白掌疫病病原菌的鉴定
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摘要
在致病性测定的基础上,通过形态特征观察及基于特异性引物Pn1和Pn2扩增的DNA序列分析,对在广州发现的白掌疫病的病原菌种类进行了鉴定。结果表明,引起广州白掌疫病的病原菌为烟草疫霉(Phytophthora nicotianae)。研究结果为白掌病害的防控提供了理论依据。
A strain was isolated from the diseased plants of Spathiphyllum sp. from Guangzhou. The Pathogenicity test showed the isolated strain was the pathogenic fugus of Spathiphyllum blight. The pathogenic strain was identified according to the morphology, rDNA internal transcribed spacer sequencing amplified by Pn1 和 Pn2. The result showed Phytophthora blight of Spathiphyllum from Guangzhou was caused by Phytophthora nicotianae. The result would provide a foundation for effective control of the disease.
A strain was isolated from the diseased plants of Spathiphyllum sp. from Guangzhou. The Pathogenicity test showed the isolated strain was the pathogenic fugus of Spathiphyllum blight. The pathogenic strain was identified according to the morphology, rDNA internal transcribed spacer sequencing amplified by Pn1 和 Pn2. The result showed Phytophthora blight of Spathiphyllum from Guangzhou was caused by Phytophthora nicotianae. The result would provide a foundation for effective control of the disease.
引文
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[13]Horinouchi H,Hagiwara H,Izutsu S,et al.Occurrence of Phytophthora rot of Spathiphyllum[J].Annals of The Phytopathological Society of Japan,1999,65(3):413-414.
[14]毕可可.匙叶天南星根茎腐烂病病原鉴定及生防菌株筛选[D].广州:华南农业大学,2009.
[15]马凯生.广州白掌主要土传真菌病害鉴定及防治药剂的筛选[D].广州:仲恺农业工程学院,2015.
[2]Uchida J Y.Foliar blight of Spathyphyllum caused by Phytophthora[Z].Honolulu(HI):University of Hawaii,1989.
[3]Uchida J Y.Pythium Root Rot of Spathiphyllum[Z].Honolulu(HI):University of Hawaii,1990.
[4]郑晓慧,戚佩坤,姜子德.广州地区天南星科观赏植物上的几种新真菌病害-II[J].华南农业大学学报,2001,22(2):39-41.
[5]方中达.植病研究法[M].北京:中国农业出版,1998.
[6]王洁菲.花椒疫霉菌(Phytophthora spp.)鉴定及其拮抗菌筛选[D].陕西:西北农林科技大学,2014.
[7]Chi M H,Park S Y,Lee Y H.A quick and safe method for fungal DNA extraction[J].The Plant Pathology Journal,2009,25(1):108-111.
[8]White T J,Bruns T S,Lee J T.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]//Ma Innis,Gelfand D H,Sninsky J J,et al.PCRProtocols:A Guide to Methods and Applications.New York:Academic Press,1990:315-322.
[9]高乔分,秦西云,方敦煌,等.烟草疫霉的快速分子检测[J].云南农业大学学报,2012,27(2):156-159.
[10]Alfieri Jr S A,Langdon K R,Wehlburg C,et al.Index of plant diseases in Florida(Revised)[Z].Florida:Department of Agriculture and Consumer Services,Division of Plant Industries,1984,11:1-389.
[11]陈永强,戚佩坤,姜子德.广州地区九种花卉疫病病原种的鉴定[J].华南农业大学学报,1998,20(4):5-9.
[12]郑晓慧.广州地区天南星科观赏植物真菌病害鉴定[D].广州:华南农业大学,1999.
[13]Horinouchi H,Hagiwara H,Izutsu S,et al.Occurrence of Phytophthora rot of Spathiphyllum[J].Annals of The Phytopathological Society of Japan,1999,65(3):413-414.
[14]毕可可.匙叶天南星根茎腐烂病病原鉴定及生防菌株筛选[D].广州:华南农业大学,2009.
[15]马凯生.广州白掌主要土传真菌病害鉴定及防治药剂的筛选[D].广州:仲恺农业工程学院,2015.