油菜品质性状分子标记开发及QTL定位研究进展
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摘要
随着油菜品质性状遗传规律逐渐被挖掘及品质性状相关的分子标记和QTL定位的研究不断深入,相关的研究成果越来越多,这些研究成果对进一步利用分子标记辅助选择(MAS)加快育种进程具有重要意义。通过对油菜中几个重要的脂肪酸相关的分子标记和QTL定位进行综述,对已有的研究报道中的研究结果进行探讨和总结,提出存在的问题并进行展望。
With the development of genetic analysis,molecular marker exploration and QTL mapping of quality traits in rapeseed,more and more researches have been made with results which could be used to accelerate the breeding process with molecular marker assisted selection(MAS). This article summarized the research progress in molecular markers analysis and QTL mapping of a few important fatty acid contents. Finally,the accomplished research results were discussed and summarized; meanwhile,the existing problems and future prospects were also pointed out.
With the development of genetic analysis,molecular marker exploration and QTL mapping of quality traits in rapeseed,more and more researches have been made with results which could be used to accelerate the breeding process with molecular marker assisted selection(MAS). This article summarized the research progress in molecular markers analysis and QTL mapping of a few important fatty acid contents. Finally,the accomplished research results were discussed and summarized; meanwhile,the existing problems and future prospects were also pointed out.
引文
[1]王汉中.中国油菜品种改良的中长期发展战略[J].中国油料作物学报,2004,26(3):98-101.
[2]ZHAO J Y,BECKER H C,ZHANG D Q,et al. Conditional QTL mapping of oil content in rapeseed with respect to protein content and traits related to plant development and grain yield[J]. Theoretical and Applied Genetics,2006,113(1):33-38.
[3]张宁.用分子标记技术初步解析甘蓝型油菜种子含油量QTL簇qc O. C2[D].武汉:华中农业大学,2009.
[4]郭世星.甘蓝型油菜含油量等重要品质性状的遗传分析与QTL定位[D].成都:四川农业大学,2010.
[5]WANG X,WANG H,LONG Y,et al. Identification of QTLs associated with oil content in a high-oil Brassica napus cultivar and construction of a high-density consensus map for QTLs comparison in B. napus[J]. PLOS ONE,2013,8(12):e80569.
[6]TEH L,MOLLERS C. Genetic variation and inheritance of phytosterol and oil content in a doubled haploid population derived from the winter oilseed rape Sansibar×Oase cross[J]. Theoretical and Applied Genetics,2016,129(1):181-199.
[7]SUN M Y,HUA W,LIU J,et al. Design of new genome-and gene-sourced primers and identification of QTL for seed oil content in a specially high-oil Brassica napus cultivar[J]. PLOS ONE,2012,7(10):e47037.
[8]RABOANATAHIRY N,CHAO H B,GUO L X,et al. Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus[J]. BMC Genomics,2017,18:776-790.
[9]孔祥瑞.必需微量元素的营养生理及临床意义[M].合肥:安徽科学出版社,1982.
[10]SHEN J H,MA Q,SHEN S R,et al. Effect ofα-linolenic acid on streptozotocin-induced diabetic retinopathy indices in vivo[J]. Archives of Medical Research,2013,44(7):514-520.
[11]潘勇.α-亚麻酸在体内外的吸收和转化为长链多不饱和脂肪酸效率的研究[D].无锡:江南大学,2016.
[12]HU X,SULLIVAN-GILBERT M,GUPTA M,et al. Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canol(Brassica napus L.)[J]. Theoretical and Applied Genetics,2006,113(3):497-507.
[13]JAVIDFAR F,RIPLEY V L,ROSLINSKY V. Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape(Brassica napus)[J]. Plant Breeding,2006,125(1):65-71.
[14]张洁夫,戚存扣,浦惠明,等.甘蓝型油菜主要脂肪酸组成的QTL定位[J].作物学报,2008,34(1):54-60.
[15]钦洁.甘蓝型油菜种子油酸和亚麻酸含量的遗传及其基因定位[D].武汉:华中农业大学,2009.
[16]陈伟.甘蓝型油菜种子高油酸和低亚麻酸含量的分子标记辅助选择[D].武汉:华中农业大学,2009.
[17]杨燕宇.甘蓝型油菜SSR分子标记图谱构建和油酸含量QTL定位[D].长沙:湖南农业大学,2011.
[18]杨庆勇.油菜种子高油酸低亚麻酸的遗传控制及等位基因特异标记开发[D].武汉:华中农业大学,2012.
[19]QIU D,MORGAN C,SHI J,et al. A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content.Theoretical and Applied Genetics,2006,114(1):67-80.
[20]HARPER A L,TRICK M,HIGGINS J,et al. Associative transcriptomics of traits in the polyploid crop species Brassica napus[J]. Nature Biotechnology,2012,30:798-802.
[21] LI F,CHEN B,XU K,et al. Genome-wide association study dissects the genetic architecture of seed weight and seed quality in rapeseed(Brassica napus L.)[J]. DNA Research,2014,21:355-367.
[22]QU C,LI S,DUAN X,et al. Identification of candidate genes for seed glucosinolate content using association mapping in Brassica napus L.Genes,2015,6:1215-1229.
[23]QU C,JIA L,FU F,et al. Genome-wide association mapping and identification of candidate genes for fatty acid composition in Brassica napus L.using SNP markers. BMC Genomics,2017,18:232-248.
[24]BARRET P,DELOURME R,RENARD M,et al. A rapeseed FAE1 gene is linked to the E1 locus associated with variation in the content of erucic acid[J]. Theoretical and Applied Genetics,1998,96(2):177-186.
[25]HAN J X,LUHS W,SONNTAG K,et al. Functional characterization of beta-ketoacyl-CoA synthase genes from Brassica napus L[J]. Plant Molecular Biology,2001,46(2):229-239.
[26]WU L,JIA Y L,WU G,et al. Molecular evidence for blocking erucic acid synthesis in rapeseed(Brassica napus L.)by a two-base-pair deletion in FAE1(fatty acid elongase 1)[J]. Journal of Integrative Agriculture,2015,14(7):1251-1260.
[27]WU G,WU Y,XIAO L,et al. Zero erucic acid trait of rapeseed(Brassica napus L.)results from a deletion of four base pairs in the fatty acid elongase 1 gene[J]. Theoretical and Applied Genetics,2008,116(4):491-499.
[28]WANG N,SHI L,TIAN F,et al. Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents[J]. BMC Plant Biol,2010,10:137-147.
[29]CHALHOUB B,DENOEUD F,LIU S,et al. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome[J]. Science,2014,345(6199),950-953.
[30]张洁夫,戚存扣,浦惠明,等.甘蓝型油菜芥酸含量的遗传与QTL定位[J].江苏农业学报,2008,24(1):22-28.
[31]林兵.甘蓝型油菜A8连锁群种子含油量QTL簇解析及连锁累赘分析[D].武汉:华中农业大学,2010.
[32]汤天泽.工业专用特高芥酸甘蓝型油菜芥酸含量的遗传、杂种优势、分子标记及关键基因克隆分析[D].成都:四川农业大学,2016.
[33]KUSHAD M M,BROWN,A F,KURILICH A C,et al. Variation of glucosinolates in vegetable crops of Brassica oleracea[J]. Journal of Agricultural&Food Chemistry,1999,47(4):1541-1548.
[34]KJAER A. Naturally derived isothiocyanates(mustard oils)and their parent glucosides[M]//Fortschritte der Chemie organischer Naturstoffe.Progress in the Chemistry of Organic Natural Products. Progrés Dans la Chimie des Substances Organiques Naturelles. Springer Vienna,1960,18:122-176.
[35]BELL J M. Factors affecting the nutritional value of canola meal:a review[J]. Canadian Veterinary Journal La Revue Veterinaire Canadienne,1993,73(4):689-697.
[36]HOWELL P M,SHARPE A G,LYDIATE D J. Homoeologous loci control the accumulation of seed glucosinolates in oilseed rape(Brassica napus)[J]. Genome,2003,46(3):454-460.
[37]LU G,HARPER A L,TRICK M,et al. Associative transcriptomics study dissects the genetic architecture of seed glucosinolate content in Brassica napus[J]. DNA Research,2014,21:613-625.
[38]GIGOLASHVILI T,YATUSEVICH R,BERGER B,et al. The R2R3-MYB transcription factor HAG1/MYB28 is a regulator of methionine-derived glucosinolate biosynthesis in Arabidopsis thaliana[J]. Plant Journal,2007,51(2),247-261.
[39]WANG B,WU Z,LI Z,et al. Dissection of the genetic architecture of three seed-quality traits and consequences for breeding in Brassica napus[J]. Plant Biotechnol Journal,2018,16(7):1336-1348.
[40]MARSCHALEK R,BECKER H C,ECKE W. QTL effects for seed glucosinolate content in BC3families of rapeseed(Brassica napus L.)segregating for single QTL only[C]. Proceedings of 11thInternational Rapeseed Congress,Copenhagen Denmark,2003,1:260-262.
[41]LIONNETON E,AUBERT G,OCHATT S. A candidate gene approach identified loci involved in the glucosinolate biosynthesis in mustard(Brassica juncea)[C]. Proceedings of 11thInternational Rapeseed Congress,Copenhagen Denmark,2003,1:169-171.
[42]RIPLEY V L,RANEY J P,RAKOW G. AFLP markers linked to seed quality traits in Brassica juncea[C]. Proceedings of 11thInternational Rapeseed Congress,Copenhagen Denmark,2003,1:166-168.
[43]刘梓谕.高含油量甘蓝型油菜新品系指纹图谱的构建及与含油量连锁标记的筛选[D].武汉:中国农业科学院油料作物研究所,2008.
[2]ZHAO J Y,BECKER H C,ZHANG D Q,et al. Conditional QTL mapping of oil content in rapeseed with respect to protein content and traits related to plant development and grain yield[J]. Theoretical and Applied Genetics,2006,113(1):33-38.
[3]张宁.用分子标记技术初步解析甘蓝型油菜种子含油量QTL簇qc O. C2[D].武汉:华中农业大学,2009.
[4]郭世星.甘蓝型油菜含油量等重要品质性状的遗传分析与QTL定位[D].成都:四川农业大学,2010.
[5]WANG X,WANG H,LONG Y,et al. Identification of QTLs associated with oil content in a high-oil Brassica napus cultivar and construction of a high-density consensus map for QTLs comparison in B. napus[J]. PLOS ONE,2013,8(12):e80569.
[6]TEH L,MOLLERS C. Genetic variation and inheritance of phytosterol and oil content in a doubled haploid population derived from the winter oilseed rape Sansibar×Oase cross[J]. Theoretical and Applied Genetics,2016,129(1):181-199.
[7]SUN M Y,HUA W,LIU J,et al. Design of new genome-and gene-sourced primers and identification of QTL for seed oil content in a specially high-oil Brassica napus cultivar[J]. PLOS ONE,2012,7(10):e47037.
[8]RABOANATAHIRY N,CHAO H B,GUO L X,et al. Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus[J]. BMC Genomics,2017,18:776-790.
[9]孔祥瑞.必需微量元素的营养生理及临床意义[M].合肥:安徽科学出版社,1982.
[10]SHEN J H,MA Q,SHEN S R,et al. Effect ofα-linolenic acid on streptozotocin-induced diabetic retinopathy indices in vivo[J]. Archives of Medical Research,2013,44(7):514-520.
[11]潘勇.α-亚麻酸在体内外的吸收和转化为长链多不饱和脂肪酸效率的研究[D].无锡:江南大学,2016.
[12]HU X,SULLIVAN-GILBERT M,GUPTA M,et al. Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canol(Brassica napus L.)[J]. Theoretical and Applied Genetics,2006,113(3):497-507.
[13]JAVIDFAR F,RIPLEY V L,ROSLINSKY V. Identification of molecular markers associated with oleic and linolenic acid in spring oilseed rape(Brassica napus)[J]. Plant Breeding,2006,125(1):65-71.
[14]张洁夫,戚存扣,浦惠明,等.甘蓝型油菜主要脂肪酸组成的QTL定位[J].作物学报,2008,34(1):54-60.
[15]钦洁.甘蓝型油菜种子油酸和亚麻酸含量的遗传及其基因定位[D].武汉:华中农业大学,2009.
[16]陈伟.甘蓝型油菜种子高油酸和低亚麻酸含量的分子标记辅助选择[D].武汉:华中农业大学,2009.
[17]杨燕宇.甘蓝型油菜SSR分子标记图谱构建和油酸含量QTL定位[D].长沙:湖南农业大学,2011.
[18]杨庆勇.油菜种子高油酸低亚麻酸的遗传控制及等位基因特异标记开发[D].武汉:华中农业大学,2012.
[19]QIU D,MORGAN C,SHI J,et al. A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content.Theoretical and Applied Genetics,2006,114(1):67-80.
[20]HARPER A L,TRICK M,HIGGINS J,et al. Associative transcriptomics of traits in the polyploid crop species Brassica napus[J]. Nature Biotechnology,2012,30:798-802.
[21] LI F,CHEN B,XU K,et al. Genome-wide association study dissects the genetic architecture of seed weight and seed quality in rapeseed(Brassica napus L.)[J]. DNA Research,2014,21:355-367.
[22]QU C,LI S,DUAN X,et al. Identification of candidate genes for seed glucosinolate content using association mapping in Brassica napus L.Genes,2015,6:1215-1229.
[23]QU C,JIA L,FU F,et al. Genome-wide association mapping and identification of candidate genes for fatty acid composition in Brassica napus L.using SNP markers. BMC Genomics,2017,18:232-248.
[24]BARRET P,DELOURME R,RENARD M,et al. A rapeseed FAE1 gene is linked to the E1 locus associated with variation in the content of erucic acid[J]. Theoretical and Applied Genetics,1998,96(2):177-186.
[25]HAN J X,LUHS W,SONNTAG K,et al. Functional characterization of beta-ketoacyl-CoA synthase genes from Brassica napus L[J]. Plant Molecular Biology,2001,46(2):229-239.
[26]WU L,JIA Y L,WU G,et al. Molecular evidence for blocking erucic acid synthesis in rapeseed(Brassica napus L.)by a two-base-pair deletion in FAE1(fatty acid elongase 1)[J]. Journal of Integrative Agriculture,2015,14(7):1251-1260.
[27]WU G,WU Y,XIAO L,et al. Zero erucic acid trait of rapeseed(Brassica napus L.)results from a deletion of four base pairs in the fatty acid elongase 1 gene[J]. Theoretical and Applied Genetics,2008,116(4):491-499.
[28]WANG N,SHI L,TIAN F,et al. Assessment of FAE1 polymorphisms in three Brassica species using EcoTILLING and their association with differences in seed erucic acid contents[J]. BMC Plant Biol,2010,10:137-147.
[29]CHALHOUB B,DENOEUD F,LIU S,et al. Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome[J]. Science,2014,345(6199),950-953.
[30]张洁夫,戚存扣,浦惠明,等.甘蓝型油菜芥酸含量的遗传与QTL定位[J].江苏农业学报,2008,24(1):22-28.
[31]林兵.甘蓝型油菜A8连锁群种子含油量QTL簇解析及连锁累赘分析[D].武汉:华中农业大学,2010.
[32]汤天泽.工业专用特高芥酸甘蓝型油菜芥酸含量的遗传、杂种优势、分子标记及关键基因克隆分析[D].成都:四川农业大学,2016.
[33]KUSHAD M M,BROWN,A F,KURILICH A C,et al. Variation of glucosinolates in vegetable crops of Brassica oleracea[J]. Journal of Agricultural&Food Chemistry,1999,47(4):1541-1548.
[34]KJAER A. Naturally derived isothiocyanates(mustard oils)and their parent glucosides[M]//Fortschritte der Chemie organischer Naturstoffe.Progress in the Chemistry of Organic Natural Products. Progrés Dans la Chimie des Substances Organiques Naturelles. Springer Vienna,1960,18:122-176.
[35]BELL J M. Factors affecting the nutritional value of canola meal:a review[J]. Canadian Veterinary Journal La Revue Veterinaire Canadienne,1993,73(4):689-697.
[36]HOWELL P M,SHARPE A G,LYDIATE D J. Homoeologous loci control the accumulation of seed glucosinolates in oilseed rape(Brassica napus)[J]. Genome,2003,46(3):454-460.
[37]LU G,HARPER A L,TRICK M,et al. Associative transcriptomics study dissects the genetic architecture of seed glucosinolate content in Brassica napus[J]. DNA Research,2014,21:613-625.
[38]GIGOLASHVILI T,YATUSEVICH R,BERGER B,et al. The R2R3-MYB transcription factor HAG1/MYB28 is a regulator of methionine-derived glucosinolate biosynthesis in Arabidopsis thaliana[J]. Plant Journal,2007,51(2),247-261.
[39]WANG B,WU Z,LI Z,et al. Dissection of the genetic architecture of three seed-quality traits and consequences for breeding in Brassica napus[J]. Plant Biotechnol Journal,2018,16(7):1336-1348.
[40]MARSCHALEK R,BECKER H C,ECKE W. QTL effects for seed glucosinolate content in BC3families of rapeseed(Brassica napus L.)segregating for single QTL only[C]. Proceedings of 11thInternational Rapeseed Congress,Copenhagen Denmark,2003,1:260-262.
[41]LIONNETON E,AUBERT G,OCHATT S. A candidate gene approach identified loci involved in the glucosinolate biosynthesis in mustard(Brassica juncea)[C]. Proceedings of 11thInternational Rapeseed Congress,Copenhagen Denmark,2003,1:169-171.
[42]RIPLEY V L,RANEY J P,RAKOW G. AFLP markers linked to seed quality traits in Brassica juncea[C]. Proceedings of 11thInternational Rapeseed Congress,Copenhagen Denmark,2003,1:166-168.
[43]刘梓谕.高含油量甘蓝型油菜新品系指纹图谱的构建及与含油量连锁标记的筛选[D].武汉:中国农业科学院油料作物研究所,2008.