灯盏生脉胶囊对短暂性脑缺血发作小鼠认知及血糖和血脂水平的影响
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摘要
目的建立短暂性脑缺血发作(TIA)小鼠模型,探讨灯盏生脉胶囊(DZSM)对短暂性脑缺血发作小鼠认知及血糖血脂水平的影响。方法选用雄性昆明小鼠,采用随机方法分组。采用尾静脉注射t-BHP及低氧方法成功制备了TIA样发作小鼠模型,分别给予不同浓度的DZSM灌胃治疗,观察小鼠病死率,采用Morris水迷宫行为实验法和跳台实验法检测小鼠认知功能,生化分析法测各组小鼠血糖血脂水平。结果灯盏生脉胶囊可降低TIA小鼠的病死率;改善小鼠的定位航行能力、跳台实验潜伏期和错误次数等认知功能障碍;对TIA小鼠空腹血糖、血清总胆固醇、甘油三酯无明显影响。结论灯盏生脉胶囊灯盏生脉胶囊可以改善短暂性脑缺血发作小鼠认知功能且不影响致死率及血糖血脂的水平。
Objective To establish mouse model of transient ischemic attack(TIA)and explore the effects of DZSM on brain cognition function,blood glucose and blood lipid level for mouse model of transient ischemic attack.Methods The TIA mice model were established successfully by injecting t-BHP and living in the hypoxia situation,and the intragastric injection with different doses of DZSM was performed,the mortality rate of mice were counted,brain cognitive function was detected by Morris water maze test and step-down test,the level of the blood lipid and blood glucose of mice were measured by biochemical analysis method. Results DZSM could reduce the mortality of TIA mice,improve the positioning navigation ability,step-down test latency and error times,and have no significant influence on fasting blood glucose,serum total cholesterol and triglyceride in TIA mice. Conclusion DZSM could improve the brain cognitive function of mice with transient ischemic attack,and does not affect the mortality rate and blood glucose and blood lipid levels.
Objective To establish mouse model of transient ischemic attack(TIA)and explore the effects of DZSM on brain cognition function,blood glucose and blood lipid level for mouse model of transient ischemic attack.Methods The TIA mice model were established successfully by injecting t-BHP and living in the hypoxia situation,and the intragastric injection with different doses of DZSM was performed,the mortality rate of mice were counted,brain cognitive function was detected by Morris water maze test and step-down test,the level of the blood lipid and blood glucose of mice were measured by biochemical analysis method. Results DZSM could reduce the mortality of TIA mice,improve the positioning navigation ability,step-down test latency and error times,and have no significant influence on fasting blood glucose,serum total cholesterol and triglyceride in TIA mice. Conclusion DZSM could improve the brain cognitive function of mice with transient ischemic attack,and does not affect the mortality rate and blood glucose and blood lipid levels.
引文
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[11]茆华武,丁立东.低分子肝系治疗频发的短暂性脑缺血发作35例效果观察[J].南通医学院学报,2004,24(2):211-211,213.
[2]刘鸣,谢鹏.神经病学[M].北京:人民卫生出版社,2008:38-40.
[3]贾建平,崔丽英,王伟,等.神经病学[M].北京:人民卫生出版社,2008:173.
[4]施琴,刘涛,许晓春.郭东风短暂性脑缺血发作后认知功能损害的相关性研究[J].南通大学学报(医学版)2010:30(2)109-111.
[5]张卫东.灯盏细辛的化学成分[J].中国医药工业杂志,1998,29(11):49-50.
[6]陈利英.灯盏细辛注射液治疗缺血性脑血管病的临床观察[J].中华综合医学杂志,2003,5(11):32-33.
[7]黄晓芸,丁燕晶,方浩威.灯盏细辛注射液治疗缺血性脑血管病的研究[J].现代中西医结合杂志,2005,14(15):1964-1965.
[8]Bradvik B,Sonesson B,Holtas S.Spatial impairment following right hemisphere transient ischaemic attacks in patients without carotid artery stenosis[J].Acta Neurol Scand,1989,80(5):411-418.
[9]Bisschops RH,Kappelle LJ,Mali WP,et al.Hemodynamic and metabolic changes in transient ischemic attack patients:a magnetic resonance angiography and 1H-magnetic resonance spectroscopy study performed within 3 days of onset of a transient ischemic attack[J].Stroke,2002,33(1):110-115.
[10]Martí-Fàbregas JA,Catafau AM,MaríC,et al.Cerebral perfusion and haemodynamics measured by SPET in symptom free patients with transient ischaemic attack:clinical implications[J].Eur J Nucl Med,2001,28(12):1828-1835.
[11]茆华武,丁立东.低分子肝系治疗频发的短暂性脑缺血发作35例效果观察[J].南通医学院学报,2004,24(2):211-211,213.