通脉止渴胶囊薄层色谱鉴别
|
摘要
目的建立通脉止渴胶囊中葛根、天花粉、当归、川芎薄层色谱鉴别法,对该制剂质量进行控制。方法按照《中华人民共和国药典》采用薄层色谱法(TLC)对处方中葛根、天花粉、当归、川芎进行色谱鉴别。结果结合对照品鉴别出该药物的特征斑点,空白对照样品无此斑点。结论薄层色谱法操作简便,重现性好,可用于该制剂的质量控制。
Objective To establish a thin layer chromatography method of Radix Puerariae, Radix Trichosanthis, Radix Angelicae Sinensis, Rhizoma Ligustici Chuanxiong to control the quality of the preparations. Methods According to the Pharmacopoeia of the People's Republic of China, chromatographic identification of Radix Puerariae, Radix Trichosanthis, Radix Angelicae Sinensis, Rhizoma Ligustici Chuanxiong in the prescriptions was carried out by TLC. Results The characteristic spots of the drug were identified in combination with the reference substance, and the blank control sample did not have this spot.Conclusion Thin layer chromatography is easy to operate and reproducible, and can be used for quality control of the preparation.
Objective To establish a thin layer chromatography method of Radix Puerariae, Radix Trichosanthis, Radix Angelicae Sinensis, Rhizoma Ligustici Chuanxiong to control the quality of the preparations. Methods According to the Pharmacopoeia of the People's Republic of China, chromatographic identification of Radix Puerariae, Radix Trichosanthis, Radix Angelicae Sinensis, Rhizoma Ligustici Chuanxiong in the prescriptions was carried out by TLC. Results The characteristic spots of the drug were identified in combination with the reference substance, and the blank control sample did not have this spot.Conclusion Thin layer chromatography is easy to operate and reproducible, and can be used for quality control of the preparation.
引文
[1]梁海宁,刘效栓,沈涛.天芪降糖颗粒制备工艺优选[J].西部中医药,2014,27(10):28-30.
[2]中华人民共和国国家药典委员会.中华人民共和国药典一部[M].北京:中国医药科技出版社,2015:8-9.
[3]王秀芹,林彤,江英桥.薄层色谱法标准化操作要点及实例分析[J].中成药,2018,40(7):1655-1659.
[4]邵小玲,赵同峰.葛根素对早期糖尿病肾病的治疗作用[J].临床荟萃,2003,18(18):1030-1032.
[5]冯果,陈娟,刘文,等.天花粉有效成分及药理活性研究进展[J].微量元素与健康研究,2015,32(6):59-62.
[6]仇伟欣.天花粉药理学研究进展[J].中国中医药信息杂志,1996,3(6):11-13.
[7]王莉,李松林,王涛.降血糖植物多糖和糖甙研究进展[J].中药材,2000,23(9):575-579.
[8]丁建营,刘春娟,郭建军,等.天花粉化学成分的药理活性及其提取与检测方法研究进展[J].中国药房,2018,29(13):1859-1864.
[9]李曦,张丽宏,王晓晓,等.当归化学成分及药理作用研究进展[J].中药材,2013,36(6):1023-1028.
[10]He Y,Li Q,Bi K.Simultaneous determination of six active components by a single standard to determine multicomponents combined with fingerprint analysis for the quality control of Rhizoma Chuanxiong[J].J Sep Sci,2015,38(7):1090-1099.
[2]中华人民共和国国家药典委员会.中华人民共和国药典一部[M].北京:中国医药科技出版社,2015:8-9.
[3]王秀芹,林彤,江英桥.薄层色谱法标准化操作要点及实例分析[J].中成药,2018,40(7):1655-1659.
[4]邵小玲,赵同峰.葛根素对早期糖尿病肾病的治疗作用[J].临床荟萃,2003,18(18):1030-1032.
[5]冯果,陈娟,刘文,等.天花粉有效成分及药理活性研究进展[J].微量元素与健康研究,2015,32(6):59-62.
[6]仇伟欣.天花粉药理学研究进展[J].中国中医药信息杂志,1996,3(6):11-13.
[7]王莉,李松林,王涛.降血糖植物多糖和糖甙研究进展[J].中药材,2000,23(9):575-579.
[8]丁建营,刘春娟,郭建军,等.天花粉化学成分的药理活性及其提取与检测方法研究进展[J].中国药房,2018,29(13):1859-1864.
[9]李曦,张丽宏,王晓晓,等.当归化学成分及药理作用研究进展[J].中药材,2013,36(6):1023-1028.
[10]He Y,Li Q,Bi K.Simultaneous determination of six active components by a single standard to determine multicomponents combined with fingerprint analysis for the quality control of Rhizoma Chuanxiong[J].J Sep Sci,2015,38(7):1090-1099.