东北野生薄荷挥发油成分及抑菌活性研究
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摘要
[目的]研究东北野生薄荷的挥发油化学成分及抑菌活性。[方法]运用水蒸气蒸馏法提取挥发油,采用气相色谱-质谱联用技术(GC-MS)对挥发油化学成分进行分析。运用滤纸片琼脂板扩散法和二倍稀释法,对挥发油的抑菌活性和最小抑菌浓度进行研究。[结果]薄荷挥发油出油率为0.8%,通过运用气相色谱-质谱联用技术(GC-MS)鉴定出东北野生薄荷的挥发油成分有59种。提取出的薄荷挥发油对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌等常用菌种均有抑制作用,且抑菌圈直径分别为10.92、10.25、9.18 mm,最小抑菌浓度分别为1.56、3.13、12.50μL/mL。[结论]用GC-MS法检测出东北野生薄荷的挥发油化学成分较多,且挥发油表现出良好的抑菌活性,具有很大的市场潜力。
[Objective] The research aimed to study the chemical composition and antibacterial activity of the volatile oils from wild Mentha haplocalyx in Northeast China. [Method]GC-MS was employed to analyze the composition of the volatile oil from Mentha haplocalyx extracted.The antibacterial activity and minimum inhibitory concentration of volatile oil were studied by using the paper agar diffusion method and double broth dilution method. [Result]The yield of volatile oil from Mentha haplocalyx was 0.8%. 59 main vo1 atile compounds were identified by the gas chromatography-mass spectrometry. The extracted volatile oil had an inhibitory effect on common strains such as Staphylococcus aureus, Escherichia coli and Bacillus subtilis,and the diameter of the inhibition zone was 10.9,10.25 and 9.18 mm, respectively. The minimum inhibitory concentrations were 1.56, 3.13, 12.50 L/mL, respectively. [Result]Many chemical constituents of the volatile oil from wild Mentha haplocalyx in Northeast China were detected by GC-MS. The volatile oil showed good antibacterial activity,so Mentha haplocalyx Briq may have great market potential.
[Objective] The research aimed to study the chemical composition and antibacterial activity of the volatile oils from wild Mentha haplocalyx in Northeast China. [Method]GC-MS was employed to analyze the composition of the volatile oil from Mentha haplocalyx extracted.The antibacterial activity and minimum inhibitory concentration of volatile oil were studied by using the paper agar diffusion method and double broth dilution method. [Result]The yield of volatile oil from Mentha haplocalyx was 0.8%. 59 main vo1 atile compounds were identified by the gas chromatography-mass spectrometry. The extracted volatile oil had an inhibitory effect on common strains such as Staphylococcus aureus, Escherichia coli and Bacillus subtilis,and the diameter of the inhibition zone was 10.9,10.25 and 9.18 mm, respectively. The minimum inhibitory concentrations were 1.56, 3.13, 12.50 L/mL, respectively. [Result]Many chemical constituents of the volatile oil from wild Mentha haplocalyx in Northeast China were detected by GC-MS. The volatile oil showed good antibacterial activity,so Mentha haplocalyx Briq may have great market potential.
引文
[1] 中国科学院《中国植物志》编辑委会.中国植物志:第66卷第2册 [M].北京:科学出版社,1977.
[2] 朱梅芳,唐宇,郑琴,等.不同提取方式对连翘、荆芥、薄荷挥发油成分及抗菌活性的影响[J].中草药,2018,49(12):2845-2854.
[3] 李岗,余德顺,杨军,等.超临界CO2萃取薄荷挥发油及其抗氧化能力的研究[J].食品科技,2013,38(1):276-279.
[4] 杨倩.薄荷挥发油的化学型分析及抑菌、抗炎活性研究[D].镇江:江苏大学,2017.
[5] 靳有才,庆易薇,郭珍.青海野生薄荷挥发油成分GC-MS分析[J].中国实验方剂学杂志,2013,19(23):143-146.
[6] RAHMAN A,SHANTA Z S,RASHID M A,et al.In vitro antibacterial properties of essential oil and organic extracts of Premna integrifolia Linn[J].Arabian journal of chemistry,2016,9(S1):475-479.
[7] SINGH R,SHUSHNI M A M,BELKHEIR A.Antibacterial and antioxidant activities of Mentha piperita L.[J].Arabian journal of chemistry,2015,8(3):322-328.
[8] 孙亚栋,阿布力米提·阿布都卡德尔.薄荷叶挥发油成分的GC-MS分析[J].新疆大学学报(自然科学版),2017,34(1):61-63.
[9] 田祎穎,姚婷,黄光文,等.湖南永州薄荷挥发油化学成分GC-MS分析[J].山东化工,2018,47(15):96-97.
[10] REDDY D N,AL-RAJAB A J,SHARMA M,et al.Chemical constituents,in vitro antibacterial and antifungal activity of Mentha × Piperita L (peppermint)essential oils[J/OL].Journal of King Saud University-Science,2017[2018-11-20].doi:http://dx.doi.org/10.1016/j.jksus.2017.07.013.
[2] 朱梅芳,唐宇,郑琴,等.不同提取方式对连翘、荆芥、薄荷挥发油成分及抗菌活性的影响[J].中草药,2018,49(12):2845-2854.
[3] 李岗,余德顺,杨军,等.超临界CO2萃取薄荷挥发油及其抗氧化能力的研究[J].食品科技,2013,38(1):276-279.
[4] 杨倩.薄荷挥发油的化学型分析及抑菌、抗炎活性研究[D].镇江:江苏大学,2017.
[5] 靳有才,庆易薇,郭珍.青海野生薄荷挥发油成分GC-MS分析[J].中国实验方剂学杂志,2013,19(23):143-146.
[6] RAHMAN A,SHANTA Z S,RASHID M A,et al.In vitro antibacterial properties of essential oil and organic extracts of Premna integrifolia Linn[J].Arabian journal of chemistry,2016,9(S1):475-479.
[7] SINGH R,SHUSHNI M A M,BELKHEIR A.Antibacterial and antioxidant activities of Mentha piperita L.[J].Arabian journal of chemistry,2015,8(3):322-328.
[8] 孙亚栋,阿布力米提·阿布都卡德尔.薄荷叶挥发油成分的GC-MS分析[J].新疆大学学报(自然科学版),2017,34(1):61-63.
[9] 田祎穎,姚婷,黄光文,等.湖南永州薄荷挥发油化学成分GC-MS分析[J].山东化工,2018,47(15):96-97.
[10] REDDY D N,AL-RAJAB A J,SHARMA M,et al.Chemical constituents,in vitro antibacterial and antifungal activity of Mentha × Piperita L (peppermint)essential oils[J/OL].Journal of King Saud University-Science,2017[2018-11-20].doi:http://dx.doi.org/10.1016/j.jksus.2017.07.013.