基于线粒体COI和16S rRNA基因的中国沿海相手蟹系统发育研究
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摘要
相手蟹科的诸多种类因其形态极其相似成为方蟹总科分类中疑问较多的一个类群。通过对中国沿海相手蟹线粒体COI和16S rRNA基因序列进行分子系统发育分析,结果表明14种相手蟹COI和16S rRNA基因序列之间差异分别为5.7%～14.5%和1.5%～12.1%,均达到了种间差异水平。构建的系统发育树显示,14种相手蟹分别为独立有效物种,但分属于拟相手蟹属和近相手蟹属的4种拟相手蟹和3种近相手蟹,没有分别形成2个独立的支系,而是混合聚成一大支系。而属于螳臂相手蟹属的无齿螳臂相手蟹则首先与属于中相手蟹属的中华中相手蟹聚成一支,再与红螯螳臂相手蟹聚为一大支,表现出与形态分类的不一致。错综复杂的分子系统关系预示着相手蟹类为多系起源,也表明它们之间的种间关系乃至于属间关系尚有诸多问题有待进一步厘定。
The species in the family of Sesarmidae are among the species mostly difficult to be differentiated taxonomically in the Superfamily Grapsoidea because of their morphological similarity. The analysis of partial sequences of mitochondrial COI gene and 16 S rRNA gene of 14 species in 8 genera of Sesarmidae for their molecular phylogenetics reveals that the percentages of sequence divergences of 14 species of Sesarmidae are from 5.7% to14.5% and from 1.5% to 12.1%, both indicating that the species difference are significant. The phylogenetic trees show that the 14 species are separate and valid species and 4 species of Parasesarma and 3 species of Perisesarma are not 2 independent clades but clustered into 1 clade. Chiromantes dehaani and Sesarmops sinensis are clustered into one clade and then clustered with C. haematocheir into another clade, inconsistent with their morphological classification. The complicated molecular phylogenetic relationship among the species of Sesarmidae suggests that the species in the family of Sesarmidae might have polyphyletic origin, and their interspecific relationship or intergenus relationship need to be clarified.
The species in the family of Sesarmidae are among the species mostly difficult to be differentiated taxonomically in the Superfamily Grapsoidea because of their morphological similarity. The analysis of partial sequences of mitochondrial COI gene and 16 S rRNA gene of 14 species in 8 genera of Sesarmidae for their molecular phylogenetics reveals that the percentages of sequence divergences of 14 species of Sesarmidae are from 5.7% to14.5% and from 1.5% to 12.1%, both indicating that the species difference are significant. The phylogenetic trees show that the 14 species are separate and valid species and 4 species of Parasesarma and 3 species of Perisesarma are not 2 independent clades but clustered into 1 clade. Chiromantes dehaani and Sesarmops sinensis are clustered into one clade and then clustered with C. haematocheir into another clade, inconsistent with their morphological classification. The complicated molecular phylogenetic relationship among the species of Sesarmidae suggests that the species in the family of Sesarmidae might have polyphyletic origin, and their interspecific relationship or intergenus relationship need to be clarified.
引文
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[18]Rahayu D L,Ng P K L.Revision of the Parasesarma plicatum(Latreille,1803)species-group(Crustacea:Decapoda:Brachyura:Sesarmidae)[J].Zootaxa,2010,2327(1):1-22.
[19]Folmer O,Black M,Hoeh W,et al.DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J].Molecular Marine Biology and Biotechnology,1994,3(5):294-299.
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[24]徐敬明.厚蟹遗传多样性与分类地位的研究[D].青岛:中国海洋大学,2007.Xu Jingming.Studies on genetic diversity and taxonomic status of the crab genus Helice[D].Qingdao:Ocean University of China,2007.
[25]Xing Yuhui,Ma Xiaoping,Wei Yuqing,et al.The complete mitochondrial genome of the semiterrestrial crab,Chiromantes neglectum(Eubrachyura:Grapsoidea:Sesarmidae)[J].Mitochondrial DNA Part B,2016,1(1):461-463.
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[27]Liu Xue,Yuan Yiming,He Yanlong,et al.Characterization of the complete mitochondrial genome of Sesarma dehaani with phylogenetic analysis[J].Mitochondrial DNA Part B,2019,4(1):350-351.
[28]Kawane M,Wada K,Watanabe K.Comparisons of genetic population structures in four intertidal brachyuran species of contrasting habitat characteristics[J].Marine Biology,2008,156(2):193-203.
[29]Komai T,Ng P K L.A new species of sesarmid crab of the genus Chiromantes(Crustacea:Decapoda:Brachyura)from the Ogasawara Islands,Japan[J].Zootaxa,2013,3681(5):539-551.
[2]戴爱云,杨思谅,宋玉枝,等.中国海洋蟹类[M].北京:海洋出版社,1986:482-512.Dai Aiyun,Yang Siliang,Song Yuzhi,et al.Crabs of the China Seas[M].Beijing:China Ocean Press,1986:482-512.
[3]刘瑞玉.中国海洋生物名录[M].北京:科学出版社,2008:801-802.Liu Ruiyu.Checklist of Marine Biota of China Seas[M].Beijing:Science Press,2008:801-802.
[4]杨明柳,徐敬明,吴斌,等.北部湾红树林蟹类多样性初步研究[J].四川动物,2014,33(3):347-352,357.Yang Mingliu,Xu Jingming,Wu Bin,et al.A preliminary study on crabs diversity for Beibu Gulf mangrove[J].Sichuan Journal of Zoology,2014,33(3):347-352,357.
[5]杨明柳,徐敬明,吴斌,等.基于形态和16S rRNA基因的拟相手蟹分类研究[J].海洋学报,2016,38(8):62-71.Yang Mingliu,Xu Jingming,Wu Bin,et al.Taxonomic study on the genus Parasesarma based on the morphology characteristics and mtDNA 16S rRNA gene[J].Haiyang Xuebao,2016,38(8):62-71.
[6]梁晓莉.长江河口大型底栖动物疑难种修订及河口种形成机理初探[D].上海:华东师范大学,2017.Liang Xiaoli.Revision of doubtful marcrobenthic species and the preliminary study of formation mechanism of estuarine species in the Yangtze Estuary[D].Shanghai:East China Normal University,2017.
[7]Moritz C,Dowling T E,Brown W M.Evolution of animal mitochondrial DNA:relevance for population biology and systematics[J].Annual Review of Ecology and Systematics,1987,18:269-292.
[8]徐敬明.蟹类线粒体DNA的研究与应用[J].中国海洋大学学报,2006,36(6):879-884.Xu Jingming.Study and application of mitochondrial DNA in crabs[J].Periodical of Ocean University of China,2006,36(6):879-884.
[9]Schubart C D,Diesel R,Hedges S B.Rapid evolution to terrestrial life in Jamaican crabs[J].Nature,1998,393(6683):363-365.
[10]Harrison J S.Evolution,biogeography,and the utility of mitochondrial 16S and COI genes in phylogenetic analysis of the crab genus Austinixa(Decapoda:Pinnotheridae)[J].Molecular Phylogenetics and Evolution,2004,30(3):743-754.
[11]徐敬明,方华华,高天翔.两种相手蟹线粒体COI基因序列的比较研究[J].海洋通报,2007,26(6):26-31.Xu Jingming,Fang Huahua,Gao Tianxiang.Sequence comparison of the mitochondrial COI gene between two sesarma species[J].Marine Science Bulletin,2007,26(6):26-31.
[12]Xin Zhaozhe,Liu Yu,Tang Boping,et al.A comprehensive phylogenetic analysis of Grapsoidea crabs(Decapoda:Brachyura)based on mitochondrial cytochrome oxidase subunit 1(CO1)genes[J].Turkish Journal of Zoology,2018,42(1):46-52.
[13]Shahdadi A,Schubart C D.Taxonomic review of Perisesarma(Decapoda:Brachyura:Sesarmidae)and closely related genera based on morphology and molecular phylogenetics:new classification,two new genera and the questionable phylogenetic value of the epibranchial tooth[J].Zoological Journal of the Linnean Society,2018,182(3):517-548.
[14]Davie P J F,Pabriks L.A new species of Parasesarma(Crustacea:Brachyura:Sesarmidae)from the mangroves of Western Australia[J].Zootaxa,2010,2564(1):62-68.
[15]Koller P,Liu H,Schubart C D.A new semiterrestrial species of Parasesarma De Man,1895,from Taiwan(Decapoda,Brachyura,Sesarmidae)[M]//Fransen C H J M,De Grave S,Ng P K L.Studies on Malacostraca:Lipke Bijdeley Holthuis Memorial Volume.Crustaceana Monographs.Boston:Brill,2010:357-368.
[16]Rahayu D L,Ng P K L.Two new species of Parasesarma De Man,1895,from Southeast Asia(Crustacea:Decapoda:Brachyura:Sesarmidae)[J].Zootaxa,2009,1980:29-40.
[17]Naderloo R,Schubart C D.Description of a new species of Parasesarma(Crustacea;Decapoda;Brachyura;Sesarmidae)from the Persian Gulf,based on morphological and genetic characteristics[J].Zoologischer Anzeiger:A Journal of Comparative Zoology,2010,249(1):33-43.
[18]Rahayu D L,Ng P K L.Revision of the Parasesarma plicatum(Latreille,1803)species-group(Crustacea:Decapoda:Brachyura:Sesarmidae)[J].Zootaxa,2010,2327(1):1-22.
[19]Folmer O,Black M,Hoeh W,et al.DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J].Molecular Marine Biology and Biotechnology,1994,3(5):294-299.
[20]Palumbi S R.Genetic divergence,reproductive isolation,and marine speciation[J].Annual Review of Ecology and Systematics,1994,25:547-572.
[21]Miller M A,Pfeiffer W,Schwartz T.Creating the CIPRES Science Gateway for inference of large phylogenetic trees[C]//Proceedings of2010 Gateway Computing Environments Workshop(GCE).New Orleans,LA:IEEE,2010:1-8.
[22]Ronquist F,Huelsenbeck J P.MrBayes 3:Bayesian phylogenetic inference under mixed models[J].Bioinformatics,2003,19(12):1572-1574.
[23]Shih H T,Suzuki H.Taxonomy,phylogeny,and biogeography of the endemic mudflat crab Helice/Chasmagnathus complex(Crustacea:Brachyura:Varunidae)from East Asia[J].Zoological Studies,2008,47(1):114-125.
[24]徐敬明.厚蟹遗传多样性与分类地位的研究[D].青岛:中国海洋大学,2007.Xu Jingming.Studies on genetic diversity and taxonomic status of the crab genus Helice[D].Qingdao:Ocean University of China,2007.
[25]Xing Yuhui,Ma Xiaoping,Wei Yuqing,et al.The complete mitochondrial genome of the semiterrestrial crab,Chiromantes neglectum(Eubrachyura:Grapsoidea:Sesarmidae)[J].Mitochondrial DNA Part B,2016,1(1):461-463.
[26]Schubart C D,Koller P.Genetic diversity of freshwater crabs(Brachyura:Sesarmidae)from central Jamaica with description of a new species[J].Journal of Natural History,2005,39(6):469-481.
[27]Liu Xue,Yuan Yiming,He Yanlong,et al.Characterization of the complete mitochondrial genome of Sesarma dehaani with phylogenetic analysis[J].Mitochondrial DNA Part B,2019,4(1):350-351.
[28]Kawane M,Wada K,Watanabe K.Comparisons of genetic population structures in four intertidal brachyuran species of contrasting habitat characteristics[J].Marine Biology,2008,156(2):193-203.
[29]Komai T,Ng P K L.A new species of sesarmid crab of the genus Chiromantes(Crustacea:Decapoda:Brachyura)from the Ogasawara Islands,Japan[J].Zootaxa,2013,3681(5):539-551.