中药有效部位组方通痹四味方的体内抗炎、镇痛及活血通络等药效评价
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摘要
目的观察中药有效部位组方通痹四味方(TBSWF)体内抗炎镇痛、活血通络的药效,探究其作为风湿骨病候选组方的成药性。方法随机将昆明小鼠分为空白组,阿司匹林组(AP 250 mg·kg~(-1)),腰痛宁组(YTN220 mg·kg~(-1)),腰痹通组(YBT 560 mg·kg~(-1)),通痹四味方(TBSWF)高、中、低剂量组(50,100,200 mg·kg~(-1)),采用热致痛反应及醋酸致扭体反应模型考察组方的镇痛作用;采用二甲苯致耳廓肿胀模型考察组方的抗炎消肿作用;采用角叉菜胶诱发的足跖肿胀模型考察组方对急性关节炎的抑制效果;在以上7组的基础上再加入一组模型组,采用小鼠毛细血管通透性改变模型考察组方的活血通络作用。结果与空白组相比,二甲苯致耳廓肿胀模型TBSWF中剂量组和AP组肿胀率均显著降低(P<0.05,P<0.01);热致痛反应模型,TBSWF高剂量组在给药后0.5 h能够显著延长痛阈值(P<0.01);醋酸扭体实验中各组扭体次数均显著减少(P<0.01),其中TBSWF高、中剂量组的扭体次数均显著少于阿司匹林组及YTN、YBT组(P<0.01);角叉菜胶诱发的足跖肿胀实验中TBSWF方高、中剂量组分别在给药后1 h和2~3 h肿胀度显著降低(P<0.05,P<0.01)。小鼠毛细血管通透性实验中AP及TBSWF各剂量组的吸光度均低于模型组(P<0.01)。结论 TBSWF在抗炎、镇痛、活血通络及小鼠足肿胀急性关节炎模型下的表现均优于腰痛宁胶囊和腰痹通胶囊,并且各模型下都至少有一个TBSWF给药组与阿司匹林效果相当,TBSWF对小鼠醋酸扭体的镇痛效果显著优于3个阳性对照药。通痹四味方可作为治疗风湿骨病的候选中药复方。
Objective To explore the possibility of Tongbi Siwei Formula(TBSWF),a formula composed of traditional Chinese medicine effective parts,to be a candidate of traditional Chinese formula for curing rheumatoid bone diseases by observing its in-vivo anti- inflammatory,analgesic,and blood- activating and collateral- dredging effects. Methods Kunming mice were randomly divided into seven groups,namely blank control group,aspirin group(250 mg·kg-1),Yaotongning group(YTN,220 mg·kg~(-1)),Yaobitong group(YBT,560 mg·kg~(-1)),and low-,medium- and high- dose TBSWF groups(50, 100, 200 mg·kg-1). Hot- plate- induced pain response model and acetic- acid- induced writhing model were applied to investigate the analgesic effect, xylene- induced auricular edema model was used to study the anti- inflammatory effect, carrageenan- induced mice paw edema model was used to examine the effects on treating acute arthritis. By the establishment of a model group,mice capillary permeability test was adopted for the observation of blood- activating and collateral- dredging effects. Results Compared with the blank control group, aspirin and middle- dose TBSWF reduced the auricular swelling rate of xylene- induced ear edema animal model(P<0.01),and high- dose TBSWF increased the mice pain threshold 0.5 hour after medication in hot- plate- induced pain response test(P < 0.01); in acetic- acid- induced writhing experiment, the writhing frequency of all medicated groups was significantly reduced(P < 0.01),and the frequency in high- and middle- dose TBSWF groups was lower than that of aspirin group, YTN group and YBT group(P < 0.01); in carrageenan- induced paw edema experiment, swelling degree of high- and middle- dose TBSWF groups was reduced obviously one hour or 2- 3 hours after medication(P <0.01);in mice capillary permeability test,the absorbance of aspirin group and all TBSWF groups were significantly dropped(P < 0.01). Conclusion TBSWF has better anti- inflammatory, analgesic, blood- activating and collateraldredging,and anti- acute- arthritis effects than YTN and YBT,and TBSWF at certain dose in each experiment shows the effects similar to those of aspirin. Moreover,the analgesic effect of TBSWF on acetic- acid- induced writhing mice is significantly superior to that of the three positive control drugs. Therefore,it is feasible that TBSWF will be used as a candidate of traditional Chinese formula for curing rheumatoid bone diseases.
Objective To explore the possibility of Tongbi Siwei Formula(TBSWF),a formula composed of traditional Chinese medicine effective parts,to be a candidate of traditional Chinese formula for curing rheumatoid bone diseases by observing its in-vivo anti- inflammatory,analgesic,and blood- activating and collateral- dredging effects. Methods Kunming mice were randomly divided into seven groups,namely blank control group,aspirin group(250 mg·kg-1),Yaotongning group(YTN,220 mg·kg~(-1)),Yaobitong group(YBT,560 mg·kg~(-1)),and low-,medium- and high- dose TBSWF groups(50, 100, 200 mg·kg-1). Hot- plate- induced pain response model and acetic- acid- induced writhing model were applied to investigate the analgesic effect, xylene- induced auricular edema model was used to study the anti- inflammatory effect, carrageenan- induced mice paw edema model was used to examine the effects on treating acute arthritis. By the establishment of a model group,mice capillary permeability test was adopted for the observation of blood- activating and collateral- dredging effects. Results Compared with the blank control group, aspirin and middle- dose TBSWF reduced the auricular swelling rate of xylene- induced ear edema animal model(P<0.01),and high- dose TBSWF increased the mice pain threshold 0.5 hour after medication in hot- plate- induced pain response test(P < 0.01); in acetic- acid- induced writhing experiment, the writhing frequency of all medicated groups was significantly reduced(P < 0.01),and the frequency in high- and middle- dose TBSWF groups was lower than that of aspirin group, YTN group and YBT group(P < 0.01); in carrageenan- induced paw edema experiment, swelling degree of high- and middle- dose TBSWF groups was reduced obviously one hour or 2- 3 hours after medication(P <0.01);in mice capillary permeability test,the absorbance of aspirin group and all TBSWF groups were significantly dropped(P < 0.01). Conclusion TBSWF has better anti- inflammatory, analgesic, blood- activating and collateraldredging,and anti- acute- arthritis effects than YTN and YBT,and TBSWF at certain dose in each experiment shows the effects similar to those of aspirin. Moreover,the analgesic effect of TBSWF on acetic- acid- induced writhing mice is significantly superior to that of the three positive control drugs. Therefore,it is feasible that TBSWF will be used as a candidate of traditional Chinese formula for curing rheumatoid bone diseases.
引文
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[24]Su S,Hua Y,Wang Y,et al.Evaluation of the anti-inflammatory and analgesic properties of individual and combined extracts from Commiphora myrrha,and Boswellia carterii[J].Journal of Ethnopharmacology:An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs,2012,139(2):649-656.
[25]马艳苗,王永辉,李艳彦,等.风湿宁胶囊的主要药效学研究[J].世界中西医结合杂志,2012,07(9):806-809.
[26]滕忠,李茂,周军,等.济民风湿王抗炎、镇痛和对急性软组织损伤作用[J].中国实验方剂学杂志,2011,17(24):176-179.
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[28]吴文君,杜菲菲,刘全旭,等.复方儿茶止泻霜对大鼠足肿胀、小鼠耳肿胀及腹腔毛细血管通透性的影响[J].山东医药,2013,53(25):26-28.
[29]王本样.现代中药药理学[M].天津:天津科学技术出版社,1997:472.
[2]沈丕安.风湿病中医诊治手册[M].北京:人民军医出版社,2009.
[3]倪力军,张强祖,朱立中,等.腰痛宁胶囊治疗腰腿痛、腰肌劳损和风湿性关节炎临床研究的Meta分析[J].中成药,2012,34(9):1653-1660.
[4]张立国,欧阳霄雯,吴婷婷,等.腰痛宁胶囊药材活性部位不同组合对大鼠软骨细胞的影响[J].中国实验方剂学杂志,2014,20(10):151-155.
[5]Zhang LG,Ouyang XW,Wu TT,et al.Quantitative evaluation of in vitro effects and interactions of active fractions in a Chinese medicinal formula(Yaotongning Capsule)on rat chondrocytes[J].Journal of Ethnopharmacology,2014,155(3):1424-1432.
[6]倪力军,郭燕子,徐晓玲,等.腰痛宁胶囊及其拆方对Ana-1细胞免疫功能的影响[J].中成药,2014,36(11):2252-2257.
[7]Ni LJ,Xu XL,Zhang LG,Shi WZ.Quantitative evaluation of the in vitro effect and interactions of active fractions in Yaotongning-based formulae on prostaglandin E2production[J].Journal of Ethnopharmacology,2014,154(3):807-817.
[8]倪力军,徐晓玲,史万忠,等.腰痛宁胶囊活性部位组合对PGE2、IL-2、NO细胞因子的影响及其相互作用[J].中草药,2014,45(23):3424-3431.
[9]张立国,欧阳霄雯,郭燕子,等.腰痛宁胶囊处方药材有效部位及其组合对黄嘌呤代谢体外抑制活性的实验观察[J].中成药,2013,35(11):2342-2347.
[10]丁静,范丽萍.腰痛宁胶囊配合针灸治疗类风湿性关节炎的临床观察[J].河北医学,2012,18(8):1031-1033.
[11]党晓伟,宋跃进,郭金甲,等.腰痛宁胶囊治疗慢性风湿性关节炎(寒湿瘀阻证)多中心临床研究[J].中成药,2016,3(38):510-514.
[12]邢丽丽,高明利.腰痛宁胶囊治疗髋骨关节炎78例[J].风湿病与关节炎,2014,3(2):32-33.
[13]张立国,赵丽丽,倪力军,等.腰痛宁胶囊药材有效部位组合对IL-1β诱导大鼠滑膜细胞增殖及IL-6的影响[J].中国现代中药,2015,17(9):922-926.
[14]倪力军,朱婷婷,王南南,等.腰痛宁不同有效部位组方的细胞药理活性评价[J].世界科学技术-中药现代化,2015,17(5):963-970.
[15]倪力军,张立国,吴婷婷,等.用于治疗风湿骨病的中药组方及中成药的制备方法和应用[P].中国专利:201310540299.5,2014-2-19.
[16]欧阳霄雯.腰痛宁胶囊及其相关中药活性部位系列组方对大鼠关节炎细胞模型的分析与系统性评价[D].上海:华东理工大学,2014.
[17]郭燕子.以腰痛宁组方原则与活性部位为基础的系列中药复方对Ana-1巨噬细胞免疫活性的分析与系统评价[D].上海:华东理工大学,2014.
[18]徐晓玲.包括腰痛宁胶囊在内的不同中药活性部位组方的系统评价和分析[D].上海:华东理工大学,2014.
[19]倪力军,张立国,赵雯雯,等.治疗风湿骨病的中药组方及其中成药的制备方法[P].中国专利:201510095757.8,2015-07-01.
[20]马东升.通痹四味方中甘草皂苷的大鼠药代动力学及该组方的小鼠急性毒理学研究[D].上海:华东理工大学,2016.
[21]Gao LN,Cui YL,Wang QS,et al.Amelioration of Danhong injection on the lipopolysaccharide-stimulated systemic acute inflammatory reaction via multi-target strategy[J].Journal of Ethnopharmacology:An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs,2013,149(3):772-782.
[22]Gu S,Yin N,Pei J,et al.Understanding traditional Chinese medicine anti-inflammatory herbal formulae by simulating their regulatory functions in the human arachidonic acid metabolic network[J].Molecular bio Systems,2013,9(7):1931-1938.
[23]Su JY,Tan LR,Lai P,et al.Experimental study on antiinflammatory activity of a TCM recipe consisting of the supercritical fluid CO2extract of Chrysanthemum indicum,Patchouli Oil and Zedoary Turmeric Oil in vivo[J].Journal of Ethnopharmacology:An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs,2012,141(2):608-614.
[24]Su S,Hua Y,Wang Y,et al.Evaluation of the anti-inflammatory and analgesic properties of individual and combined extracts from Commiphora myrrha,and Boswellia carterii[J].Journal of Ethnopharmacology:An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs,2012,139(2):649-656.
[25]马艳苗,王永辉,李艳彦,等.风湿宁胶囊的主要药效学研究[J].世界中西医结合杂志,2012,07(9):806-809.
[26]滕忠,李茂,周军,等.济民风湿王抗炎、镇痛和对急性软组织损伤作用[J].中国实验方剂学杂志,2011,17(24):176-179.
[27]王利彦,车胜荣,周军兰,等.五味祛风湿消痛灵抗炎、镇痛作用的实验研究[J].第三军医大学学报,2003,25(17):1504-1504,1510.
[28]吴文君,杜菲菲,刘全旭,等.复方儿茶止泻霜对大鼠足肿胀、小鼠耳肿胀及腹腔毛细血管通透性的影响[J].山东医药,2013,53(25):26-28.
[29]王本样.现代中药药理学[M].天津:天津科学技术出版社,1997:472.