微波消融对舌癌Cal-27裸鼠移植瘤凋亡机制的初步研究
|
摘要
目的:微波消融作用于舌癌Cal-27裸鼠移植瘤模型,探讨其可能的抗肿瘤作用机制。方法:建立20只舌癌Cal-27裸鼠皮下移植瘤模型,随机分为空白对照组、50℃1 min组、50℃2 min组、55℃2 min组(n=5),微波消融作用于裸鼠移植瘤。采用HE染色光镜下观察肿瘤细胞凋亡情况,免疫组化SP法检测其中Caspase-3和PCNA蛋白表达。结果:HE染色结果表明微波消融可诱导Cal-27细胞发生明显凋亡。免疫组化结果显示,与对照组相比,微波消融各组Caspase-3蛋白的表达有明显上调趋势(P <0. 05),PCNA蛋白的表达有明显下调趋势(P <0. 05)。微波消融55℃2 min组Caspase-3蛋白的表达显著升高更明显(P <0. 01),PCNA蛋白的表达显著降低更明显(P <0. 01)。结论:微波消融对舌癌增殖起抑制作用,可引起其细胞凋亡,其机制可能与上调Caspase-3信号通路、下调PCNA信号通路有关。
Objective: To study the apoptosis mechanism of microwave ablation( MA) in tongue cancer Cal-27 cell transplanted tumor in nude mice. Methods: 20 nude mice with Cal-27 cell subcutaneously transplanted tumor were randomly divided into blank control group,50 ℃ 1 min MA group,50 ℃ 2 min MA group and 55 ℃ 2 min MA group( n = 5) respecitvely,and were treated with MA with indiated perameters respectively. The apoptosis of tumor cells was observed by HE staining. The expression of Caspase-3 and PCNA protein was detected by immunohistochemical SP method. Results: HE staining showed that microwave ablation induced apoptosis of the tumor cells. Immunohistochemical results showed that the expression of Caspase-3 protein was significantly up-regulated in the microwave ablation groups compared with the control group( P < 0. 05),and the expression of PCNA protein was significantly decreased( P < 0. 05). In microwave ablation 55 ℃ 2 min group,the expression of Caspase-3 protein was increased more( P <0. 01),and the expression of PCNA protein was decreased more( P < 0. 01). Conclusion: Microwave ablation may inhibite the proliferation of tongue cancer cells by inducing apoptosis of the cells. The mechanism may be related to the up-regulation of Caspase-3 signaling pathway and down-regulation of PCNA signaling pathway.
Objective: To study the apoptosis mechanism of microwave ablation( MA) in tongue cancer Cal-27 cell transplanted tumor in nude mice. Methods: 20 nude mice with Cal-27 cell subcutaneously transplanted tumor were randomly divided into blank control group,50 ℃ 1 min MA group,50 ℃ 2 min MA group and 55 ℃ 2 min MA group( n = 5) respecitvely,and were treated with MA with indiated perameters respectively. The apoptosis of tumor cells was observed by HE staining. The expression of Caspase-3 and PCNA protein was detected by immunohistochemical SP method. Results: HE staining showed that microwave ablation induced apoptosis of the tumor cells. Immunohistochemical results showed that the expression of Caspase-3 protein was significantly up-regulated in the microwave ablation groups compared with the control group( P < 0. 05),and the expression of PCNA protein was significantly decreased( P < 0. 05). In microwave ablation 55 ℃ 2 min group,the expression of Caspase-3 protein was increased more( P <0. 01),and the expression of PCNA protein was decreased more( P < 0. 01). Conclusion: Microwave ablation may inhibite the proliferation of tongue cancer cells by inducing apoptosis of the cells. The mechanism may be related to the up-regulation of Caspase-3 signaling pathway and down-regulation of PCNA signaling pathway.
引文
[1]Bose P,Brockton NT,Dort JC.Head and neck cancer:From anatomy to biology[J].Int J Cancer,2013,133(9):2013-2023.
[2]Parkin DM,Bray F,Ferlay J,et al.Global cancer statistics,2002[J].CA Cancer J Clin,2005,55(2):74-108.
[3]Brace CL.Radiofrequency and microwave ablation of the liver,lung,kidney,and bone:What are the differences?[J].Curr Probl Diagn Radiol,2009,38(3):135-143.
[4]Gravante G,Ong SL,Metcalfe MS,et al.Hepatic microwave ablation:A review of the histological changes following thermal damage[J].Liver Int,2008,28(7):911-921.
[5]Lubner MG,Brace CL,Hinshaw JL,et al.Microwave tumor ablation:Mechanism of action,clinical results,and devices[J].J Vasc Interv Radiol,2010,21(8 Suppl):S192-203.
[6]李欣.超声引导下微波消融治疗肝癌的研究进展[J].肿瘤学杂志,2010,16(7):523-526.
[7]Poggi G,Tosoratti N,Montagna B,et al.Microwave ablation of hepatocellular carcinoma[J].World J Hepatol,2015,7(25):2578-2589.
[8]Fukami T,Nakasu S,Baba K,et al.Hyperthermia induces translocation of apoptosis-inducing factor(AIF)and apoptosis in human glioma cell lines[J].J Neurooncol,2004,70(3):319-331.
[9]Motomura T,Ueda K,Ohtani S,et al.Evaluation of systemic external microwave hyperthermia for treatment of pleural metastasis in orthotopic lung cancer model[J].Oncol Rep,2010,24(3):591-598.
[10]Huang Q,Li F,Liu X,et al.Caspase 3-mediated stimulation of tumor cell repopulation during cancer radiotherapy[J].Nat Med,2011,17(7):860-866.
[11]Xing G,Kirouac K,Shin YJ,et al.Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA[J].Mol Microbiol,2009,71(3):678-691.
[12]李向新,蒋蕾,白玲,等.Livin和PCNA在口腔鳞癌中的表达及意义[J].实用口腔医学杂志,2011,27(4):562-565.
[13]赵阳,李晓军,隋昕,等.PCNA和Caspase-3在肺癌组织中的表达及意义[J].细胞与分子免疫学杂志,2010,26(2):154-156.
[2]Parkin DM,Bray F,Ferlay J,et al.Global cancer statistics,2002[J].CA Cancer J Clin,2005,55(2):74-108.
[3]Brace CL.Radiofrequency and microwave ablation of the liver,lung,kidney,and bone:What are the differences?[J].Curr Probl Diagn Radiol,2009,38(3):135-143.
[4]Gravante G,Ong SL,Metcalfe MS,et al.Hepatic microwave ablation:A review of the histological changes following thermal damage[J].Liver Int,2008,28(7):911-921.
[5]Lubner MG,Brace CL,Hinshaw JL,et al.Microwave tumor ablation:Mechanism of action,clinical results,and devices[J].J Vasc Interv Radiol,2010,21(8 Suppl):S192-203.
[6]李欣.超声引导下微波消融治疗肝癌的研究进展[J].肿瘤学杂志,2010,16(7):523-526.
[7]Poggi G,Tosoratti N,Montagna B,et al.Microwave ablation of hepatocellular carcinoma[J].World J Hepatol,2015,7(25):2578-2589.
[8]Fukami T,Nakasu S,Baba K,et al.Hyperthermia induces translocation of apoptosis-inducing factor(AIF)and apoptosis in human glioma cell lines[J].J Neurooncol,2004,70(3):319-331.
[9]Motomura T,Ueda K,Ohtani S,et al.Evaluation of systemic external microwave hyperthermia for treatment of pleural metastasis in orthotopic lung cancer model[J].Oncol Rep,2010,24(3):591-598.
[10]Huang Q,Li F,Liu X,et al.Caspase 3-mediated stimulation of tumor cell repopulation during cancer radiotherapy[J].Nat Med,2011,17(7):860-866.
[11]Xing G,Kirouac K,Shin YJ,et al.Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA[J].Mol Microbiol,2009,71(3):678-691.
[12]李向新,蒋蕾,白玲,等.Livin和PCNA在口腔鳞癌中的表达及意义[J].实用口腔医学杂志,2011,27(4):562-565.
[13]赵阳,李晓军,隋昕,等.PCNA和Caspase-3在肺癌组织中的表达及意义[J].细胞与分子免疫学杂志,2010,26(2):154-156.