基于TOPSIS法对海南不同产地槟榔花的质量综合评价
|
摘要
[目的]系统研究槟榔花中的生物活性物质和体外抗氧化能力,为海南槟榔花的药用价值评价及产品开发提供理论依据。[方法]以70%乙醇超声提取并测定比较海南15种源产地槟榔雌花和雄花的总酚、总黄酮和总生物碱含量,采用DPPH自由基清除法、FRAP法和ABTS法测定提取液体外抗氧化能力,TOPSIS分析综合评价槟榔花药用价值。[结果]槟榔雄花的抗氧化能力普遍高于雌花,鸡心槟榔的抗氧化能力低于普通槟榔,乌石农场槟榔雄花WPM的抗氧化能力最强。[结论 ]TOPSIS法可作为多指标综合评价槟榔花质量的模型。
[Objective]The aim was to study systematically the bioactive substances and antioxidant capacity of Areca catechu flower,which would provide theoretical basis for the evaluation of medicinal value and product development of areca flower in Hainan. [Method] The ultrasonic extraction method with70% ethanol was used to determine the concentration of total phenols,total flavonoids and total alkaloids from female and male Areca catechu flowers from 15 different locations in Hainan. DPPH radical scavenging method,FRAP method and ABTS method was used to evaluate the antioxidant capacity.TOPSIS was used to evaluate the antioxidant capacity of Areca catechu flower. [Result] The antioxidant capacity of male areca catechu flower was generally higher than that of female Areca catechu flower. The antioxidant capacity of Jixin Areca catechu was lower than that of common. The antioxidant capacity of male Areca catechu flower in Wushi farm was the strongest.[Conclusion] TOPSIS method could be used as a model for quality evaluating of Areca catechu flower.
[Objective]The aim was to study systematically the bioactive substances and antioxidant capacity of Areca catechu flower,which would provide theoretical basis for the evaluation of medicinal value and product development of areca flower in Hainan. [Method] The ultrasonic extraction method with70% ethanol was used to determine the concentration of total phenols,total flavonoids and total alkaloids from female and male Areca catechu flowers from 15 different locations in Hainan. DPPH radical scavenging method,FRAP method and ABTS method was used to evaluate the antioxidant capacity.TOPSIS was used to evaluate the antioxidant capacity of Areca catechu flower. [Result] The antioxidant capacity of male areca catechu flower was generally higher than that of female Areca catechu flower. The antioxidant capacity of Jixin Areca catechu was lower than that of common. The antioxidant capacity of male Areca catechu flower in Wushi farm was the strongest.[Conclusion] TOPSIS method could be used as a model for quality evaluating of Areca catechu flower.
引文
[1]谭乐和.海南槟榔生产的现状、问题及对策[J].海南大学学报(自然科学版),2006,24(1):55-59.
[2]李小婷,庞玉新,杨全,等.四大南药资源开发利用现状分析[J].中国现代中药,2015,17(2):99-104.
[3]黄慧德.2015年槟榔产业发展报告及形势预测[J].世界热带农业信息,2017(01):31-39.
[4]符之学,刘立云,李艳,等.槟榔农业生产技术研究[J].安徽农业科学,2014,42(14):4229-4230,4292.
[5]谢龙莲,张慧坚,方佳.我国槟榔加工研究进展[J].广东农业科学,2011,38(4):96-98.
[6]刘月丽,徐汪伟,周丹,等.海南槟榔提取物抗衰老作用研究[J].中国热带医学,2017,17(2):123-125.
[7]晏小霞,王祝年,王建荣.槟榔种质资源研究概况[J].中国热带农业,2008(5):34-36.[8][宋]赵汝适.诸蕃志校释[M].杨博文校释.北京:中华书局,1996.
[9]XIAOJUN SHEN,WEIBO CHEN,YAJUN ZHENG,et al. Chemical composition,antibacterial and antioxidant activities of hydrosols from different parts of Areca catechu L. and Cocos nucifera L[J].Industrial Crops and Products,2017(96):110-119.
[10]张春梅.槟榔花提取物抗衰老作用研究[D].长沙:湖南农业大学,2011.
[11]战晴晴,周亚奎,刘洋洋,等.不同花期槟榔花槟榔碱的含量分析[J].精细化工,2013,30(3):285-287.
[12]FRAGA C G. Plant polyphenols:how to translate their in vitro antioxidantactionstoinvivoconditions[J].IubmbLife,2010,59(4-5):308-315.
[13]肖桂青,田云,卢向阳.荷叶总生物碱清除氧自由基作用的研究[J].化学与生物工程,2011,28(10):35-38.
[14]姜天甲,应铁进,陈秋平,等.油茶籽壳总黄酮的提取及抗氧化研究[J].中国食品学报,2010,10(1):93-99.
[15]叶洲辰,吴友根,于靖,等.油茶饼提取物的抗氧化活性研究及酚类化合物含量的测定[J].分子植物育种,2017,15(9):3682-3693.
[16]高帆,夏惠,王秀,等.不同甜樱桃品种抗氧化物质及抗氧化活性分析[J].浙江农业学报,2017,29(6):926-932.
[17]马文婷,吴友根,胡征波,等.诺丽内生真菌的分离鉴定及其次生代谢产物的抗氧化活性[J].热带生物学报,2017,8(4):424-430.
[18]田雪芬.槟榔青果不同方法提取物生物活性研究[D].开封:河南大学,2015.
[19]高敏,高淑芹.光度法测定槟榔中槟榔碱的含量[J].化学分析计量. 2003,12(1):28-30.
[20]田雪芬,李冬冬,康文艺,等.槟榔成方制剂治疗消化病的临床应用分析[J].中医研究,2016,29(1):78-80.
[21]RUPESH G,VIRUPANAGOUDA PP,SHIVAKUMAR H,et al.Antihyperglycemic activity of Areca Catechu flowers[J].Asian Pacific Journal of Tropical Disease,2014,4(1):S148-S152.
[22]AMOL MB,NEERAJ SV,AJAY D. Anti-migraine effect of Areca Catechu L. nut extract in bradykinin-induced plasma protein extravasation and vocalization in rats[J]. Journal of Ethnopharmacology,2015(171):121-124.
[23]LI-WEN HUANG,BAU-SHAN HSIEH,HSIAO-LING CHENG,et al. Arecoline decreases interleukin-6 production and induces apoptosis and cell cycle arrest in human basal cell carcinoma cells[J].Toxicology and Applied Pharmacology,2012,258(2):199-207.
[24]黄玉林,袁腊梅,兰淑惠,等.槟榔提取物抗菌活性的研究[J].食品科技,2009,34(1):202-204.
[25]FANGFANG CHENG,WEIJUN CHEN,YULIN HUANG,et al.Protective effect of areca inflorescence extract on hydrogen peroxideinduced oxidative damage to human serum albumin[J]. Food Research International,2011,44(1):98-102.
[26]YAN-FANG SUN,ZONG-SUO LIANG,CHANG-JUAN SHAN,et al. Comprehensive evaluation of natural antioxidants and antioxidant potentials in Ziziphus jujuba Mill. var. spinosa(Bunge)Hu ex H. F. Chou fruits based on geographical origin by TOPSIS method[J].Food Chemistry,2011,124(4):1612-1619.
[2]李小婷,庞玉新,杨全,等.四大南药资源开发利用现状分析[J].中国现代中药,2015,17(2):99-104.
[3]黄慧德.2015年槟榔产业发展报告及形势预测[J].世界热带农业信息,2017(01):31-39.
[4]符之学,刘立云,李艳,等.槟榔农业生产技术研究[J].安徽农业科学,2014,42(14):4229-4230,4292.
[5]谢龙莲,张慧坚,方佳.我国槟榔加工研究进展[J].广东农业科学,2011,38(4):96-98.
[6]刘月丽,徐汪伟,周丹,等.海南槟榔提取物抗衰老作用研究[J].中国热带医学,2017,17(2):123-125.
[7]晏小霞,王祝年,王建荣.槟榔种质资源研究概况[J].中国热带农业,2008(5):34-36.[8][宋]赵汝适.诸蕃志校释[M].杨博文校释.北京:中华书局,1996.
[9]XIAOJUN SHEN,WEIBO CHEN,YAJUN ZHENG,et al. Chemical composition,antibacterial and antioxidant activities of hydrosols from different parts of Areca catechu L. and Cocos nucifera L[J].Industrial Crops and Products,2017(96):110-119.
[10]张春梅.槟榔花提取物抗衰老作用研究[D].长沙:湖南农业大学,2011.
[11]战晴晴,周亚奎,刘洋洋,等.不同花期槟榔花槟榔碱的含量分析[J].精细化工,2013,30(3):285-287.
[12]FRAGA C G. Plant polyphenols:how to translate their in vitro antioxidantactionstoinvivoconditions[J].IubmbLife,2010,59(4-5):308-315.
[13]肖桂青,田云,卢向阳.荷叶总生物碱清除氧自由基作用的研究[J].化学与生物工程,2011,28(10):35-38.
[14]姜天甲,应铁进,陈秋平,等.油茶籽壳总黄酮的提取及抗氧化研究[J].中国食品学报,2010,10(1):93-99.
[15]叶洲辰,吴友根,于靖,等.油茶饼提取物的抗氧化活性研究及酚类化合物含量的测定[J].分子植物育种,2017,15(9):3682-3693.
[16]高帆,夏惠,王秀,等.不同甜樱桃品种抗氧化物质及抗氧化活性分析[J].浙江农业学报,2017,29(6):926-932.
[17]马文婷,吴友根,胡征波,等.诺丽内生真菌的分离鉴定及其次生代谢产物的抗氧化活性[J].热带生物学报,2017,8(4):424-430.
[18]田雪芬.槟榔青果不同方法提取物生物活性研究[D].开封:河南大学,2015.
[19]高敏,高淑芹.光度法测定槟榔中槟榔碱的含量[J].化学分析计量. 2003,12(1):28-30.
[20]田雪芬,李冬冬,康文艺,等.槟榔成方制剂治疗消化病的临床应用分析[J].中医研究,2016,29(1):78-80.
[21]RUPESH G,VIRUPANAGOUDA PP,SHIVAKUMAR H,et al.Antihyperglycemic activity of Areca Catechu flowers[J].Asian Pacific Journal of Tropical Disease,2014,4(1):S148-S152.
[22]AMOL MB,NEERAJ SV,AJAY D. Anti-migraine effect of Areca Catechu L. nut extract in bradykinin-induced plasma protein extravasation and vocalization in rats[J]. Journal of Ethnopharmacology,2015(171):121-124.
[23]LI-WEN HUANG,BAU-SHAN HSIEH,HSIAO-LING CHENG,et al. Arecoline decreases interleukin-6 production and induces apoptosis and cell cycle arrest in human basal cell carcinoma cells[J].Toxicology and Applied Pharmacology,2012,258(2):199-207.
[24]黄玉林,袁腊梅,兰淑惠,等.槟榔提取物抗菌活性的研究[J].食品科技,2009,34(1):202-204.
[25]FANGFANG CHENG,WEIJUN CHEN,YULIN HUANG,et al.Protective effect of areca inflorescence extract on hydrogen peroxideinduced oxidative damage to human serum albumin[J]. Food Research International,2011,44(1):98-102.
[26]YAN-FANG SUN,ZONG-SUO LIANG,CHANG-JUAN SHAN,et al. Comprehensive evaluation of natural antioxidants and antioxidant potentials in Ziziphus jujuba Mill. var. spinosa(Bunge)Hu ex H. F. Chou fruits based on geographical origin by TOPSIS method[J].Food Chemistry,2011,124(4):1612-1619.