朱红膏中汞在大鼠皮肤不同损伤状态下的体外透过速率比较研究
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摘要
目的比较朱红膏中的汞在不同损伤程度的大鼠皮肤中的体外透过速率的差异,了解皮肤损伤状态对朱红膏中有毒成分汞透皮吸收的影响。方法结合皮肤的组织病理学检查,对皮肤的损伤程度进行判断,同时采用透皮扩散仪进行透皮实验,运用原子荧光法检测接收液中的汞含量。结果通过不同的处理方式得到不同损伤程度的皮肤,正常组皮肤完好,砂纸打磨使皮肤角质层受到破坏,脱毛剂使皮肤真皮浅层缺失。正常组汞的透过速率明显小于其他两组,砂纸组和脱毛剂组的汞的透过速率基本相同,汞的累积透过量没有显著差异。结论皮肤角质层是限制朱红膏中汞透过皮肤的主要障碍,朱红膏中汞的吸收量不会因为皮肤损伤程度的不同而产生较大差异。
Objective To research the effects of transdermal absorption about the mercury in Zhuhong ointment on the damaged skin,and compare the penetration rate in vitro of damaged skin prepared by different ways on rats. Methods The injured degree of damaged skin was evaluate by histopathological examination. Transdermal diffusion apparatus was used to carry out skin penetration test and atomic fluorescence spectrometry was used to detect mercury content in the receiver. Results The damaged skins of different levels were dealt by different ways. The histopathological results indicated that the structure of normal skins were intact,and the keratoderma of skins were destroyed by dealing with abrasive paper,and the corium of skins were missing by dealing with depilatory. The penetration rate of mercury on the normal skin was significantly lower than on the other groups,but the penetration rates of the other two groups were basically same. Conclusion The stratum corneum was the main obstacle to limiting the permeability of mercury in Zhuhong ointment. The amount of mercury in the Zhuhong ointment does not vary greatly because of the degree of skin damage.
Objective To research the effects of transdermal absorption about the mercury in Zhuhong ointment on the damaged skin,and compare the penetration rate in vitro of damaged skin prepared by different ways on rats. Methods The injured degree of damaged skin was evaluate by histopathological examination. Transdermal diffusion apparatus was used to carry out skin penetration test and atomic fluorescence spectrometry was used to detect mercury content in the receiver. Results The damaged skins of different levels were dealt by different ways. The histopathological results indicated that the structure of normal skins were intact,and the keratoderma of skins were destroyed by dealing with abrasive paper,and the corium of skins were missing by dealing with depilatory. The penetration rate of mercury on the normal skin was significantly lower than on the other groups,but the penetration rates of the other two groups were basically same. Conclusion The stratum corneum was the main obstacle to limiting the permeability of mercury in Zhuhong ointment. The amount of mercury in the Zhuhong ointment does not vary greatly because of the degree of skin damage.
引文
[1]吕培文,苍张,宋孝瑜,等.朱红膏治疗慢性溃疡的临床研究[J].中国中西医结合外科杂志,2003,9(5):17-19.
[2]董建勋,罗玲,李建荣,等.朱红膏促进皮肤溃疡愈合的安全性研究[J].中国中医药信息杂志,2011,18(6):47-49.
[3]梁爱华,商敏凤.朱砂的毒性研究概况[J].中国中药杂志,2005,30(4):249-252.
[4]薛建国,夏春丽,宋艺君.朱砂的功效及毒性研究进展[J].现代中医药,2014,(2):66-69.
[5]梁爱华,王金华,薛宝云,等.朱砂对大鼠的肝肾毒性研究[J].中国中药杂志,2009,34(3):312-318.
[6]孙新民,王旗,牟稷征,等.玉红膏单次给药家兔体内汞的吸收及毒性[J].中国实验方剂学杂志,2011,17(24):193-196.
[7]Huang C,Liu S,Linshiau S.Neurotoxicological effects of cinnabar(a Chinese mineral medicine,Hg S)in mice[J].Toxicology and Applied Pharmacology,2007,224(2):192-201.
[8]Wang Y,Wang D,Wu J,et al.Cinnabar-Induced Subchronic Renal Injury Is Associated with Increased Apoptosis in Rats[J].Bio Med Research International,2015,(3):1-10.
[9]Syversen T,Kaur P.The toxicology of mercury and its compounds[J].Journal of Trace Elements in Medicine and Biology,2012,26(4):215-226.
[10]Clarkson T W,Magos L.The toxicology of mercury and its chemical Compounds[J].Crit Rev Toxicol,2006,36(8):609.
[11]林含,张旭辉,董建勋,等.朱红膏对破损皮肤模型大鼠汞蓄积及肾组织形态的影响[J].中国中药杂志,2012,37(6):739-743.
[12]王乐平,罗玲,董建勋,等.中药外用制剂朱红膏对皮肤溃疡模型大鼠肾脏过氧化及金属硫蛋白的影响[J].中国中医药信息杂志,2011,18(12):47-49.
[13]王乐平.朱红膏肾毒性及机制研究[D].北京:北京中医药大学,2011.
[14]田璐,魏金锋,王爱平.经皮给药实验应注意的问题[C]//2013年(第三界)中国药物毒理学年会暨药物非临床安全性评价研究论坛,2013.
[2]董建勋,罗玲,李建荣,等.朱红膏促进皮肤溃疡愈合的安全性研究[J].中国中医药信息杂志,2011,18(6):47-49.
[3]梁爱华,商敏凤.朱砂的毒性研究概况[J].中国中药杂志,2005,30(4):249-252.
[4]薛建国,夏春丽,宋艺君.朱砂的功效及毒性研究进展[J].现代中医药,2014,(2):66-69.
[5]梁爱华,王金华,薛宝云,等.朱砂对大鼠的肝肾毒性研究[J].中国中药杂志,2009,34(3):312-318.
[6]孙新民,王旗,牟稷征,等.玉红膏单次给药家兔体内汞的吸收及毒性[J].中国实验方剂学杂志,2011,17(24):193-196.
[7]Huang C,Liu S,Linshiau S.Neurotoxicological effects of cinnabar(a Chinese mineral medicine,Hg S)in mice[J].Toxicology and Applied Pharmacology,2007,224(2):192-201.
[8]Wang Y,Wang D,Wu J,et al.Cinnabar-Induced Subchronic Renal Injury Is Associated with Increased Apoptosis in Rats[J].Bio Med Research International,2015,(3):1-10.
[9]Syversen T,Kaur P.The toxicology of mercury and its compounds[J].Journal of Trace Elements in Medicine and Biology,2012,26(4):215-226.
[10]Clarkson T W,Magos L.The toxicology of mercury and its chemical Compounds[J].Crit Rev Toxicol,2006,36(8):609.
[11]林含,张旭辉,董建勋,等.朱红膏对破损皮肤模型大鼠汞蓄积及肾组织形态的影响[J].中国中药杂志,2012,37(6):739-743.
[12]王乐平,罗玲,董建勋,等.中药外用制剂朱红膏对皮肤溃疡模型大鼠肾脏过氧化及金属硫蛋白的影响[J].中国中医药信息杂志,2011,18(12):47-49.
[13]王乐平.朱红膏肾毒性及机制研究[D].北京:北京中医药大学,2011.
[14]田璐,魏金锋,王爱平.经皮给药实验应注意的问题[C]//2013年(第三界)中国药物毒理学年会暨药物非临床安全性评价研究论坛,2013.