RAFT聚合法合成蛋白质分子印迹聚合物研究进展
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摘要
制备具有高选择性和亲和性的分子印迹聚合物(MIPs)是分子印迹技术发展的主要目标。以蛋白质等生物大分子为模板的分子印迹技术,面临蛋白质传质阻力大和蛋白质分子印迹聚合物形成的印迹位点不精确的问题。可逆加成-断裂链转移(RAFT)聚合法作为活性可控自由基聚合的一种方法,单体选择范围广且反应条件温和。通过RAFT法制备得到的MIPs具有结构可控,形成位点均一等优点,有利于形成高质量的精确位点,增加选择性和亲和性。
The preparation of molecularly imprinted polymers with high selectivity and affinity is the main objective in developing molecular imprinting technique. Molecular imprinting technique of proteins and other biomacromolecules is challenged by the difficulties in molecular diffusion and formation of high-quality imprints. As a kind of controlled radical polymerization, reversible-addition fragmentation chain transfer(RAFT) polymerization possesses properties of wide range of suitable monomers and mild reaction condition. The MIPs prepared by RAFT polymerization with controlled structures and homogeneity is beneficial to formation of high-quality accurate imprinting sites, which has advantages of improving selectivity and affinity.
The preparation of molecularly imprinted polymers with high selectivity and affinity is the main objective in developing molecular imprinting technique. Molecular imprinting technique of proteins and other biomacromolecules is challenged by the difficulties in molecular diffusion and formation of high-quality imprints. As a kind of controlled radical polymerization, reversible-addition fragmentation chain transfer(RAFT) polymerization possesses properties of wide range of suitable monomers and mild reaction condition. The MIPs prepared by RAFT polymerization with controlled structures and homogeneity is beneficial to formation of high-quality accurate imprinting sites, which has advantages of improving selectivity and affinity.
引文
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[24]Liu Y B,Fang S M,Zhai J Q,et al.Construction of antibody-like nanoparticles for selective protein sequestration in living cells[J].NANOSCALE,2015,7(16):7162-7167.
[25]Moraes J,Ohno K,Maschmeyer T,et al.Synthesis of silica-polymer core-shell nanoparticles by reversible addition-fragmentation chain transfer polymerization[J].Chemical Communications,2013,49(80):9077-9088.
[26]Qian L W,Hu X L,Guan P,et al.Thermal preparation of lysozyme-imprinted microspheres by using ionic liquid as a stabilizer[J].Analytical and Bioanalytical Chemistry,2014,406(28):7221-7231.
[27]Gong X,Tang B,Liu J J,et al.Synthesis of adenosine-imprinted microspheres for the recognition of ADP-ribosylated proteins[J].2017,84:858-864.
[28]Li Q R,Yang K G,Liang Y,et al.Surface Protein Imprinted Core-Shell Particles for High Selective Lysozyme Recognition Prepared by Reversible Addition-Fragmentation Chain Transfer Strategy[J].ACSAppl Mater Interfaces,2014,6(24):21954-21960.
[29]Boitard C,Rollet A L,Menager C,et al.Surface-initiated synthesis of bulk-imprinted magnetic polymers for protein recognition[J].Chem Commun(Camb),2017,53(63):8846-8849.
[2]韩霜.一种新兴的印迹聚合物制备方法─表面印迹法[J].当代化工,2015,44(10):2360-2362.
[3]Kubo T,Otsuka,K.Recent progress in molecularly imprinted media by new preparation concepts and methodological approaches for selective separation of targeting compounds[J].Trends in Analytical Chemistry,2016,81:102-109.
[4]Liu D,Zhao K Y,Qi M,et al.Preparation of protein molecular-imprinted polysiloxane membrane using calcium alginate film as matrix and its application for cell culture[J].Polymers,2018,10(2):170.
[5]宋欢语,赵孔银,李思迪,等.厚度可控蛋白质印迹海藻酸钙水凝胶膜的制备和表征[J].中国科学:技术科学,2016,46(09):931-939.
[6]董明杰,李义雅,柴志华,等.纳米结构蛋白质分子印迹聚合物的研究进展[J].高分子通报,2013(01):127-136.
[7]Boysen R I,Schwarz L J,Nicolau D V,et al.Molecularly imprinted polymer membranes and thin films for the separation and sensing of biomacromolecules[J].J Sep Sci,2017,40(1):314-335.
[8]Li D Y,Zhang X M,Yan Y J,et al.Thermo-sensitive imprinted polymer embedded carbon dots using epitope approach[J].Biosensors and Bioelectronics,2016,79,187-192.
[9]Du C B,Hu X L,Guan P,et al.Preparation of surface-imprinted microspheres effectively controlled by orientated template immobilization using highly cross-linked raspberry-like microspheres for the selective recognition of an immunostimulating peptide[J].Journal of Materials Chemistry B,2016,4(8):1510-1519.
[10]翟晓瑞,邓启良,袁士芳,等.表面印迹技术用于蛋白质的识别研究[J].食品研究与开发,2013,34(24):272-275.
[11]Takeuchi T,Kitayama Y,Sasao R,et al.Molecularly imprinted nanogels acquire stealth in situ by cloaking themselves with native dysopsonic proteins[J].Angewandte Chemie-International Edition,2017,56(25):7088-7092.
[12]Zuo P L,Gao J F,Peng J,et al.A sol-gel based molecular imprint incorporating carbon dots for fluorometric determination of nicotinic acid[J].Microchim Acta,2016,183(1):329-336.
[13]Hu R,Luan J Y,Kharasch E D,et al.Aromatic Functionality of Target Proteins Influences Monomer Selection for Creating Artificial Antibodies on Plasmonic Biosensors[J].ACS Appl Mater Interfaces,2017,9(1):145-151.
[14]Bouri M,Lerma-Garcia M J,Salghi R,et al.Selective extraction and determination of catecholamines in urine samples by using a dopamine magnetic molecularly imprinted polymer and capillary electrophoresis[J].Talanta,2012,99:897-903.
[15]Li Y X,Chen Y T,Huang L,et al.Creating BHb-imprinted magnetic nanoparticles with multiple binding sites[J].Analyst,2017,142(2):302-309.
[16]He H,Fu G,Wang Y,et al.Imprinting of protein over silica nanoparticles via surface graft copolymerization using low monomer concentration[J].Biosens Bioelectron,2010,26(2):760-765.
[17]Beyazit S,Bui B T S,Haupt K,et al.Molecularly imprinted polymer nanomaterials and nanocomposites by controlled/living radical polymerization[J].Progress In Polymer Science,2016,62:1-21.
[18]Zhang H Q.Recent Advances in Macromolecularly Imprinted Polymers by Controlled Radical Polymerization Techniques[J].Molecular Imprinting,2015,3(1).
[19]吴云霞,黄静,尹争志,等.悬浮聚合法制备罗丹明B磁性分子印迹微球及其性能[J].应用化学,2013,30(12):1481-1488.
[20]Yang,H,Guo,T Y,Zhou D Z.Surface hydrophilic modification with well-defined glycopolymer for protein imprinting matrix[J].International Journal of Biological Macromolecules,2011,48(3):432-438.
[21]Pardeshi S,Singh S K.Precipitation polymerization:a versatile tool for preparing molecularly imprinted polymer beads for chromatography applications[J].2016,6(28):23525-23536.
[22]Liu J X,Yang K G,Qu Y Y,et al.An efficient approach to prepare boronate core-shell polymer nanoparticles for glycoprotein recognition via combined distillation precipitation polymerization and RAFT media precipitation polymerization[J].Chem Commun(Camb),2015,51(18):3896-3898.
[23]李沁然,杨开广,李森武,等.可逆加成-断裂链转移策略制备可识别转铁蛋白的抗原决定基印迹微球[J].色谱,2014,32(10):1029-1033.
[24]Liu Y B,Fang S M,Zhai J Q,et al.Construction of antibody-like nanoparticles for selective protein sequestration in living cells[J].NANOSCALE,2015,7(16):7162-7167.
[25]Moraes J,Ohno K,Maschmeyer T,et al.Synthesis of silica-polymer core-shell nanoparticles by reversible addition-fragmentation chain transfer polymerization[J].Chemical Communications,2013,49(80):9077-9088.
[26]Qian L W,Hu X L,Guan P,et al.Thermal preparation of lysozyme-imprinted microspheres by using ionic liquid as a stabilizer[J].Analytical and Bioanalytical Chemistry,2014,406(28):7221-7231.
[27]Gong X,Tang B,Liu J J,et al.Synthesis of adenosine-imprinted microspheres for the recognition of ADP-ribosylated proteins[J].2017,84:858-864.
[28]Li Q R,Yang K G,Liang Y,et al.Surface Protein Imprinted Core-Shell Particles for High Selective Lysozyme Recognition Prepared by Reversible Addition-Fragmentation Chain Transfer Strategy[J].ACSAppl Mater Interfaces,2014,6(24):21954-21960.
[29]Boitard C,Rollet A L,Menager C,et al.Surface-initiated synthesis of bulk-imprinted magnetic polymers for protein recognition[J].Chem Commun(Camb),2017,53(63):8846-8849.