咳喘六味合剂质量标准提高研究
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摘要
目的完善龙华医院院内制剂咳喘六味合剂的质量控制标准。方法应用TLC法对咳喘六味合剂中的黄芩、细辛进行定性鉴别;应用HPLC法对麻黄、黄芩进行含量测定。采用Welch-C18柱(4.6mm×250mm,5μm);以乙腈-0.1%磷酸溶液(4∶96)及甲醇-0.1%磷酸溶液(47∶53)为流动相;检测波长分别为206、278nm;流速为1.0ml/min。结果 TLC法显示与黄芩、细辛药材特征荧光斑点相符合的斑点,且阴性对照溶液无干扰。盐酸麻黄碱在12.04～301.00μg/ml范围内线性关系良好(r=1.000 0),平均回收率为101.7%,RSD为1.5%;盐酸伪麻黄碱在7.98～199.40μg/ml范围内线性关系良好(r=1.000 0),平均回收率为101.6%,RSD为2.4%。黄芩苷在5.18～129.50μg/ml范围内与峰面积呈良好线性关系(r=0.999 9),平均回收率为101.0%,RSD为0.3%。结论本实验建立的定性鉴别和含量测定方法简单可行,可以作为咳喘六味合剂的质量控制标准。
Objective To improve the quality control standard of the hospital preparation Kechuan Liuwei oral liquid in Longhua Hospital.Methods TLC was used for qualitative identification of Scutellaria baicalensis Georgi and Asarum sieboldi Mig in the Kechuan Liuwei oral liquid.Ephedrine and Baicalin content in ephedra and Scutellaria were determined by HPLC with Welch-C18column(4.6mm ×250mm,5μm).Acetonitrile-0.1% phosphoric acid solution(4∶96)and methanol-0.1%phosphoric acid solution(47∶53)were used as mobile phase.The detection wavelengths were 206 nm and 278 nm respectively.The flow rate was 1.0ml/min.Results The TLC spots of Scutellaria baicalensis Georgi and asarum were clear without interference of the negative control.The linear range of Ephedrine hydrochloride was within 12.04-301.00μg/ml(r=0.999 9).The average recovery was 101.7%(RSD=1.5%).The linear range of Pseudoephedrine hydrochloride was within 7.98-199.40μg/ml(r=0.999 9).The average recovery was 101.6%(RSD=2.4%).The linear range of Baicalin was within 5.18-129.50μg/ml(r=0.999 9).The average recovery was 101.0%(RSD=0.3%).Conclusion The qualitative identification and the active ingredient assay method established in this experiment were simple and feasible.Those methods can be used as the quality control standard for Kechuan Liuwei oral liquid.
Objective To improve the quality control standard of the hospital preparation Kechuan Liuwei oral liquid in Longhua Hospital.Methods TLC was used for qualitative identification of Scutellaria baicalensis Georgi and Asarum sieboldi Mig in the Kechuan Liuwei oral liquid.Ephedrine and Baicalin content in ephedra and Scutellaria were determined by HPLC with Welch-C18column(4.6mm ×250mm,5μm).Acetonitrile-0.1% phosphoric acid solution(4∶96)and methanol-0.1%phosphoric acid solution(47∶53)were used as mobile phase.The detection wavelengths were 206 nm and 278 nm respectively.The flow rate was 1.0ml/min.Results The TLC spots of Scutellaria baicalensis Georgi and asarum were clear without interference of the negative control.The linear range of Ephedrine hydrochloride was within 12.04-301.00μg/ml(r=0.999 9).The average recovery was 101.7%(RSD=1.5%).The linear range of Pseudoephedrine hydrochloride was within 7.98-199.40μg/ml(r=0.999 9).The average recovery was 101.6%(RSD=2.4%).The linear range of Baicalin was within 5.18-129.50μg/ml(r=0.999 9).The average recovery was 101.0%(RSD=0.3%).Conclusion The qualitative identification and the active ingredient assay method established in this experiment were simple and feasible.Those methods can be used as the quality control standard for Kechuan Liuwei oral liquid.
引文
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[2]倪伟,吴银根,张惠勇,等.咳喘落治疗支气管哮喘寒哮证多中心临床研究[J].上海中医药大学学报,2007,21(3):43-45.
[3]国家药典委员会.中华人民共和国药典(四部):2015年版[S].北京:中国医药科技出版社,2015:57.
[4]顾俊.川芎茶调散的质量标准研究[J].中国冶金工业医学杂志,2014,31(4):474-475.
[5]周斌,刘可越,刘海军,等.RP-HPLC法测定麻黄配方颗粒中麻黄碱和伪麻黄碱的含量[J].天津药学,2009,21(4):9-11.
[6]先春,龚小见,赵超,等.虎耳草的化学成分研究[J].中国实验方剂学杂志,2012,18(10):124-126.