广东汉族人群HDAC9基因单核苷酸多态性位点rs2240419与缺血性卒中及其中医证候易感性的关联性研究
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摘要
目的探讨广东汉族人群蛋白去乙酰化酶9(HDAC9)基因单核苷酸多态性位点rs2240419与缺血性卒中(IS)及其中医证候易感性的关系。方法采用前瞻性研究方法 ,运用Sanger基因分型技术对外周静脉血中HDAC9基因位点rs2240419多态性进行基因分型,并通过自行设计的研究对象观察表(CRF表)进行记录后建立数据库,最后导入SPSS 17.0软件进行统计分析。结果①共纳入184例缺血性卒中患者(IS组)及116例健康人(对照组);②rs2240419位点共检测出CC、CT、TT 3种基因型,基因型分布均符合Hardy-Weinberg平衡定律(P=0.936);③对IS组研究对象进行辨别证候,风证141例,火证13例,痰证121例,血瘀证149例,气虚证82例;④HDAC9基因位点rs2240419的多态性与缺血性卒中的关联性差异无显著性(P>0.05);⑤HDAC9基因位点rs2240419的多态性与中医证候易感性(风证、痰证、血瘀证)的关联差异无显著性(P>0.05)。结论广东汉族人群HDAC9基因SNP位点rs2240419可能与缺血性卒中的发病及其中医证候易感性无关,但是并不能据此就明确否认该基因位点的多态性与缺血性卒中及其中医证候的易感性不存在关联性。不排除是本研究与所参考的研究在研究对象、干扰因素控制等方面的研究方案不同,或者本研究自身存在的局限与不足导致阴性结果的产生,仍然需要更多大样本的研究来进一步明确。
引文
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[17]徐晶雪,车显宏,王洋.高同型半胱氨酸血症与脑梗死的关系探讨[J].中国医刊,2015,50(6):73-75.
[18]王翅遥,张卫泽.ABC评分法在非瓣膜病心房颤动患者预测卒中风险评估中的作用[J].中国临床医生杂志,2017,45(12):38-40.
[2] Feigin VL, Lawes CM, Bennett DA, et al. Worldwide stroke incidence and early case fatality reported in 56 population-based studies:a systematic review[J]. Lancet Neurology, 2009,8(4):355.
[3]中华人民共和国国家统计局.中国统计年鉴[M].北京:中国统计出版社,2016.
[4] Baird AE. Genetics and genomics of stroke:novel approaches[J]. J Am Coll Cardiol 2010. 56(4):245-253.
[5] Liu YJ, Liu XG, Wang L,et al. Genome-wide association scans identified CTNNBL1 as a novel gene for obesity[J]. Hum Mol Genet2008, 17(12):1803-1813.
[6] Bellenguez C, Bevan S, Gschwendtner A, et al. Genome-wide association study identifies a variant in HDAC9 associated with large vessel ischemic stroke[J]. Nature Genetics, 2012, 44(3):328-33.
[7] Han Y, Sun W, Wang L, et al. HDAC9 gene is associated with stroke risk in a Chinese population[J]. Experimental Biology&Medicine, 2013, 238(7):842-847.
[8]王新德.各类脑血管疾病诊断要点[J].中华神经科杂志,1996(6):379-380.
[9]佚名.中风病诊断与疗效评定标准(试行)[J].北京中医药大学学报,1996,19(1):55-56.
[10]国家中医药管理局脑病急症科研组.中风病辨证诊断标准(试行)[J].北京中医药大学学报,1996,19(1):55-56.
[11] Mejat A,Ramond F, Basselduby R, et al. Histone deacetylase 9couples neuronal activity to muscle chromatin acetylation and gene expression[J]. Nature Neuroscience,2005,8(3):313-321.
[12] Kaluza D, Kroll J, Gesierich S, et al. Histone deacetylase 9 promotes angiogenesis by targeting the antiangiogenic microRNA-17-92cluster in endothelial cells[J]. Arteriosclerosis Thrombosis&Vascular Biology, 2013, 33(3):533.
[13] Markus HS, Makela KM, Bevan S,et al. Evidence HDAC9 genetic variant associated with ischaemic stroke increases risk via promoting carotid atherosclerosis[J]. Stroke,2013,44(5):1220-1225.
[14]贺敏.乙酰化表观遗传调控分子HDAC4/5在缺血性脑损伤中的作用及机制[D].济南:山东大学,2012.
[15] Traylor M, Farrall M, Holliday EG, et al. Genetic risk factors for ischaemic stroke and its subtypes(the METASTROKE Collaboration):a meta-analysis of genome-wide association studies[J]. Lancet Neurology, 2012, 11(11):951-962.
[16]柳维林,林云娇,陶静,等.HDAC9基因SNPs与大动脉粥样硬化缺血性卒中的相关性研究[J].中国卒中杂志,2016(4):269-276.
[17]徐晶雪,车显宏,王洋.高同型半胱氨酸血症与脑梗死的关系探讨[J].中国医刊,2015,50(6):73-75.
[18]王翅遥,张卫泽.ABC评分法在非瓣膜病心房颤动患者预测卒中风险评估中的作用[J].中国临床医生杂志,2017,45(12):38-40.