碲化镉量子点对PC-12细胞的细胞毒性研究
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摘要
目的:探讨碲化镉(CdTe)量子点的生物安全性。方法:以水热法制备的、巯基丁二酸(MSA)包被的碲化镉(CdTe)量子点为研究对象,作用于体外培养的鼠神经元细胞(PC-12)。实验设立对照组和不同浓度CdTe QDs作用组。CdTe QDs作用于细胞后,采用倒置荧光显微镜分别观察量子点作用下的细胞形态与细胞对量子点的摄取情况;使用alamarBlue检测CdTe QDs对细胞存活率的影响;Hoechst 33342单染法观察CdTe QDs对PC-12细胞核形态的影响;Annexin V-FITC单染检测CdTe QDs对PC-12细胞凋亡的影响。结果:CdTe量子点作用后,低浓度实验组细胞变化不明显,高浓度实验组中的细胞的形态发生改变,引起细胞肿胀变圆,细胞数量减少,存活率显著下降;在染色下可见明显的细胞凋亡。结论:量子点与细胞接触后会通过胞吞途径进入细胞,汇聚于多种细胞器,对细胞器的功能造成损害,进而引发多种途径的细胞凋亡机制并引发细胞死亡。
Objective: To investigate the biological security of CdTe QDs. Methods: CdTe QDs which was made by hydrothermal method and covered by MSA was chosen as the object. The cells were divided into control group and CdTe QDs-treated groups with different concentrations of CdTe QDs. Affected by CdTe QDs. The cell morphology and the fluorescence of CdTe QDs was observed by inverted fluorescence microscope. The cell survival rate was examined by alamarBlue, and cell apoptotic morphology in staining was detected by Hoechst 33342. The influence of CdTe QDs on the apoptosis of PC-12 was examined by Annexin V-FITC single-stain procedure.Results: Affected by CdTe QDs, cells in the experimental group treated with low concentration of CdTe QDs changed not obviously while the morphology of those treated with high concentration obviously changed. In addition, they swelled and became round, the number of which decreased, and the survival rate decreased significantly. It was obvious apoptosis in staining. Conclusions: We consider that quantum point contact with cells, will be uptaken into the cell, together in various organelle, damage the function of the organelle, cause various pathways of apoptosis mechanism and cause cell death at last.
Objective: To investigate the biological security of CdTe QDs. Methods: CdTe QDs which was made by hydrothermal method and covered by MSA was chosen as the object. The cells were divided into control group and CdTe QDs-treated groups with different concentrations of CdTe QDs. Affected by CdTe QDs. The cell morphology and the fluorescence of CdTe QDs was observed by inverted fluorescence microscope. The cell survival rate was examined by alamarBlue, and cell apoptotic morphology in staining was detected by Hoechst 33342. The influence of CdTe QDs on the apoptosis of PC-12 was examined by Annexin V-FITC single-stain procedure.Results: Affected by CdTe QDs, cells in the experimental group treated with low concentration of CdTe QDs changed not obviously while the morphology of those treated with high concentration obviously changed. In addition, they swelled and became round, the number of which decreased, and the survival rate decreased significantly. It was obvious apoptosis in staining. Conclusions: We consider that quantum point contact with cells, will be uptaken into the cell, together in various organelle, damage the function of the organelle, cause various pathways of apoptosis mechanism and cause cell death at last.
引文
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[16]徐进.乙酸铅诱导PC 12细胞凋亡的信号途径研究[D].浙江大学医学院,2008Xu Jin.Study on the signal pathway of lead acetate induced apoptosis of PC 12 cell[D].Medical college of Zhejiang University,2008
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[2]Alivisatos AP.Semiconductor clusters,nanocrystals,and quantum dots[J].Science,1996,271,933-937
[3]罗翀.多晶硅及碲化镉薄膜光伏材料关键制备技术的研究[D].南开大学,2010Luo Chong.Research on polycrystalline silicon and cadmium telluride thin film photovoltaic materials preparation technology[D].Nankai University,2010
[4]翟光美.IV-VI族化合物量子点太阳能电池的制备及其性能研究[D].华中科技大学,2012Zhai Guang-mei.Study on the performance of Science and Technology and preparation of IV-VI compound quantum dot solar cells[D].Huazhong University,2012
[5]赵冬梅,孙立国,王彦杰,等.碲化镉纳米晶的制备及应用[J].化学展,2012,07:1277-1293Zhao Dong-mei,Sun Li-guo,Wang Yan-jie,et al.The preparation and application of CdTe nanocrystals[J].Chemical Exhibition,2012,07:1277-1293
[6]孙晓宁,孙康宁,朱广楠,等.纳米颗粒材料的毒性研究与安全性展望[J].材料导报,2006,20(5):5-7Sun Xiao-ning,Sun Kang-ning,Zhu Guang-nan,et al.Prospect of toxicity and safety of nano particle material[J].Journal of materials,2006,20(5):5-7
[7]许臻,李冬梅,姚亚玲,等.硫脲修饰法制备高发光性能CdTe量子点[J].物理化学学报,2009,06:1201-1206Xu Zhen,Li Dong-mei,Yao Ya-ling,et al.Thiourea modified preparation of luminescent properties of CdTe quantum dots[J].Chinese Journal of Chemical Physics,2009,06:1201-1206
[8]严明.碲化镉量子点对血管内皮细胞的毒性及相关机制研究[D].浙江大学,2011Yan Ming.Study on vascular endothelial cell toxicity and mechanism of CdTe quantum dots[D].Zhejiang University,2011
[9]杨阳,刘宝瑞,钱晓萍.Alamar Blue法用于体外培养细胞活性检测的方法研究[J].现代肿瘤医学,2006,01:6-8Yang Yang,Liu Bao-rui,Qian Xiao-ping.Research methods for detecting cell activity in vitro of Alamar Blue method[J].Modern medical journal,2006,01:6-8
[10]刘锡娟,丁慧荣,张宏.用DAPI和Hoechst33342染色法检测DNA的流式细胞方法探讨[J].北京大学学报(医学版),2010,04:480-484Liu Xi-juan,Ding Hui-rong,Zhang Hong.Discussion on the method for flow cytometry staining was used to detect DNA DAPI and Hoechst33342[J].Journal of Peking University(Medical Sciences),2010,04:480-484
[11]陈大明,金小海,罗志福.放射性Annexin V活体检测细胞凋亡的研究进展[J].原子能科学技术,2004,38(1):35-42Chen Da-ming,Jin Xiao-hai,Luo Zhi-fu.Research progress of apoptosis detection of radioactive Annexin V in vivo[J].Atomic Energy Science and technology,2004,38(1):35-42
[12]朱霖,刘锰沈,锐何.Annexin V在核医学细胞凋亡显像中的应用[J].中华核医学杂志,2004,24(6):379-381Zhu Lin,Liu Meng-shen,Rui He.Annexin V application in nuclear medicine imaging of apoptosis[J].Chinese Journal of nuclear medicine,2004,24(6):379-381
[13]祁本忠,陈大明,杨红伟.细胞凋亡显像剂99Tcm-HYNIC-Annexin V的制备及其生物评价[J].同位素,2006,19(3):140-145Qi Ben-zhong,Chen Da-ming,Yang Hong-wei.biological evaluation of the preparation of cell apoptosis imaging agent 99Tcm-HYNICAnnexin V[J].Isotope,2006,19(3):140-145
[14]刘闽碧.用Excel做t检验与F检验[J].海峡预防医学杂志,2002,05:67-68Liu Min-bi.T test and F test by Excel[J].Journal of Preventive Medicine,2002,05:67-68
[15]张云娇.特异性表面结合肽调控稀土纳米材料自噬和毒性的研究[D].中国科学技术大学,2012Zhang Yun-jiao.Study on peptide regulation of rare earth nanomaterials autophagy and toxicity of combined with specific surface[D].University of Science and Technology of China,2012
[16]徐进.乙酸铅诱导PC 12细胞凋亡的信号途径研究[D].浙江大学医学院,2008Xu Jin.Study on the signal pathway of lead acetate induced apoptosis of PC 12 cell[D].Medical college of Zhejiang University,2008
[17]汤睿,朱正纲.凋亡途径与肿瘤治疗[J].世界华人消化杂志,2005,13(20):2469-2472Tang Rui,Zhu Zheng-gang.Theway of apoptosis and tumor treatment[J].World Journal of Gastroenterology,2005,13(20):2469-2472
[18]李娜,高俊岩,刘敏.细胞凋亡和肿瘤的关系研究进展[J].当代医学,2009(171):13-14Li Na,Gao Jun-yan,Liu Min.the research progress of the relationship betweencell apoptosis andtumor[J].Contemporary Medicine,2009(171):13-14
[19]Evan.G,Littlewood.T.A matter of life and cell death[J].Science,1998,28:1317-1322
[20]R.Kumar,P.E.Herbert,A.N.Warrens.An introduction to death receptors in apoptosis[D].International Journal of Surgery,2005(3):268-277