丛枝菌根真菌对杜鹃花耐热性的影响
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摘要
为研究丛枝菌根真菌对杜鹃花耐热性的影响,以杜鹃花品种‘笔止’ Rhododendron ‘Bi Zhi’为试验材料,利用人工气候法,研究根内球囊霉Glimus intraradices(Gi),摩西球囊霉Glomus mosseae(Gm)和幼套球囊霉Glomus etunicatum(Ge)等3种丛枝菌根真菌(arbuscular mycorrhizal fungi, AMF)在高温胁迫下对杜鹃花幼苗生理生化指标的影响,比较高温胁迫下接种前后杜鹃花叶片解剖结构中栅栏组织厚度、海绵组织厚度、栅栏海绵组织比的差异,筛选出最佳AMF。结果表明:高温胁迫下,接种AMF不仅延缓了叶片的可溶性糖、可溶性蛋白质、游离脯氨酸和叶绿素质量分数的下降,并且使细胞膜透性和丙二醛摩尔质量分数保持相对较低的水平。此外,接种AMF后杜鹃花叶片解剖结构受损较轻,栅栏海绵组织比明显提高。根据平均隶属函数度对AMF提高杜鹃花的耐热性进行评价,其从强到弱排序为Gi, Gm和Ge,其中接种Gi的效果最为显著。因此, AMF能在一定程度上提高杜鹃花品种‘笔止’的耐热性。
To determine the effects of arbuscular mycorrhizal fungi(AMF) on heat resistance of Rhododendron and to choose the best AMF, Rhododendron ‘Bi Zhi' was selected as the test material. Three AMF, Glomus intraradices(Gi), Glomus mosseae(Gm), and Glomus etunicatum(Ge) were used to study the effects on physiological and biochemical indexes of Rhododendron under high temperature stress. Analysis included the average subordinate function method to comprehensively evaluate the heat resistance with the three kinds of AMF and azalea. A comparison of the leaf anatomic structural characteristics before and after inoculation with the three fungi was conducted for heat stress by observing the performance. Results showed that under high temperature stress, the three AMF could colonize azalea roots. The colonization percentage of Gi was the highest(49.6%), while Ge the lest(37.4%). The contents of soluble sugar, soluble protein, chlorophyll, and proline increased compared to the control. At the same time the contents of malondialdehyde(MDA) and membrane permeability in azalea inoculated Gi stayed at a relatively low level. Furthermore, the anatomic structure of leaves inoculated with AMF was less damaged, and the ratio of palisade sponge tissue of the AMF inoculated leaf was much higher than that of the non-AMF control. According to the average subordinate function method,the heat resistance for azalea with AMF was in the order Gi > Gm > Ge. Thus, AMF could improve the heat resistance of the azalea cultivars to some extent.
To determine the effects of arbuscular mycorrhizal fungi(AMF) on heat resistance of Rhododendron and to choose the best AMF, Rhododendron ‘Bi Zhi' was selected as the test material. Three AMF, Glomus intraradices(Gi), Glomus mosseae(Gm), and Glomus etunicatum(Ge) were used to study the effects on physiological and biochemical indexes of Rhododendron under high temperature stress. Analysis included the average subordinate function method to comprehensively evaluate the heat resistance with the three kinds of AMF and azalea. A comparison of the leaf anatomic structural characteristics before and after inoculation with the three fungi was conducted for heat stress by observing the performance. Results showed that under high temperature stress, the three AMF could colonize azalea roots. The colonization percentage of Gi was the highest(49.6%), while Ge the lest(37.4%). The contents of soluble sugar, soluble protein, chlorophyll, and proline increased compared to the control. At the same time the contents of malondialdehyde(MDA) and membrane permeability in azalea inoculated Gi stayed at a relatively low level. Furthermore, the anatomic structure of leaves inoculated with AMF was less damaged, and the ratio of palisade sponge tissue of the AMF inoculated leaf was much higher than that of the non-AMF control. According to the average subordinate function method,the heat resistance for azalea with AMF was in the order Gi > Gm > Ge. Thus, AMF could improve the heat resistance of the azalea cultivars to some extent.
引文
[1]林斌.中国杜鹃花(园艺品种及应用)[M].北京:中国林业出版社, 2008.
[2]申惠翡,赵冰.杜鹃花品种耐热性评价及其生理机制研究[J].植物生理学报, 2018, 54(2):335-345.SHEN Huifei, ZHAO Bing. Study on evaluation of heat tolerance and its physiological mechanisms in Rhododendron cultivars[J]. Plant Physiol J, 2018, 54(2):335-345.
[3]梁雯,赵冰,黄文梅.热锻炼对杜鹃花耐热性的影响[J].浙江农林大学学报, 2018, 35(2):284-290.LIANG Wen, ZHAO Bing, HUANG Wenmei. Heat-resistance of Rhododendron with a heat acclimation pretreatment[J]. J Zhejiang A&F Univ, 2018, 35(2):284-290.
[4]高晓宁,赵冰,刘旭梅,等. 4个杜鹃花品种对干旱胁迫的生理响应及抗旱性评价[J].浙江农林大学学报,2017, 34(4):597-607.GAO Xiaoning, ZHAO Bing, LIU Xumei, et al. Physiological response to drought stress and drought resistance evaluation of four Rhododendron cultivars[J]. J Zhejiang A&F Univ, 2017, 34(4):597-607.
[5]高晓宁,梁雯,赵冰.外源水杨酸对2个杜鹃花品种抗旱性的影响[J].西北林学院学报, 2018, 33(3):131-136.GAO Xiaoning, LIANG Wen, ZHAO Bing. Effects of exogenous salicylic acid on heat-resistance of Rhododendron hybridum[J]. J Northwest For Univ, 2018, 33(3):131-136.
[6]刘润进,焦惠,李岩,等.丛枝菌根真菌物种多样性研究进展[J].应用生态学报, 2009, 20(9):2301-2307.LIU Runjin, JIAO Hui, LI Yan, et al. Advances in the study of arbuscular mycorrhizal fungi onspecies diversity[J].China J Appl Ecol, 2009, 20(9):2301-2307.
[7]马通,刘润进,李敏.丛枝菌根真菌对生菜耐热性的效应[J].植物生理学报, 2015, 51(11):1919-1926.MA Tong, LIU Runjin, LI Min. Effects of arbuscular mycorrhizal fungi on heat-tolerance of Lactuca satica L.[J].Plant Physiol J, 2015, 51(11):1919-1926.
[8]刘慧,陈薇,周勇,等.内生真菌和丛枝菌根真菌对羊草生长的影响[J].植物生态学报, 2015, 39(5):477-485.LIU Hui, CHEN Wei, ZHOU Yong, et al. Effects of endophyte and arbuscular mycorrhizal fungi on growth of Leymus chinensis[J]. Chin J Plant Ecol, 2015, 39(5):477-485.
[9]马放,苏蒙,王立,等.丛枝菌根真菌对小麦生长的影响[J].生态学报, 2014, 34(21):6107-6114.MA Fang, SU Meng, WANG Li, et al. Effects of arbuscular mycorrhizal fungi(AMF)on the growth of wheat[J]. Acta Ecol Sin, 2014, 34(21):6107-6114.
[10]陈可,孙吉庆,刘润进,等.丛枝菌根真菌对西瓜嫁接苗生长和根系防御性酶活性的影响[J].应用生态学报, 2013, 24(1):135-141.CHEN Ke, SUN Jiqing, LIU Runjin, et al. Effects of arbuscular mycorrhizal fungus on the seedling growth of grafted watermelon and the defensive enzyme activities in the seedling root[J]. Chin J Appl Ecol, 2013, 24(1):135-141.
[11]刘爱荣,陈双臣,刘艳英,等.丛枝菌根真菌对低温下黄瓜幼苗光合生理和抗氧化酶活性的影响[J].生态学报, 2011, 31(12):3497-3503.LIU Airong, CHEN Shuangchen, LIU Yanying, et al. Effects of AM fungi on leaf photosynthetic physiological parameters and antioxidant enzyme activities under low temperature[J]. Acta Ecol Sin, 2011, 31(12):3497-3503.
[12]张珊珊,康洪梅,杨文忠,等.干旱胁迫下AMF对云南蓝果树幼苗生长和光合特征的影响[J].生态学报,2016, 36(21):6850-6862.ZHANG Shanshan, KANG Hongmei, YANG Wenzhong, et al. Effects of arbuscular mycorrhizal fungi on growth and photosynthetic characteristics of Nyssa yunnanensis seedlings under drought stress[J]. Acta Ecol Sin, 2016, 36(21):6850-6862.
[13]郭绍霞,王政军,赵廷武.高温胁迫下AM真菌对牡丹叶片解剖结构和蒸腾特性的影响[J].青岛农业大学学报(自然科学版), 2011, 28(3):165-167.GUO Shaoxia, WANG Zhengjun, ZHAO Tingwu. Effects of Arbuscular mycorrhizal fungi on leaf anatomical structure and transpiration of Paeonia suffruticosa under high temperature stress[J]. J Qingdao Agric For Univ Nat Sci Ed,2011, 28(3):165-167.
[14]孙吉庆,刘润进,李敏.丛枝菌根真菌提高植物抗逆性的效应及其机制研究进展[J].植物生理学报,2012, 48(9):845-852.SUN Jiqing, LIU Runjin, LI Min. Advances in the study of increasing plant stress resistance and mechanisms by arbuscular mycorrhizal fungi[J]. Plant Physiol J, 2012, 48(9):845-852.
[15]路文静,李奕松.植物生理学实验教程[M].北京:中国林业出版社, 2012.
[16]魏望,施富超,王东玮,等.多倍体植物抗逆性研究进展[J].西北植物学报, 2016, 36(4):846-856.WEI Wang, SHI Fuchao, WANG Dongwei, et al. Advances in polyploid plant resistance[J]. Acta Bot Boreal-Occident Sin, 2016, 36(4):846-856.
[17]李素美,王银桥,刘润进.特殊生境中丛枝菌根真菌多样性[J].应用生态学报, 2013, 24(11):3325-3332.LI Sumei, WANG Yinqiao, LIU Runjin. Diversity of arbuscular mycorrhizal fungi in special habitats[J]. Chin J Appl Ecol, 2013, 24(11):3325-3332.
[18]田密,陈应龙,李敏,等.丛枝菌根结构与功能研究进展[J].应用生态学报, 2013, 24(8):2369-2376.TIAN Mi, CHEN Yinglong, LI Min, et al. Structure and function of arbuscular mycorrhiza[J]. Chin J Appl Ecol,2013, 24(8):2369-2376.
[19]赵平娟,安锋,唐明.丛枝菌根真菌对连翘幼苗抗旱性的影响[J].西北植物学报, 2007, 27(2):396-399.ZHAO Pingjuan, AN Feng, TANG Ming. Effect of arbuscular mycorrhizal fungi on drought resistance of Forsythia suspensa[J]. Acta Bot Boreal-Occident Sin, 2007, 27(2):396-399.
[20]赵匠,徐佳晶,李霞.丛枝菌根真菌对黄檗耐盐能力的影响[J].东北林业大学学报, 2016, 44(11):74-77.ZHAO Jiang, XU Jiajing, LI Xia. Effects of Arbuscular mycorrhizal fungi on salt tolerance ability of Phellodendron amurense[J]. J Northeast For Univ, 2016, 44(11):74-77.
[21]曹岩坡,代鹏,戴素英.丛枝菌根真菌(AMF)对盐胁迫下芦笋植株渗透调节物质及抗氧化酶活性的影响[J].西南大学学报(自然科学版), 2017, 39(5):43-48.CAO Yanpo, DAI Peng, DAI Suying. Effects of arbuscular mycorrhiza fungi(AMF)on osmoregulation substances and antioxidant enzyme activities of Asparagus plant under salt stress[J]. J Southwest Univ Nat Sci Ed, 2017, 39(5):43-48.
[22]许平辉,王飞权,齐玉岗,等.丛枝菌根真菌对茶树抗旱性的影响[J].西北农业学报, 2017, 26(7):1033-1040.XU Pinghui, WANG Feiquan, QI Yugang, et al. Effect of arbuscular mycorrhiza fungi on drought resistance in tea plant[J]. J Northwest Agric, 2017, 26(7):1033-1040.
[23]韩冰,徐刚,郭世荣,等.丛枝菌根真菌对苗期黄瓜生长及生理特性的影响[J].江苏农业学报, 2012, 28(6):1392-1397.HAN Bing, XU Gang, GUO Shirong, et al. Effects of arbuscular mycorrhiz fungi on growth and physiological characteristics of cucumber seedlings[J]. J Jiangsu Agric Sci, 2012, 28(6):1392-1397.
[24]闫妍,孙超,于贤昌,等.低温胁迫对接种丛枝菌根真菌番茄幼苗生理特性的影响[J].中国农业大学学报,2011, 16(6):64-69.YAN Yan, SUN Chao, YU Xianchang, et al. Effects of arbuscular mycorrhizal fungi on physiological properties of tomato seedlings under low temperature stress[J]. J China Agric Univ, 2011, 16(6):64-69.
[2]申惠翡,赵冰.杜鹃花品种耐热性评价及其生理机制研究[J].植物生理学报, 2018, 54(2):335-345.SHEN Huifei, ZHAO Bing. Study on evaluation of heat tolerance and its physiological mechanisms in Rhododendron cultivars[J]. Plant Physiol J, 2018, 54(2):335-345.
[3]梁雯,赵冰,黄文梅.热锻炼对杜鹃花耐热性的影响[J].浙江农林大学学报, 2018, 35(2):284-290.LIANG Wen, ZHAO Bing, HUANG Wenmei. Heat-resistance of Rhododendron with a heat acclimation pretreatment[J]. J Zhejiang A&F Univ, 2018, 35(2):284-290.
[4]高晓宁,赵冰,刘旭梅,等. 4个杜鹃花品种对干旱胁迫的生理响应及抗旱性评价[J].浙江农林大学学报,2017, 34(4):597-607.GAO Xiaoning, ZHAO Bing, LIU Xumei, et al. Physiological response to drought stress and drought resistance evaluation of four Rhododendron cultivars[J]. J Zhejiang A&F Univ, 2017, 34(4):597-607.
[5]高晓宁,梁雯,赵冰.外源水杨酸对2个杜鹃花品种抗旱性的影响[J].西北林学院学报, 2018, 33(3):131-136.GAO Xiaoning, LIANG Wen, ZHAO Bing. Effects of exogenous salicylic acid on heat-resistance of Rhododendron hybridum[J]. J Northwest For Univ, 2018, 33(3):131-136.
[6]刘润进,焦惠,李岩,等.丛枝菌根真菌物种多样性研究进展[J].应用生态学报, 2009, 20(9):2301-2307.LIU Runjin, JIAO Hui, LI Yan, et al. Advances in the study of arbuscular mycorrhizal fungi onspecies diversity[J].China J Appl Ecol, 2009, 20(9):2301-2307.
[7]马通,刘润进,李敏.丛枝菌根真菌对生菜耐热性的效应[J].植物生理学报, 2015, 51(11):1919-1926.MA Tong, LIU Runjin, LI Min. Effects of arbuscular mycorrhizal fungi on heat-tolerance of Lactuca satica L.[J].Plant Physiol J, 2015, 51(11):1919-1926.
[8]刘慧,陈薇,周勇,等.内生真菌和丛枝菌根真菌对羊草生长的影响[J].植物生态学报, 2015, 39(5):477-485.LIU Hui, CHEN Wei, ZHOU Yong, et al. Effects of endophyte and arbuscular mycorrhizal fungi on growth of Leymus chinensis[J]. Chin J Plant Ecol, 2015, 39(5):477-485.
[9]马放,苏蒙,王立,等.丛枝菌根真菌对小麦生长的影响[J].生态学报, 2014, 34(21):6107-6114.MA Fang, SU Meng, WANG Li, et al. Effects of arbuscular mycorrhizal fungi(AMF)on the growth of wheat[J]. Acta Ecol Sin, 2014, 34(21):6107-6114.
[10]陈可,孙吉庆,刘润进,等.丛枝菌根真菌对西瓜嫁接苗生长和根系防御性酶活性的影响[J].应用生态学报, 2013, 24(1):135-141.CHEN Ke, SUN Jiqing, LIU Runjin, et al. Effects of arbuscular mycorrhizal fungus on the seedling growth of grafted watermelon and the defensive enzyme activities in the seedling root[J]. Chin J Appl Ecol, 2013, 24(1):135-141.
[11]刘爱荣,陈双臣,刘艳英,等.丛枝菌根真菌对低温下黄瓜幼苗光合生理和抗氧化酶活性的影响[J].生态学报, 2011, 31(12):3497-3503.LIU Airong, CHEN Shuangchen, LIU Yanying, et al. Effects of AM fungi on leaf photosynthetic physiological parameters and antioxidant enzyme activities under low temperature[J]. Acta Ecol Sin, 2011, 31(12):3497-3503.
[12]张珊珊,康洪梅,杨文忠,等.干旱胁迫下AMF对云南蓝果树幼苗生长和光合特征的影响[J].生态学报,2016, 36(21):6850-6862.ZHANG Shanshan, KANG Hongmei, YANG Wenzhong, et al. Effects of arbuscular mycorrhizal fungi on growth and photosynthetic characteristics of Nyssa yunnanensis seedlings under drought stress[J]. Acta Ecol Sin, 2016, 36(21):6850-6862.
[13]郭绍霞,王政军,赵廷武.高温胁迫下AM真菌对牡丹叶片解剖结构和蒸腾特性的影响[J].青岛农业大学学报(自然科学版), 2011, 28(3):165-167.GUO Shaoxia, WANG Zhengjun, ZHAO Tingwu. Effects of Arbuscular mycorrhizal fungi on leaf anatomical structure and transpiration of Paeonia suffruticosa under high temperature stress[J]. J Qingdao Agric For Univ Nat Sci Ed,2011, 28(3):165-167.
[14]孙吉庆,刘润进,李敏.丛枝菌根真菌提高植物抗逆性的效应及其机制研究进展[J].植物生理学报,2012, 48(9):845-852.SUN Jiqing, LIU Runjin, LI Min. Advances in the study of increasing plant stress resistance and mechanisms by arbuscular mycorrhizal fungi[J]. Plant Physiol J, 2012, 48(9):845-852.
[15]路文静,李奕松.植物生理学实验教程[M].北京:中国林业出版社, 2012.
[16]魏望,施富超,王东玮,等.多倍体植物抗逆性研究进展[J].西北植物学报, 2016, 36(4):846-856.WEI Wang, SHI Fuchao, WANG Dongwei, et al. Advances in polyploid plant resistance[J]. Acta Bot Boreal-Occident Sin, 2016, 36(4):846-856.
[17]李素美,王银桥,刘润进.特殊生境中丛枝菌根真菌多样性[J].应用生态学报, 2013, 24(11):3325-3332.LI Sumei, WANG Yinqiao, LIU Runjin. Diversity of arbuscular mycorrhizal fungi in special habitats[J]. Chin J Appl Ecol, 2013, 24(11):3325-3332.
[18]田密,陈应龙,李敏,等.丛枝菌根结构与功能研究进展[J].应用生态学报, 2013, 24(8):2369-2376.TIAN Mi, CHEN Yinglong, LI Min, et al. Structure and function of arbuscular mycorrhiza[J]. Chin J Appl Ecol,2013, 24(8):2369-2376.
[19]赵平娟,安锋,唐明.丛枝菌根真菌对连翘幼苗抗旱性的影响[J].西北植物学报, 2007, 27(2):396-399.ZHAO Pingjuan, AN Feng, TANG Ming. Effect of arbuscular mycorrhizal fungi on drought resistance of Forsythia suspensa[J]. Acta Bot Boreal-Occident Sin, 2007, 27(2):396-399.
[20]赵匠,徐佳晶,李霞.丛枝菌根真菌对黄檗耐盐能力的影响[J].东北林业大学学报, 2016, 44(11):74-77.ZHAO Jiang, XU Jiajing, LI Xia. Effects of Arbuscular mycorrhizal fungi on salt tolerance ability of Phellodendron amurense[J]. J Northeast For Univ, 2016, 44(11):74-77.
[21]曹岩坡,代鹏,戴素英.丛枝菌根真菌(AMF)对盐胁迫下芦笋植株渗透调节物质及抗氧化酶活性的影响[J].西南大学学报(自然科学版), 2017, 39(5):43-48.CAO Yanpo, DAI Peng, DAI Suying. Effects of arbuscular mycorrhiza fungi(AMF)on osmoregulation substances and antioxidant enzyme activities of Asparagus plant under salt stress[J]. J Southwest Univ Nat Sci Ed, 2017, 39(5):43-48.
[22]许平辉,王飞权,齐玉岗,等.丛枝菌根真菌对茶树抗旱性的影响[J].西北农业学报, 2017, 26(7):1033-1040.XU Pinghui, WANG Feiquan, QI Yugang, et al. Effect of arbuscular mycorrhiza fungi on drought resistance in tea plant[J]. J Northwest Agric, 2017, 26(7):1033-1040.
[23]韩冰,徐刚,郭世荣,等.丛枝菌根真菌对苗期黄瓜生长及生理特性的影响[J].江苏农业学报, 2012, 28(6):1392-1397.HAN Bing, XU Gang, GUO Shirong, et al. Effects of arbuscular mycorrhiz fungi on growth and physiological characteristics of cucumber seedlings[J]. J Jiangsu Agric Sci, 2012, 28(6):1392-1397.
[24]闫妍,孙超,于贤昌,等.低温胁迫对接种丛枝菌根真菌番茄幼苗生理特性的影响[J].中国农业大学学报,2011, 16(6):64-69.YAN Yan, SUN Chao, YU Xianchang, et al. Effects of arbuscular mycorrhizal fungi on physiological properties of tomato seedlings under low temperature stress[J]. J China Agric Univ, 2011, 16(6):64-69.