红三叶新品系生理生化指标对低温的响应机理
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摘要
为研究红三叶(Trifolium pratense)新品系(R)的耐低温性,本试验以岷山红三叶(CK1)和甘红1号红三叶(CK2)品种为对照,采用人工模拟低温环境的方法对其进行4℃低温胁迫,测定了各参试材料的叶片含水量(Content of leaf water,LWC),叶绿素含量(Chlorophyll,CHL),可溶性糖含量(Soluble Sugar,SS),丙二醛含量(Malondialdehyde,MDA),以及叶片超氧化物歧化酶(Superoxide Dismutase,SOD),过氧化物酶(Peroxidase,POD)和过氧化氢酶(Catalase,CAT)活性。结果表明,随着低温胁迫时间延长,3个红三叶材料叶片的LWC和CHL含量逐渐降低,MDA含量和SS含量则逐渐升高,POD,SOD和CAT活性均呈先升高后下降的变化趋势;3个参试材料中,R的LWC和SS含量最低,CHL,MDA含量及叶片SOD,POD和CAT活性最高;从红三叶材料×低温胁迫天数交互作用而言,R的LWC下降幅度较大,CHL含量显著高于CK2(P<0.05),叶片SOD,POD和CAT活性显著高于2对照材料(P<0.05)。采用隶属函数法对3个红三叶材料的耐低温性综合评价表明,3个红三叶材料耐低温性强弱依次为R>CK1>CK2。
In order to study the low temperature resistance of red clover line(R),Trifolium pretense cv.Minshan and Ganhong No.1 were used as controls,the leaf water content(LWC),chlorophyll content(CHL),soluble sugar content(SS),malondialdehyde content(MDA),and activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)in red clover were measured under 4 ℃ artificial simulation.The results showed that contents of LWC and CHL of 3 red clover leaves were decreased gradually with the prolong of low temperature stress.Contents of MDA and SS increased gradually,and activities of POD,SOD and CAT increased first and then decreased.Among 3 materials,R had the lowest contents of LWC and SS,contents of CHL and MDA,and activities of SOD,POD and CAT in leaf were the highest.From the interaction of red clover materials and low temperature stress days,the LWC of R decreased greatly,CHL content was significantly higher than that of CK2(P<0.05),activity of SOD,POD and CAT was significantly higher than that of the controls(P<0.05).Comprehensive evaluation based on the membership function method showed that R had the strongest resistance to low temperature,followed by R > CK1 and CK2.
In order to study the low temperature resistance of red clover line(R),Trifolium pretense cv.Minshan and Ganhong No.1 were used as controls,the leaf water content(LWC),chlorophyll content(CHL),soluble sugar content(SS),malondialdehyde content(MDA),and activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)in red clover were measured under 4 ℃ artificial simulation.The results showed that contents of LWC and CHL of 3 red clover leaves were decreased gradually with the prolong of low temperature stress.Contents of MDA and SS increased gradually,and activities of POD,SOD and CAT increased first and then decreased.Among 3 materials,R had the lowest contents of LWC and SS,contents of CHL and MDA,and activities of SOD,POD and CAT in leaf were the highest.From the interaction of red clover materials and low temperature stress days,the LWC of R decreased greatly,CHL content was significantly higher than that of CK2(P<0.05),activity of SOD,POD and CAT was significantly higher than that of the controls(P<0.05).Comprehensive evaluation based on the membership function method showed that R had the strongest resistance to low temperature,followed by R > CK1 and CK2.
引文
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[2]刘艳芝,夏彤,王玉民,等.8个苜蓿品种抗寒性的初步评价[J].吉林农业科学,2007,32(1):17-18
[3]金雅琴.乌桕实生苗培育及耐盐抗旱生理研究[D].南京:南京林业大学,2012:23-27
[4]金波.五种常绿攀缘藤本植物抗寒性研究[D].南京:南京林业大学,2007:9-19
[5]和红云,田丽萍,薛琳.植物抗寒性生理生化研究进展[J].天津农业科学,2007,13(2):10-13
[6]韩涛,赵宏伟,贾琰,等.孕穗期低温胁迫对寒地粳稻功能叶片SOD、POD和CAT活性的影响[J].东北农业科学,2015,40(6):21-25
[7]孔令慧.红三叶株型结构与生产性能相关性分析及其抗逆性研究[D].兰州:甘肃农业大学,2013:4-36
[8]崔英.三种三叶草的耐盐性和抗寒性研究[D].内蒙古:内蒙古农业大学,2006:10-13
[9]王晓军,于凤芝,高洪生,等.四种三叶草肥饲价值及耐寒性的比较研究[J].黑龙江农业科学,2015(10):137-140
[10]张元科.蒙农1号红三叶特征特性研究[D].内蒙古:内蒙古农业大学,2014:19-25
[11]邹琦主编.植物生理学实验指导[M].北京:中国农业出版社,2003:11-166
[12]乔富廉主编.植物生理学实验分析测定技术[M].北京:中国农业科技出版社,2002:52-101
[13]罗尧幸,郭荣荣,李雪雪,等.基于隶属函数法评价7个鲜食葡萄品种的抗寒性[J].贵州农业科学,2018,46(6):38-44
[14]柯碧英.南洋楹不同种源抗寒性的初步研究[D].广州:华南农业大学,2002:1-5
[15]孔令慧,赵桂琴.不同品种红三叶苗期对4℃低温胁迫的生理响应[J].中国草地学报,2013,35(3):31-37
[16]Gerloff E D,Stahmann M A,Smith D.Soluble proteins in alfalfa roots as related to cold hardiness[J].Plant Physiology,1967,42(7):895-899
[17]刘瑞媛,董喜存,梁开平,等.不同品种甜高粱苗期耐寒性分析研究[J].中国农学通报,2017,34(31):19-26
[18]王苗.接种根瘤菌对紫花苜蓿抗寒性的影响[D].西北农林科技大学,2015:15-21
[19]王宁,徐杰,吴军,等.喷施不同外源物质对低温胁迫下的光蜡树苗部分抗寒生理指标的影响[J].西部林业科学,2010,39(1):31-35
[20]申晓慧,姜成,冯鹏,等.六种紫花苜蓿在高寒地区抗寒生理特性差异比较研究[J].草地学报,2016,24(5):1131-1133
[21]赵一鹤,李建宾,杨时宇,等.低温胁迫下3个甜角品种幼苗的抗寒性研究[J].林业科学研究,2012,25(2):150-156
[22]德英,赵来喜,穆怀彬.30份老芒麦种质材料抗寒性研究[J].草业科学,2011,28(1):90-93
[23]吉春容,邹陈,陈丛敏,等.低温胁迫下巴旦杏的形态生理变化及抗寒性研究[J].中国沙漠,2012,32(4):955-962
[24]陈代慧,刘宇新,王艳梅,等.低温胁迫对不同抗寒品种山茶花生理生化指标的影响[J].河南科学,2013,(12):2154-2159
[25]Bowley S R,Mckersie B D.Relationships among freezing,low temperature flooding,and ice encasement tolerance in alfalfa.[J].Canadian Journal of Plant Science,1990,70(1):227-235
[26]刘雨诗,耿金才,宋江湖,等.根瘤共生对紫花苜蓿抗寒性的影响[J].草地学报,2018,26(3):631-638