辅酶Q10的分离纯化及生物活性的研究进展
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摘要
近年来,国内外许多专家学者在辅酶Q10的分离、纯化及生物活性等方面进行了大量研究,并取得了较大进展。本文综述了辅酶Q10的3种制备方法:动植物组织提取法,微生物发酵法和化学合成法;分离纯化常用的3种方法以及辅酶Q10的生物活性,主要表现在抗氧化、抗肿瘤、抗心脑血管疾病、增强免疫力等方面,这些生物活性使其在食品、医药、美容等领域拥有广阔的应用前景。
Coenzyme Q10(CoQ10)is a kind of anthraquinones,which gets so much attentions all around the world.In this paper,the extraction purification and biological activity of CoQ10were reviewed.There were three methods for the preparation of CoQ10,mainly including propagation cell extraction,chemical synthesis and microbiological fermentation.There were three methods of separation and purification such as chromatography.The biological activity of CoQ10displays on anti-oxidation,antineoplastic,cancer and heart failure treatment,immunomodulation.CoQ10has broad application prospect in food,medicine,cosmetology and so on.
Coenzyme Q10(CoQ10)is a kind of anthraquinones,which gets so much attentions all around the world.In this paper,the extraction purification and biological activity of CoQ10were reviewed.There were three methods for the preparation of CoQ10,mainly including propagation cell extraction,chemical synthesis and microbiological fermentation.There were three methods of separation and purification such as chromatography.The biological activity of CoQ10displays on anti-oxidation,antineoplastic,cancer and heart failure treatment,immunomodulation.CoQ10has broad application prospect in food,medicine,cosmetology and so on.
引文
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[29]陈星,刘蕾,吴琼.分子蒸馏法从大豆油脚中提取辅酶Q10的研究[J].中国油脂,2011,36(10):73-76
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[32]何炳林,黄文强.离子交换与吸附树脂[M].上海:上海科技教育出版社.1992
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[36]曹学丽,徐亚涛,章光明,等.高速逆流色谱法分离纯化微生物发酵产辅酶Q10[A].中国香料香精化妆品工业协会:2006:6
[37]Bentinger M,Tekle M,Dallner G.Coenzyme Q——biosynthesis and functions[J].Biochemical and biophysical research communications,2010,396(1):74-79
[38]Abdin AA,Hamouda HE.Mechanism of the neuroprotective role of coenzyme Q10with or without L-dopa inrotenone-induced parkinsonism[J].Neuropharmacology,2009,55(8),1340-1346
[39]Ahmadvand H,Tavafi M,Khosrowbeygi A.Amelioration of altered antioxidant enzymes activity and glomerulosclerosis by coenzyme Q10in alloxan-induced diabetic rats[J].Journal of Diabetes and its Complications,2012,26(6):476-482
[40]Korotdova EI,Avramchik OA,Kagiya TV.Study of antioxidant properties of water-soluble Vitamin E derivate–tocopherol monoglucoside(TMG)by differential pulse voltammetry[J].Talanta,2004,63:729-734
[41]Polak T,ˇZlender B.Effects of coenzyme Q10,α-tocopherol and ascorbic acid on oxidation of cholesterol in chicken liver paté[J].LWT-Food Science and Technology,2011,44(4):1052-1058
[42]吴祖芳,翁佩芳,陈坚.辅酶Q10的功能研究进展[J].宁波大学学报,2001,14(2):85-88
[43]Portakal O,Erdeninal M.Coenzyme Q10concentractions and antioxidant status in tissues of breast cancer patients[J].Clinical Biochemistry,2000,36(4):279-284.
[44]Fouad AA,Al-Mulhim AS,Jresat I.Therapeutic effect of coenzyme Q10against experimentally-induced hepatocellular carcinoma in rats[J].Environmental Toxicology and Pharmacology,2013,35(1):100-108
[45]Singh R,Niaz M.Serum concentration of lipoprotein(a)decreases on treatment with hydrosoluble coenzyme Q10in patients with coronary artery disease:discovery of a new role[J].International Journal of Cardiology,1999,69(1):23-29
[46]Tiano L,Belardinelli R.Effect of coenzyme Q10administration on endothelial function and extracellular superoxide dismutase in patients with ischaemic heart disease:a double-blind,randomized controlled study[J].Eur Heart J,2007,28(18):2249-2255
[47]Toyama K,Sugiyama S.Rosuvastatin combined with regular exercise preserves coenzyme Q10levels associated with a significant increase in high-density lipoprotein cholesterol in patients with coronary artery disease[J].Atherosclerosis,2011,217(1):158-164
[48]赵春玉,赵宝东,王雅君,等.辅酶Q10在帕金森病中的应用[J].中华神经科杂志,2003,36(4):314
[49]Sharma SK,Hesham ER,Manuchair E.Complex-1activity and 18F-DOPA uptake in genetically engineered mouse model of Parkinson’s disease and the neuroprotective role of coenzyme Q10[J].Brain Research Bulletin,2006,70:22-32
[50]Mischley LK,Allen J,Bradley R.Coenzyme Q10deficiency in patients with Parkinson's disease[J].Journal of the Neurological Sciences,2012,318(1–2):72-75
[51]Rusciani L,Proietti I,Rusciani A.Low plasma coenzyme Q10levels as an independent prognostic factor for melnoma progression[J].Journal of the American Academy of Dermatology,2006,54(2):234-235
[52]洪钢,方红.天然抗氧化剂维生素E、维生素C、辅酶Q10的光保护作用[J].国外医学皮肤性病学分册,2003,29(4):241-242
[53]李琼.辅酶Q10的作用及在日化用品中的应用[J].口腔护理用品工业,2012,22(2):37-39
[54]许海燕.辅酶Q10研究进展[J].广州化工,2013,41(5):64-65
[2]Yu ting TIAN,Tianli YUE,Yahong YUAN.Improvement of cultivation medium for enhanced production of coenzyme Q10by photosynthetic Rhodospirillum rubrum[J].Biochemical Engineering Journal,2010,51:160-166
[3]Zatar NA,Abu-Eid MA,EidAF.Spectrophotometric determination of nitrite and nitrate using phosphomolybdenum blue complex[J].Ta-lanta,1999,(50):819-826
[4]Eren D,Keinan E.Total synthesis of linear polyprenoids.3.Synthesis of ubiquinones via palladium-catalyed oligomerlzation of monoterpene monomers[J].J Am Chem Soc,1988,110(13):4356-4362
[5]张双奇,昝林森,田万强,等.牛心肌中辅酶Q10的提取及测定[J].中国农学通报,2007,23(4):16-17
[6]王改玲,宋瑞雯,陶志杰,等.花生中辅酶Q10的提取工艺及含量测定[J].食品与发酵工业,2012,05:236-239
[7]陶志杰,曹迪,王改玲,等.超声波法辅助提取花生中辅酶Q10的工艺优化[J].食品科学,2012,33(18):53-56
[8]李春英,赵春建,祖元刚,等.超临界二氧化碳同时分离烟草提取物中的茄尼醇和辅酶Q10[J].林产化学与工业,2010,30(6):1-6
[9]杨立军.辅酶Q10的现代研究进展[J].海峡药学,2008,20(12):135-137
[10]黄伟,徐建忠,冯晓亮,等.辅酶Q10研究新进展[J].河南化工,2003,(2):12-14
[11]杨红梅.辅酶Q10生物学效应与合成[J].江西化工,2010,(3):1-5
[12]郜晓峰,罗应刚,官家发,等.微生物发酵法生产辅酶Q10研究进展[J].天然产物研究与开发,2006,18(5):858-862,872
[13]陶志杰,王改玲,李妍,等.辅酶Q10的制备及应用新进展[J].畜牧与饲料科学,2010,31(9):9-11
[14]李家洲,张冬青,肖玉平,等.辅酶Q10生物合成与生产研究进展[J].生物技术通报,2011,10:37-42
[15]潘仙华,毛海舫,吴晓.泛醌(辅酶Q10)的合成研究[J].香料香精化妆品,2006,05:1-4
[16]杨运泉,王威燕,童刚生,等.半化学法合成辅酶Q10[J].湘潭大学自然科学学报,2008,30(3):94-97
[17]陈玥.辅酶Q10创新合成工艺研究[D].东北林业大学,2010
[18]庄秀红,陈林,杨章萍,等.辅酶Q10菌种选育及高通量筛选研究进展[J].农产品加工·学刊,2012,(3):46-50
[19]郑毅,王娅,朱志春等.发酵法生产辅酶Q10研究进展[J].海峡科学,2012,08:128-130
[20]潘春梅,堵国成,陈坚.辅酶Q(10)高产菌Rhizobium radiobacter的选育及发酵条件优化[J].过程工程学报,2004,05:451-456
[21]王飞飞.原生质体融合构建辅酶Q10高产菌株的研究[D].陕西:西北农林科技大学,2008
[22]党磊,田菁,印红,等.利用空间诱变选育辅酶Q(10)高产菌[J].科技导报,2010,14:40-43
[23]宋丽雅,乔志新,李伟静,等.利用基因组改组技术提高辅酶Q10产量[J].科技导报,2012,24:66-70
[24]王冀民,马捷,仓基勇,等.辅酶Q10的微生物发酵工艺研究[J].河北化工,2009,32(8):47-48
[25]Hossein SZ,Sang HY,Jay DK.Coenzyme Q 10production in recombinant Escherichia coli strains engineered with a heterologous decaprenyl diphosphate synthase gene and foreign mevalonate pathway[J].Metab Eng,2006,8(5):406-416
[26]赵树全,刘悦才,江国托.酿酒酵母菌体中辅酶Q10的提取及测定方法[J].酿酒科技,2008(11):114-116
[27]Yoshida H,Kotani Y,Ochiai K,et al.Production of ubiquinone-10using bacteria[J].The Journal of general and applied microbiology,1998,44(1):19-26
[28]王春林.中国大豆辅酶Q10的提取、分离和鉴定[J].中国医药工业杂志,1996,03:102-104
[29]陈星,刘蕾,吴琼.分子蒸馏法从大豆油脚中提取辅酶Q10的研究[J].中国油脂,2011,36(10):73-76
[30]洪晨江,徐环昕,刘坐镇,等.辅酶Q10的纯化[J].中国医药工业杂志,2011,42(12):894-898
[31]陶志杰,王改玲,李妍,等.柱层析法分离纯化辅酶Q(10)研究[J].蚌埠学院学报,2012,03:11-14
[32]何炳林,黄文强.离子交换与吸附树脂[M].上海:上海科技教育出版社.1992
[33]李燕超,朱维忠,常小菲.大孔树脂吸附法分离纯化CoQ(10)的研究[J].科技资讯,2007,15:2
[34]洪晨江,徐环昕,刘坐镇,等.辅酶Q(10)的纯化[J].中国医药工业杂志,2011,12:894-898
[35]国家药典委员会编.中华人民共和国药典2010年版(二部)[M].北京:中国医药科技出版社,2010:882-883
[36]曹学丽,徐亚涛,章光明,等.高速逆流色谱法分离纯化微生物发酵产辅酶Q10[A].中国香料香精化妆品工业协会:2006:6
[37]Bentinger M,Tekle M,Dallner G.Coenzyme Q——biosynthesis and functions[J].Biochemical and biophysical research communications,2010,396(1):74-79
[38]Abdin AA,Hamouda HE.Mechanism of the neuroprotective role of coenzyme Q10with or without L-dopa inrotenone-induced parkinsonism[J].Neuropharmacology,2009,55(8),1340-1346
[39]Ahmadvand H,Tavafi M,Khosrowbeygi A.Amelioration of altered antioxidant enzymes activity and glomerulosclerosis by coenzyme Q10in alloxan-induced diabetic rats[J].Journal of Diabetes and its Complications,2012,26(6):476-482
[40]Korotdova EI,Avramchik OA,Kagiya TV.Study of antioxidant properties of water-soluble Vitamin E derivate–tocopherol monoglucoside(TMG)by differential pulse voltammetry[J].Talanta,2004,63:729-734
[41]Polak T,ˇZlender B.Effects of coenzyme Q10,α-tocopherol and ascorbic acid on oxidation of cholesterol in chicken liver paté[J].LWT-Food Science and Technology,2011,44(4):1052-1058
[42]吴祖芳,翁佩芳,陈坚.辅酶Q10的功能研究进展[J].宁波大学学报,2001,14(2):85-88
[43]Portakal O,Erdeninal M.Coenzyme Q10concentractions and antioxidant status in tissues of breast cancer patients[J].Clinical Biochemistry,2000,36(4):279-284.
[44]Fouad AA,Al-Mulhim AS,Jresat I.Therapeutic effect of coenzyme Q10against experimentally-induced hepatocellular carcinoma in rats[J].Environmental Toxicology and Pharmacology,2013,35(1):100-108
[45]Singh R,Niaz M.Serum concentration of lipoprotein(a)decreases on treatment with hydrosoluble coenzyme Q10in patients with coronary artery disease:discovery of a new role[J].International Journal of Cardiology,1999,69(1):23-29
[46]Tiano L,Belardinelli R.Effect of coenzyme Q10administration on endothelial function and extracellular superoxide dismutase in patients with ischaemic heart disease:a double-blind,randomized controlled study[J].Eur Heart J,2007,28(18):2249-2255
[47]Toyama K,Sugiyama S.Rosuvastatin combined with regular exercise preserves coenzyme Q10levels associated with a significant increase in high-density lipoprotein cholesterol in patients with coronary artery disease[J].Atherosclerosis,2011,217(1):158-164
[48]赵春玉,赵宝东,王雅君,等.辅酶Q10在帕金森病中的应用[J].中华神经科杂志,2003,36(4):314
[49]Sharma SK,Hesham ER,Manuchair E.Complex-1activity and 18F-DOPA uptake in genetically engineered mouse model of Parkinson’s disease and the neuroprotective role of coenzyme Q10[J].Brain Research Bulletin,2006,70:22-32
[50]Mischley LK,Allen J,Bradley R.Coenzyme Q10deficiency in patients with Parkinson's disease[J].Journal of the Neurological Sciences,2012,318(1–2):72-75
[51]Rusciani L,Proietti I,Rusciani A.Low plasma coenzyme Q10levels as an independent prognostic factor for melnoma progression[J].Journal of the American Academy of Dermatology,2006,54(2):234-235
[52]洪钢,方红.天然抗氧化剂维生素E、维生素C、辅酶Q10的光保护作用[J].国外医学皮肤性病学分册,2003,29(4):241-242
[53]李琼.辅酶Q10的作用及在日化用品中的应用[J].口腔护理用品工业,2012,22(2):37-39
[54]许海燕.辅酶Q10研究进展[J].广州化工,2013,41(5):64-65