高良姜水提物的体外抗氧化和胰脂肪酶、α-葡萄糖苷酶抑制功能研究
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摘要
以新鲜高良姜为原料,经冻干脱脂处理制备水提物,测定高良姜水提物中多酚和黄酮含量,使用ABTS法、DPPH法和FRAP法评价水提物的抗氧化能力,同时测定其对α-葡萄糖苷酶和胰脂肪酶的抑制功能。结果显示:高良姜水提物中多酚和总黄酮分别为45.73mg GAE/g DW和0.41mg QE/g DW。水提物对ABTS和DPPH自由基离子的半抑制浓度IC50分别为2.55,8.78 mg/mL。FRAP值为382.14μmol Fe2+/g DW。高良姜水提物对α-葡萄糖苷酶和胰脂肪酶抑制活性的IC50值分别为14.45,543.51mg/mL。表明高良姜水提物中含有丰富的多酚类化合物,具有较强的体外抗氧化能力和α-葡萄糖苷酶和胰脂肪酶抑制功能。
Take fresh galangal as the raw material,it is freeze-dried,degreased and extracted by pure water,the content of polyphenols and flavonoids in galangal aqueous extracts is detected,the antioxidant capacities of galangal aqueous extracts are evaluated by ABTS method,DPPH method,FRAP method,and the inhibitory activities against pancreatic lipase andα-glucosidase are detected.The results show that the content of polyphenols and total flavonoids is 45.73 mg GAE/g DW and0.41 mg QE/g DW respectively.The aqueous extracts of galangal could scavenge ABTS and DPPH radicals effectively with IC50 of 2.55,8.78 mg/mL respectively.The FRAP value of the aqueous extracts is 382.14μmol Fe2+/g DW.The inhibitory activities againstα-glucosidase and pancreatic lipase expressed by IC50 is 14.45,543.51 mg/mL respectively.In summary,the galangal is abundant in polyphenols,and has stronger antioxidant activities and inhibitory activities againstα-glucosidase and pancreatic lipase.
Take fresh galangal as the raw material,it is freeze-dried,degreased and extracted by pure water,the content of polyphenols and flavonoids in galangal aqueous extracts is detected,the antioxidant capacities of galangal aqueous extracts are evaluated by ABTS method,DPPH method,FRAP method,and the inhibitory activities against pancreatic lipase andα-glucosidase are detected.The results show that the content of polyphenols and total flavonoids is 45.73 mg GAE/g DW and0.41 mg QE/g DW respectively.The aqueous extracts of galangal could scavenge ABTS and DPPH radicals effectively with IC50 of 2.55,8.78 mg/mL respectively.The FRAP value of the aqueous extracts is 382.14μmol Fe2+/g DW.The inhibitory activities againstα-glucosidase and pancreatic lipase expressed by IC50 is 14.45,543.51 mg/mL respectively.In summary,the galangal is abundant in polyphenols,and has stronger antioxidant activities and inhibitory activities againstα-glucosidase and pancreatic lipase.
引文
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[20]Garza A L,Milagro F I,Boque N N,et al.Natural inhibitors of pancreatic lipase as new players in obesity treatment[J].Planta Medica,2011,77(8):773-785.
[21]Marrelli M,Loizzo M R,Nicoletti M,et al.In vitro investigation of the potential health benefits of wild Mediterranean dietary plants as anti-obesity agents withα-amylase and pancreatic lipase inhibitory activities[J].Journal of the Science of Food and Agriculture,2014,94(11):2217-2224.
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[3]Basri A M,Taha H,Ahmad N.A review on the pharmacological activities and phytochemicals of Alpinia officinarum(galangal)extracts derived from bioassay-guided fractionation and isolation[J].Pharmacognosy Reviews,2017,11(21):43-56.
[4]汪光华,唐树平,彭名军.高良姜中4种黄酮化合物的体外抗氧化能力及抑菌活性研究[J].食品与机械,2017,33(5):168-172.
[5]He F,Li J,Liu Z,et al.Redox mechanism of reactive oxygen species in exercise[J].Frontier in Physiology,2016(7):486.
[6]周昇昇,杨月欣.抗氧化能力体外评价方法的进展和比较[J].卫生研究,2010,39(2):164-167.
[7]Obrenovich M E,Li Y,Parvathaneni K,et al.Antioxidants in health,disease and aging[J].CNS&Neurological Disorders Drug Targets,2011,10(2):192-207.
[8]Rajendran P,Nandakumar N,Rengarajan T,et al.Antioxidants and human diseases[J].Clinica Chimica Acta,2014,436:332-347.
[9]Ainsworth E A,Gillespie K M.Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent[J].Nature Protocols,2007(4):875-877.
[10]Arvouet Grand A,Vennat B,Pourrat A,et al.Standardization of propolis extract and identification of principal constituents[J].Journal de Pharmacie de Belgique,1994,49(6):462-468.
[11]Ali M Y,Jung H J,Jannat S,et al.In vitro antidiabetic and antioxidant potential of the ethanolic extract of skipjack tuna(Katsuwonus pelamis)heart[J].Journal of Food Biochemistry,2016,40(4):583-592.
[12]Cheng Z H,Moore J,Yu L L.High-throughput relative DPPH radical scavenging capacity assay[J].Journal of Agricultural and Food Chemistry,2006,54(20):7429-7436.
[13]Sreerama Y N,Takahashi Y,Yamaki K.Phenolic antioxidants in some vigna species of legumes and their distinct inhibitory effects on alpha-glucosidase and pancreatic lipase activities[J].Journal of Food Science,2012,77(9):927-933.
[14]Kang W Y,Song Y L,Zhang L.α-Glucosidase inhibitory and antioxidant properties and antidiabetic activity of Hypericum ascyron L.[J].Medicinal Chemistry Research,2010,20(7):809-816.
[15]McDougall G J,Kulkarni N N,Stewart D.Berry polyphenols inhibit pancreatic lipase activity in vitro[J].Food Chemistry,2009,115(1):193-199.
[16]杨少辉,宋英今,王清华,等.雪莲果体外抗氧化和自由基清除能力[J].食品科学,2010,31(17):166-169.
[17]石雪萍,李小华,杨爱萍.高良姜中总黄酮提取与DPPH自由基清除活性研究[J].中国调味品,2012,37(6):53-56.
[18]郭长江,杨继军,李云峰,等.FRAP法测定水果不同部分抗氧化活性[J].中国公共卫生,2003,19(7):841-843.
[19]Proenca C,Freitas M,Ribeiro D,et al.α-Glucosidase inhibition by flavonoids:an in vitro and in silico structure-activity relationship study[J].Journal of Enzyme Inhibition and Medicinal Chemistry,2017,32(1):1216-1228.
[20]Garza A L,Milagro F I,Boque N N,et al.Natural inhibitors of pancreatic lipase as new players in obesity treatment[J].Planta Medica,2011,77(8):773-785.
[21]Marrelli M,Loizzo M R,Nicoletti M,et al.In vitro investigation of the potential health benefits of wild Mediterranean dietary plants as anti-obesity agents withα-amylase and pancreatic lipase inhibitory activities[J].Journal of the Science of Food and Agriculture,2014,94(11):2217-2224.