脾虚”型抑郁症动物模型建立初探[J].中华中医药学刊,2014,32(5):1035-1038.
[14]萧闵,周艳艳.不同肝气郁结动物模型行为学指标的对比研究[J].中国中医药科技,2015,22(2):123-132.
[15]刘梅,周海松,许锦文,等.藜欢解郁胶囊的抗抑郁作用[J].中成药,2016,38(6):1383-1387.
[16]刘林,王宇红,孟盼,等.百事乐胶囊抗抑郁作用的实验研究[J].中成药,2014,36(8):1756-1758.
[17]谭攀攀,刘蒙,李秀英,等.西酞普兰联合石杉碱甲对老年抑郁模型大鼠的改善作用[J].中国药房,2017,28(28):3923-3926.
[18]宗阳,朱立静,孙冰婷,等.利血平诱导的抑郁模型的研究进展[J].中国药房,2016,27(19):2697-2699.
[19]保芸,张汝学,周珺,等.逍遥丸对慢性应激加高脂模型大鼠HPA轴及糖脂代谢的影响[J].中药材,2013,36(8):1309-1312.
[20]孙利昆,柳芳,陈文倩,等.基于Meta分析的小金丸治疗乳腺增生疗效评价[J].中国药物评价,2016,33(5):448-451.
[21]王珑,张迪,田旭升,等.电针对慢性应激抑郁大鼠行为学及其海马CA3区超微结构的影响[J].针灸临床杂志,2016,32(7):66-69.
[22]刘子旺,赵海滨,张秀静,等.电针肝俞、期门对肝气郁结模型大鼠行为学及HPA轴相关激素的影响[J].针灸临床杂志,2011,27(12):46-48.
[23]刘玥芸,郭晓玲,赵宏波,等.逍遥散提取物对肝郁脾虚证模型大鼠疗效的行为学评价[J].中华中医药杂志,2013,28(7):2138-2141.
[2]陈胜会,李春鹏,龚洁芹.养血清脑颗粒抗抑郁作用的实验观察[J].中成药,2015,37(1):202-204.
[3]王梅.肝为刚脏辨析[J].山东中医药大学学报,2016,40(1):23-24.
[4]尚立芝,季书,王琦,等.柴胡疏肝散对肝郁性乳腺增生模型大鼠的保护作用[J].中国药房,2015,26(7):908-911.
[5]程洪燕,梅妍,杜宏伟.柴胡舒肝散联合氯硝西泮治疗肝气郁结型焦虑症的临床观察[J].中国药房,2016,27(17):2407-2409.
[6]陈栘,徐海霞,朱明月,等.慢性低强度应激抑郁症模型大鼠组织中5-羟色胺和色氨酸羟化酶的表达变化[J].中国药房,2017,28(22):3061-3064.
[7]付义.女性肝郁患者心身状态初探及畅郁方药效作用实验研究[D].北京:北京中医药大学,2005.
[8]陈浮芸.《金匮要略》调理肝脾法在乳腺病中的运用:加味当归芍药散对乳腺增生模型动物LH、FSH、PRL水平及乳腺、子宫、卵巢组织的影响[D].福州:福建中医学院,2008.
[9]张仲景.伤寒论[M].北京:中国书店出版社,1993:39.
[10]张光仁,肖成,王育,等.理气散结丸显微与薄层色谱鉴别研究[J].内蒙古中医药,2013,32(18):145-146.
[11]四川省食品药品监督管理局.四川省医疗机构制剂研究技术指南(试行版)[S]. 2015-04-17.
[12]李仪奎.中药药理实验方法学[M]. 2版.上海:上海科学技术出版社,2006:543-545.
[13]周国儿,吴静,黄云娟,等.“肝郁气滞”及“肝郁脾虚”型抑郁症动物模型建立初探[J].中华中医药学刊,2014,32(5):1035-1038.
[14]萧闵,周艳艳.不同肝气郁结动物模型行为学指标的对比研究[J].中国中医药科技,2015,22(2):123-132.
[15]刘梅,周海松,许锦文,等.藜欢解郁胶囊的抗抑郁作用[J].中成药,2016,38(6):1383-1387.
[16]刘林,王宇红,孟盼,等.百事乐胶囊抗抑郁作用的实验研究[J].中成药,2014,36(8):1756-1758.
[17]谭攀攀,刘蒙,李秀英,等.西酞普兰联合石杉碱甲对老年抑郁模型大鼠的改善作用[J].中国药房,2017,28(28):3923-3926.
[18]宗阳,朱立静,孙冰婷,等.利血平诱导的抑郁模型的研究进展[J].中国药房,2016,27(19):2697-2699.
[19]保芸,张汝学,周珺,等.逍遥丸对慢性应激加高脂模型大鼠HPA轴及糖脂代谢的影响[J].中药材,2013,36(8):1309-1312.
[20]孙利昆,柳芳,陈文倩,等.基于Meta分析的小金丸治疗乳腺增生疗效评价[J].中国药物评价,2016,33(5):448-451.
[21]王珑,张迪,田旭升,等.电针对慢性应激抑郁大鼠行为学及其海马CA3区超微结构的影响[J].针灸临床杂志,2016,32(7):66-69.
[22]刘子旺,赵海滨,张秀静,等.电针肝俞、期门对肝气郁结模型大鼠行为学及HPA轴相关激素的影响[J].针灸临床杂志,2011,27(12):46-48.
[23]刘玥芸,郭晓玲,赵宏波,等.逍遥散提取物对肝郁脾虚证模型大鼠疗效的行为学评价[J].中华中医药杂志,2013,28(7):2138-2141.