啤酒灌胃对糖尿病大鼠血糖代谢功能影响的实验研究
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摘要
目的探讨饮用啤酒对2型糖尿病(DM)大鼠血糖代谢功能的影响。方法 72只6月龄SPF级雄性Wistar大鼠成功建立DM大鼠模型,随机分为模型组(等量蒸馏水灌胃)、低剂量组(0.5 g·kg~(-1)·d~(-1)啤酒灌胃)、中剂量组(2.5 g·kg~(-1)·d~(-1)啤酒灌胃)、高剂量组(5 g·kg~(-1)·d~(-1)啤酒灌胃)各18只,另选18只正常大鼠作为正常组(等量蒸馏水灌胃),灌胃时间均为8周。末次灌胃后取尾静脉血测量不同时间点各组大鼠血糖、胰岛素、胰岛素敏感指数(ISI)、胰高血糖素样肽-1(GLP-1)。结果灌胃前(0 min)时,模型组、低剂量组、中剂量组、高剂量组的血糖、胰岛素、GLP-1水平显著的高于正常组(P<0.05);末次灌胃后(30 min、60 min、90 min、120 min、180 min)时,中剂量组、高剂量组的血糖、胰岛素水平高于正常组、模型组和低剂量组(P<0.05),GLP-1水平显著低于模型组和低剂量组(P<0.05);低剂量组、模型组的血糖、胰岛素、GLP-1水平在末次灌胃后(30 min、60 min、90 min、120 min、180 min、240 min)时比较差异均无统计学意义(P>0.05);灌胃前(0 min)时,模型组、低剂量组、中剂量组、高剂量组的ISI测定值显著低于正常组(P<0.05);末次灌胃后(30 min、60 min、90 min、120 min、180 min、240 min)时,中剂量组、高剂量组的ISI水平低于正常组、模型组和低剂量组(P<0.05);低剂量组、模型组的ISI水平在末次灌胃后(30 min、60 min、90 min、120min、180 min、240 min)时比较差异均无统计学意义(P>0.05)。结论少量啤酒对DM大鼠血糖代谢影响不显著,增加饮酒剂量会降低大鼠胰岛素敏感度及GLP-1水平,进一步升高血糖水平。
Objective To investigate the effects of beer on glucose metabolism in type 2 diabetes mellitus( DM) rats. Methods 72 SPF male Wistar rats of 6 months which were successfully established DM rat model were randomly divided into model group( distilled water gavage),low dose group( 0. 5 g· kg~(-1)·d~(-1) beer gavage),middle dose group( 2. 5 g· kg~(-1)·d~(-1) beer gavage),high dose group( 5 g· kg~(-1)·d~(-1) beer gavage). 18 rats in each group and another 18 rats were in normal control group( distilled water gavage). The intragastric administration lasted for 8 weeks. The blood glucose,insulin,insulin sensitivity index( ISI) and glucagon like peptide-1( GLP-1) were measured at different time points after the last intragastric administration. Results Before gavage( 0 min),blood glucose,insulin and GLP-1 levels of model group,low dose group,middle dose group and high dose group were significantly higher than that of normal group( P < 0. 05). Blood glucose and insulin levels of middle dose group and high dose group in last intragastric administration( 30 min,60 min,90 min,120 min,180 min) were higher than those of normal group,model group and low dose group( P < 0. 05). GLP-1 of model group and low dose group were significantly lower than those of low dose group,model group( P < 0. 05). There was no statistically significant difference between blood glucose,insulin levels,GLP-1 after intragastric administration( 30 min,60 min,90 min,120 min,180 min,240 min) in low dose group and model group( P > 0. 05). ISI value of model group,low dose group,middle dose group and high dose group before gavage( 0 min) were significantly lower than those of normal group( P < 0. 05). The ISI of middle and high dose group after intragastric administration( 30 min,60 min,90 min,120 min,180 min,240 min) were lower than those of normal group,model group and low dose group( P < 0. 05). There was no significant difference of ISI in low dose group,model group after intragastric administration( 30 min,60 min,90 min,120 min,180 min,240 min)( P > 0. 05). Conclusion A small amount of beer has no significant influence on blood glucose metabolism in DM rats,and increased drinking dose can decrease insulin sensitivity and GLP-1 levels,and further increase blood glucose levels.
Objective To investigate the effects of beer on glucose metabolism in type 2 diabetes mellitus( DM) rats. Methods 72 SPF male Wistar rats of 6 months which were successfully established DM rat model were randomly divided into model group( distilled water gavage),low dose group( 0. 5 g· kg~(-1)·d~(-1) beer gavage),middle dose group( 2. 5 g· kg~(-1)·d~(-1) beer gavage),high dose group( 5 g· kg~(-1)·d~(-1) beer gavage). 18 rats in each group and another 18 rats were in normal control group( distilled water gavage). The intragastric administration lasted for 8 weeks. The blood glucose,insulin,insulin sensitivity index( ISI) and glucagon like peptide-1( GLP-1) were measured at different time points after the last intragastric administration. Results Before gavage( 0 min),blood glucose,insulin and GLP-1 levels of model group,low dose group,middle dose group and high dose group were significantly higher than that of normal group( P < 0. 05). Blood glucose and insulin levels of middle dose group and high dose group in last intragastric administration( 30 min,60 min,90 min,120 min,180 min) were higher than those of normal group,model group and low dose group( P < 0. 05). GLP-1 of model group and low dose group were significantly lower than those of low dose group,model group( P < 0. 05). There was no statistically significant difference between blood glucose,insulin levels,GLP-1 after intragastric administration( 30 min,60 min,90 min,120 min,180 min,240 min) in low dose group and model group( P > 0. 05). ISI value of model group,low dose group,middle dose group and high dose group before gavage( 0 min) were significantly lower than those of normal group( P < 0. 05). The ISI of middle and high dose group after intragastric administration( 30 min,60 min,90 min,120 min,180 min,240 min) were lower than those of normal group,model group and low dose group( P < 0. 05). There was no significant difference of ISI in low dose group,model group after intragastric administration( 30 min,60 min,90 min,120 min,180 min,240 min)( P > 0. 05). Conclusion A small amount of beer has no significant influence on blood glucose metabolism in DM rats,and increased drinking dose can decrease insulin sensitivity and GLP-1 levels,and further increase blood glucose levels.
引文
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[12]王志江,冯立刚,刘华,等.急性酒精中毒不同类型所致血糖紊乱的临床分析[J].宁夏医学杂志,2012,34(6):545-546.
[13]闫丽辉,王少真,付亚坤,等.非酒精性脂肪性肝病对糖尿病患者胰岛功能的影响[J].中华内科杂志,2015,54(3):197-200.
[14]车晓礼.酒精摄入量与糖尿病的关系研究[D].泸州:西南医科大学,2015.
[2]胡仁明,谢颖,鹿斌,等.2型糖尿病患者高发"代谢性炎症综合征"[J].中华内分泌代谢杂志,2016,32(1):27-32.
[3]韩丽萍,孙蓓,李春君,等.雷公藤多苷对糖尿病大鼠肝脏Toll样受体4介导免疫炎症信号通路的干预研究[J].中国糖尿病杂志,2016,8(3):152-156.
[4]衡先培,杨柳清,李亮.基于代谢病肝病传脾探索早期干预2型糖尿病的新思路[J].中国中西医结合杂志,2015,35(6):746-751.
[5]卢旭亚,李品川,张朝晖.快速木乃伊化策略治疗糖尿病足临床研究[J].中国实用外科杂志,2015,35(2):198-201.
[6]司晓晨,尚文斌,卞慧敏,等.链脲佐菌素加高脂膳食诱导2型糖尿病大鼠模型[J].安徽中医临床杂志,2003,15(5):383-385.
[7]马晓英,陈玲.2型糖尿病合并非酒精性脂肪性肝病患者胰岛功能异常与空腹血糖的关系[J].临床内科杂志,2017,34(5):343-344.
[8]汪红平,夏娟,喻明.艾塞那肽单药治疗初发肥胖2型糖尿病伴肝功能受损1例[J].中国现代医学杂志,2016,26(22):140-142.
[9]俞东宁,陈萍,王爽,等.龙井茶多糖对ALX糖尿病模型小鼠氧化应激的影响[J].中国食品学报,2016,16(4):30-34.
[10]延华,鲁晓岚,高艳琼,等.西北地区脂肪性肝病的流行病学调查研究[J].中华肝脏病杂志,2015,23(8):622-627.
[11]王芬,吴鹏程,陈蕊,等.酒精性脑桥中央髓鞘溶解症1例[J].广东医学,2016,37(19):3000.
[12]王志江,冯立刚,刘华,等.急性酒精中毒不同类型所致血糖紊乱的临床分析[J].宁夏医学杂志,2012,34(6):545-546.
[13]闫丽辉,王少真,付亚坤,等.非酒精性脂肪性肝病对糖尿病患者胰岛功能的影响[J].中华内科杂志,2015,54(3):197-200.
[14]车晓礼.酒精摄入量与糖尿病的关系研究[D].泸州:西南医科大学,2015.