条浒苔破壁方法研究
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摘要
为了建立一种有效的条浒苔细胞壁破壁方法,以显微观察和蛋白质提取率为评价指标,考察干法超微粉碎法、湿法超微粉碎法、超声波粉碎法、湿法超微粉碎+酶解法和超声波粉碎+酶解法5种方法对条浒苔细胞壁的破坏效果.结果表明,5种方法中湿法超微粉碎+酶解法处理后,细胞组织形态被破坏,细胞之间排布零散无规律,细胞内组织液流出,此方法蛋白质提取率最高,可达到30.86%.
In order to establish an effective cell wall breaking method for Enteromorpha clathrata,five methods which include dry superfine pulverization,wet ultrafine pulverization,ultrasonic pulverization,ultrasonic pulverization with enzymatic hydrolysis and wet ultrafine pulverization with enzymatic hydrolysis are investigated to evaluate the effect of destruction on the cell wall of Enteromorpha clathrata by using microscopic observation and the protein extraction rate as index.The results demonstrate that the method of wet ultrafine pulverization with enzymatic hydrolysis treatment can breakdown the cell morphous,make the cells scatter irregularly and the enchylema is flowed out.The extraction rate of protein treated by this method can reach 30.86%,which is the highest among the tested methods.
In order to establish an effective cell wall breaking method for Enteromorpha clathrata,five methods which include dry superfine pulverization,wet ultrafine pulverization,ultrasonic pulverization,ultrasonic pulverization with enzymatic hydrolysis and wet ultrafine pulverization with enzymatic hydrolysis are investigated to evaluate the effect of destruction on the cell wall of Enteromorpha clathrata by using microscopic observation and the protein extraction rate as index.The results demonstrate that the method of wet ultrafine pulverization with enzymatic hydrolysis treatment can breakdown the cell morphous,make the cells scatter irregularly and the enchylema is flowed out.The extraction rate of protein treated by this method can reach 30.86%,which is the highest among the tested methods.
引文
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[2]于洋,刘迎迎,王建勇,等.日照近岸海域浒苔暴发区浮游植物调查研究[J].海洋开发与管理,2017(11):74-78.
[3]何清,胡晓波,周峙苗,等.东海绿藻缘管浒苔营养成分分析及评价[J].海洋科学,2006,30(1):34-38.
[4]徐大伦,黄晓春,杨文鸽,等.浒苔营养成分分析[J].浙江海洋学院学报(自然科学版),2003,22(4):318-320.
[5]廖梅杰,郝志凯,尚德荣,等.浒苔营养成分分析与投喂刺参试验[J].渔业现代化,2011,38(4):32-36.
[6]KIM D Y,VIJAYAN D,PRAVEENKUMAR R,et al.Cell-wall disruption and lipid/astaxanthin extraction from microalgae:Chlorella and Haematococcus[J].Bioresource Technology,2016,199(1):300-310.
[7]朱江,赖达雄,欧阳邦进.破壁绿藻粉的加工及细胞生长因子的提取研究[J].江西食品工业,2006(2):26-28.
[8]HAGEN C,SIEGMUND S,BRAUNE W.Ultrastructural and chemical changes in the cell wall of Haematococcus pluvialis(Volvocales,Chlorophyta)during aplanospore formation[J].European Journal of Phycology,2002,37(2):217-226.
[9]肖海芳,马海乐,孙进良,等.不同破壁方法对条斑紫菜藻红蛋白提取效果的影响[J].食品研究与开发,2006,27(10):54-56.
[10]任晓丽,陈林,刘天中,等.破壁方法对雨生红球藻湿藻生物质中虾青素提取的影响[J].中国食品学报,2018,18(12):110-117.
[11]姜剑锋,赵丽芹,陈涛,等.寇氏隐甲藻不同破壁方法的研究[J].中国粮油学报,2011,26(8):92-95.
[12]盘赛昆,姚东瑞,朱强,等.条浒苔蛋白质的纤维素酶辅助提取及其性质[J].渔业科学进展,2013,34(4):109-117.
[13]中华人民共和国卫生部.食品中蛋白质的测定:GB5009.5-2010[S].北京:中国标准出版社,2010.