摘要
本试验旨在研究沙葱、沙葱水溶性提取物和脂溶性提取物对肉羊瘤胃发酵及微生物区系的影响。试验选用24只体况良好、体重为(37.1±0.5) kg的杜寒F1杂交羊,随机分为4组,每组6个重复,每个重复1只羊。对照组饲喂基础饲粮,沙葱组在基础饲粮中添加沙葱粉[10.0 g/(d·只)],脂溶组在基础饲粮中添加沙葱脂溶性提取物[2.8 g/(d·只)],水溶组在基础饲粮中添加沙葱水溶性提取物[3.4 g/(d·只)]。饲养试验共持续75 d,其中预试期15 d,正试期60 d;在正试期的第0、30和60天晨饲后2 h通过口腔采集瘤胃液测定瘤胃发酵参数和瘤胃微生物的含量。结果表明:1)与对照组相比,在60 d时,饲粮中添加沙葱及其提取物显著提高了瘤胃液pH(P<0.05),降低了瘤胃液氨态氮的浓度(P>0.05),但均未超过正常范围;饲粮中添加沙葱显著提高了瘤胃液菌体蛋白浓度(P<0.05)。2)与对照组相比,在60 d时,饲粮中添加沙葱和其脂溶性提取物显著提高了瘤胃液丙酸浓度(P<0.05),添加沙葱显著提高了瘤胃液丁酸浓度(P<0.05)并显著降低了异丁酸的浓度(P<0.05),乙酸、戊酸和异戊酸浓度各组间差异不显著(P>0.05)。3)与对照组相比,在30、60 d时,饲粮中添加沙葱及其提取物显著提高了产琥珀酸丝状杆菌和黄色瘤胃球菌含量(P<0.05),对溶纤维丁酸弧菌、甲烷菌、白色瘤胃球菌含量没有显著影响(P>0.05);在60 d时,饲粮中添加沙葱水溶性提取物显著提高瘤胃液真菌含量(P<0.05),饲粮中添加沙葱显著降低瘤胃液原虫含量(P<0.05)。综上,沙葱及其提取物改善了瘤胃发酵模式,显著影响了肉羊瘤胃微生物区系。
The purpose of this experiment was to study the effects of the Allium monogolium Regel( AMR),water-soluble extracts( AWE) and fat-soluble extracts( AFE) of AMR on rumen fermentation and rumen microflora of sheep. Twelve Dorper × thin-tailed Han crossbred with body weight of( 37. 1 ± 0. 5) kg were randomly divided into 4 groups with 1 repetition per group. The control( CON) group was fed a basal diet,the AMR group was fed diets supplemented with AMR( 10 g/d per sheep),the AFE group was fed diets supplemented with AFE( 2.8 g/d per sheep),the AWE group was fed diets supplemented with AWE( 3.4 g/d per sheep). The feeding test lasted 75 d including a 15-day pre-test period and a 60-day formal period. Rumen fermentation parameters and rumen microbial content were measured by oral rumen fluid collection after feeding2 h later on the days 0,30 and 60 of the feeding period. The results showed as follows: 1) compared with the CON group,on day 60,dietary AMR and its extracts significantly increased the pH of the rumen fluid( P<0.05),and decreased the concentration of ammonia nitrogen in the rumen fluid( P> 0. 05); the addition of AMR in the diet significantly increased the concentration of mycoprotein in the rumen fluid( P < 0. 05). 2)Compared with the CON group,on day 60,the addition of AMR and AFE in the diet significantly increased the concentration of propionic acid in the rumen fluid( P<0.05),the addition of AMR in the diet significantly increased the concentration of butyric acid in the rumen fluid( P<0.05) and significantly decreased the concentration of isobutyric acid( P<0.05),the differences in the concentrations of acetic acid,valeric acid and isovaleric acid were not significant among groups( P>0.05). 3) Compared with the CON group,on days 30 and60,dietary AMR and its extracts significantly increased the contents of Ruminococcus succinogenes and Ruminococcus flavefaciens in the rumen fluid( P<0.05) and had no significant effect on the contents of Butyrivibrio fibrisolvens,methanogen and Ruminococcus albus( P>0.05); on day 60,dietary AWE significantly increased the fungi content in the rumen fluid( P<0.05); dietary AWE significantly decreased the content of protozoa in the rumen fluid( P<0.05). In summary,dietary AMR and its extract improve the rumen fermentation mode and the rumen microflora of sheep.[Chinese Journal of Animal Nutrition,2019,31( 5) : 2313-2322]
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