大头典竹扦插过程中营养物质和氧化酶活性变化研究
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摘要
以2年生大头典竹(Dendrocalamopsis beecheyana)的粗壮主侧枝为实验材料,采用600 mg·L~(-1)的ABT1溶液预先处理5 min,以m(黄泥土)∶m(泥炭土)=1∶1混合为基质,进行扦插,研究不同扦插时期大头典竹生根特性和扦插生根后可溶性糖、可溶性蛋白和相关抗氧化酶活性变化。结果表明:600 mg·L~(-1)的ABT1处理对扦插生根具有重要影响,处理后的插条生根率最高可达69.84%。大头典竹侧枝扦插生根属于潜伏不定根原基生根型,具体过程分为根的诱导时期(0~14 d)、表达时期(14~21 d)和伸长时期(21~35 d)3个阶段。扦插后,可溶性糖质量分数呈现先降低后上升再下降的变化趋势,可溶性蛋白质量分数则呈现相反变化趋势。在扦插生根过程中氧化酶活性呈规律性变化,过氧化物酶(POD)、吲哚乙酸氧化酶(IAAO)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)活性在不定根诱导时期呈上升趋势;在不定根表达形成期,POD、PPO活性下降,IAAO活性急剧上升,SOD活性保持稳定;在不定根的伸长时期,IAAO和PPO活性下降,POD活性先上升后下降,SOD活性仍维持较高水平。
To establish a commercial feasible cutting propagation system,the branches of Dendrocalamopsis beecheyana were used as experimental materials,the cuttings were pretreated with 600 mg·L~(-1) ABT1 for 5 min,then planted in the mixed substrate of yellow soil and peat(1∶ 1). The rooting characteristics were observed and the contents of soluble sugar,soluble protein and oxidase activities in the cuttings were measured after rooting. The results showed that ABT1 with 600 mg·L~(-1) had significant effects on rooting of cuttings,and the rooting rate of cuttings reached 69. 84%. They rooted from the latent adventitious root primordium,and the rooting process can be divided into induction period(0-14 d),expression period(14-21 d)and elongation period(21-35 d). After plantation of the cuttings,the content of soluble sugar decreased first,then increased,and decreased at last. The content of soluble protein showedthe opposite trend. The activity of peroxidase( POD), indoleacetic oxidase( IAAO),Polyphenol oxidase( PPO) and superoxide dismutase( SOD) increased at the root induction stage. During the root formation stage,the activity of POD and PPO decreased,the activity of IAAO increased sharply,and the activities of SOD remained stable. In the root elongation phase,the activities of IAAO and PPO decreased,the activity of POD increased first and then decreased,and the activity of SOD remained high.
To establish a commercial feasible cutting propagation system,the branches of Dendrocalamopsis beecheyana were used as experimental materials,the cuttings were pretreated with 600 mg·L~(-1) ABT1 for 5 min,then planted in the mixed substrate of yellow soil and peat(1∶ 1). The rooting characteristics were observed and the contents of soluble sugar,soluble protein and oxidase activities in the cuttings were measured after rooting. The results showed that ABT1 with 600 mg·L~(-1) had significant effects on rooting of cuttings,and the rooting rate of cuttings reached 69. 84%. They rooted from the latent adventitious root primordium,and the rooting process can be divided into induction period(0-14 d),expression period(14-21 d)and elongation period(21-35 d). After plantation of the cuttings,the content of soluble sugar decreased first,then increased,and decreased at last. The content of soluble protein showedthe opposite trend. The activity of peroxidase( POD), indoleacetic oxidase( IAAO),Polyphenol oxidase( PPO) and superoxide dismutase( SOD) increased at the root induction stage. During the root formation stage,the activity of POD and PPO decreased,the activity of IAAO increased sharply,and the activities of SOD remained stable. In the root elongation phase,the activities of IAAO and PPO decreased,the activity of POD increased first and then decreased,and the activity of SOD remained high.
引文
[1]孙中元,马艳军,高健.慈竹竹枝扦插及埋节育苗技术[J].东北林业大学学报,2013,(9):14-18.
[2]金爱武,方伟,余学军,等.ABT生根粉处理绿竹、吊丝单竹主枝扦插试验[J].林业科技开发,2001,(4):31-33.
[3]王青天.雷竹侧枝育苗及造林技术试验[J].林业科技开发,2003,17(z1):23-24.
[4]唐廷贵.麻竹扦插繁育技术研究[J].四川林勘设计,2003(3):24-28.
[5]刘延青.仿栗扦插繁殖技术及其生根机理的研究[D].长沙:中南林业科技大学学报,2010.
[6]黄卓烈,李明,詹福建,等.不同生长素处理对桉树无性系插条氧化酶活性影响的比较研究[J],林业科学,2002,38(4):46-52.
[7]华宏,杜旭华,王顺财,等.楸树扦插生根过程中插条保护酶与丙二醛动态变化[J].南京林业大学学报(自然科学版),2008,32(6):128-132.
[8]Hausman J F,Evers D,Kevers C,et al.Internal controls of root induction in poplar shoots raised in vitro[J].Angewandte Botanik,1997.
[9]扈红军,曹帮华,尹伟伦.榛子嫩枝扦插生根相关氧化酶活性变化及繁殖技术[J].林业科学,2008,44(6):60-65.
[10]高俊凤.植物生理学[M].北京:高等教育出版社,2006:142,144.
[11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,1999:195.
[12]张志良,瞿伟菁,李小芳.植物生理学实验指导[M].北京:高等教育出版社.2009:155.
[13]扈红军.榛子扦插生根机理与繁殖技术的研究[D].泰安:山东农业大学,2011.
[14]王新建,何威,张秋娟,等.豫楸1号扦插生根过程中营养物质含量及氧化酶类活性的变化[J].林业科学,2009,45(4):156-161.
[15]MONCOUSIN C H,GASPAR T.Peroxidase as a marker for rooting improvement in Cynara scolymus L.cultivated in vitro[J].Biochemie Und Physiologie Der Pflanzen,1983,178:263-271.
[16]HAISSIG B E.Influences of auxins and suxin synergists on adventitious root primordium initiation and development[J].New Zealand Journal of Forestry Science,1974,4:311-323.
[17]GYANA G R.Effects of auxins on adventitious root development from single node cuttings of Camellia sinensis(L.)Kuntze and associated biochemical changes[J].Plant Growth Regul,2006,48:111-117.
[18]宋丽红,曹帮华.光叶楮扦插生根的吲哚乙酸氧化酶、多酚氧化酶、过氧化物酶活性变化研究[J].武汉植物学研究,2005,23(4):347-350.
[19]王小玲,赵忠,权金娥.外源激素对四倍体刺槐硬枝扦插生根及其关联酶活性的影响[J].西北植物学报,2011,31(1):116-122.
[20]马振华,赵忠,张晓鹏,等.四倍体刺槐扦插生根过程中氧化酶活性的变化[J].西北农林科技大学学报(自然科学版),2007,35(7):85-89.
[21]李明,黄卓烈,谭绍满.难易生根按树多酚氧化酶,吲哚乙酸氧化酶活性及其同工酶的比较研究[J].林业科学研究,2000,13(5):493-500.
[22]刘明鲁,孙磊,张晓峰,等.桑树硬枝扦插生根过程中相关生理生化指标的动态分析[J].2011,32(4):9-14
[23]田敏,饶龙兵,李纪元.植物细胞中的活性氧及其生理作用[J].植物生理学通讯,2005,41(2):235-238.
[24]尹万元.光叶榉扦插繁殖技术及生根过程中生理变化[D].泰安:山东农业大学,2016.
[25]姜志强.榉树扦插繁殖技术与生根机理的研究[D].南京:南京林业大学,2008.
[26]张忠微,石素霞,张彦广,等.金露梅嫩枝插穗生根过程中营养物质含量变化研究[J].河北农业大学学报.2008,31(4):56-59.
[27]李志辉,刘延青,张斌.K-IBA处理对仿栗秋季扦插生根的影响[J].中国农学通报,2010,26(16):109-112.
[28]GEBHARDT K.Activation of indole-3-actic acid oxidase from horseradish and prunus by phenols and hydrogen peroxide[J].Plant Growth Regulation,1982,1(2):73-84.
[29]HAISSIF B E.Metabolic processes in adventitious rooting of cuttings[G]//JACKSON M B.New Root Formation in Plant and Cuttings.Lancaster:Martinus Nijhoff,1986:141-189.
[30]宋金耀,何文林,李松波,等.毛白杨嵌合体扦插生根相关理化特性分析[J].林业科学,2001,37(5):64-67.
[31]王瑞,陈永忠,王湘南,等.油茶组培苗生根过程中生理生化指标的变化[J].经济林研究,2015,33(2):68-72.
[32]郑科,郎南军,曹福亮,等.麻风树枝条扦插生长中多酚氧化酶、过氧化物酶活性变化[J].贵州农业科学,2010,38(4):177-180.
[33]赵云龙,陈训,李朝婵.糙叶杜鹃扦插生根过程中生理生化分析[J].林业科学,2013,49(6):45-51.
[2]金爱武,方伟,余学军,等.ABT生根粉处理绿竹、吊丝单竹主枝扦插试验[J].林业科技开发,2001,(4):31-33.
[3]王青天.雷竹侧枝育苗及造林技术试验[J].林业科技开发,2003,17(z1):23-24.
[4]唐廷贵.麻竹扦插繁育技术研究[J].四川林勘设计,2003(3):24-28.
[5]刘延青.仿栗扦插繁殖技术及其生根机理的研究[D].长沙:中南林业科技大学学报,2010.
[6]黄卓烈,李明,詹福建,等.不同生长素处理对桉树无性系插条氧化酶活性影响的比较研究[J],林业科学,2002,38(4):46-52.
[7]华宏,杜旭华,王顺财,等.楸树扦插生根过程中插条保护酶与丙二醛动态变化[J].南京林业大学学报(自然科学版),2008,32(6):128-132.
[8]Hausman J F,Evers D,Kevers C,et al.Internal controls of root induction in poplar shoots raised in vitro[J].Angewandte Botanik,1997.
[9]扈红军,曹帮华,尹伟伦.榛子嫩枝扦插生根相关氧化酶活性变化及繁殖技术[J].林业科学,2008,44(6):60-65.
[10]高俊凤.植物生理学[M].北京:高等教育出版社,2006:142,144.
[11]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,1999:195.
[12]张志良,瞿伟菁,李小芳.植物生理学实验指导[M].北京:高等教育出版社.2009:155.
[13]扈红军.榛子扦插生根机理与繁殖技术的研究[D].泰安:山东农业大学,2011.
[14]王新建,何威,张秋娟,等.豫楸1号扦插生根过程中营养物质含量及氧化酶类活性的变化[J].林业科学,2009,45(4):156-161.
[15]MONCOUSIN C H,GASPAR T.Peroxidase as a marker for rooting improvement in Cynara scolymus L.cultivated in vitro[J].Biochemie Und Physiologie Der Pflanzen,1983,178:263-271.
[16]HAISSIG B E.Influences of auxins and suxin synergists on adventitious root primordium initiation and development[J].New Zealand Journal of Forestry Science,1974,4:311-323.
[17]GYANA G R.Effects of auxins on adventitious root development from single node cuttings of Camellia sinensis(L.)Kuntze and associated biochemical changes[J].Plant Growth Regul,2006,48:111-117.
[18]宋丽红,曹帮华.光叶楮扦插生根的吲哚乙酸氧化酶、多酚氧化酶、过氧化物酶活性变化研究[J].武汉植物学研究,2005,23(4):347-350.
[19]王小玲,赵忠,权金娥.外源激素对四倍体刺槐硬枝扦插生根及其关联酶活性的影响[J].西北植物学报,2011,31(1):116-122.
[20]马振华,赵忠,张晓鹏,等.四倍体刺槐扦插生根过程中氧化酶活性的变化[J].西北农林科技大学学报(自然科学版),2007,35(7):85-89.
[21]李明,黄卓烈,谭绍满.难易生根按树多酚氧化酶,吲哚乙酸氧化酶活性及其同工酶的比较研究[J].林业科学研究,2000,13(5):493-500.
[22]刘明鲁,孙磊,张晓峰,等.桑树硬枝扦插生根过程中相关生理生化指标的动态分析[J].2011,32(4):9-14
[23]田敏,饶龙兵,李纪元.植物细胞中的活性氧及其生理作用[J].植物生理学通讯,2005,41(2):235-238.
[24]尹万元.光叶榉扦插繁殖技术及生根过程中生理变化[D].泰安:山东农业大学,2016.
[25]姜志强.榉树扦插繁殖技术与生根机理的研究[D].南京:南京林业大学,2008.
[26]张忠微,石素霞,张彦广,等.金露梅嫩枝插穗生根过程中营养物质含量变化研究[J].河北农业大学学报.2008,31(4):56-59.
[27]李志辉,刘延青,张斌.K-IBA处理对仿栗秋季扦插生根的影响[J].中国农学通报,2010,26(16):109-112.
[28]GEBHARDT K.Activation of indole-3-actic acid oxidase from horseradish and prunus by phenols and hydrogen peroxide[J].Plant Growth Regulation,1982,1(2):73-84.
[29]HAISSIF B E.Metabolic processes in adventitious rooting of cuttings[G]//JACKSON M B.New Root Formation in Plant and Cuttings.Lancaster:Martinus Nijhoff,1986:141-189.
[30]宋金耀,何文林,李松波,等.毛白杨嵌合体扦插生根相关理化特性分析[J].林业科学,2001,37(5):64-67.
[31]王瑞,陈永忠,王湘南,等.油茶组培苗生根过程中生理生化指标的变化[J].经济林研究,2015,33(2):68-72.
[32]郑科,郎南军,曹福亮,等.麻风树枝条扦插生长中多酚氧化酶、过氧化物酶活性变化[J].贵州农业科学,2010,38(4):177-180.
[33]赵云龙,陈训,李朝婵.糙叶杜鹃扦插生根过程中生理生化分析[J].林业科学,2013,49(6):45-51.