甘草黄酮抗氧化及免疫活性
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摘要
为了研究甘草黄酮的抗氧化及免疫活性,试验通过DPPH自由基、超氧阴离子自由基和羟基自由基的清除率测定甘草总黄酮的抗氧化活性,通过测定不同浓度的甘草总黄酮刺激小鼠脾脏淋巴细胞增殖能力、小鼠脾脏淋巴细胞抗体形成的影响,研究甘草黄酮的免疫调节作用。结果显示,甘草黄酮对DPPH自由基清除率为25%,对超氧阴离子自由基清除率为79.07%,对羟基自由基清除率为29.51%。各试验组小鼠脾脏淋巴细胞增殖能力均显著高于对照组;IL-2水平试验中,各试验组均显著高于对照组,试验3组极显著高于对照组,显著高于1,2组;在IgG抗体水平分析中,甘草黄酮各试验组均极限著高于对照组。说明甘草黄酮具有较好的抗氧化及免疫活性。
To investigate the antioxidant and immunological activities of licorice flavonoids,the antioxidant activity of total flavonoids in licorice was determined by the clearance rates of DPPH radicals,superoxide anion radicals,and hydroxyl radicals,and the spleen of mice was stimulated by total concentrations of flavonoids of licorice.Through measuring lymphocyte proliferation and antibody formation in mouse spleen the immunomodulatory effect of licorice flavonoids was investigated.The results showed that the scavenging rate of DPPH free radicals by licorice flavonoids was 25%,the superoxide anion radical scavenging rate was 79.07%,and the hydroxyl radical scavenging rate was 29.51%.All experimental groups were significantly higher in the spleen lymphocyte proliferation of mice than in the control group;in the IL-2 level,the experimental group 3 was significantly higher than the control group,and there was no significant difference between the experimental groups 1 and 2 and the control group;in the IgG antibody,in each test group was significantly higher than the control group.It shows that licorice flavonoids have good antioxidant and immune activity.
To investigate the antioxidant and immunological activities of licorice flavonoids,the antioxidant activity of total flavonoids in licorice was determined by the clearance rates of DPPH radicals,superoxide anion radicals,and hydroxyl radicals,and the spleen of mice was stimulated by total concentrations of flavonoids of licorice.Through measuring lymphocyte proliferation and antibody formation in mouse spleen the immunomodulatory effect of licorice flavonoids was investigated.The results showed that the scavenging rate of DPPH free radicals by licorice flavonoids was 25%,the superoxide anion radical scavenging rate was 79.07%,and the hydroxyl radical scavenging rate was 29.51%.All experimental groups were significantly higher in the spleen lymphocyte proliferation of mice than in the control group;in the IL-2 level,the experimental group 3 was significantly higher than the control group,and there was no significant difference between the experimental groups 1 and 2 and the control group;in the IgG antibody,in each test group was significantly higher than the control group.It shows that licorice flavonoids have good antioxidant and immune activity.
引文
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[2] 栾祖香,徐子恒,董秀凯,等.HPLC检测甘草颗粒中甘草酸及其抗菌活性[J].中国兽医学报,2018,38(5):974-977.
[3] 于鹏飞,王建平,蒋海强,等.甘草中黄酮类抗氧化成分的提取工艺研究[J].食品与药品,2008(9):25-27.
[4] 戴应和,龙小琴,张娟,等.甘草黄酮的抗炎作用及其机制的研究进展[J].江西中医药,2017(2):68-71.
[5] 崔誉蓉,李德芳,鞠宝,等.四种甘草黄酮类化合物体外抗肿瘤作用研究[J].食品科技,2010(7):88-92.
[6] 何璐,王勇,吕跃东,等.甘草黄酮的提取分离及抗菌活性研究[J].湖北农业科学,2008(2):217-219.
[7] 汪河滨,周忠波,罗锋,等.甘草黄酮提取方法及抗氧化活性研究[J].时珍国医国药,2008(9):2106-2107.
[8] 方鉴.甘草及其有效成分对免疫系统调节作用探究[J].海峡药学,2017(8):28-30.
[9] MARKLUND S,MARKLUND G.Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase[J].Eur J Biochem,1974,47(3):469-474.
[10] CUIN T A,SHABALA S.Compatible solutes reduce ROS-induced potassium efflux in Arabidopsis roots[J].Plant Cell Environ,2007,30(7):875-885.
[11] 官佳懿,张永红,崔德凤,等.绿原酸对小鼠脾脏淋巴细胞体外增殖和分泌功能的影响[J].中国农学通报,2013(29):27-31.
[12] 蒋歆.甘草废弃物中黄酮的提取分离及其抗氧化作用研究[D].甘肃兰州:兰州大学,2011:64.
[13] 张改平.甘草黄酮体外抗氧化活性的研究[D].陕西西安:陕西师范大学,2008:53.
[14] 朱雪峰,谢鲲鹏,霍洪楠,等.甘草提取物的抑菌作用及其对小鼠免疫功能的影响[J].中国微生态学杂志,2013(3):254-257.