广谱抗稻瘟病种质75-1-127的褐飞虱抗性基因鉴定及分子标记辅助选择育种
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摘要
【目的】水稻品系75-1-127携带广谱抗稻瘟病基因Pi9,已被广泛应用于抗稻瘟病水稻品种改良。笔者育种实践发现75-1-127表现出较强的褐飞虱抗性,因此鉴定该品系中的褐飞虱抗性基因并进行分子辅助选择育种。【方法】根据水稻品系B5中褐飞虱抗性基因Bph14和Bph15的序列,设计引物扩增75-1-127的基因组DNA,并对PCR产物进行测序分析。采用苗期集团法鉴定了75-1-127和B5的褐飞虱抗性表型。利用与Bph14与Bph15连锁的分子标记筛查了75-1-127为稻瘟病抗源回交转育的两系不育系后代,并鉴定了这些后代的稻瘟病抗性、褐飞虱抗性和主要农艺性状。【结果】75-1-127中含有与B5完全一致的Bph14和Bph15序列。75-1-127和B5苗期褐飞虱抗性均为1级。在以75-1-127为抗源改良的两系不育系中,携带Bph14、Bph15的单基因系或双基因系的褐飞虱抗性均得以改良,其中双基因聚合系的死苗率为8.5%,与供体亲本75-1-127以及阳性对照B5差异不显著,进一步证实75-1-127含有褐飞虱抗性基因。【结论】水稻品系75-1-127携带褐飞虱抗性基因Bph14和Bph15,可以作为抗源应用于水稻褐飞虱抗性育种。
【Objective】Rice line, 75-1-127, which harbors the broad-spectrum blast resistance gene Pi9, has been widely used in rice variety improvement for blast resistance. Given that 75-1-127 shows strong resistance to brown planthoppers in our previous breeding projects, we identified its resistance genes to brown planthoppers and used it in molecular marker-assisted selection breeding. 【Method】The PCR products, which amplified from the genomic DNA of 75-1-127 with the primers designed according to the sequence of Bph14 and Bph15 genes in rice line B5, were sequenced and analyzed. The brown planthopper resistance of 75-1-127 and B5 were also identified with seedling bulk test method. A series of two-line genic male sterile lines derived from backcross with 75-1-127 as a donor parent, were screened with the molecular markers linked to Bph14 and Bph15 genes. The blast resistance, brown planthopper resistance and main agronomic traits of these progenies were also identified and evaluated. 【Result】The sequences of Bph14 and Bph15 in75-1-127 were identical with those in B5. The resistance scores at seedling stage of 75-1-127 and B5 were both grade 1.In the progenies with 75-1-127 as resistance resource, the resistances to brown planthoppers of those monogenic lines of Bph14 or Bph15 and those Bph14/Bph15 pyramiding lines were all improved. That the seedling mortality of Bph14/Bph15 pyramiding lines was 8.5%, equivalent to the donor parents 75-1-127 and the positive control B5, further confirming that 75-1-127 contained resistance genes to brown planthoppers. 【Conclusion】Rice line 75-1-127, which carries the genes of Bph14 and Bph15, could be used as resistance resource in breeding.
【Objective】Rice line, 75-1-127, which harbors the broad-spectrum blast resistance gene Pi9, has been widely used in rice variety improvement for blast resistance. Given that 75-1-127 shows strong resistance to brown planthoppers in our previous breeding projects, we identified its resistance genes to brown planthoppers and used it in molecular marker-assisted selection breeding. 【Method】The PCR products, which amplified from the genomic DNA of 75-1-127 with the primers designed according to the sequence of Bph14 and Bph15 genes in rice line B5, were sequenced and analyzed. The brown planthopper resistance of 75-1-127 and B5 were also identified with seedling bulk test method. A series of two-line genic male sterile lines derived from backcross with 75-1-127 as a donor parent, were screened with the molecular markers linked to Bph14 and Bph15 genes. The blast resistance, brown planthopper resistance and main agronomic traits of these progenies were also identified and evaluated. 【Result】The sequences of Bph14 and Bph15 in75-1-127 were identical with those in B5. The resistance scores at seedling stage of 75-1-127 and B5 were both grade 1.In the progenies with 75-1-127 as resistance resource, the resistances to brown planthoppers of those monogenic lines of Bph14 or Bph15 and those Bph14/Bph15 pyramiding lines were all improved. That the seedling mortality of Bph14/Bph15 pyramiding lines was 8.5%, equivalent to the donor parents 75-1-127 and the positive control B5, further confirming that 75-1-127 contained resistance genes to brown planthoppers. 【Conclusion】Rice line 75-1-127, which carries the genes of Bph14 and Bph15, could be used as resistance resource in breeding.
引文
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[4]Hu J,Xiao C,He Y Q.Recent progress on the genetics and molecular breeding of brown planthopper resistance in rice.Rice,2016,9:30.
[5]Pathak M D,Cheng C H,Fortuno M E.Resistance to Nephotettix impicticeps and Nilaparvata lugens in varieties of rice.Nature,1969,223:502-504.
[6]Cheng X Y,Zhu L L,He G C.Towards understanding of molecular interactions between rice and the brown planthopper.Mol Plant,2013,6:621-634.
[7]Fujita D,Kohli A,Horgan F G.Rice resistance to plant hoppers and leaf hoppers.Crit Rev Plant Sci,2013,32:162-191.
[8]Kobayashi T.Evolving ideas about genetics underlying insect virulence to plant resistance in rice-brown planthopper interactions.J Insect Physiol,2016,84:32-39.
[9]Yang L,Zhang W L.Genetic and biochemical mechanisms of rice resistance to planthopper.Plant Cell Rep,2016,35(8):1559-1572.
[10]Wang Y,Cao L M,Zhang Y X,Cao C X,Liu F,Huang FK,Qiu Y F,Li R B,Lou X J.Map-based cloning and characterization of BPH29,a B3 domain-containing recessive gene conferring brown planthopper resistance in rice.J Exp Bot,2015,66:6035-6045.
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[12]Yang L,Li R B,Li Y R,Huang F K,Chen Y Z,Huang SS,Huang L F,Liu C,Ma Z F,Huang D H,Jiang J J.Genetic mapping of bph20(t)and bph21(t)loci conferring brown planthopper resistance to Nilaparvata lugens St?l in rice(Oryza sativa L.).Euphytica,2011,183:161-171.
[13]Du B,Zhang W L,Liu B F,Hu J,Wei Z,Shi Z Y,He RF,Zhu L L,Chen R Z,Han B,He G C.Identification and characterization of Bph14,a gene conferring resistance to brown planthopper in rice.Proc Natl Acad Sci USA,2009,106:22 163-22 168.
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[15]Lv W T,Du B,Shangguan X X,Zhao Y,Pan Y F,Zhu LL,He Y Q,He G C.BAC and RNA sequencing reveal the brown planthopper resistance gene BPH15 in a recombination cold spot that mediates a unique defense mechanism.BMC Genom,2014,15:674.
[16]Tamura Y,Hattori M,Yoshioka H,Yoshioka M,Takahashi A,Wu J,Sentoku N,Yasui H.Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L.ssp.indica cultivar ADR52.Sci Rep,2014,4:5872.
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[19]Ren J S,Gao F Y,Wu X T,Lu X J,Zeng L H,Lv J Q,Su X W,Luo H,Ren G J.Bph32,a novel gene encoding an unknown SCR domain containing protein,confers resistance against the brown planthopper in rice.Sci Rep,2016,6:37645.
[20]Cheng X Y,Wu Y,Guo J P,Du B,Chen R Z,Zhu L L,He G C.A rice lectin receptor-like kinase that is involved in innate immune responses also contributes to seed germination.Plant J,2013,76:687-698.
[21]李进波,夏明元,戚华雄,何光存,万丙良,查中萍.水稻抗褐飞虱基因Bphl4和Bph15的分子标记辅助选择.中国农业科学,2006,39(10):2132-2137.Li J B,Xia M Y,Qi H X,He G C,Wan B L,Zha Z P.Marker-assisted selection for brown planthopper(Nilaparvata lugens St?l)resistance genes Bph14 and Bph15 in rice.Sci Agric Sin,2006,39(10):2132-2137.(in Chinese with English abstract)
[22]Hu J,Li X,Wu C J,Yang C J,Hua H X,Gao G J,Xiao JH,He Y Q.Pyramiding and evaluation of the brown planthopper resistance genes Bph14 and Bph15 in hybrid rice.Mol Breed,2012,29(1):61-69.
[23]阎勇,粟学俊,梁曼玲,黄凤宽,陈彩虹.抗褐飞虱基因Bph14和Bph15杂交稻的分子标记辅助选育与抗性评价.分子植物育种,2015,13(7):1450-1456.Yan Y,Su X J,Liang M L,Huang F K,Chen C H.Resistance evaluation and marker-assisted selection(MAS)for brown planthopper resistance genes Bph14and Bph15 in hybrid rice.Mol Plant Breed,2015,13(7):1450-1456.(in Chinese with English abstract)
[24]蔡之军,周德银,高荣村,王建华,李金军.水稻抗褐飞虱基因Bph14和Bph15在粳稻育种上的应用.江苏农业学报,2016,32(2):257-261.Cai Z J,Zhou D Y,Gao R C,Wang J H,Li J J.Application of brown planthopper-resistant genes Bph14and Bph15 in japonica rice breeding.Jiangsu J Agric Sci,2016,32(2):257-261.(in Chinese with English abstract)
[25]徐晓明,程攀,陈龙,曲姗姗,阴云伙,田发春,彭炳生,吴帅,李土明,周卫营.应用分子标记辅助选育抗褐飞虱水稻两系不育系.安徽农业科学,2016,44(20):107-108,213.Xu X M,Cheng P,Chen L,Qu S S,Yin Y H,Tian F C,Peng B S,Wu S,Li T M,Zhou W Y.Breeding TGMSlines with resistance to brown planthopper by marker-assisted selection.J Anhui Agric Sci,2016,44(20):107-108,213.(in Chinese with English abstract)
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