HIF-1α在坏死股骨头中的基因表达
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摘要
目的通过实验观察股骨头局部缺氧诱导因子-1ɑ(hypoxia inducible factor-1ɑ,HIF-1ɑ)在正常与缺血股骨头坏死骨组织中的表达差异,探讨HIF-1ɑ在股骨头坏死中的发病相关机制。方法取正常股骨头、缺血坏死性股骨头骨组织各12例,样本组织2-4g用于HIF-1ɑ基因的RT-PCR。结果 HIF-1ɑ表达量在正常股骨头骨组织中与坏死组织相较更少,正常股骨头骨组织中RT-PCR的2~(-ΔΔCt)平均值为0.46±0.22,而在坏死股骨头骨组织中为0.55±0.24,两者比较差异存在统计学意义(P<0.05)。结论正常股骨头骨组织中的HIF-1ɑ基因与缺血坏死性股骨头骨组织具有统计学差异,故缺血缺氧导致股骨头骨组织中HIF-1ɑ基因表达量的增多可能为股骨头缺血坏死的重要原因。
Objective Through the experimental observation of femoral head local hypoxia inducing factor-1ɑ(hypoxia inducible factor-1ɑ,HIF-1ɑ) in normal and ischemic necrotic femoral head expression differences in bone tissue, to explore HIF-1ɑ in the pathogenesis of femoral head necrosis. Methods Taking each normal, ischemic necrotic femoral head bone 12 cases, femoral head sample group 2-4 g for HIF-1ɑ gene RT-PCR. Results HIF-1ɑ expression was less in normal bone tissue than in necrotic tissue, the mean value of RT-PCR 2~(-ΔΔCt) in normal bone tissue was 0.46±0.22, in necrotic bone tissue, it was 0.55±0.24, the difference was statistical significance between the both(P< 0.05).Conclusion HIF-1ɑ gene in normal and ischemic necrotic femoral head bone tissue was statistically significant, and as a result of ischemia hypoxia HIF-1ɑ gene expression increased quantity is probably one of the important reasons of the ischemic necrosis of the femoral head.
Objective Through the experimental observation of femoral head local hypoxia inducing factor-1ɑ(hypoxia inducible factor-1ɑ,HIF-1ɑ) in normal and ischemic necrotic femoral head expression differences in bone tissue, to explore HIF-1ɑ in the pathogenesis of femoral head necrosis. Methods Taking each normal, ischemic necrotic femoral head bone 12 cases, femoral head sample group 2-4 g for HIF-1ɑ gene RT-PCR. Results HIF-1ɑ expression was less in normal bone tissue than in necrotic tissue, the mean value of RT-PCR 2~(-ΔΔCt) in normal bone tissue was 0.46±0.22, in necrotic bone tissue, it was 0.55±0.24, the difference was statistical significance between the both(P< 0.05).Conclusion HIF-1ɑ gene in normal and ischemic necrotic femoral head bone tissue was statistically significant, and as a result of ischemia hypoxia HIF-1ɑ gene expression increased quantity is probably one of the important reasons of the ischemic necrosis of the femoral head.
引文
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[10]Zhang C,Shen M,Teng F,et al.Ultrasound-Enhanced Protective Effect of Tetramethylpyrazine via the ROS/HIF-1A Signaling Pathway in an in Vitro Cerebral Ischemia/Reperfusion Injury Model[J].Ultrasound Med Biol,2018,44(8):1786-1798.
[11]王络文,邓廉夫,朱雅萍,等.低氧/低氧诱导因子-1α通路对成骨细胞与破骨细胞耦联的调控作用[J].上海交通大学学报(医学版),2012,32(3):274-278,292.
[12]Samarpita S,Doss HM,Ganesan R,et al.Interleukin 17 under hypoxia mimetic condition augments osteoclast mediated bone erosion and expression of HIF-1αand MMP-9[J].Cell Immunol,2018(17):165.
[2]Alves EM,Angrisani AT,Santiago MB.The use of extracorporeal shoc K waves in the treatment of osteonecrosis of the femoral head:asystematic review[J].Clin Rheumatol,2009,28(11):1247-1251.
[3]Johannson HR,Zywiel MG,Mar Ker DR,et al.Osteonecrosis is not a predictor of poor outcomes in primary total hip arthroplasty:a systematic literature review[J].Int Orthop,2011,35(4):465-473.
[4]Lu X,Kang Y.Hypoxia and hypoxia-inducible factors:master regulators of metastasis[J].Clin Cancer Res,2010,16(24):5928-5935.
[5]徐涛涛,劳杨骏,廖菲,等.HIF-1a信号通路与激素性股骨头坏死相关性研究进展[J].世界中西医结合杂志,2015(5):730-733.
[6]李海军.股骨头坏死的病因与早期症状特点[J].世界最新医学信息文摘,2017,17(22):165.
[7]Knowles HJ,Athanasou NA.Hypoxia-inducible factor is expressed in giant cell tumour of bone and mediates paracrine effects of hypoxia on monocyteosteoclast differentiation via induction of VEGF[J].J Pathol,2008,215(1):56-66.
[8]Srinivasan S,Koenigstein A,Joseph J,et al.Role of mitochondrial reactive oxygen species in osteoclast differentiation[J].Ann N YAcad Sci,2010(1192):245-252.
[9]Utting JC,Flanagan AM,Brandao-Burch A,et al.Hypoxia stimulates osteoclast formation from human peripheral blood[J].Cell Biochem Funct,2010,28(5):374-380.
[10]Zhang C,Shen M,Teng F,et al.Ultrasound-Enhanced Protective Effect of Tetramethylpyrazine via the ROS/HIF-1A Signaling Pathway in an in Vitro Cerebral Ischemia/Reperfusion Injury Model[J].Ultrasound Med Biol,2018,44(8):1786-1798.
[11]王络文,邓廉夫,朱雅萍,等.低氧/低氧诱导因子-1α通路对成骨细胞与破骨细胞耦联的调控作用[J].上海交通大学学报(医学版),2012,32(3):274-278,292.
[12]Samarpita S,Doss HM,Ganesan R,et al.Interleukin 17 under hypoxia mimetic condition augments osteoclast mediated bone erosion and expression of HIF-1αand MMP-9[J].Cell Immunol,2018(17):165.