摘要
卷烟烟气在人体口腔中的变化对吸烟与健康研究至关重要,本文设计建造了一套可实时检测口腔中烟气烟碱浓度的采样分析系统。在该系统中,吸烟机将烟气推送到口腔中,然后利用负压毛细管采样进入四极杆质谱仪实时分析检测烟碱的两种特征峰离子(m/z=84和m/z=162),可实时测量单口烟气中的烟碱成分在口腔中的浓度衰减变化情况。用该装置研究屏息状态下,人口腔中烟气烟碱的浓度变化,结果表明:相对于刚进入口腔时的最高烟碱浓度,2 s时口腔烟气烟碱浓度降低了5%左右,随着停留时间的延长,烟碱的口腔浓度降低率逐渐上升。
Measurement of the dynamic behavior of cigarette smoke components in oral cavity is important for researches on smoking and health. In this study an on-line measurement system for real-time analysis of nicotine concentration in cigarette smoke was established.Cigarette smoke was injected into oral cavity via smoking machine, and then directly introduced into the vacuum chamber through a heated transfer line. Two characteristic fragment ions(m/z = 84 and m/z = 162) of nicotine were monitored with a quadrupole mass spectrometer.The attenuation variation of nicotine concentration in oral cavity can be measured in real time. Nicotine concentration of cigarette smoke in oral cavity at holding breath state was also studied by using this apparatus. Results showed that nicotine concentration of cigarette smoke was the highest when first entering oral cavity, and then declined by about 5% with 2-second holding time. With the increase of holding time in oral cavity, the reduction rate of nicotine concentration showed a rising trend.
引文
[1]Baker R R,Dixon M.The retention of tobacco smoke constituents in the human respiratory tract[J].Inhalation toxicology,2006,18(4):255-294.
[2]Feng S,Kapur S,Sarkar M,et al.Respiratory retention of nicotine and urinary excretion of nicotine and its five major metabolites in adult male smokers[J].Toxicol Lett,2007,173:101-106.
[3]Marco E,Grimalt JO.A rapid method for the chromatographic analysis of volatile organic compounds in exhaled breath of tobacco cigarette and electronic cigarette smokers[J].J Chromatogr A,2015,1410:51-59.
[4]Ingerbrethsen B J.The physical properties of mainstream cigarette smoke and their relationship to deposition in the respiratory tract[M].Extrapolation of dosimetric relationships for inhaled particles and gases,New York:Academic Press,1989.Serban C M.Beitr?ge Zur Tabak Inter.,2007,22(4):290-302.
[5]宁敏,胡永华,徐迎波,等.同步辐射真空紫外光电离质谱法实时分析卷烟主流烟气气相成分.中国烟草学报,2013,19(4):11.NING Min,HU Yonghua,XU Yingbo,et al.Real-time analysis of gas-phase mainstream cigarette smoke by synchrotron radiation VUV single-photon ionization time-of-lfight mass spectrometry[J].Acta Tabacaria Sinica,2013,19(4):11.
[6]Mühlberger F,Streibel T,Wieser J,et al.Single photon ionization time-of-flight mass spectrometry with a pulsed electron beam pumped excimer VUV lamp for on-line gas analysis:setup and first results on cigarette smoke and human breath[J].Analytical Chemistry,2005,77(22):7408-7414.
[7]王健,胡永华,张晓宇,等.光电离质谱法测定卷烟烟气中有害成分的口腔残留[J].烟草科技,2011(8):48-51.WANG Jian,HU Yonghua,ZHANG Xiaoyu,et al.Determination of buccal residues of harmful components from cigarette smoke by photoionization mass spectrometry[J].Tobacco Science&Technology,2011(8):48-51.
[8]Pan Y,Hu Y H,Wang J,et al.Online characterization of isomeric/isobaric components in the gas phase of mainstream cigarette smoke by tunable synchrotron radiation vacuum ultraviolet photoionization time-of-flight mass spectrometry and photoionization efficiency curve simulation[J].Analytical Chemistry,2013,85(24):11993-12001.
[9]王健,胡永华,田振峰,等.真空紫外灯电离源飞行时间质谱仪的研制及应用[J].分析实验室,2016,35,(10),1236-1240.WANG Jian,HU Yonghua,TIAN Zhenfeng,et al.Development of vacuum ultraviolet lamp ionization source time-of-flight mass spectrometry and its applications[J].Chin J Anal Lab,2016,35(10):1236-1240.
[10]Eschner MS,Selmani I,Groger TM,et al.Online Comprehensive Two-Dimensional Characterization of Puff-by-Puff Resolved Cigarette Smoke by Hyphenation of Fast Gas Chromatography to Single-Photon Ionization Time-of-Flight Mass Spectrometry:Quantification of Hazardous Volatile Organic Compounds[J].Anal Chem,2011,83,6619-6627.
[11]周烽,谭国斌,冯艳丽,等.在线飞行时间质谱仪分析香烟烟气[J].分析实验室,2012,31,(10),31-35.ZHOU Feng,TAN Guobing,FENG Yanli,et al.Analysis of old cigarette smoke by utilizing online time-of-flight mass spectrometry[J].Chin J Anal Lab,2012,31(10),31-35.
[12]Streibel T,Mitschke S,Adam T,et al.Time-resolved analysis of the emission of sidestream smoke(SSS)from cigarettes during smoking by photo ionisation/time-of-flight mass spectrometry(PI-TOFMS):towards a better description of environmental tobacco smoke[J].Anal Bioanal Chem,2013,405:7071-7082.
[13]Senneca O,Ciaravolo S and Nunziata A.Composition of the gaseous products of pyrolysis of tobacco under inert and oxidative conditions[J].J Anal Appl Pyrolysis,2007,79(1/2):234-243.