水产动物肠道微生物的研究方法
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摘要
水产动物肠道微生物在保持肠道健康、促使肠道发育、抵御病原侵入、改善体内能量吸收和脂肪代谢过程中起到了非常重要的作用。本文深入探讨了肠道微生物的影响因子和研究方法,以期为肠道微生物对水产动物的影响,及其在水产养殖生产中的应用提供全面的理论知识和应用信息。
Intestinal microflora in aquatic animal plays a key role in maintaining intestinal health, promoting intestinal development, resisting pathogen invasion, regulating the body's energy absorption and the process of fat metabolism. This paper reviewed the influence factors of gut microbes and its research methods, in order to reveal the effect of gut microbes on aquatic animal, and provide comprehensive theoretical knowledge and information of the application in aquaculture production.
Intestinal microflora in aquatic animal plays a key role in maintaining intestinal health, promoting intestinal development, resisting pathogen invasion, regulating the body's energy absorption and the process of fat metabolism. This paper reviewed the influence factors of gut microbes and its research methods, in order to reveal the effect of gut microbes on aquatic animal, and provide comprehensive theoretical knowledge and information of the application in aquaculture production.
引文
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[2]Verner-Jeffreys D W, Shields R J, Bricknell I R, et al. Changes in the gut-associated microflora during the development of Atlantic halibut(Hippoglossus hippoglossus L.)larvae in three British hatcheries[J].Aquaculture, 2003,219(1):21-42.
[3]Al-Harbi A H, Uddin M N. Seasonal variation in the intestinal bacterial flora of hybrid tilapia(Oreo chromisniloticus×Oreochromisaureus)cultured in earthen ponds in Saudi 1Arabia[J]. Aquaculture,2004, 229(1):37-44.
[4]Leamaster B R, Walsh W A, Brock J A, et al. Cold stress-induced changes in the aerobic heterotrophic gastrointestinal tract bacterial flora of red hybrid tilapia[J]. Journal of Fish Biology, 1997, 50(4):770–780.
[5]Ringφ, Sperstad S, Myklebust R, et al. Charac terisation of the microbiota associated with intestine of Atlantic cod(Gadusmorhua L.):The effect of fish meal,standard soybean meal and a bioprocessed soybean meal[J]. Aquaculture, 2006, 261(3):829-841.
[6]M uyzer G, De Waal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J]. Applied and environmental microbiology,1993, 59(3):695-700.
[7]Zoetendal E G, Akkermans A D, Vos W M D.Temperature gradient gel electrophoresis analysis of16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria[J].Applied&Environmental Microbiology, 1998, 64(10):3854–3859.
[8]Zhang X, Yan X, Gao P, et al. Optimized sequence retrieval from single bands of TGGE(temperature gradient gel electrophoresis)profiles of the amplified16S rDNA fragments from an activated sludge system[J]. Journal of Microbiological Methods,2005, 60(1):1-11.
[9]Eckburg P B, Bik E M, Bernstein C N, et al.Diversity of the human intestinal microbial flora[J].Science, 2005, 308(5728):1635–1638.
[10]Shen J, Zhang B, Wei G, et al. Molecular profiling of the Clostridium leptum subgroup in human fecal microflora by PCR-denaturing gradient gel electrophoresis and clone library analysis[J]. Applied and Environmental Microbiology, 2006, 72(8):5232-5238.
[11]Hayashi H, Sakamoto M, Kitahara M, et al.Diversity of the Clostridium coccoides group in human fecal microbiota as determined by 16S rRNA gene library[J]. Fems Microbiology Letters,2006, 257(2):202-207.
[12]李彤彤,李爱华.应用高通量测序技术比较三种不同鱼肠道微生物的群落结构[C]. 2013年湖北省暨武汉微生物学会会员代表大会暨学术年会论文摘要集, 2013.
[2]Verner-Jeffreys D W, Shields R J, Bricknell I R, et al. Changes in the gut-associated microflora during the development of Atlantic halibut(Hippoglossus hippoglossus L.)larvae in three British hatcheries[J].Aquaculture, 2003,219(1):21-42.
[3]Al-Harbi A H, Uddin M N. Seasonal variation in the intestinal bacterial flora of hybrid tilapia(Oreo chromisniloticus×Oreochromisaureus)cultured in earthen ponds in Saudi 1Arabia[J]. Aquaculture,2004, 229(1):37-44.
[4]Leamaster B R, Walsh W A, Brock J A, et al. Cold stress-induced changes in the aerobic heterotrophic gastrointestinal tract bacterial flora of red hybrid tilapia[J]. Journal of Fish Biology, 1997, 50(4):770–780.
[5]Ringφ, Sperstad S, Myklebust R, et al. Charac terisation of the microbiota associated with intestine of Atlantic cod(Gadusmorhua L.):The effect of fish meal,standard soybean meal and a bioprocessed soybean meal[J]. Aquaculture, 2006, 261(3):829-841.
[6]M uyzer G, De Waal E C, Uitterlinden A G. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA[J]. Applied and environmental microbiology,1993, 59(3):695-700.
[7]Zoetendal E G, Akkermans A D, Vos W M D.Temperature gradient gel electrophoresis analysis of16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria[J].Applied&Environmental Microbiology, 1998, 64(10):3854–3859.
[8]Zhang X, Yan X, Gao P, et al. Optimized sequence retrieval from single bands of TGGE(temperature gradient gel electrophoresis)profiles of the amplified16S rDNA fragments from an activated sludge system[J]. Journal of Microbiological Methods,2005, 60(1):1-11.
[9]Eckburg P B, Bik E M, Bernstein C N, et al.Diversity of the human intestinal microbial flora[J].Science, 2005, 308(5728):1635–1638.
[10]Shen J, Zhang B, Wei G, et al. Molecular profiling of the Clostridium leptum subgroup in human fecal microflora by PCR-denaturing gradient gel electrophoresis and clone library analysis[J]. Applied and Environmental Microbiology, 2006, 72(8):5232-5238.
[11]Hayashi H, Sakamoto M, Kitahara M, et al.Diversity of the Clostridium coccoides group in human fecal microbiota as determined by 16S rRNA gene library[J]. Fems Microbiology Letters,2006, 257(2):202-207.
[12]李彤彤,李爱华.应用高通量测序技术比较三种不同鱼肠道微生物的群落结构[C]. 2013年湖北省暨武汉微生物学会会员代表大会暨学术年会论文摘要集, 2013.