大孔吸附树脂分离纯化薰衣草中迷迭香酸
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摘要
研究不同大孔吸附树脂对薰衣草中迷迭香酸静态吸附与解吸特性,筛选出一种吸附及解吸效果良好的树脂LX-60,其静态吸附量与解吸量分别达到5.12 mg/g和4.86 mg/g。对LX-60进行吸附和解析条件考察,最终得到纯度为15.6%的迷迭香酸产品,其得率为86.79%。比纯化前的迷迭香酸质量浓度0.37%提高了42倍。本研究表明,利用大孔吸附树脂能够纯化薰衣草中的迷迭香酸,为通过薰衣草提取分离来生产迷迭香酸提供理论和实验基础。
Static adsorption and desorption of characteristics of different macroporous adsorption resin was studied,LX-60 has a good effect to adsorb and desorb. The static adsorption and desorption of quantity was 5.12 mg/g and 4.86 mg/g. And adsorption and desorption of condition was investigated for LX-60,products of rosemary acid was got in the end,purity was 15.6% and yield was 86.79%. The purity of rosemary acid was 42 times higher than the purity of 0.37%. This study indicated it was feasible to purify rosemary acid in the lavender,as well as this research provided theory and experimental basis for production.
Static adsorption and desorption of characteristics of different macroporous adsorption resin was studied,LX-60 has a good effect to adsorb and desorb. The static adsorption and desorption of quantity was 5.12 mg/g and 4.86 mg/g. And adsorption and desorption of condition was investigated for LX-60,products of rosemary acid was got in the end,purity was 15.6% and yield was 86.79%. The purity of rosemary acid was 42 times higher than the purity of 0.37%. This study indicated it was feasible to purify rosemary acid in the lavender,as well as this research provided theory and experimental basis for production.
引文
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[16]BAI Duolong,YANG Kaihua.Macroporous adsorption resin purification technology and application[J].Strait Pharmaceutical Journal,2007,19(9):96-99.(in Chinese)
[17]SUN Xing,SHENG Huagang,ZHANG Chao,et al.Influence of chitosan flocculation sedimentation method on flavonoids and alkaloids from gegen Qinlian decoction[J].Chinese Journal of Experimental Traditional Medical Formulae,2012,18(20):12-15.(in Chinese)
[2]YUAN Ganjun,LONG Lina.Separation of rosmarinic acid from rosmarinus officinalis L with macroporous absorption resin[J].Lishizhen Medicineand Materia Medica Research,2010,21(1):234-236.(in Chinese)
[3]OU Yuhang,LI Jie,CHEN Qiang.Study on separation and enrichment of rosmarinic acid from sarcandr glabra extract by macroporous resin[J].China Medical Herald,2012,9(36):128-131.(in Chinese)
[4]FONTELES,ANALU A,de Souza,et al.Rosmarinic acid prevents against memory deficits inischemic mice[J].Behavioural Brain Research,2016,297:91-103.
[5]FRANKEL E N,HUANG S W,AESCHBACH R,et al.Antioxidant activity of a rosemary extract and its constituents,carnosic acid,carnosol,and rosmarinicacid,in bulk oil and oil-in-wateremulsion[J].Journal of Agricultural and Food Chemistry,1996,44(1):131-135.
[6]OSAKABE N,YASUDA A,NATSUME M,et al.Rosmarinic acid inhibits epidermal inflammatory responses:anticarcinogenic effect of Perilla frutescens extract in the murine two-stage skin model[J].Carcinogenesis,2004,25(4):549-557.
[7]OSAKABE N,TAKANO H,SANBONGI C,et al.Anti-inflammatory and anti-allergic effect of rosmarinic acid(RA);inhibition of seasonal allergic rhinoconjunctivitis(SAR)and its mechanism[J].Biofactors,2004,21(1):127-131.
[8]LI Chenyang,ZHAO Jun,XU Fang,et al.Determination of the content of rosmarinic acid in Lavandula angustifolia[J].Northwest Pharm,2014,29(3):230-232.(in Chinese)
[9]UENO T,MATSUI Y,MASUDA H,et al.Antidepressant-like effects of 3-(3,4-dihydroxyphenyl)lactic acid isolated from lavender(Lavandula angustifolia)flowers in mice[J].Food Science and Technology Research,2014,20(6):1213-1219.
[10]PENG Qing,LI Xiaogang,LIU Yaming.Macroporous adsorption resin is reviewed[J].Journal of Practical Traditional Chinese Medicine,2013,29(5):409-412.(in Chinese)
[11]SHI Lei,TIAN Yumiao,YU Wenguo,et al.Separation and purification of 3-methylxanthine from crystallization mother liquor with macroporous adsorption resin[J].Chinese Journal of Pharmaceuticals,2015,46(12):1347-1351.(in Chinese)
[12]FU B Q,LIU J,LI H,et al.The application of macroporous resins in the separation of licorice flavonoids and glycyrrhizic acid[J].Journal of Chromatography A,2005,1089(1):18-24.
[13]LI Yingchang,ZHENG Fenge,MENG Xianjun.Studies on purification of anthocyanins from blueberry fruits by macroporous resins[J].Journal of Food Science and Biotechnology,2009,28(4):496-500.(in Chinese)
[14]QIAN Zhu,XU Peng,ZHANG Kechang,et al.Extraction of ganoderic triterpenes from fermented broth by macroporous resin[J].Journal of Food Science and Biotechnology,2006,25(6):111-114.(in Chinese)
[15]ZHOU Ping,LU Xiaoling,HU Chungang.Study on separation and purification of rosmarinic acid[J].Ion Exchange and Adsorption,2011,27(4):304-314.(in Chinese)
[16]BAI Duolong,YANG Kaihua.Macroporous adsorption resin purification technology and application[J].Strait Pharmaceutical Journal,2007,19(9):96-99.(in Chinese)
[17]SUN Xing,SHENG Huagang,ZHANG Chao,et al.Influence of chitosan flocculation sedimentation method on flavonoids and alkaloids from gegen Qinlian decoction[J].Chinese Journal of Experimental Traditional Medical Formulae,2012,18(20):12-15.(in Chinese)