金银花中总黄酮和绿原酸加压同步提取的工艺优化
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摘要
本文旨在探索加压辅助同步提取金银花中总黄酮和绿原酸的工艺。在单因素的基础上进行正交试验,考察了甲醇浓度、料液比、提取压力、提取时间和提取温度因素对总黄酮和绿原酸得率的影响。结果表明,提取压力对金银花中两类化学成分的提取有显著影响(p <0.05);优化工艺条件为甲醇浓度60%、料液比1∶20 (g/m L)、提取压力2 MPa、提取时间30 min、提取温度70℃;工艺条件稳定,与超声波辅助提取法相比,提取效率更高。在此试验条件下,总黄酮和绿原酸的得率分别为15.66%和3.89%。本研究为金银花的开发和利用提供了新的理论依据。
This study was to explore the process of simultaneous extracting total flavonoids and chlorogenic acid from Lonicerae japonicae by pressurization assisted.Based on the single factor experiment,orthogonal experiments were carried out to investigate the effects of solvent concentration,liquid ratio,extraction pressure,extraction time and extraction temperature on the extraction rate of total flavonoids and chlorogenic acid.The results showed that the extraction pressure had a significant effect( p < 0.05) on the extraction of two kinds of chemical constituents from Lonicerae japonicae.The optimum process conditions were as follows:Methanol concentration was 60%,solid-liquid ratio was 1 ∶ 20( g/m L),extraction pressure was 2 MPa,extraction time was30 min,extraction temperature was 70 ℃.The process conditions were stable,and the extraction efficiency was higher than that of ultrasonic assisted extraction method.Under the test conditions,the extraction rates of total flavonoids and chlorogenic acids were respectively 15.66% and 3.89%. This study provided a new theoretical basis for the development and utilization of Lonicerae japonicae.
This study was to explore the process of simultaneous extracting total flavonoids and chlorogenic acid from Lonicerae japonicae by pressurization assisted.Based on the single factor experiment,orthogonal experiments were carried out to investigate the effects of solvent concentration,liquid ratio,extraction pressure,extraction time and extraction temperature on the extraction rate of total flavonoids and chlorogenic acid.The results showed that the extraction pressure had a significant effect( p < 0.05) on the extraction of two kinds of chemical constituents from Lonicerae japonicae.The optimum process conditions were as follows:Methanol concentration was 60%,solid-liquid ratio was 1 ∶ 20( g/m L),extraction pressure was 2 MPa,extraction time was30 min,extraction temperature was 70 ℃.The process conditions were stable,and the extraction efficiency was higher than that of ultrasonic assisted extraction method.Under the test conditions,the extraction rates of total flavonoids and chlorogenic acids were respectively 15.66% and 3.89%. This study provided a new theoretical basis for the development and utilization of Lonicerae japonicae.
引文
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[19]宋文斌,代英成,许敏,等.加速溶剂萃取-液相色谱-紫外检测法测定人参中多种人参皂甙含量[J].现代科学仪器,2009(6):104-108.
[20]顾志荣,师富贵.现代新兴的中药溶剂提取技术研究进展[J].安徽农业科学,2013,41(22):9235-9237.
[21]尹立影.加压溶剂法提取红三叶草异黄酮及其单体分离研究[D].哈尔滨:东北农业大学,2013.
[22]李华生,骆航,孙兴力,等.加压同步萃取艾叶挥发油、总黄酮和多糖的工艺研究[J].中国食品添加剂,2016(10):83-89.
[23]陈莉华,肖斌,贺诚志,等.大孔吸附树脂对金银花叶茎藤总黄酮的吸附性能[J].天然产物研究与开发,2013,25:383-387.
[24]龚志华,任国谱,舒青孝,等.大孔吸附树脂分离纯化金银花绿原酸研究[J].分析测试学报,2011,30(1):85-90.
[25]汪秀妹.金银花中黄酮类化合物的提取及其抗氧化活性的研究[D].福州:福建农林大学,2014.
[2]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:221.
[3]蒋玉林.金银花、茎、叶中绿原酸含量分析对比及综合利用研究[D].长沙:中南大学,2014.
[4]Qiao Z.Lonicera macranthoides‘Huayao-Wanshou’[J].Hortscience A publication of the American Society for Horticultural Science,2013,48(8):1062-1064.
[5]王婵,马敬中,袁超.四种植物的乙醇提取液对鸡蛋保鲜效果的比较研究[J].食品科学,2010,31(12):279-283.
[6]龙彬,李周权,董国忠,等.金银花提取物对蛋鸡生产性能、蛋品质、脂质代谢及蛋黄胆固醇含量的影响[J].动物营养学报,2018,30(1):73-82.
[7]尚晓迪,贾磊.绿原酸在水产饲料中的研究进展[J].科学养鱼,2015(7):69.
[8]徐殿红,范庆龙,高晓艳,等.正交设计优化金银花绿原酸煎提工艺[J].亚太传统医药,2015(4):28-29.
[9]贾霖,黄国清,肖军霞.金银花中黄酮类化合物的提取工艺[J].食品研究与开发,2013(9):41-43.
[10]钟玲,徐婷.超临界CO2萃取全银花叶中绿原酸及挥发油成分研究[J].亚太传统医药,2013(2):43-46.
[11]石奇.金银花绿原酸的酶解工艺及抗氧化活性研究[J].应用化工,2015(9):1643-1646.
[12]刘梦星,王涛,马晶军.金银花中黄酮类化合物的微波辅助提取及抗氧化性[J].湖北农业科学,2014(12):2871-2874.
[13]周莲,周意文,唐春红,等.均匀设计法优化超声提取金银花叶中绿原酸工艺[J].中国食品添加剂,2014(2):148-152.
[14]Hu Wen,Guo Ting,Jiang Wen-Jun,et al.Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica[J].Chinese Journal of Natural Medicines,2015(6):445-453.
[15]李杰,和素娜,杨晖,等.金银花中黄酮类化合物的提取及其检测方法的研究进展[J].食品研究与开发,2015,36(17):175-178.
[16]李杰,王学廷,吴银萍,等.金银花中绿原酸提取方法的研究进展[J].安徽农业科学,2014(16):4969-4970,4973.
[17]曹渊,李创举,夏之宁,等.金银花中绿原酸的超声提取工艺优化[J].时珍国医国药,2008,19(12):2857-2858.
[18]Jesada P,Sutawadee C,Motonobu G,et a1.New approach for extraction of charantin from mordica charantia with pressurized liquid extraction[J].Separation and Purification Technology,2007(52):416-422.
[19]宋文斌,代英成,许敏,等.加速溶剂萃取-液相色谱-紫外检测法测定人参中多种人参皂甙含量[J].现代科学仪器,2009(6):104-108.
[20]顾志荣,师富贵.现代新兴的中药溶剂提取技术研究进展[J].安徽农业科学,2013,41(22):9235-9237.
[21]尹立影.加压溶剂法提取红三叶草异黄酮及其单体分离研究[D].哈尔滨:东北农业大学,2013.
[22]李华生,骆航,孙兴力,等.加压同步萃取艾叶挥发油、总黄酮和多糖的工艺研究[J].中国食品添加剂,2016(10):83-89.
[23]陈莉华,肖斌,贺诚志,等.大孔吸附树脂对金银花叶茎藤总黄酮的吸附性能[J].天然产物研究与开发,2013,25:383-387.
[24]龚志华,任国谱,舒青孝,等.大孔吸附树脂分离纯化金银花绿原酸研究[J].分析测试学报,2011,30(1):85-90.
[25]汪秀妹.金银花中黄酮类化合物的提取及其抗氧化活性的研究[D].福州:福建农林大学,2014.