转基因抗虫水稻秀水134-Bt毒蛋白的时空表达分析
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摘要
采用双抗夹心酶联免疫(ELISA)方法研究了转基因抗虫水稻秀水134-Bt不同生育期和不同组织中Bt毒蛋白的表达情况。结果表明:秀水134-Bt毒蛋白在各个组织中均有表达,但不同器官中表达量明显不同。Bt毒蛋白在叶片中表达量最高,叶鞘次之,根、茎、小穗中表达量相对较低,成熟种子中几乎检测不到。不同发育期叶片Bt毒蛋白的表达有显著差异,Bt毒蛋白表达量随着植株的生长而减少。研究结果可为转基因抗虫水稻适宜检测时间选择提供一定的参考。
The expression of Bt toxin protein in different growth stages and tissues of Xiushui 134-Bt wasstudied by double antibody sandwich ELISA. The results showed that Bt toxin protein of Xiushui 134-Bt wasexpressed in all tissues,but the expression level was different in different organs. The expression level of Btprotein in leaves was the highest,followed by leaf sheath. The expression level in roots,stems and spikelets wasrelatively low,and hardly detected in mature seeds. The expression of Bt toxin protein was significantly differentat different growth stages,and decreased with plant growth. The results could provide a reference for selectingsuitable detection time for transgenic insect-resistant rice.
The expression of Bt toxin protein in different growth stages and tissues of Xiushui 134-Bt wasstudied by double antibody sandwich ELISA. The results showed that Bt toxin protein of Xiushui 134-Bt wasexpressed in all tissues,but the expression level was different in different organs. The expression level of Btprotein in leaves was the highest,followed by leaf sheath. The expression level in roots,stems and spikelets wasrelatively low,and hardly detected in mature seeds. The expression of Bt toxin protein was significantly differentat different growth stages,and decreased with plant growth. The results could provide a reference for selectingsuitable detection time for transgenic insect-resistant rice.
引文
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[12]杨瑞芳,白建江,方军,等.转基因抗虫水稻秀水134-Bt和非转基因亲本的稻米主要品质性状与淀粉链长分布比较[J].上海农业学报,2017,33(1):31-34.
[13]YE R,HUANG H,YANG Z,et al.Development of insect-resistant transgenic rice with Cry1C*-free endosperm[J].Pest Manag Sci,2009,65(9):1015-1020.
[2]刘丽,于志晶,张文娟,等.转基因抗虫水稻研究进展[J].安徽农业科学,2011,3(9):5109.
[3]于志晶,刘丽,李淑芳,等.转Cry1C*基因抗虫水稻的培育[J].分子植物育种,2011,9(6):702-708.
[4]丁爽.转基因重大专项为我国农业可持续发展提供科技支撑[J].中国农村科技,2008(7):11.
[5]林冬枝,董彦君.Bt抗虫基因导入上海主栽粳稻品种‘秀水134’的研究[J].上海农业学报,2016,32(3):1-4.
[6]杨瑞芳,白建江,朴钟泽,等.转Cry1Ac1基因抗虫水稻的培育[J].分子植物育种,2014,12(6):1103-1111.
[7]刘金洋,江海洋,赵阳,等.转基因抗虫玉米转育株Bt蛋白的表达特征分析[J].安徽农业大学学报,2014,41(5):725-730.
[8]汪秀峰,叶芬,李莉,等.转基因水稻恢复系及其F1代Bt蛋白的时空表达分析[J].分子植物育种,2014,12(6):1077-1081.
[9]许文涛,白卫滨,罗云波,等.转基因产品检测技术研究进展[J].农业生物技术学报,2008,16(4):714-722.
[10]邓汉超,尹长城,刘国振,等.转基因植物核酸成分检测技术研究进展[J].中国生物工程杂志,2011,31(1):86-95.
[11]杨瑞芳,白建江,方军,等.转Cry1Ac1基因抗虫水稻和非转基因亲本的主要营养成分比较[J].江苏农业科学,2016,44(10):328-330.
[12]杨瑞芳,白建江,方军,等.转基因抗虫水稻秀水134-Bt和非转基因亲本的稻米主要品质性状与淀粉链长分布比较[J].上海农业学报,2017,33(1):31-34.
[13]YE R,HUANG H,YANG Z,et al.Development of insect-resistant transgenic rice with Cry1C*-free endosperm[J].Pest Manag Sci,2009,65(9):1015-1020.