丁香酚对兔痒螨的体外杀螨活性研究
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摘要
为了探讨丁香酚对兔痒螨的杀螨机制,试验用液体石蜡将丁香酚稀释成不同浓度,采用浸杀法测定丁香酚对兔痒螨成螨的半数致死浓度(median lethal concentration,LC_(50))和半数致死时间(median lethal time,LT_(50))。结果表明:4,2,1 mg/m L浓度的丁香酚均可在24 h内杀死全部供试螨;在2,4,8,12,18,24小时时,丁香酚杀螨的LC_(50)分别为3.219,2.289,0.872,0.695,0._(50)7,0.319 mg/m L;浓度为4,2,1,0.5,0.25,0.125 mg/m L时,丁香酚杀螨的LT_(50)分别为1.6,3.5,8.0,19.8,29.4,52.1 h。说明丁香酚具有显著的杀螨活性。
To investigate the acaricidal mechanism of eugenol on Psoroptes cuniculi( P. cuniculi),liquid paraffin was used to dilute eugenol at different concentrations. A dipping method was used to determine the median lethal concentration( LC_(50)) and median lethal time( LT_(50)) of eugenol to adult mites of P. cuniculi in vitro. The results showed that 4,2 and 1 mg / m L concentrations of eugenol could kill all tested mites within24 h. The values of LD_(50) of eugenol to P. cuniculi were 3. 219,2. 289,0. 872,0. 695,0. _(50)7 and 0. 319 mg / m L at 2,4,8,12,18 and24 h,respectively. While the values of LT_(50) at 4,2,1,0. 5,0. 25 and 0. 125 mg / m L concentrations of eugenol were 1. 6,3. 5,8. 0,19. 8,29. 4 and 52. 1 h,respectively. The results indicate that eugenol has a significantly acaricidal activity in vitro.
To investigate the acaricidal mechanism of eugenol on Psoroptes cuniculi( P. cuniculi),liquid paraffin was used to dilute eugenol at different concentrations. A dipping method was used to determine the median lethal concentration( LC_(50)) and median lethal time( LT_(50)) of eugenol to adult mites of P. cuniculi in vitro. The results showed that 4,2 and 1 mg / m L concentrations of eugenol could kill all tested mites within24 h. The values of LD_(50) of eugenol to P. cuniculi were 3. 219,2. 289,0. 872,0. 695,0. _(50)7 and 0. 319 mg / m L at 2,4,8,12,18 and24 h,respectively. While the values of LT_(50) at 4,2,1,0. 5,0. 25 and 0. 125 mg / m L concentrations of eugenol were 1. 6,3. 5,8. 0,19. 8,29. 4 and 52. 1 h,respectively. The results indicate that eugenol has a significantly acaricidal activity in vitro.
引文
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[12]贾春生.利用SPSS软件计算杀虫剂的LD50[J].昆虫知识,2006,43(3):414-417.
[13]PERRUCCI S,MACCHIONI G,CIONI P L,et al.Structure/activity relationship of some natural monoterpenes as acaricides against Psoroptes cuniculi[J].J Nat Prod,1995,58(8):1261-1264.
[14]熊凌锌,陈颖,钟留情,等.丁香酚对兔痒螨超微结构和酶活性的影响[J].动物医学进展,2013,34(10):72-75.
[2]柏克仁.民和县甘沟乡兔痒螨病调查[J].青海畜牧兽医杂志,2012,42(3):12.
[3]刘冬丽,刘冬辉.兔痒螨病的防治[J].中国畜禽种业,2010(4):100.
[4]周泐,尚丛珊,宋晓平.7-羟基-4-甲基香豆素衍生物杀螨活性研究[J].西北农业学报,2013,22(10):39-43.
[5]阮娜,宋晓平.丁香杀螨活性成分的追踪分离纯化与结构鉴定[J].中国农学通报,2005,21(9):24-27.
[6]GU X B,FANG C L,YANG G Y,et al.Acaricidal properties of an Ailanthus altissima bark extract against Psoroptes cuniculi and Sarcoptes scabiei var.cuniculi in vitro[J].Exp Appl Acarol,2014,62(2):225-232.
[7]贾小勇.水牛痒螨和兔痒螨形态学比较及ITS-2序列分析[D].雅安:四川农业大学,2007.
[8]邓云夏,王靖菲,殷中琼,等.4种中药单体物质的体外杀螨活性[J].中国兽医学报,2014,34(1):136-139,143.
[9]FICHI G,FLAMINI G,GIOVANELLI F,et al.Efficacy of an essentifal oil of Eugenia caryophyllata against Psoroptes cuniculi[J].Exp Parasitol,2007,115(2):168-172.
[10]SHANG X F,MIAO X L,WANG D S,et al.Acaricidal activity of extracts from Adonis coerulea Maxim.against Psoroptes cuniculi in vitro and in vivo[J].Vet Parasitol,2013,195(1/2):136-141.
[11]周永欣,章宗涉.水生生物毒性试验方法[M].北京:农业出版社,1989.
[12]贾春生.利用SPSS软件计算杀虫剂的LD50[J].昆虫知识,2006,43(3):414-417.
[13]PERRUCCI S,MACCHIONI G,CIONI P L,et al.Structure/activity relationship of some natural monoterpenes as acaricides against Psoroptes cuniculi[J].J Nat Prod,1995,58(8):1261-1264.
[14]熊凌锌,陈颖,钟留情,等.丁香酚对兔痒螨超微结构和酶活性的影响[J].动物医学进展,2013,34(10):72-75.