碲化镉量子点对A549细胞的毒性研究
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摘要
目的探讨碲化镉(CdTe)量子点的生物安全性。方法以水热法制备的、巯基丁二酸(MSA)包被的CdTe量子点为研究对象,作用于体外培养的A549细胞。实验设立对照组和不同浓度CdTe量子点作用组。CdTe量子点作用于细胞后,采用倒置荧光显微镜观察量子点作用下的细胞形态;使用alamar Blue检测CdTe量子点对细胞存活率的影响;使用Hoechst 33342单染法观察CdTe量子点对A549细胞核形态的影响;使用Annexin V-FITC单染法检测CdTe量子点对A549细胞凋亡的影响。结果 CdTe量子点作用后,低浓度(2.5,5,10 mg/L)实验组细胞变化不明显,高浓度实验组(25,50 mg/L)中的细胞肿胀变圆,细胞数量减少;存活率显著下降;在染色下可见明显的细胞凋亡。结论 MSA包被的CdTe量子点在达到一定浓度后对A549细胞有较强的毒性。这可能与量子点的纳米特性、CdTe的金属化合物毒性有关。
Objective It is to approach the biological security of CdTe QDs.Methods CdTe QDs which was made by hydrothermal method and covered by Mercapto succinic acid( MSA) was chosen as the object.The cells were divided into control group and CdTe QDs-treated groups with different concentrations of CdTe QDs.After affected by CdTe QDs,the morphology of A549 cells was observed by inverted fluorescence microscope.The cell survival rate was examined by alamarBlue,and nuclear morphology in staining was detected by Hoechst 33342.The influence of CdTe QDs on the apoptosis of A549 was examined by Annexin V-FITC single-stain procedure.Results Quantum dots can inhibit the proliferation of A549 cell in a dose-dependent manner.QDs of different concentrations will induce apoptosis in different degree.Conclusion CdTe QDs coated with MSA has strong toxicity to A549 cells in certain concentration.This may be related to nano characteristics of quantum dots and the metal toxicity of CdTe.
Objective It is to approach the biological security of CdTe QDs.Methods CdTe QDs which was made by hydrothermal method and covered by Mercapto succinic acid( MSA) was chosen as the object.The cells were divided into control group and CdTe QDs-treated groups with different concentrations of CdTe QDs.After affected by CdTe QDs,the morphology of A549 cells was observed by inverted fluorescence microscope.The cell survival rate was examined by alamarBlue,and nuclear morphology in staining was detected by Hoechst 33342.The influence of CdTe QDs on the apoptosis of A549 was examined by Annexin V-FITC single-stain procedure.Results Quantum dots can inhibit the proliferation of A549 cell in a dose-dependent manner.QDs of different concentrations will induce apoptosis in different degree.Conclusion CdTe QDs coated with MSA has strong toxicity to A549 cells in certain concentration.This may be related to nano characteristics of quantum dots and the metal toxicity of CdTe.
引文
[1]Nguyen KC,Seligy VL,Tayabali AF.Cadmium telluride quantum dot nanoparticle cytotoxicity and effects on model immune responses to Pseudomonas aeruginosa[J].Nanotoxicology,2013,7(2):202-211
[2]Song YC,Duan F,Wen S,et al.Parallel comparative studies on the toxic effects of unmodified CdTe quantum dots,gold nanoparticles,and carbon nanodots on live cells as well as green gram sprouts[J].Talanta,2013,116(14):237-244
[3]Nguyen KC,Willmore WG,Tayabali AF.Cadmium telluride quantum dots cause oxidative stress leading to extrinsic and intrinsic apoptosis in hepatocellular carcinoma HepG2 cells[J].Toxicology,2013,306(6):114-123
[4]王翔,闫蕾,贾光等.纳米材料潜在健康影响的研究进展[J].毒理学杂志,2005,19(1):15-17
[5]王琳,王莉娟,张芳,等.纳米氧化锌对人肺腺癌细胞A549的毒性[J].中国科学院研究生院学报,2009,26(1):83-90
[6]许臻,李冬梅,姚亚玲,等.硫脲修饰法制备高发光性能CdTe量子点[J].物理化学学报,2009,25(6):1201-1206
[7]严明.碲化镉量子点对血管内皮细胞的毒性及相关机制研究[D].浙江大学,2011
[8]杨阳,刘宝瑞,钱晓萍,等.Alamar Blue法用于体外培养细胞活性检测的方法研究[J].现代肿瘤医学,2006,14(1):6-8
[9]刘锡娟,丁慧荣,张宏,等.用DAPI和Hoechst33342染色法检测DNA的流式细胞方法探讨[J].北京大学学报:医学版,2010,42(4):480-484
[10]陈大明,金小海,罗志福,等.放射性Annexin V活体检测细胞凋亡的研究进展[J].原子能科学技术,2004,38(1):35-42
[11]朱霖,刘锰,沈锐,等.Annexin V在核医学细胞凋亡显像中的应用[J].中华核医学杂志,2004,24(6):379-381
[12]祁本忠,陈大明,杨红伟,等.细胞凋亡显像剂99Tcm-HYNICAnnexinⅤ的制备及其生物评价[J].同位素,2006,19(3):140-145
[13]刘闽碧.用Excel做t检验与F检验[J].海峡预防医学杂志,2002,8(5):67-68
[14]张云娇.特异性表面结合肽调控稀土纳米材料自噬和毒性的研究[D].中国科学技术大学,2012
[15]张勤丽,牛侨.细胞凋亡机制概述[J].环境与职业医学,2007,24(1):102-107
[16]汤睿,朱正纲.凋亡途径与肿瘤治疗[J].世界华人消化杂志,2005,13(20):2469-2472
[17]李超,伏圣博,刘华玲,等.细胞凋亡研究进展[J].世界科技研究与发展,2007,29(3):45-53
[2]Song YC,Duan F,Wen S,et al.Parallel comparative studies on the toxic effects of unmodified CdTe quantum dots,gold nanoparticles,and carbon nanodots on live cells as well as green gram sprouts[J].Talanta,2013,116(14):237-244
[3]Nguyen KC,Willmore WG,Tayabali AF.Cadmium telluride quantum dots cause oxidative stress leading to extrinsic and intrinsic apoptosis in hepatocellular carcinoma HepG2 cells[J].Toxicology,2013,306(6):114-123
[4]王翔,闫蕾,贾光等.纳米材料潜在健康影响的研究进展[J].毒理学杂志,2005,19(1):15-17
[5]王琳,王莉娟,张芳,等.纳米氧化锌对人肺腺癌细胞A549的毒性[J].中国科学院研究生院学报,2009,26(1):83-90
[6]许臻,李冬梅,姚亚玲,等.硫脲修饰法制备高发光性能CdTe量子点[J].物理化学学报,2009,25(6):1201-1206
[7]严明.碲化镉量子点对血管内皮细胞的毒性及相关机制研究[D].浙江大学,2011
[8]杨阳,刘宝瑞,钱晓萍,等.Alamar Blue法用于体外培养细胞活性检测的方法研究[J].现代肿瘤医学,2006,14(1):6-8
[9]刘锡娟,丁慧荣,张宏,等.用DAPI和Hoechst33342染色法检测DNA的流式细胞方法探讨[J].北京大学学报:医学版,2010,42(4):480-484
[10]陈大明,金小海,罗志福,等.放射性Annexin V活体检测细胞凋亡的研究进展[J].原子能科学技术,2004,38(1):35-42
[11]朱霖,刘锰,沈锐,等.Annexin V在核医学细胞凋亡显像中的应用[J].中华核医学杂志,2004,24(6):379-381
[12]祁本忠,陈大明,杨红伟,等.细胞凋亡显像剂99Tcm-HYNICAnnexinⅤ的制备及其生物评价[J].同位素,2006,19(3):140-145
[13]刘闽碧.用Excel做t检验与F检验[J].海峡预防医学杂志,2002,8(5):67-68
[14]张云娇.特异性表面结合肽调控稀土纳米材料自噬和毒性的研究[D].中国科学技术大学,2012
[15]张勤丽,牛侨.细胞凋亡机制概述[J].环境与职业医学,2007,24(1):102-107
[16]汤睿,朱正纲.凋亡途径与肿瘤治疗[J].世界华人消化杂志,2005,13(20):2469-2472
[17]李超,伏圣博,刘华玲,等.细胞凋亡研究进展[J].世界科技研究与发展,2007,29(3):45-53