葫芦巴碱对热应激致小鼠睾丸损伤的保护作用
|
摘要
目的研究葫芦巴碱对热应激致小鼠睾丸损伤的保护作用。方法雄性ICR小鼠120只,随机分为正常对照组、模型组、葫芦巴碱药物干预组(25 mg/kg,50 mg/kg,100 mg/kg),每组24只。各组小鼠给予相应药物灌胃一周后,除正常对照组外,将其余各组小鼠下腹部置于43℃恒温水浴锅中热应激处理15 min,随后药物干预组给予相应剂量葫芦巴碱灌胃给药14 d,正常对照组和模型组给予生理盐水灌胃14 d,并分别于热刺激处理后1,7,10,14 d取材。计算生殖器官指数,精子数,HE染色观察睾丸组织结构变化,并进行比较分析。结果与正常对照组相比,模型组小鼠生殖器官脏器系数、附睾精子数均显著降低(P<0.05),病理组织学显示小鼠睾丸损伤明显。与模型组比较,各剂量药物干预组均能升高小鼠生殖器官脏器系数及附睾精子数量(P<0.05);病理组织学显示各剂量药物干预组均能明显改善热应激诱导的小鼠睾丸损伤。结论葫芦巴碱能够明显改善热应激对小鼠睾丸的损伤程度,具有一定的保护作用。
Objective To study the protective effect of trigonelline on testicular injury induced by heat stress in mice. Methods A total of 120 ICR male mice were randomly divided into a control group, model group, and trigonelline groups(25 mg/kg, 50 mg/kg, 100 mg/kg), with 24 rats in each group. The lower abdomen of the mice in the model and trigonelline groups was placed in a constant temperature water bath at 43℃ for 15 min to induce heat stress, and then the trigonelline group was treated using trigonelline through intragastric gavage for 14 days. The mice in the control group and the model group were treated using saline for 14 days. Animals were sacrificed at 1, 7, 10, and 14 days after heat stimulation. The genital organ index, a sperm count, and HE staining were used to observe changes in testicular tissue structure and for a comparative analysis. Results Compared with the control group, the genital organ coefficient and the number of epididymal sperm in the model group were significantly decreased(P<0.05). A histopathological analysis showed obvious testicular injury in the mice. Trigonelline treatment increased the genital organ coefficient and the number of spermatozoa in the epididymis, compared with the model group(P<0.05). Histopathology showed that trigonelline treatment significantly reduced heat stress-induced injury in the mice. Conclusions Trigonelline can significantly improve the testicular coefficient and reduce heat stress-induced damage in testis tissue in mice, and it has a certain antagonistic effect.
Objective To study the protective effect of trigonelline on testicular injury induced by heat stress in mice. Methods A total of 120 ICR male mice were randomly divided into a control group, model group, and trigonelline groups(25 mg/kg, 50 mg/kg, 100 mg/kg), with 24 rats in each group. The lower abdomen of the mice in the model and trigonelline groups was placed in a constant temperature water bath at 43℃ for 15 min to induce heat stress, and then the trigonelline group was treated using trigonelline through intragastric gavage for 14 days. The mice in the control group and the model group were treated using saline for 14 days. Animals were sacrificed at 1, 7, 10, and 14 days after heat stimulation. The genital organ index, a sperm count, and HE staining were used to observe changes in testicular tissue structure and for a comparative analysis. Results Compared with the control group, the genital organ coefficient and the number of epididymal sperm in the model group were significantly decreased(P<0.05). A histopathological analysis showed obvious testicular injury in the mice. Trigonelline treatment increased the genital organ coefficient and the number of spermatozoa in the epididymis, compared with the model group(P<0.05). Histopathology showed that trigonelline treatment significantly reduced heat stress-induced injury in the mice. Conclusions Trigonelline can significantly improve the testicular coefficient and reduce heat stress-induced damage in testis tissue in mice, and it has a certain antagonistic effect.
引文
[1] Flammang AM,Cifone MA,Erexson GL,et al.Genotoxicity testing of a fenugreek extract[J].Food Chem Toxical,2004,42(11):1769-1775.
[2] 马波,王志军,付滨,等.葫芦巴的研究进展湖南中医杂志[J].湖南中医杂志,2018,8(34):243-245.
[3] Zhou JY Du XH,Zhang Z Trigonelline inhibits inflammation and protects cell to prevent fetal growth restriction during pregnancy in a mouse model of diabetes[J].Pharmacology,2017,100:209-217.
[4] 卢芙蓉,秦铀,沈霖,等.葫芦巴提取物对肾病综合征大鼠肾损伤的保护作用[J].中西医结合研究,2009,6 (1):281-284.
[5] 史广江.枸杞多糖对链脲佐菌素诱导的糖尿病小鼠睾丸功能障碍的改善作用及其机制研究[D].宁夏医科大学,2017.
[6] 范红艳,王艳春,顾饶胜,等.生精障碍动物模型的研究进展[J].吉林医药学院学报,2013,34(2):120-122.
[7] 基鲁坎达丝·戈皮奈特 (Chirukandath Gopinath),瓦桑特·莫厄特 (Vasanthi Mowat).毒理病理学图谱[M].北京:北京科学技术出版社,2017:118-121.
[8] 杜德克,罗娜.医学组织学图谱[M].北京:人民卫生出版社:2013:262.
[9] 丁劲.益肾健脾方干预少弱精子症小鼠模型的实验研究[D].北京中医药大学,2016.
[10] 兰卫,高晓黎,毛新民.葫芦巴药理作用的研究进展[J].西北药学杂志,2008,23(5):335-336.
[11] Altuntas E,Zgz E,Taser F.Some physical properties of fenugreek (Trigonella foenumgraceum L.) seeds[J].J Food Engin,2005,71(1):37-43.
[12] 马文智,王燕蓉,田见晖.热应激处理对精原细胞增值的影响[J].宁夏医科大学学报,2011,33(12):1131-1133.
[13] 丛霞,姜忠玲.热应激对雄鼠生殖器官及精子发生的影响[J].黑龙江动物繁殖,2007,15(6):4-6.
[14] 梁明振,黄桂花,何烽杰,等.热应激对公兔精液品质、睾丸组织形态及抗氧化指标的影响[J].广西农业科学,2010,41(3):263-265.
[15] 郭洪香,蒋静思,刘波,等.热应激对小鼠睾丸组织的影响[J].中国畜牧兽医文摘,2012,4(28):59-60.
[16] 马文智,田洪成,马会明,等.单次热应激处理对小鼠睾丸生精细胞的影响[J].中华男科学杂志,2016,1(22):6-11.
[17] 张晓伟,杨丹.男性避孕方法的研究概况和可行性分析[J].中国新药与临床杂志,2014,33(2):97-104.
[18] Barazani Y,Katz BF,Nagler HM,et al.Lifestyle,environment,and male reproductive health[J].Urol Clinics North Am,2014,41(1):55-56.
[2] 马波,王志军,付滨,等.葫芦巴的研究进展湖南中医杂志[J].湖南中医杂志,2018,8(34):243-245.
[3] Zhou JY Du XH,Zhang Z Trigonelline inhibits inflammation and protects cell to prevent fetal growth restriction during pregnancy in a mouse model of diabetes[J].Pharmacology,2017,100:209-217.
[4] 卢芙蓉,秦铀,沈霖,等.葫芦巴提取物对肾病综合征大鼠肾损伤的保护作用[J].中西医结合研究,2009,6 (1):281-284.
[5] 史广江.枸杞多糖对链脲佐菌素诱导的糖尿病小鼠睾丸功能障碍的改善作用及其机制研究[D].宁夏医科大学,2017.
[6] 范红艳,王艳春,顾饶胜,等.生精障碍动物模型的研究进展[J].吉林医药学院学报,2013,34(2):120-122.
[7] 基鲁坎达丝·戈皮奈特 (Chirukandath Gopinath),瓦桑特·莫厄特 (Vasanthi Mowat).毒理病理学图谱[M].北京:北京科学技术出版社,2017:118-121.
[8] 杜德克,罗娜.医学组织学图谱[M].北京:人民卫生出版社:2013:262.
[9] 丁劲.益肾健脾方干预少弱精子症小鼠模型的实验研究[D].北京中医药大学,2016.
[10] 兰卫,高晓黎,毛新民.葫芦巴药理作用的研究进展[J].西北药学杂志,2008,23(5):335-336.
[11] Altuntas E,Zgz E,Taser F.Some physical properties of fenugreek (Trigonella foenumgraceum L.) seeds[J].J Food Engin,2005,71(1):37-43.
[12] 马文智,王燕蓉,田见晖.热应激处理对精原细胞增值的影响[J].宁夏医科大学学报,2011,33(12):1131-1133.
[13] 丛霞,姜忠玲.热应激对雄鼠生殖器官及精子发生的影响[J].黑龙江动物繁殖,2007,15(6):4-6.
[14] 梁明振,黄桂花,何烽杰,等.热应激对公兔精液品质、睾丸组织形态及抗氧化指标的影响[J].广西农业科学,2010,41(3):263-265.
[15] 郭洪香,蒋静思,刘波,等.热应激对小鼠睾丸组织的影响[J].中国畜牧兽医文摘,2012,4(28):59-60.
[16] 马文智,田洪成,马会明,等.单次热应激处理对小鼠睾丸生精细胞的影响[J].中华男科学杂志,2016,1(22):6-11.
[17] 张晓伟,杨丹.男性避孕方法的研究概况和可行性分析[J].中国新药与临床杂志,2014,33(2):97-104.
[18] Barazani Y,Katz BF,Nagler HM,et al.Lifestyle,environment,and male reproductive health[J].Urol Clinics North Am,2014,41(1):55-56.