意大利牛舌草抗氧化及抗血栓作用研究
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摘要
目的研究意大利牛舌草水、乙醇提取物抗氧化及抗血栓的作用。方法将意大利牛舌草粉碎成粗粉,分别用蒸馏水和70%乙醇提取,得到意大利牛舌草水提取物和乙醇提取物。选取健康昆明小鼠70只,随机分为7组(n=10),正常对照组给予等体积蒸馏水,意大利牛舌草水提物高(678.40mg/kg)、中(339.20mg/kg)、低剂量组(169.60mg/kg),意大利牛舌草乙醇提物高(409.50mg/kg)、中(204.75mg/kg)、低剂量组(102.38mg/kg),各组小鼠每天灌胃给药1次,连续灌胃21d,按试剂盒方法说明书测定小鼠血清中的SOD、MDA、GSH-PX含量。测定意大利牛舌草水、乙醇提取物对正常小鼠凝血时间、止血时间及对正常大鼠动脉血栓形成的作用。结果与正常对照组比较,意大利牛舌草乙醇提物高剂量组小鼠血清中SOD、GSH-PX活性升高,MDA含量降低,差异有统计学意义(P <0.01);意大利牛舌草乙醇提物中剂量和低剂量组小鼠血清中SOD、GSH-PX活性升高,MDA含量降低,差异有统计学意义(P <0.05);意大利牛舌草水提物各剂量组小鼠血清中SOD、GSH-PX活性升高,MDA含量降低,差异无统计学意义(P>0.05)。意大利牛舌草乙醇提物(204.75mg/kg)和水提物(339.20mg/kg)均可延长正常小鼠的凝血时间,意大利牛舌草乙醇提物(141.75mg/kg)和水提物(234.83mg/kg)均可降低大鼠血栓湿重,血栓抑制率分别为42.76%、36.68%。结论意大利牛舌草乙醇提取物具有抗氧化作用,意大利牛舌草水、乙醇提取物可以延长凝血时间,防止血栓的形成。
Objective To study the antioxidant and antithrombotic effects of water and ethanol extracts of Anchusa italica Retiz.Methods Anchusa italica Retiz.was crushed into crude powder and extracted with distilled water and 70% ethanol respectively.The water extract and ethanol extract of Anchusa italica Retiz.were obtained.70 healthy Kunming mice were randomly divided into 7groups(n =10).The control group was given distilled water.There were high-dose(678.40mg/kg),medium-dose(339.20mg/kg),low-dose(169.60 mg/kg)of water extraction groups,and high-dose(409.50 mg/kg),medium-dose(204.75mg/kg),and low-dose(102.38 mg/kg)of ethanol extraction groups.The dosage was given to mice for 21 days.The level of SOD,MDA and GSH-PX in the serum of mice were determined according to the kit instructions.The effects of water and ethanol extractions of Anchusa italica Retiz.on coagulation time,hemostasis time and arterial thrombosis in normal mice were examined.Results Compared with the control group,there were an increase on the level of SOD and GSH-PX and decrease on MDA in high dose ethanol extraction group,and the differences were statistically significant(P <0.01).The level of SOD and GSH-PX in serum of mice in medium-dose and low-dose of ethanol extraction group increased and the level of MDA decreased,and the differences were statistically significant(P <0.05).The level of SOD and GSH-PX in serum of mice from each dose group of water extraction groups were increased,and the content of MDA was decreased,but the difference was not statistically significant(P >0.05).Ethanol extraction(204.75mg/kg)and water extraction(339.20mg/kg)can significantly prolong the clotting time of blood.Both ethanol extraction(141.75mg/kg)and water extraction(234.83mg/kg)of Anchusa italica Retiz.could reduce the wet weight of thrombus in rats,and the thrombus inhibition rates were 42.76%and 36.68%,respectively.Conclusion The ethanol extract of Anchusa italica Retiz.has anti-oxidation effect,and the extraction of water and ethanol can significantly prolong the clotting time and prevent the thrombosis.
Objective To study the antioxidant and antithrombotic effects of water and ethanol extracts of Anchusa italica Retiz.Methods Anchusa italica Retiz.was crushed into crude powder and extracted with distilled water and 70% ethanol respectively.The water extract and ethanol extract of Anchusa italica Retiz.were obtained.70 healthy Kunming mice were randomly divided into 7groups(n =10).The control group was given distilled water.There were high-dose(678.40mg/kg),medium-dose(339.20mg/kg),low-dose(169.60 mg/kg)of water extraction groups,and high-dose(409.50 mg/kg),medium-dose(204.75mg/kg),and low-dose(102.38 mg/kg)of ethanol extraction groups.The dosage was given to mice for 21 days.The level of SOD,MDA and GSH-PX in the serum of mice were determined according to the kit instructions.The effects of water and ethanol extractions of Anchusa italica Retiz.on coagulation time,hemostasis time and arterial thrombosis in normal mice were examined.Results Compared with the control group,there were an increase on the level of SOD and GSH-PX and decrease on MDA in high dose ethanol extraction group,and the differences were statistically significant(P <0.01).The level of SOD and GSH-PX in serum of mice in medium-dose and low-dose of ethanol extraction group increased and the level of MDA decreased,and the differences were statistically significant(P <0.05).The level of SOD and GSH-PX in serum of mice from each dose group of water extraction groups were increased,and the content of MDA was decreased,but the difference was not statistically significant(P >0.05).Ethanol extraction(204.75mg/kg)and water extraction(339.20mg/kg)can significantly prolong the clotting time of blood.Both ethanol extraction(141.75mg/kg)and water extraction(234.83mg/kg)of Anchusa italica Retiz.could reduce the wet weight of thrombus in rats,and the thrombus inhibition rates were 42.76%and 36.68%,respectively.Conclusion The ethanol extract of Anchusa italica Retiz.has anti-oxidation effect,and the extraction of water and ethanol can significantly prolong the clotting time and prevent the thrombosis.
引文
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[2]徐晓娜,牛子冉,王守宝,等.牛舌草总黄酮抗大鼠心肌缺血再灌注损伤的作用及机制[J].药学学报,2014,49(6):875-881.
[3]何媛媛,王金凤,郭丽娜,等.意大利牛舌草化学成分及药理作用研究进展[J].环球中医药,2017,10(1):114-120.
[4]郭朝峰,穆玉明.三氯化铁动物血栓模型方法学及其应用研究进展[J].中华实用诊断与治疗杂志,2010,24(6):537-539.
[5]张海英,盛树东,薛洁,等.三七止血与抗血栓作用的实验研究[J].新疆医科大学学报,2012,35(4):487-490.
[6]罗琼,金红,谭学瑞.血栓形成机制及治疗进展[J].心血管康复医学杂志,2008,17(1):83-86.
[7]王新玲.新疆鼠尾草抗血栓作用物质基础及其作用机制研究[D].乌鲁木齐:新疆医科大学,2014.
[8]王虹,刘敏,顾建祥,等.迷迭香酸对D-半乳糖致衰老小鼠抗氧化作用的研究[J].中国老年学杂志,2009,9(5):549-551.
[9]阳冠明,孙胜涛,李树全,等.S-甲基异硫脲对阿霉素致大鼠心肌组织超氧化物歧化酶、谷胱甘肽过氧化物酶活性改变的影响[J].中国病理生理杂志,2006,22(12):2349-2354.
[10]谢周建,戴沩,黎梅,等.牛舌草活性部位的抗氧化和美白作用[J].中国实验方剂学杂志,2014,20(12):150-152.
[11]朱庆磊,吕欣然,何爱霞.葛根素对氧自由基的清除和抗氧化性损伤作用[J].解放军药学学报,2001,17(1):1-4.
[12]刘方,武子斌,牛淑敏.中药材抗氧化及自由基清除活性的研究[J].中国药学杂志,2001,36(7):442-445.
[13]杨少辉,宋英今,王洁华,等.雪莲果体外抗氧化和自由基清除能力[J].食品科学,2010,31(17):166-169.