两株生防细菌对苦瓜枯萎病的防治效果及其定殖密度的研究
|
摘要
以"如玉39"苦瓜为试材,利用皿内和盆栽试验,研究了生防细菌K18-5和KH48-5对苦瓜枯萎病菌的抑制活性和苦瓜枯萎病的防治效果,及其在苦瓜根系的定殖能力,以期为苦瓜枯萎病生物防治提供优质的生防资源。结果表明:K18-5和KH48-5均显著抑制枯萎病菌菌丝生长及其分生孢子的萌发;在盆栽试验中,K18-5和KH48-5对苦瓜枯萎病具有显著的防治效果,其防效分别达到75.27%和69.11%。K18-5和KH48-5均具有良好的根际定殖能力,在皿内采用双层滤纸法测定其定殖密度,分别为2.45×104 cfu·cm~(-1)和4.60×103 cfu·cm~(-1);在盆栽试验中,K18-5和KH48-5在苦瓜根部稳定保持1×103 cfu·g-1以上的定殖密度。
'Ruyu 39'bitter melon was used as material.In vitro and pot experiments were conducted to research on the inhibitory activities and control effect of two kinds of K18-5and KH48-5biocontrol agents against Fusarium oxysporumand their the root colonization abilities.In order to provide quality biological control resources for balsam pear wilt.The results showed that K18-5and KH48-5inhibited the mycelial growth and conidiospore germination of Fusarium oxysporum in vitro.In pot experiment,K18-5and KH48-5had significant control potentials against Fusarium wilt and the control efficiencies were 75.27% and 69.11% respectively.In addition,K18-5and KH48-5 had strong colonization abilities in rhizosphere of bitter melon,the colonization densities were 2.45×104 cfu·cm~(-1) and 4.60×103 cfu·cm~(-1) respectively with double layer filter paper method,in pot experiment,K18-5and KH48-5could sustain at high population densities in bitter melon root which were more than 1×103 cfu·g-1.
'Ruyu 39'bitter melon was used as material.In vitro and pot experiments were conducted to research on the inhibitory activities and control effect of two kinds of K18-5and KH48-5biocontrol agents against Fusarium oxysporumand their the root colonization abilities.In order to provide quality biological control resources for balsam pear wilt.The results showed that K18-5and KH48-5inhibited the mycelial growth and conidiospore germination of Fusarium oxysporum in vitro.In pot experiment,K18-5and KH48-5had significant control potentials against Fusarium wilt and the control efficiencies were 75.27% and 69.11% respectively.In addition,K18-5and KH48-5 had strong colonization abilities in rhizosphere of bitter melon,the colonization densities were 2.45×104 cfu·cm~(-1) and 4.60×103 cfu·cm~(-1) respectively with double layer filter paper method,in pot experiment,K18-5and KH48-5could sustain at high population densities in bitter melon root which were more than 1×103 cfu·g-1.
引文
[1]何美仙,罗军.苦瓜枯萎病非化学药剂防治技术研究进展[J].安徽农学通报,2013,19(11):49-51.
[2]殷晓敏,陈弟,郑服丛.尖镰孢枯萎病生物防治研究进展[J].广西农业科学,2008,39(2):172-178.
[3]纪明山,王英姿,程根武,等.西瓜枯萎病拮抗菌株筛选及田间防效试验[J].中国生物防治学报,2002,18(2):71-74.
[4]王刚,王俊芳,张颖,等.西瓜内生细菌C18的内生定殖及对西瓜枯萎病的生防作用[J].植物保护学报,2007,34(1):109-110.
[5]岳菊,刘邮洲,张荣胜,等.西瓜枯萎菌拮抗细菌的筛选、鉴定及防效测定[J].中国生物防治学报,2011,27(3):428-432.
[6]赵佳,黄静,陈哲,等.西瓜枯萎病拮抗菌Lh-1的鉴定及生物防治效果研究[J].生物技术通报,2017,33(4):130-136.
[7]张猛,王琼,冯发运,等.植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果[J].中国生物防治学报,2017,33(3):371-377.
[8]张璐,丁延芹,杜秉海,等.黄瓜枯萎病病原拮抗细菌DS-1菌株鉴定及其生防效果研究[J].园艺学报,2010,37(4):575-580.
[9]李晶,杨谦,张淑梅,等.枯草芽孢杆菌B29菌株防治黄瓜枯萎病的田间效果及安全性评价初报[J].中国蔬菜,2009(2):30-33.
[10]卜春亚,贾玉琦,王有年,等.黄瓜枯萎病生防芽孢杆菌的定向筛选及其抑菌活性物质特性分析[J].中国农学通报,2013,29(31):101-107.
[11]唐蕊,张雪辉,殷世群,等.黄瓜枯萎病拮抗菌HM-7的鉴定[J].中国瓜菜,2016,29(8):13-16.
[12]陈香,唐彤彤,孙星,等.对黄瓜枯萎病具防效的海洋源芽孢杆菌Y3F的鉴定[J].微生物学通报,2017,44(10):2370-2379.
[13]DU N S,SHI L,YUAN Y H,et al.Isolation of a potential biocontrol agent Paenibacillus polymyxa NSY50from vinegar waste compost and its induction of host defense responses against Fusarium wilt of cucumber[J].Microbiological Research,2017,202:1-10.
[14]高增贵,李天来,赵玥,等.甜瓜枯萎病拮抗内生细菌筛选[J].西北农林科技大学学报(自然科学版),2005,33(S1):85-87.
[15]庄敬华,杨长成,高增贵,等.枯草芽孢杆菌(Bacillus subtilis)B6对甜瓜枯萎病的生防作用[J].果树学报,2008,25(6):891-895.
[16]邹立飞,侯宪文,李光义,等.一株甜瓜枯萎病病原拮抗菌的筛选与防治效果初报[J].广东农业科学,2013,40(1):72-75,78.
[17]赵玉华,郝学政,文才艺.多黏类芽胞杆菌DP-11接种剂的制备及其对苦瓜枯萎病的防治效果[J].河南科技学院学报(自然科学版),2017,45(6):23-29.
[18]KLOEPPER J W,BEAUCHAMP C J.A review of issues related to measuring colonization of plant roots by bacteria[J].Canadian Journal of Microbiology,1992,38(12):1219-1232.
[19]安重莹,何晓明,谢大森,等.瓜类蔬菜枯萎病抗性分子标记研究进展[J].中国蔬菜,2009,1(8):7-10.
[20]佘小漫,何自福,罗方芳,等.瓜类枯萎病及其综合防治技术[J].广东农业科学,2011,38(2):84-87.
[21]杨威,刘苏闵,郭坚华.细菌定殖能力与其生物防治功能相关性研究进展[J].中国生物防治学报,2010,26(S1):90-94.
[22]徐刘平,尹燕妮,李师默,等.拮抗细菌对土传病原菌的作用机理[J].中国生物防治学报,2006,22(1):10-14.
[23]李长松.拮抗性细菌生物防治植物土传病害的研究进展[J].中国生物防治学报,1992,8(4):168-172.
[24]申顺善,赵玉华,张利敬,等.绿针假单胞菌HG28-5对辣椒疫病的抑制活性及其根际定殖特性的研究[J].园艺学报,2013,40(8):1574-1582.
[25]黎起秦,罗宽,林纬,等.内生菌B47的定殖能力及其对番茄青枯病的防治作用[J].植物保护学报,2006,33(4):363-368.
[2]殷晓敏,陈弟,郑服丛.尖镰孢枯萎病生物防治研究进展[J].广西农业科学,2008,39(2):172-178.
[3]纪明山,王英姿,程根武,等.西瓜枯萎病拮抗菌株筛选及田间防效试验[J].中国生物防治学报,2002,18(2):71-74.
[4]王刚,王俊芳,张颖,等.西瓜内生细菌C18的内生定殖及对西瓜枯萎病的生防作用[J].植物保护学报,2007,34(1):109-110.
[5]岳菊,刘邮洲,张荣胜,等.西瓜枯萎菌拮抗细菌的筛选、鉴定及防效测定[J].中国生物防治学报,2011,27(3):428-432.
[6]赵佳,黄静,陈哲,等.西瓜枯萎病拮抗菌Lh-1的鉴定及生物防治效果研究[J].生物技术通报,2017,33(4):130-136.
[7]张猛,王琼,冯发运,等.植物内生特基拉芽胞杆菌的分离、鉴定及防治西瓜枯萎病效果[J].中国生物防治学报,2017,33(3):371-377.
[8]张璐,丁延芹,杜秉海,等.黄瓜枯萎病病原拮抗细菌DS-1菌株鉴定及其生防效果研究[J].园艺学报,2010,37(4):575-580.
[9]李晶,杨谦,张淑梅,等.枯草芽孢杆菌B29菌株防治黄瓜枯萎病的田间效果及安全性评价初报[J].中国蔬菜,2009(2):30-33.
[10]卜春亚,贾玉琦,王有年,等.黄瓜枯萎病生防芽孢杆菌的定向筛选及其抑菌活性物质特性分析[J].中国农学通报,2013,29(31):101-107.
[11]唐蕊,张雪辉,殷世群,等.黄瓜枯萎病拮抗菌HM-7的鉴定[J].中国瓜菜,2016,29(8):13-16.
[12]陈香,唐彤彤,孙星,等.对黄瓜枯萎病具防效的海洋源芽孢杆菌Y3F的鉴定[J].微生物学通报,2017,44(10):2370-2379.
[13]DU N S,SHI L,YUAN Y H,et al.Isolation of a potential biocontrol agent Paenibacillus polymyxa NSY50from vinegar waste compost and its induction of host defense responses against Fusarium wilt of cucumber[J].Microbiological Research,2017,202:1-10.
[14]高增贵,李天来,赵玥,等.甜瓜枯萎病拮抗内生细菌筛选[J].西北农林科技大学学报(自然科学版),2005,33(S1):85-87.
[15]庄敬华,杨长成,高增贵,等.枯草芽孢杆菌(Bacillus subtilis)B6对甜瓜枯萎病的生防作用[J].果树学报,2008,25(6):891-895.
[16]邹立飞,侯宪文,李光义,等.一株甜瓜枯萎病病原拮抗菌的筛选与防治效果初报[J].广东农业科学,2013,40(1):72-75,78.
[17]赵玉华,郝学政,文才艺.多黏类芽胞杆菌DP-11接种剂的制备及其对苦瓜枯萎病的防治效果[J].河南科技学院学报(自然科学版),2017,45(6):23-29.
[18]KLOEPPER J W,BEAUCHAMP C J.A review of issues related to measuring colonization of plant roots by bacteria[J].Canadian Journal of Microbiology,1992,38(12):1219-1232.
[19]安重莹,何晓明,谢大森,等.瓜类蔬菜枯萎病抗性分子标记研究进展[J].中国蔬菜,2009,1(8):7-10.
[20]佘小漫,何自福,罗方芳,等.瓜类枯萎病及其综合防治技术[J].广东农业科学,2011,38(2):84-87.
[21]杨威,刘苏闵,郭坚华.细菌定殖能力与其生物防治功能相关性研究进展[J].中国生物防治学报,2010,26(S1):90-94.
[22]徐刘平,尹燕妮,李师默,等.拮抗细菌对土传病原菌的作用机理[J].中国生物防治学报,2006,22(1):10-14.
[23]李长松.拮抗性细菌生物防治植物土传病害的研究进展[J].中国生物防治学报,1992,8(4):168-172.
[24]申顺善,赵玉华,张利敬,等.绿针假单胞菌HG28-5对辣椒疫病的抑制活性及其根际定殖特性的研究[J].园艺学报,2013,40(8):1574-1582.
[25]黎起秦,罗宽,林纬,等.内生菌B47的定殖能力及其对番茄青枯病的防治作用[J].植物保护学报,2006,33(4):363-368.