以介孔二氧化硅为固定相的高性能微色谱柱
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摘要
基于微机电系统(Micro-electro-mechanical system, MEMS)技术设计制造了一种含有椭圆微柱阵列的半填充微色谱柱,此微色谱柱内壁以孔径约2 nm、具有大比表面积的介孔二氧化硅(Mesoporous silica,MS)薄膜为固定相。在相同进样量下,采用程序升温模式进行测试,以介孔二氧化硅为固定相的微色谱柱,可实现烷烃(C5～C10)的基线分离,其中戊烷与溶剂(杂质)峰分离度达3.6,与以二甲基硅油(Polydimethylsiloxane,PDMS)为固定相的微色谱柱相比,辛烷理论塔板数提高了46%,可达14458 plates,壬烷的峰面积提高了349.8%。
A micro-fabricated semi-packed gas chromatography(μGC) column embedded micro-elliptical pillars was fabricated based on micro-electro-mechanical system(MEMS) technique. The mesoporous silica film(MS) with 2 nm pore size and large specific surface area was coated on the inner surface of the μGC column as stationary phase. Under the same injection volume and the temperature-programmed conditions, the baseline separation of alkanes(C5-C10) was realized by the μGC column used MS as stationary phase, and the resolution of pentane and solvent(impurity) could be as large as 3.6. Compared with the GC column used polydimethylsiloxane(PDMS) as stationary phase, the number of theoretical plates of octane had a 46% increase, which could be up to 14458 plates. Meanwhile, the peak area of nonane increased by 349.8%.
A micro-fabricated semi-packed gas chromatography(μGC) column embedded micro-elliptical pillars was fabricated based on micro-electro-mechanical system(MEMS) technique. The mesoporous silica film(MS) with 2 nm pore size and large specific surface area was coated on the inner surface of the μGC column as stationary phase. Under the same injection volume and the temperature-programmed conditions, the baseline separation of alkanes(C5-C10) was realized by the μGC column used MS as stationary phase, and the resolution of pentane and solvent(impurity) could be as large as 3.6. Compared with the GC column used polydimethylsiloxane(PDMS) as stationary phase, the number of theoretical plates of octane had a 46% increase, which could be up to 14458 plates. Meanwhile, the peak area of nonane increased by 349.8%.
引文
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2 XU Jian-Ling,SHENG Lian-Xi,WANG Han-Xi,LIU Yuan-Yuan,ZHANG Tian.Chinese J.Anal.Chem.,2014,42(10):1513-1517徐建玲,盛连喜,王汉席,刘元园,张田.分析化学,2014,42(10):1513-1517
3 ZHAO Xiao-Ya,WANG Yue-Fei,YANG Fan,JIANG Zhen-Zuo,WANG Yan,YANG Long,PAN Zan-Hong.Chinese J.Anal.Chem.,2016,44(7):1009-1014赵晓亚,王跃飞,杨帆,江振作,王琰,杨龙,潘赞红.分析化学,2016,44(7):1009-1014
4 Overton E B,Dharmasena H P,Ehrmann U,Carney K R.Field Anal.Chem.Tech.,2015,1(2):87-92
5 Haghighi F,Talebpour Z,Sanati-Nezhad A.Lab Chip,2015,15(12):2559-2575
6 Alfeeli B,Narayanan S,Moodie D,Zellner P,McMillan M,Hirtenstein D,Rice G,Agah M.IEEE Sens.J.,2013,13(11):4312-4319
7 Regmi B P,Agah M.Anal.Chem.,2018,90(22):13133-13150
8 Sun J,Cui D,Chen X,Zhang L,Cai H,Li H.J.Chromatogr.A,2013,1291:122-128
9 Li Y,Du X,Wang Y,Tai H,Qiu D,Lin Q,Jiang Y.RSC Adv.,2014,4(8):3742-3747
10 Bhushan A,Yemane D,Overton E B,Goettert J,Murphy M C.J.Microelectromech.Syst.,2007,16(2):383-393
11 Zellner P,Renaghan L,Hasnain Z,Agah M.J.Micromech.Microeng.,2010,20(4):045013
12 Terry S C,Jerman J H,Angell J B.IEEE T Electron Dev.,1979,26(12):1880-1886
13 Michael S,Mcbrady A D,Brian D,Reid V R,Aleksandr N,Synovec R E,Olgica B.Anal.Chem.,2006,78(16):5639-5644
14 Agah M,Wise K D.J.Microelectromech.Syst.,2007,16(4):853-860
15 Alfeeli B,Narayanan S,Mcmillan M,Hirtenstein D,Rice G,Agah M.In Proc.IEEE Sens.,2011,25(35):1097-1100
16 Azzouz I,Vial J,Thiébaut D,Haudebourg R,Danaie K,Sassiat P,Breviere J.Anal.Bioanal.Chem.,2013,406(4):981-994
17 Ali S,Ashraf-Khorassani M,Taylor L T,Agah M.Sens.Actuators B,2009,141(1):309-315
18 Shakeel H,Agah M.Sens.Actuators B,2017,242:215-223
19 Tian B,Zhao B,Feng F,Luo F,Zhou H,Ge X,Wu Y,Li X.J.Chromatogr.A,2018,1565:130-137
20 Shakeel H,Agah M.J.Microelectromech.Syst.,2013,22(1):62-70
21 Dong W,Hamza S,John L,Rice G W,Heflin J R,Masoud A.Anal.Chem.,2013,85(17):8135-8141
22 Haudebourg R,Vial J,Thiebaut D,Danaie K,Breviere J,Sassiat P,Azzouz I,Bourlon B.Anal.Chem.,2013,85(1):114-120
23 TIAN Hua-Jun,XUE Yun,CHEN Qiao-Mei,WANG Yan,YAN Chao.Chinese Journal of Chromatography,2017,35(5):473-481田华君,薛芸,陈巧梅,王彦,阎超.色谱,2017,35(5):473-481
24 Luo F,Zhao B,Feng F,Hou L,You W,Xu P,Zhou H,Li X.Talanta,2018,188:546-551
25 Hou L,Feng F,You W,Xu P,Luo F,Tian B,Zhou H,Li X.J.Chromatogr.A,2018,1552:73-78