Box-Behnken效应面法优化牛大力总黄酮提取工艺
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摘要
目的:优化牛大力总黄酮提取工艺。方法:采用超声提取法,以总黄酮提取率为评价指标,以乙醇浓度、乙醇体积、提取时间为考察因素,利用Box-Behnken效应面法优化提取工艺。结果:最佳工艺为乙醇浓度81.07%、乙醇体积21.63倍、超声提取2次、每次52.51 min,温度60℃。在此条件下,牛大力总黄酮提取率达3.81 mg/g。结论:Box-Behnken效应面法优选的提取工艺稳定可行,实验值与预测值相吻合。
Objective To optimize extraction technology of totals flavonoids from Radix Millettia speciosa. Method On the basis of single factor experiment, ultrasonic extraction method was adopted. Ethanol concentration, solvent volume and extraction time were used to examine the yield of total flavonoids by Box-Behnken response surface methodology. Result The optimum conditions of extraction process consisted of 81.07% ethanol for 21.63 times, 52.51 min extraction time, and twice ultrasonic with 60 ℃, while the extraction rate of totals flavonoids was 3.81 mg/g. Conclusion This optimized technology was stable and feasible by Box-Behnken response surface methodology, which the predictive value and the experimental observation value was fitted.
Objective To optimize extraction technology of totals flavonoids from Radix Millettia speciosa. Method On the basis of single factor experiment, ultrasonic extraction method was adopted. Ethanol concentration, solvent volume and extraction time were used to examine the yield of total flavonoids by Box-Behnken response surface methodology. Result The optimum conditions of extraction process consisted of 81.07% ethanol for 21.63 times, 52.51 min extraction time, and twice ultrasonic with 60 ℃, while the extraction rate of totals flavonoids was 3.81 mg/g. Conclusion This optimized technology was stable and feasible by Box-Behnken response surface methodology, which the predictive value and the experimental observation value was fitted.
引文
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[8]周添浓,刘丹丹,唐立海,等.牛大力对四氯化碳及酒精所致小鼠急性肝损伤的保护作用[J].时珍国医国药,2009,20(10):2585-2587.
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[11]丘思兰,谭志灿,黄洁靖,等.Box-Behnken效应面法优化侧柏叶总黄酮提取工艺[J].云南中医学院学报,2015,38(1):31-37.
[2]王祝年,赖富丽,王茂媛,等.牛大力根的化学成分研究[J].热带作物学报,2011,32(12):2378-2380.
[3]王春华,王英,王国才,等.牛大力的化学成分研究[J].中草药,2008,39(7):972-975.
[4]王呈文,陈光英,宋小平,等.牛大力的化学成分研究[J].中草药,2014,45(11):1515-1520.
[5]郑元升,蒲含林,麻建军.牛大力多糖对小鼠T淋巴细胞增殖的双向调节作用[J].广东药学院学报,2008,24(1):58-61.
[6]黄翔,王晓平,陈晓白.中药牛大力抗疲劳抗应激作用的实验研究[J].玉林师范学院学报,2014,35(2):55-58.
[7]王呈文,纪明慧,舒火明,等.牛大力总黄酮提取工艺及不同萃取物的抗氧化活性研究[J].化学研究与应用,2013,25(5):713-717.
[8]周添浓,刘丹丹,唐立海,等.牛大力对四氯化碳及酒精所致小鼠急性肝损伤的保护作用[J].时珍国医国药,2009,20(10):2585-2587.
[9]丁新爱.独一味提取工艺研究[J].河南中医学院学报,2006,21(2):40.
[10]张峻菁,冯珊娜.正交设计法优选蒲桃中总黄酮的提取工艺[J].今日药学,2015,25(8):571-573.
[11]丘思兰,谭志灿,黄洁靖,等.Box-Behnken效应面法优化侧柏叶总黄酮提取工艺[J].云南中医学院学报,2015,38(1):31-37.