摘要
在弱碱性乙醇溶液中,溴甲酚绿与盐酸米多君以静电引力结合生成具有1个较强正吸收峰和1个较强负吸收峰的二元离子缔合物,最大正吸收峰位于553 nm,最大负吸收峰位于444 nm,表观摩尔吸光系数(κ)分别为2. 06×104L/(mol·cm)(553 nm)和2. 07×104L/(mol·cm)(444 nm)。当用双波长法测定时,表观摩尔吸光系数(κ)可达4. 13×104L/(mol·cm),线性范围均为0. 2~8. 7 mg/L,检出限分别为0. 15 mg/L(单波长法,553 nm)、0. 14 mg/L(单波长法,444 nm)和0. 072 mg/L(双波长法)。据此建立了测定盐酸米多君的双波长可见吸收光谱法,该方法用于盐酸米多君片剂中盐酸米多君的测定,回收率和相对标准偏差RSD(n=5)分别为98. 4%~102%和1. 8%~2. 3%。
In a weak-alkaline ethanol solution,bromocresol green combines with midodrine hydrochloride by electrostatic attraction to form a binary ionic association complex with a strong positive absorption peak and a strong negative absorption peak.The maximum positive absorption peak locates at 553 nm and the minimum negative absorption peak locates at 444 nm.The apparent molar absorptivity(κ) are 2. 06×104 L/(mol·cm) for 553 nm peak and 2. 07×104 L/(mol·cm) for 444 nm peak,respectively.When the dual-wavelength absorption spectrophotometry is used to determine the content of midodrine hydrochloride,its apparent molar absorptivity(κ) can achieve 4. 13× 104 L/(mol·cm),their linear ranges both are in the range of 0. 2 to 8. 7 mg·L-1,and their detection limits are 0. 072 mg·L-1.When the singlewavelength method is used,the detection limits are 0. 15 mg·L-1(for 553 nm) and 0. 14 mg·L-1(for 444 nm).Accordingly,a dual-wavelength visible absorption spectrometric method for the determination of midodrine hydrochloride is established.The recovery rate and relative standard deviation RSD(n = 5) are 98. 4%-102% and 1. 8%-2. 3%,respectively when the method is applied to determine the content of midodrine hydrochloride in commercially available midodrine hydrochloride tablets.
引文
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