影响猪脂肪沉积主要候选基因的研究进展
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摘要
脂肪及脂肪酸是决定猪肉品质的重要因素,尤其是肌内脂肪沉积的含量在肉品质方面起到重要作用。本文综述影响猪脂肪沉积的主要候选基因,初步阐释候选基因影响脂肪沉积以及脂肪代谢的机理,目的是为今后调控猪肌内脂肪沉积提供理论依据。
Fat and fatty acids are important factors in meat quality. Especially,the content of intramuscular fat deposition plays an important role in meat quality. This article summarizes the major candidate genes affecting fat deposition of pigs and explains the mechanism of candidate genes affecting fat deposition and metabolism.The purpose of article is to provide a theoretical basis for the regulation of intramuscular fat deposition in pigs.[Chinese Journal of Animal Nutrition,2019,31(7):2981-2987]
Fat and fatty acids are important factors in meat quality. Especially,the content of intramuscular fat deposition plays an important role in meat quality. This article summarizes the major candidate genes affecting fat deposition of pigs and explains the mechanism of candidate genes affecting fat deposition and metabolism.The purpose of article is to provide a theoretical basis for the regulation of intramuscular fat deposition in pigs.[Chinese Journal of Animal Nutrition,2019,31(7):2981-2987]
引文
[1]潘增祥,陈杰,黄瑞华,等.猪脂肪及肌肉组织中基因表达信息分析[J].遗传学报,2005(3):264-274.
[2] WOOD J D,ENSER M,FISHER A V,et al.Fat deposition,fatty acid composition and meat quality:a review[J].Meat Science,2008,78(4):343-358.
[3]曾勇庆,王根林,魏述东,等.含不同比例莱芜猪血缘杂交猪胴体品质及肉质特性的研究[J].遗传,2005(1):65-69.
[4] HU H M,WANG J Y,ZHU R S,et al.Effect of myosin heavy chain composition of muscles on meat quality in Laiwu pigs and Duroc[J]. Science in China Series C:Life Sciences,2008,51(2):127-132.
[5] LU P,LI D F,YIN J D,et al. Flavour differences of cooked longissimus muscle from Chinese indigenous pig breeds and hybrid pig breed(Duroc×Landrace×Large White)[J]. Food Chemistry,2008,107(4):1529-1537.
[6] GERBENS F,DE KONING D J,HARDERS F L,et al.The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs[J]. Journal of Animal Science,2000,78(3):552-559.
[7] GARDAN D,GONDRET F,LOUVEAU I.Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes[J]. AJP-Endocrinology and Metabolism,2006,291(2):E372-E380.
[8] CHEN J,YANG X J,XIA D,et al. Sterol regulatory element binding transcription factor 1 expression and genetic polymorphism significantly affect intramuscular fat deposition in the longissimus muscle of Erhualian and Sutai pigs[J]. Journal of Animal Science,2008,86(1):57-63.
[9] YUE T,FANG Q,YIN J D,et al. S-adenosylmethionine stimulates fatty acid metabolism-linked gene expression in porcine muscle satellite cells[J].Molecular Biology Reports,2010,37(7):3143-3149.
[10] GOSMAIN Y,LEFAI E,RYSER S,et al.Sterol regulatory element-binding protein-1 mediates the effect of insulin on hexokinaseⅡgene expression in human muscle cells[J].Diabetes,2004,53(2):321-329.
[11] HAUSMAN G J,DODSON MV,AJUWON K,et al.Board-invited review:the biology and regulation of preadipocytes and adipocytes in meat animals[J].Journal of Animal Science,2009,87(4):1218-1246.
[12] EBERLD,CLMENT K,MEYRE D,et al.SREBF-1 gene polymorphisms are associated with obesity and type 2 diabetes in French obese and diabetic cohorts[J].Diabetes,2004,53(8):2153-2157.
[13]李江凌,刘锐,陈晓晖,等.ADD1基因多态性位点与猪肌内脂肪含量、嫩度及背膘厚的关联性研究[J].中国猪业,2013(6):44-45.
[14] DONG X Y,TANG S Q,ZHANG W,et al. GPR39activates proliferation and differentiation of porcine intramuscular preadipocytes through targeting the PI3K/AKT cell signaling pathway[J]. Journal of Receptors and Signal Transduction,2016,36(2):130-138.
[15] PAYNE V A,AU W S,LOWE C E,et al. C/EBP transcription factors regulate SREBP1c gene expression during adipogenesis[J]. Biochemical Journal,2010,425(1):215-223.
[16]盘道兴.不同猪种脂肪细胞分化因子的差异表达研究[D].硕士学位论文.贵阳:贵州大学,2016.
[17]牛丛丛.脂肪和肥胖相关基因(FTO)的转录调控及其功能研究[D].硕士学位论文.武汉:华中农业大学,2012.
[18]张雨林,詹济华,谭洋,等.肥胖相关基因FTO、FNDC5、PRDM16的研究进展[J].生命的化学,2017,37(6):971-979.
[19]周波.猪FTO原核表达及其对猪肌内前体脂肪细胞增殖和分化的影响[D].硕士学位论文.雅安:四川农业大学,2016.
[20] DREYER C,KREY G,KELLER H,et al. Control of the peroxisomalβ-oxidation pathway by a novel family of nuclear hormone receptors[J]. Cell,1992,68(5):879-887.
[21] CHEN X L,ZHOU B,LUO Y L,et al.Tissue distribution of porcine FTO and its effect on porcine intramuscular preadipocytes proliferation and differentiation[J].PLoS One,2016,11(3):e151056.
[22] KLIEWER S A,FORMAN B M,BLUMBERG B,et al.Differential expression and activation of a family of murine peroxisome proliferator-activated receptors[J].Proceedings of the National Academy of Sciences of the United States of America,1994,91(15):7355-7359.
[23] SHER T,YI H F,MCBRIDE O W,et al.cDNA cloning,chromosomal mapping,and functional characterization of the human peroxisome proliferator activated receptor[J].Biochemistry,1993,32(21):5598-5604.
[24] HE K,WANG Q S,WANG Z,et al.Association study between gene polymorphisms in PPAR signaling pathway and porcine meat quality traits[J]. Mammalian Genome,2013,24(7/8):322-331.
[25] MA J J,CHAI J,SHANG Y Y,et al.Swine PPAR-γ2expression upregulated in skeletal muscle of transgenic mice via the swine myozenin-1 gene promoter[J].Transgenic Research,2015,24(3):409-420.
[26] RODRGUEZ A,CATALN V,GMEZ-AMBROSI J,et al. Insulin-and leptin-mediated control of aquaglyceroporins in human adipocytes and hepatocytes is mediated via the PI3K/Akt/mTOR signaling cascade[J].The Journal of Clinical Endocrinology&Metabolism,2011,96(4):E586-E597.
[27] TARDELLI M,CLAUDEL T,BRUSCHI F V,et al.Adiponectin regulates AQP3 via PPARαin human hepatic stellate cells[J]. Biochemical and Biophysical Research Communications,2017,490(1):51-54.
[28] CHEN L,LI Z,ZHANG Q,et al. Silencing of AQP3induces apoptosis of gastric cancer cells via downregulation of glycerol intake and downstream inhibition of lipogenesis and autophagy[J]. OncoTargets and Therapy,2017,10:2791-2804.
[29]杨晶.猪肌内脂肪差异性沉积关键基因鉴定及AQP3的功能研究[D].硕士学位论文.杨凌:西北农林科技大学,2018.
[30] MADEIRA A,MOURA T F,SOVERAL G. Aquaglyceroporins:implications in adipose biology and obesity[J]. Cellular and Molecular Life Sciences,2015,72(4):759-771.
[31] BARB C R,HAUSMAN G J,HOUSEKNECHT K L.Biology of leptin in the pig[J]. Domestic Animal Endocrinology,2001,21(4):297-317.
[32]李永能,黄英,杨明华,等.瘦素(leptin)对猪脂肪细胞FTO基因mRNA表达的影响[J].云南农业大学学报,2014,29(5):666-671.
[33]黄艳娜,何少婧,李莉,等.陆川猪瘦素基因的克隆及序列分析[J].黑龙江畜牧兽医,2015(21):92-95.
[34] PRUSZYNSKA-OSZMALEK E,KOLODZIEJSKI P A,KACZMAREK P,et al. Orexin A but not orexin B regulates lipid metabolism and leptin secretion in isolated porcine adipocytes[J]. Domestic Animal Endocrinology,2018,63:59-68.
[35]朱耀武,陈宇光,罗佳捷.重组猪脂联素对沙子岭猪屠宰性能的影响[J].湖南饲料,2015(4):32-33.
[36]程晓芳.白藜芦醇通过脂联素信号通路调控肌纤维类型转化的研究[D].硕士学位论文.南宁:广西大学,2017.
[37]杨雪梅,顾以韧,陶璇,等.不同猪种脂联素和胰岛素含量与肌内脂肪含量关系研究[J].养猪,2015(4):60-62.
[38] ALBUQUERQUE A,NEVES J A,REDONDEIRO M,et al. Long term betaine supplementation regulates genes involved in lipid and cholesterol metabolism of two muscles from an obese pig breed[J]. Meat Science,2017,124:25-33.
[39]崔波,耿忠诚,潘兴玲,等.甜菜碱与蛋氨酸螯合铬对三江白猪血清生化指标及皮下脂肪组织脂肪酸合成酶基因mRNA表达的影响[J].中国生物制品学杂志,2011,24(5):554-557.
[40] RENAVILLE B,BACCIU N,LANZONI M,et al.Association of single nucleotide polymorphisms in fat metabolism candidate genes with fatty acid profiles of muscle and subcutaneous fat in heavy pigs[J]. Meat Science,2018,139:220-227.
[41]娄鹏博.加权基因共表达网络分析(WGCNA)筛选猪卵母细胞脂肪沉积相关基因[D].硕士学位论文.雅安:四川农业大学,2016.
[42] LI W,LI B,LV J Q,et al. Choline supplementation improves the lipid metabolism of intrauterine-growthrestricted pigs[J]. Asian-Australasian Journal of Animal Sciences,2018,31(5):686-695.
[43] JIAO A R,DIAO H,YU B,et al.Oral administration of short chain fatty acids could attenuate fat deposition of pigs[J].PLoS One,2018,13(5):e196867.
[44] LAFONTAN M,LANGIN D.Lipolysis and lipid mobilization in human adipose tissue[J].Progress in Lipid Research,2009,48(5):275-297.
[45]张雄.从江香猪肌内脂肪预测模型构建及LPL基因相关研究[D].硕士学位论文.贵阳:贵州大学,2017.
[46] ZHANG C,LUO J Q,YU B,et al.Effects of resveratrol on lipid metabolism in muscle and adipose tissues:a reevaluation in a pig model[J]. Journal of Functional Foods,2015,14:590-595.
[47] DUAN Y H,ZHANG L Y,LI F N,et al.β-hydroxy-β-methylbutyrate modulates lipid metabolism in adipose tissues of growing pigs[J]. Food&Function,2018,9(9):4836-4846.
[48] PUGLIESE G,IACOBINI C,PESCE C M,et al. Galectin-3:an emerging all-out player in metabolic disorders and their complications[J]. Glycobiology,2015,25(2):136-150.
[49] KIWAKI K,NOVAK C M,HSU D K,et al.Galectin-3 stimulates preadipocyte proliferation and is up-regulated in growing adipose tissue[J]. Obesity,2007,15(1):32-39.
[50] BAEK J H,KIMS J,KANG H G,et al.Galectin-3 activates PPARγand supports white adipose tissue formation and high-fat diet-induced obesity[J]. Endocrinology,2015,156(1):147-156.
[51]刘美娟.Galectin-3基因的表达规律及其在脂肪代谢中的调控作用[D].硕士学位论文.临汾:山西师范大学,2017.
[52] GAO X G,BALAN V,TAI G H,et al.Galectin-3 induces cell migration via a calcium-sensitive MAPK/ERK1/2 pathway[J]. Oncotarget,2014,5(8):2077-2084.
[53] FUNASAKA T,RAZ A,NANGIA-MAKKER P. Nuclear transport of galectin-3 and its therapeutic implications[J]. Seminars in Cancer Biology,2014,27:30-38.
[2] WOOD J D,ENSER M,FISHER A V,et al.Fat deposition,fatty acid composition and meat quality:a review[J].Meat Science,2008,78(4):343-358.
[3]曾勇庆,王根林,魏述东,等.含不同比例莱芜猪血缘杂交猪胴体品质及肉质特性的研究[J].遗传,2005(1):65-69.
[4] HU H M,WANG J Y,ZHU R S,et al.Effect of myosin heavy chain composition of muscles on meat quality in Laiwu pigs and Duroc[J]. Science in China Series C:Life Sciences,2008,51(2):127-132.
[5] LU P,LI D F,YIN J D,et al. Flavour differences of cooked longissimus muscle from Chinese indigenous pig breeds and hybrid pig breed(Duroc×Landrace×Large White)[J]. Food Chemistry,2008,107(4):1529-1537.
[6] GERBENS F,DE KONING D J,HARDERS F L,et al.The effect of adipocyte and heart fatty acid-binding protein genes on intramuscular fat and backfat content in Meishan crossbred pigs[J]. Journal of Animal Science,2000,78(3):552-559.
[7] GARDAN D,GONDRET F,LOUVEAU I.Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes[J]. AJP-Endocrinology and Metabolism,2006,291(2):E372-E380.
[8] CHEN J,YANG X J,XIA D,et al. Sterol regulatory element binding transcription factor 1 expression and genetic polymorphism significantly affect intramuscular fat deposition in the longissimus muscle of Erhualian and Sutai pigs[J]. Journal of Animal Science,2008,86(1):57-63.
[9] YUE T,FANG Q,YIN J D,et al. S-adenosylmethionine stimulates fatty acid metabolism-linked gene expression in porcine muscle satellite cells[J].Molecular Biology Reports,2010,37(7):3143-3149.
[10] GOSMAIN Y,LEFAI E,RYSER S,et al.Sterol regulatory element-binding protein-1 mediates the effect of insulin on hexokinaseⅡgene expression in human muscle cells[J].Diabetes,2004,53(2):321-329.
[11] HAUSMAN G J,DODSON MV,AJUWON K,et al.Board-invited review:the biology and regulation of preadipocytes and adipocytes in meat animals[J].Journal of Animal Science,2009,87(4):1218-1246.
[12] EBERLD,CLMENT K,MEYRE D,et al.SREBF-1 gene polymorphisms are associated with obesity and type 2 diabetes in French obese and diabetic cohorts[J].Diabetes,2004,53(8):2153-2157.
[13]李江凌,刘锐,陈晓晖,等.ADD1基因多态性位点与猪肌内脂肪含量、嫩度及背膘厚的关联性研究[J].中国猪业,2013(6):44-45.
[14] DONG X Y,TANG S Q,ZHANG W,et al. GPR39activates proliferation and differentiation of porcine intramuscular preadipocytes through targeting the PI3K/AKT cell signaling pathway[J]. Journal of Receptors and Signal Transduction,2016,36(2):130-138.
[15] PAYNE V A,AU W S,LOWE C E,et al. C/EBP transcription factors regulate SREBP1c gene expression during adipogenesis[J]. Biochemical Journal,2010,425(1):215-223.
[16]盘道兴.不同猪种脂肪细胞分化因子的差异表达研究[D].硕士学位论文.贵阳:贵州大学,2016.
[17]牛丛丛.脂肪和肥胖相关基因(FTO)的转录调控及其功能研究[D].硕士学位论文.武汉:华中农业大学,2012.
[18]张雨林,詹济华,谭洋,等.肥胖相关基因FTO、FNDC5、PRDM16的研究进展[J].生命的化学,2017,37(6):971-979.
[19]周波.猪FTO原核表达及其对猪肌内前体脂肪细胞增殖和分化的影响[D].硕士学位论文.雅安:四川农业大学,2016.
[20] DREYER C,KREY G,KELLER H,et al. Control of the peroxisomalβ-oxidation pathway by a novel family of nuclear hormone receptors[J]. Cell,1992,68(5):879-887.
[21] CHEN X L,ZHOU B,LUO Y L,et al.Tissue distribution of porcine FTO and its effect on porcine intramuscular preadipocytes proliferation and differentiation[J].PLoS One,2016,11(3):e151056.
[22] KLIEWER S A,FORMAN B M,BLUMBERG B,et al.Differential expression and activation of a family of murine peroxisome proliferator-activated receptors[J].Proceedings of the National Academy of Sciences of the United States of America,1994,91(15):7355-7359.
[23] SHER T,YI H F,MCBRIDE O W,et al.cDNA cloning,chromosomal mapping,and functional characterization of the human peroxisome proliferator activated receptor[J].Biochemistry,1993,32(21):5598-5604.
[24] HE K,WANG Q S,WANG Z,et al.Association study between gene polymorphisms in PPAR signaling pathway and porcine meat quality traits[J]. Mammalian Genome,2013,24(7/8):322-331.
[25] MA J J,CHAI J,SHANG Y Y,et al.Swine PPAR-γ2expression upregulated in skeletal muscle of transgenic mice via the swine myozenin-1 gene promoter[J].Transgenic Research,2015,24(3):409-420.
[26] RODRGUEZ A,CATALN V,GMEZ-AMBROSI J,et al. Insulin-and leptin-mediated control of aquaglyceroporins in human adipocytes and hepatocytes is mediated via the PI3K/Akt/mTOR signaling cascade[J].The Journal of Clinical Endocrinology&Metabolism,2011,96(4):E586-E597.
[27] TARDELLI M,CLAUDEL T,BRUSCHI F V,et al.Adiponectin regulates AQP3 via PPARαin human hepatic stellate cells[J]. Biochemical and Biophysical Research Communications,2017,490(1):51-54.
[28] CHEN L,LI Z,ZHANG Q,et al. Silencing of AQP3induces apoptosis of gastric cancer cells via downregulation of glycerol intake and downstream inhibition of lipogenesis and autophagy[J]. OncoTargets and Therapy,2017,10:2791-2804.
[29]杨晶.猪肌内脂肪差异性沉积关键基因鉴定及AQP3的功能研究[D].硕士学位论文.杨凌:西北农林科技大学,2018.
[30] MADEIRA A,MOURA T F,SOVERAL G. Aquaglyceroporins:implications in adipose biology and obesity[J]. Cellular and Molecular Life Sciences,2015,72(4):759-771.
[31] BARB C R,HAUSMAN G J,HOUSEKNECHT K L.Biology of leptin in the pig[J]. Domestic Animal Endocrinology,2001,21(4):297-317.
[32]李永能,黄英,杨明华,等.瘦素(leptin)对猪脂肪细胞FTO基因mRNA表达的影响[J].云南农业大学学报,2014,29(5):666-671.
[33]黄艳娜,何少婧,李莉,等.陆川猪瘦素基因的克隆及序列分析[J].黑龙江畜牧兽医,2015(21):92-95.
[34] PRUSZYNSKA-OSZMALEK E,KOLODZIEJSKI P A,KACZMAREK P,et al. Orexin A but not orexin B regulates lipid metabolism and leptin secretion in isolated porcine adipocytes[J]. Domestic Animal Endocrinology,2018,63:59-68.
[35]朱耀武,陈宇光,罗佳捷.重组猪脂联素对沙子岭猪屠宰性能的影响[J].湖南饲料,2015(4):32-33.
[36]程晓芳.白藜芦醇通过脂联素信号通路调控肌纤维类型转化的研究[D].硕士学位论文.南宁:广西大学,2017.
[37]杨雪梅,顾以韧,陶璇,等.不同猪种脂联素和胰岛素含量与肌内脂肪含量关系研究[J].养猪,2015(4):60-62.
[38] ALBUQUERQUE A,NEVES J A,REDONDEIRO M,et al. Long term betaine supplementation regulates genes involved in lipid and cholesterol metabolism of two muscles from an obese pig breed[J]. Meat Science,2017,124:25-33.
[39]崔波,耿忠诚,潘兴玲,等.甜菜碱与蛋氨酸螯合铬对三江白猪血清生化指标及皮下脂肪组织脂肪酸合成酶基因mRNA表达的影响[J].中国生物制品学杂志,2011,24(5):554-557.
[40] RENAVILLE B,BACCIU N,LANZONI M,et al.Association of single nucleotide polymorphisms in fat metabolism candidate genes with fatty acid profiles of muscle and subcutaneous fat in heavy pigs[J]. Meat Science,2018,139:220-227.
[41]娄鹏博.加权基因共表达网络分析(WGCNA)筛选猪卵母细胞脂肪沉积相关基因[D].硕士学位论文.雅安:四川农业大学,2016.
[42] LI W,LI B,LV J Q,et al. Choline supplementation improves the lipid metabolism of intrauterine-growthrestricted pigs[J]. Asian-Australasian Journal of Animal Sciences,2018,31(5):686-695.
[43] JIAO A R,DIAO H,YU B,et al.Oral administration of short chain fatty acids could attenuate fat deposition of pigs[J].PLoS One,2018,13(5):e196867.
[44] LAFONTAN M,LANGIN D.Lipolysis and lipid mobilization in human adipose tissue[J].Progress in Lipid Research,2009,48(5):275-297.
[45]张雄.从江香猪肌内脂肪预测模型构建及LPL基因相关研究[D].硕士学位论文.贵阳:贵州大学,2017.
[46] ZHANG C,LUO J Q,YU B,et al.Effects of resveratrol on lipid metabolism in muscle and adipose tissues:a reevaluation in a pig model[J]. Journal of Functional Foods,2015,14:590-595.
[47] DUAN Y H,ZHANG L Y,LI F N,et al.β-hydroxy-β-methylbutyrate modulates lipid metabolism in adipose tissues of growing pigs[J]. Food&Function,2018,9(9):4836-4846.
[48] PUGLIESE G,IACOBINI C,PESCE C M,et al. Galectin-3:an emerging all-out player in metabolic disorders and their complications[J]. Glycobiology,2015,25(2):136-150.
[49] KIWAKI K,NOVAK C M,HSU D K,et al.Galectin-3 stimulates preadipocyte proliferation and is up-regulated in growing adipose tissue[J]. Obesity,2007,15(1):32-39.
[50] BAEK J H,KIMS J,KANG H G,et al.Galectin-3 activates PPARγand supports white adipose tissue formation and high-fat diet-induced obesity[J]. Endocrinology,2015,156(1):147-156.
[51]刘美娟.Galectin-3基因的表达规律及其在脂肪代谢中的调控作用[D].硕士学位论文.临汾:山西师范大学,2017.
[52] GAO X G,BALAN V,TAI G H,et al.Galectin-3 induces cell migration via a calcium-sensitive MAPK/ERK1/2 pathway[J]. Oncotarget,2014,5(8):2077-2084.
[53] FUNASAKA T,RAZ A,NANGIA-MAKKER P. Nuclear transport of galectin-3 and its therapeutic implications[J]. Seminars in Cancer Biology,2014,27:30-38.