慢性鼻窦炎黏膜组织中TLR-2的表达研究
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摘要
目的探讨慢性鼻窦炎黏膜组织中Toll样受体-2(TLR-2)表达变化的临床意义。方法 96例慢性鼻窦炎患者,鼻内镜下采集病变部位黏膜组织和正常鼻黏膜组织作为研究标本,采用免疫组化法检测两组标本中TLR-2的表达情况,比较病变部位黏膜组织和正常鼻黏膜组织的TLR-2表达情况。给予患者常规药物治疗,在治疗后再次采集患者病变部位黏膜组织进行TLR-2免疫组化法检测,比较治疗前后病变部位黏膜组织的TLR-2表达情况。结果患者治疗前病变部位黏膜组织TLR-2阳性表达率为81.25%(78/96),明显高于正常鼻黏膜组织的22.92%(22/96),差异具有统计学意义(χ2=65.447, P=0.000<0.05)。患者治疗后的病变部位黏膜组织TLR-2阳性表达率为30.21%(29/96),低于治疗前的81.25%(78/96),差异具有统计学意义(χ2=50.686, P=0.000<0.05)。结论鼻黏膜组织中TLR-2的阳性表达可能为慢性鼻窦炎发生和进展的危险因素。
Objective To discuss the clinical significance of expression of Toll-like receptor-2(TLR-2) in mucosal tissues of chronic sinusitis. Methods A total of 96 patients with chronic sinusitis, and their mucosal tissues of lesions and normal nasal mucosa were collected under nasal endoscopy as study specimens. The expression of TLR-2 was detected by immunohistochemical method in two groups, and the expression of TLR-2 in the lesion and normal nasal mucosa was compared. All patients received routine drug therapy. After treatment, the mucosal tissues of the lesions were collected again for TLR-2 immunohistochemistry, and the expression of TLR-2 in the mucosa of the lesions before and after treatment was compared. Results The positive expression rate of TLR-2 was 81.25%(78/96) in the mucosal lesions of patients before treatment, which was significantly higher than 22.92%(22/96) in the normal nasal mucosa, and the difference was statistically significant(χ2=65.447, P=0.000<0.05). The positive expression rate of TLR-2 in mucosal tissues of patients after treatment was 30.21%(29/96), which was lower than 81.25%(78/96) before treatment, and the difference was statistically significant(χ2=50.686, P=0.000<0.05). Conclusion The positive expression of TLR-2 in nasal mucosa may be a risk factor for the occurrence and progression of chronic sinusitis.
Objective To discuss the clinical significance of expression of Toll-like receptor-2(TLR-2) in mucosal tissues of chronic sinusitis. Methods A total of 96 patients with chronic sinusitis, and their mucosal tissues of lesions and normal nasal mucosa were collected under nasal endoscopy as study specimens. The expression of TLR-2 was detected by immunohistochemical method in two groups, and the expression of TLR-2 in the lesion and normal nasal mucosa was compared. All patients received routine drug therapy. After treatment, the mucosal tissues of the lesions were collected again for TLR-2 immunohistochemistry, and the expression of TLR-2 in the mucosa of the lesions before and after treatment was compared. Results The positive expression rate of TLR-2 was 81.25%(78/96) in the mucosal lesions of patients before treatment, which was significantly higher than 22.92%(22/96) in the normal nasal mucosa, and the difference was statistically significant(χ2=65.447, P=0.000<0.05). The positive expression rate of TLR-2 in mucosal tissues of patients after treatment was 30.21%(29/96), which was lower than 81.25%(78/96) before treatment, and the difference was statistically significant(χ2=50.686, P=0.000<0.05). Conclusion The positive expression of TLR-2 in nasal mucosa may be a risk factor for the occurrence and progression of chronic sinusitis.
引文
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[8]王金春,张海莲,刘军权,等. Hp感染胃黏膜IL-17、TLR2及PD-1表达及意义.徐州医学院学报, 2017, 37(10):636-641.
[9]高鸿亮,周禾,姚萍.维生素D3对急性溃疡性结肠炎大鼠肠黏膜Toll样受体2和髓样分化蛋白2及CD14表达的影响.中华实用诊断与治疗杂志, 2017, 31(7):649-652.
[10]桃子玺,范挽亭,李娟,等. TLR2和TLR4在人慢性根尖周病组织肥大细胞上的表达.中国病理生理杂志, 2016, 32(3):516-521.
[11]王爱平,孙海波.慢性鼻-鼻窦炎伴鼻息肉患者鼻黏膜组织中YKL-40及TLR4的表达变化及意义.山东医药, 2016, 56(21):88-89.
[12]杨春治.雷公藤内酯醇对大鼠变应性鼻炎TLR2-NF-κB信号通路的干预作用.山西医科大学学报, 2015, 46(7):636-640.
[2]王欣荣,李捷. T-bet、GATA-3、Foxm1在慢性鼻窦炎黏膜组织中的表达及与炎症分子表达的相关性.海南医学院学报,2017, 23(15):2161-2164.
[3]朱政文,曹忠胜,袁辉,等.慢性鼻-鼻窦炎伴鼻息肉筛窦黏膜组织中T-bet和GATA-3的表达.临床耳鼻咽喉头颈外科杂志, 2015, 29(20):1782-1786.
[4]李海春,赵蕊,崔竟飞,等.转录因子T-bet、GATA-3及炎性因子在慢性鼻-鼻窦炎伴鼻息肉中的表达及意义.医学临床研究, 2017, 34(6):1195-1197.
[5]陈布衣,张昌明.慢性鼻窦炎黏膜组织中Eotaxin-3、Foxm1表达量与p38MAPK/NF-κB、炎症因子表达的相关性.海南医学院学报, 2017, 23(13):1874-1876.
[6]赵阳,程良军,孔磊,等.慢性鼻窦炎黏膜组织中TLR-2、TLR-9及IL-22R表达及意义.医学临床研究, 2015, 32(12):2403-2406.
[7]丁楠,李春苗,巴云鹏. PLUNC、TLR2及NF-κB在鼻息肉组织中的表达及意义.临床耳鼻咽喉头颈外科杂志, 2017, 31(2):130-134.
[8]王金春,张海莲,刘军权,等. Hp感染胃黏膜IL-17、TLR2及PD-1表达及意义.徐州医学院学报, 2017, 37(10):636-641.
[9]高鸿亮,周禾,姚萍.维生素D3对急性溃疡性结肠炎大鼠肠黏膜Toll样受体2和髓样分化蛋白2及CD14表达的影响.中华实用诊断与治疗杂志, 2017, 31(7):649-652.
[10]桃子玺,范挽亭,李娟,等. TLR2和TLR4在人慢性根尖周病组织肥大细胞上的表达.中国病理生理杂志, 2016, 32(3):516-521.
[11]王爱平,孙海波.慢性鼻-鼻窦炎伴鼻息肉患者鼻黏膜组织中YKL-40及TLR4的表达变化及意义.山东医药, 2016, 56(21):88-89.
[12]杨春治.雷公藤内酯醇对大鼠变应性鼻炎TLR2-NF-κB信号通路的干预作用.山西医科大学学报, 2015, 46(7):636-640.