牦牛隐性乳房炎的调查及致病菌的分离鉴定
|
摘要
为调查阿坝州红原县牦牛隐性乳房炎的发病情况,无菌采取68份无临床乳房炎牦牛奶样,采用体细胞计数、细菌分离培养、革兰染色与PCR相结合的方法分离鉴定细菌种属,并统计分析体细胞数与细菌分离情况的相关性。结果显示,牦牛奶样体细胞数从4 000个/mL～240 000个/mL不等;各种细菌阳性奶样数分别为金黄色葡萄球菌1份,溶血性葡萄球菌30份,非溶血性葡萄球17份,大肠埃希菌2份,乳酸乳球菌18份和藤黄微球菌2份;未分离到无乳、停乳、乳房链球菌。结果表明,分离到溶血性葡萄球菌的牦牛奶样体细胞平均数显著高于未分离到溶血性葡萄球菌的牦牛奶样体细胞的平均数(P<0.05),当牦牛奶中体细胞数大于90 000个/mL时,溶血性葡萄球菌的阳性率大于80%(10/12)。研究结果提示溶血性葡萄球菌是引起牦牛隐性乳房炎的病原菌,为牦牛隐性乳房炎的防控提供了依据。
In order to investigate yak subclinical mastitis in Hongyuan county,Aba district,and provide a scientific basis for the prevention and control of yak subclinical mastitis.In this test,68 yak milk samples without clinical mastitis taken aseptically were used to detect the number of somatic cells,the isolated bacteria from the same samples were identified by Gram staining and PCR,the correlation between somatic cell count and bacterial isolation results was statistically analyzed.The results showed that the number of somatic cells ranged from 4 000/mL to 240 000/mL,1 case of Staphylococcus aureus positive milk,30 cases of hemolytic staphylococci,17 cases of non-hemolytic staphylococci,2 cases of E.coli,18 cases of Lactococcus lactis and 2 cases of M.luteus were identified,and there were no Streptococcus dysgalactiae,Streptococcus agalactiae,and Streptococcus uberis isolated.The results showed that the hemolytic Staphylococcus was isolated significantly higher from the milk samples with higher average number of somatic cells than those of lower ones(P<0.05).When the number of somatic cells in yak milk was greater than 90 000/mL,the positive rate of hemolytic staphylococci is greater than 80%(10/12).Therefore,it is speculated that the important pathogens of yak subclinical mastitis are hemolytic staphylococci.This study found that hemolytic Staphylococcus was the important pathogen causing yak subclinical mastitis,which provided a scientific basis for the prevention and control of yak subclinical mastitis.
In order to investigate yak subclinical mastitis in Hongyuan county,Aba district,and provide a scientific basis for the prevention and control of yak subclinical mastitis.In this test,68 yak milk samples without clinical mastitis taken aseptically were used to detect the number of somatic cells,the isolated bacteria from the same samples were identified by Gram staining and PCR,the correlation between somatic cell count and bacterial isolation results was statistically analyzed.The results showed that the number of somatic cells ranged from 4 000/mL to 240 000/mL,1 case of Staphylococcus aureus positive milk,30 cases of hemolytic staphylococci,17 cases of non-hemolytic staphylococci,2 cases of E.coli,18 cases of Lactococcus lactis and 2 cases of M.luteus were identified,and there were no Streptococcus dysgalactiae,Streptococcus agalactiae,and Streptococcus uberis isolated.The results showed that the hemolytic Staphylococcus was isolated significantly higher from the milk samples with higher average number of somatic cells than those of lower ones(P<0.05).When the number of somatic cells in yak milk was greater than 90 000/mL,the positive rate of hemolytic staphylococci is greater than 80%(10/12).Therefore,it is speculated that the important pathogens of yak subclinical mastitis are hemolytic staphylococci.This study found that hemolytic Staphylococcus was the important pathogen causing yak subclinical mastitis,which provided a scientific basis for the prevention and control of yak subclinical mastitis.
引文
[1]Leitner G,Zilberman D,Papirov E,et al.Assessment of acoustic pulse therapy(APT),anon-antibiotic treatment for dairy cows with clinical and subclinical mastitis[J].PLoS One,2018,13(7):e0199195.
[2]车车.无乳链球菌、停乳链球菌、乳房链球菌GapC蛋白的表达与免疫原性研究[D].黑龙江大庆:黑龙江八一农业大学,2009:1-2.
[3]Preethirani P L,Isloor S,Sundareshan S.Isolation,biochemical and molecular identification,and in vitro antimicrobial resistance patterns of bacteria isolated from bubaline subclinical mastitis in South India[J].PLoS One,2015,10(11):e0142717.
[4]田会丽,袁筠,廖超.石河子地区乳源性金黄色葡萄球菌毒力及耐药性的研究[J].现代食品科技,2017(9):266.
[5]潘玉琨,谢珊珊,党斌,等.山羊临床型乳房炎主要病原菌分离鉴定及药敏试验[J].动物医学进展,2015,36(7):122-125.
[6]周义正,李向阳,邱小燕,等.PCR法快速检测阳性血培养瓶中葡萄球菌属[J].中华医院感染学杂志,2008,18(12):1798-1800.
[7]夏雪娟,郑炯,叶秀娟,等.实时荧光定量PCR技术监测腌制麻竹笋中乳酸乳球菌动态变化[J].食品科学,2016,37(4):88-92.
[8]胡翀,马薇,张可昕,等.多重PCR方法快速检测血流感染10种常见病原菌方法的建立与应用[J].中华医院感染学杂志,2018(7).
[9]胡勇.牦牛乳制品加工进展[J].青海畜牧兽医杂志,2009,39(1):44-45.
[10]刘诗吟.国际化发展的新机遇---2017中国牦牛乳产业发展论坛侧记[J].甘肃农业,2017(15):8-12.
[11]孙西峰,沈延银.牦牛隐性乳房炎发病情况的调查[J].青海畜牧兽医杂志,2009,39(3):10.
[12]白龙.呼市地区奶牛乳房炎链球菌的分离鉴定及某些生物学特性的研究[D].内蒙古呼和浩特:内蒙古农业大学,2010:1-36.
[13]辛志浩,倪宏波.黑龙江省某奶牛场隐性乳房炎病原分离鉴定及药敏试验[J].现代畜牧兽医,2017(2):32-37.
[14]Khan I U,Hassan A A,Abdulmawjood A,et al.Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods[J].J Vet Sci,2003,4(3):213.
[15]Momtaz H,Safarpoor D F,Taktaz T,et al.Shiga toxin-producing Escherichia coli isolated from bovine mastitic milk:serogroups,virulence factors,and antibiotic resistance properties[J].Sci World J,2012(2):618709.
[16]黄现青,高晓平,赵改名.防腐剂复配优化抗藤黄微球菌研究[J].食品与机械,2010,26(6):57-59.
[17]倪珊珊,黄丽英.乳酸链球菌素和乳酸乳球菌在食品工业中的应用[J].食品工业,2015(11):244-247.
[2]车车.无乳链球菌、停乳链球菌、乳房链球菌GapC蛋白的表达与免疫原性研究[D].黑龙江大庆:黑龙江八一农业大学,2009:1-2.
[3]Preethirani P L,Isloor S,Sundareshan S.Isolation,biochemical and molecular identification,and in vitro antimicrobial resistance patterns of bacteria isolated from bubaline subclinical mastitis in South India[J].PLoS One,2015,10(11):e0142717.
[4]田会丽,袁筠,廖超.石河子地区乳源性金黄色葡萄球菌毒力及耐药性的研究[J].现代食品科技,2017(9):266.
[5]潘玉琨,谢珊珊,党斌,等.山羊临床型乳房炎主要病原菌分离鉴定及药敏试验[J].动物医学进展,2015,36(7):122-125.
[6]周义正,李向阳,邱小燕,等.PCR法快速检测阳性血培养瓶中葡萄球菌属[J].中华医院感染学杂志,2008,18(12):1798-1800.
[7]夏雪娟,郑炯,叶秀娟,等.实时荧光定量PCR技术监测腌制麻竹笋中乳酸乳球菌动态变化[J].食品科学,2016,37(4):88-92.
[8]胡翀,马薇,张可昕,等.多重PCR方法快速检测血流感染10种常见病原菌方法的建立与应用[J].中华医院感染学杂志,2018(7).
[9]胡勇.牦牛乳制品加工进展[J].青海畜牧兽医杂志,2009,39(1):44-45.
[10]刘诗吟.国际化发展的新机遇---2017中国牦牛乳产业发展论坛侧记[J].甘肃农业,2017(15):8-12.
[11]孙西峰,沈延银.牦牛隐性乳房炎发病情况的调查[J].青海畜牧兽医杂志,2009,39(3):10.
[12]白龙.呼市地区奶牛乳房炎链球菌的分离鉴定及某些生物学特性的研究[D].内蒙古呼和浩特:内蒙古农业大学,2010:1-36.
[13]辛志浩,倪宏波.黑龙江省某奶牛场隐性乳房炎病原分离鉴定及药敏试验[J].现代畜牧兽医,2017(2):32-37.
[14]Khan I U,Hassan A A,Abdulmawjood A,et al.Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods[J].J Vet Sci,2003,4(3):213.
[15]Momtaz H,Safarpoor D F,Taktaz T,et al.Shiga toxin-producing Escherichia coli isolated from bovine mastitic milk:serogroups,virulence factors,and antibiotic resistance properties[J].Sci World J,2012(2):618709.
[16]黄现青,高晓平,赵改名.防腐剂复配优化抗藤黄微球菌研究[J].食品与机械,2010,26(6):57-59.
[17]倪珊珊,黄丽英.乳酸链球菌素和乳酸乳球菌在食品工业中的应用[J].食品工业,2015(11):244-247.