去势对成年雄性斑胸草雀HVC神经元电生理特性的影响
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摘要
运用全细胞膜片钳记录法,在离体细胞水平研究了去势引起的雄激素水平降低对HVC不同神经元电生理特性的影响.研究结果显示:去势组与对照组相比,投射神经元HVCRA膜时间常数明显下降(df=38,P<0.01),输入电阻降低(df=35,P<0.01),动作电位后超极化幅值(df=43,P<0.05)及达到峰值时间(df=43,P<0.01)延长.投射神经元HVCx的相关指标变化情况与HVCRA神经元大致类似,差异表现在去势后投射神经元HVCX动作电位延时显著增加(df=55,P<0.01),显示雄激素可以提高2类投射神经元的兴奋性.研究结果表明:雄激素可以在一定程度上提高HVC神经元的兴奋性,雄激素可通过增强HVC对发声运动通路(Vocal Motor Pathway,VMP)的控制,抑制前端脑通路(Anterior Forebrain Pathway,AFP)来实现维持鸣曲的稳定.
This study investigates the effect of castration on electrophysiological properties of neurons in the HVC of adult male zebra finches. The electrophysiological properties of HVC neurons of the castrated bird were recorded with patch clamp. It was found that membrane time constants( df = 38,P<0.01) and input resistance( df = 35,P<0.01) of HVC projection neurons—HVCRA—in the castration group were lower than those in the control group. Afterhyperpolarization( AHP) time to peak( df = 43,P<0.05) and amplitude of action potential( df = 43,P <0.01)was prolonged after castration. As for HVCX,the changes in the related indexes were similar to those of the HVCRA neurons. The difference lies in that the action potential delay of the HVCXneurons after the castration was significantly increased( df = 55,P<0.01),suggesting that the androgens could improve the excitability of the two types of projective neurons. In conclusion,androgens can enhance the excitability of HVC projection neurons. They can activate the VMP pathway and inhibit the AFP pathway to maintain the stability of the song.
This study investigates the effect of castration on electrophysiological properties of neurons in the HVC of adult male zebra finches. The electrophysiological properties of HVC neurons of the castrated bird were recorded with patch clamp. It was found that membrane time constants( df = 38,P<0.01) and input resistance( df = 35,P<0.01) of HVC projection neurons—HVCRA—in the castration group were lower than those in the control group. Afterhyperpolarization( AHP) time to peak( df = 43,P<0.05) and amplitude of action potential( df = 43,P <0.01)was prolonged after castration. As for HVCX,the changes in the related indexes were similar to those of the HVCRA neurons. The difference lies in that the action potential delay of the HVCXneurons after the castration was significantly increased( df = 55,P<0.01),suggesting that the androgens could improve the excitability of the two types of projective neurons. In conclusion,androgens can enhance the excitability of HVC projection neurons. They can activate the VMP pathway and inhibit the AFP pathway to maintain the stability of the song.
引文
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[8] FUJIMOTO H,HASEGAWA T,WATANABE D. Neural coding of syntactic structure in learned vocalizations in the songbird[J]. Journal of Neuroscience,2011,31(27):10023-10033.
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[10]李东风,冯理,刘少艺,等.界面核和高级发声中枢与鸣禽鸣曲的学习和维持[J].华南师范大学学报(自然科学版),2013,45(6):140-147.LI D F,FENG L,LIU S Y,et al. Connections of NIf-HVC and vocal learning and maintenance in Songbirds[J].Journal of South China Normal University(Natural Science Edition),2013,45(6):140-147.
[11] THOMPSON C K. Cell death and the song control system:a model for how sex steroid hormones regulate naturally-occurring neurodegeneration[J]. Development Growth&Differentiation,2011,53(2):213-224.
[12]张近春,王松华,李东风.损毁LMAN逆转去势引起的成年雄鸟鸣曲变化[J].华南师范大学学报(自然科学版),2016,48(5):58-62.ZHANG J C,WANG S H,LI D F. Castrating-induced song change in adult male zebra finch(Taeniogygia guttata)is reversed by LMAN lesion[J]. Journal of South China Normal University(Natural Science Edition),2016,48(5):58-62.
[13] AMADOR A,PERL Y S,MINDLIN G B,et al. Elemental gesture dynamics are encoded by song premotor cortical neurons[J]. Nature,2013,495(7439):59-64.
[14] AMADOR A,PERL Y S,MINDLIN G B,et al. Elemental gesture dynamics are encoded by song premotor cortical neurons[J]. Nature,2013,495(7439):59-64.
[15] NOTTEBOHM F. The neural basis of birdsong[J]. PLoS Biology,2005,3(5):e164.
[16] SOBER S J,WOHLGEMUTH M J,BRAINARD M S.Central contributions to acoustic variation in birdsong[J].Journal of Neuroscience,2008,28(41):10370-10379.
[17] THOMPSON C K,BRENOWITZ E A. Seasonal change in neuron size and spacing but not neuronal recruitment in a basal ganglia nucleus in the avian song control system[J]. Journal of Comparative Neurology,2005,481(3):276-283.
[2] MEITZEN J,WEAVER A L,BRENOWITZ E A,et al.Plastic and stable electrophysiological properties of adult avian forebrain song-control neurons across changing breeding conditions[J]. Journal of Neuroscience,2009,29(20):6558-6567.
[3] MOONEY R. Neurobiology of song learning[J]. Current Opinion in Neurobiology,2009,19(6):654-660.
[4] DAOU A,ROSS M T,JOHNSON F,et al. Electrophysiological characterization and computational models of HVC neurons in the zebra finch[J]. Journal of Neurophysiology,2013,110(5):1227-1245.
[5] DUTAR P,VU H M,PERKEL D J. Multiple cell types distinguished by physiological,pharmacological,and anatomic properties in nucleus HVc of the adult zebra finch[J]. Journal of Neurophysiology,1998,80(4):1828-1838.
[6] MOONEY R,PRATHER J F. The HVC microcircuit:the synaptic basis for interactions between song motor and vocal plasticity pathways[J]. Journal of Neuroscience,2005,25(8):1952-1964.
[7] HERZ A V,GOLLISCH T,MACHENS C K,et al. Modeling single-neuron dynamics and computations:a balance of detail and abstraction[J]. Science,2006,314(5796):80-85.
[8] FUJIMOTO H,HASEGAWA T,WATANABE D. Neural coding of syntactic structure in learned vocalizations in the songbird[J]. Journal of Neuroscience,2011,31(27):10023-10033.
[9] HAMAGUCHI K,TSCHIDA K A,YOON I,et al. Auditory synapses to song premotor neurons are gated off during vocalization in zebra finches[J]. Elife,2014,3:e1833.
[10]李东风,冯理,刘少艺,等.界面核和高级发声中枢与鸣禽鸣曲的学习和维持[J].华南师范大学学报(自然科学版),2013,45(6):140-147.LI D F,FENG L,LIU S Y,et al. Connections of NIf-HVC and vocal learning and maintenance in Songbirds[J].Journal of South China Normal University(Natural Science Edition),2013,45(6):140-147.
[11] THOMPSON C K. Cell death and the song control system:a model for how sex steroid hormones regulate naturally-occurring neurodegeneration[J]. Development Growth&Differentiation,2011,53(2):213-224.
[12]张近春,王松华,李东风.损毁LMAN逆转去势引起的成年雄鸟鸣曲变化[J].华南师范大学学报(自然科学版),2016,48(5):58-62.ZHANG J C,WANG S H,LI D F. Castrating-induced song change in adult male zebra finch(Taeniogygia guttata)is reversed by LMAN lesion[J]. Journal of South China Normal University(Natural Science Edition),2016,48(5):58-62.
[13] AMADOR A,PERL Y S,MINDLIN G B,et al. Elemental gesture dynamics are encoded by song premotor cortical neurons[J]. Nature,2013,495(7439):59-64.
[14] AMADOR A,PERL Y S,MINDLIN G B,et al. Elemental gesture dynamics are encoded by song premotor cortical neurons[J]. Nature,2013,495(7439):59-64.
[15] NOTTEBOHM F. The neural basis of birdsong[J]. PLoS Biology,2005,3(5):e164.
[16] SOBER S J,WOHLGEMUTH M J,BRAINARD M S.Central contributions to acoustic variation in birdsong[J].Journal of Neuroscience,2008,28(41):10370-10379.
[17] THOMPSON C K,BRENOWITZ E A. Seasonal change in neuron size and spacing but not neuronal recruitment in a basal ganglia nucleus in the avian song control system[J]. Journal of Comparative Neurology,2005,481(3):276-283.