植烟密度对烤烟“高温逼熟”现象的影响
|
摘要
为了解不同植烟密度对烤烟"高温逼熟"现象的影响,设计五种不同植烟密度:13 500株/hm~2、15 000株/hm~2、16 500株/hm~2、17 250株/hm~2和18 000株/hm~2,研究不同植烟密度对烟株田间微气候、"高温逼熟"发生率、抗氧化酶活性、烤后烟叶化学成分和经济性状的影响。结果表明,15 000株/hm~2种植密度下"高温逼熟"发生率最低,该处理下田间微气候因子与"高温逼熟"发生率没有显著相关性,说明该处理下的"高温逼熟"发生率与种植密度无关。烟叶中的过氧化物酶(peroxidase,POD)、多酚氧化酶(polyphenol oxidase,PPO)、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)均为15 000株/hm~2处理下活性较大,说明15 000株/hm~2处理诱导POD、PPO、SOD和CAT活性提高,增强烟株抗逆性。15 000株/hm~2种植密度下,烟叶化学成分协调性较为适宜,经济性状较好。因此,相对于其他处理,15 000株/hm~2植烟密度有利于提高与抗性有关的酶活性,降低"高温逼熟"发生率,提高烟叶的产量和质量。
In order to determine the influence of bacco plant density on the phenomenon of "high temperature induced maturity",this paper designed five different planting densities: 13 500 plants/hm~2,15 000 plants/hm~2,16 500 plants/hm~2,17 250 plants/hm~2,and 18 000 plants/hm~2; and studied the field microclimate,the incidence rate of "high temperature induced maturity",the activity of antioxidant enzymes,the chemical component and the economic characteristics with different planting densities. The results showed that the incidence rate of "high temperature induced maturity"under the treatment of 15 000 plants/hm~2 was the lowest; there was no significant correlation between field microclimate and incidence rate of "high temperature induced maturity" under this treatment,which suggested the planting density did not affect the incidence of "high temperature induced maturity" with planting density of 15 000 plants/hm~2. The activities of peroxidase( POD), polyphenol oxidase( PPO), superoxide dismutase( SOD),and catalase( CAT) in tobacco leaf were more active under the treatment of 15 000 plants/hm~2 than those under other treatments. Under the planting density of 15 000 plants/hm~2,the chemical composition of tobacco leaf was suitable and its economic characteristics were the best. Therefore,compared with other treatments,the planting density of 15 000 plants/hm~2 was conducive to improve the peroxidases activity response to stress,reduce the incidence of "high temperature induced maturity",and improve the yield and quality of flue-cured tobacco leaves.
In order to determine the influence of bacco plant density on the phenomenon of "high temperature induced maturity",this paper designed five different planting densities: 13 500 plants/hm~2,15 000 plants/hm~2,16 500 plants/hm~2,17 250 plants/hm~2,and 18 000 plants/hm~2; and studied the field microclimate,the incidence rate of "high temperature induced maturity",the activity of antioxidant enzymes,the chemical component and the economic characteristics with different planting densities. The results showed that the incidence rate of "high temperature induced maturity"under the treatment of 15 000 plants/hm~2 was the lowest; there was no significant correlation between field microclimate and incidence rate of "high temperature induced maturity" under this treatment,which suggested the planting density did not affect the incidence of "high temperature induced maturity" with planting density of 15 000 plants/hm~2. The activities of peroxidase( POD), polyphenol oxidase( PPO), superoxide dismutase( SOD),and catalase( CAT) in tobacco leaf were more active under the treatment of 15 000 plants/hm~2 than those under other treatments. Under the planting density of 15 000 plants/hm~2,the chemical composition of tobacco leaf was suitable and its economic characteristics were the best. Therefore,compared with other treatments,the planting density of 15 000 plants/hm~2 was conducive to improve the peroxidases activity response to stress,reduce the incidence of "high temperature induced maturity",and improve the yield and quality of flue-cured tobacco leaves.
引文
[1]孔萍,殷剑敏,谢佳杏,等.高温逼熟对江西省双季早稻产量的影响[J].中国农业大学学报,2015,20(4):57-65.Kong P,Yin J M,Xie J X,et al..Study on the yield of early rice by heat-forced maturing in Jiangxi province[J].J.China Agric.Univ.,2015,20(4):57-65.
[2]赵东杰,赵喆,毛亚博,等.抚州地区高温逼熟烟叶化学成分与致香成分的相关性[J].山西农业科学,2017,45(9):1420-1425.Zhao D J,Zhao Z,Mao Y B,et al..Correlation analysis between chemical composition and aroma components of high temperature induced maturity tobacco leaves in Fuzhou area[J].Shanxi Agric.Sci.,2017,45(9):1420-1425.
[3]冯敏玉,祝必琴,雷俊,等.南昌高温逼熟发生规律及其对早稻产量的影响[J].中国农业气象,2014,35(3):287-292.Feng M Y,Zhu B Q,Lei J,et al..Characteristics of hightemperature forced maturity disaster and it's impacts on early rice in Nanchang area[J].Chin.J.Agrometeorol.,2014,35(3):287-292.
[4]李琦.烤烟优劣质年的气象条件分析[J].安徽农业科学,1997(2):32-35.Li Q.Analysis of meteorological conditions of flue-cured tobacco quality[J].J.Anhui Agric.Sci.,1997(2):32-35.
[5]黄国文,陈良碧.高温对烟叶品质的影响[J].生命科学研究,2002,6(4):362-366.Huang G W,Chen L B.The influence of high temperature on the quality of tobacco leaves[J].Life Sci.Res.,2002,6(4):362-366.
[6]王小青.高温胁迫下烟草-TMV互作中的防御酶活性变化[D].郑州:河南农业大学,硕士学位论文,2006.Wang X Q.Changed activities of defense-related enzymes in Nicotiana-TMV interaction under high-temperature stress[D].Zhengzhou:Henan Agriculture University,Master Dissertation,2006.
[7]陆德彪,骆耀平,童启庆.过氧化氢酶在干旱胁迫中的活性变化及其与茶树抗旱性关系[J].浙江大学学报(农业与生命科学版),1992(S1):63-68.Lu D B,Luo Y P,Tong Q Q.The changes of CAT activity during drought stress with relation to drought resistance of tea plant[J].J.Zhejiang Univ.(Agric.Life Sci.),1992(S1):63-68.
[8]杨通隆,吴峰,李洪勋,等.不同植烟密度对烟叶产质量的影响[J].安徽农业科学,2008,36(33):14617-14618.Yang T L,Wu F,Li H X,et al..Effect of different planting density on the quality of tobacco leaves[J].Anhui Agric.Sci.,2008,36(33):14617-14618.
[9]李正,敬海霞,刘冲.不同植烟密度对旺长期烟田微生态环境及烤后烟产值效益的影响[A].见:中国烟草学会2014年学术年会入选论文摘要汇编[C].2014.
[10]王伦梅,潘锋,王定斌,等.不同移栽密度对云烟85生长及产量和品质的影响[J].天津农业科学,2010,16(6):33-35.Wang L M,Pan F,Wang D B,et al..Effects of different density on growth,yield and quality of Yunyan 85[J].Tianjin Agric.Sci.,2010,16(6):33-35.
[11]郭瑞鸽,刘文英,蔡哲,等.江西省高温逼熟灾害特征分析[J].中国农学通报,2015,31(11):268-273.Guo R G,Liu W Y,Cai Z,et al..Characteristic analysis of high-temperature forced maturity disaster in Jiangxi province[J].China Agric.Bull.,2015,31(11):268-273.
[12]赵东杰,赵喆,毛亚博,等.高温热害气象指标对成熟期烟叶的影响评估[J].中国烟草科学,2017,38(5):62-68.Zhao D J,Zhao Z,Mao Y B,et al..Assessment on meteorological indexes of high temperature damage to tobacco leaves at maturity stage[J].China Tobacco Sci.,2017,38(5):62-68.
[13]王子腾.湘南烤烟“高温逼熟”指标监测及光温对烤烟光合生理的影响[D].郑州:郑州大学,硕士学位论文,2017.Wang Z T.The monitoring of“heat-forced maturity”index in Xiangnan and the response of heat and irradiance stress on photosynthetic physiology in tobacco[D].Zhengzhou:Zhengzhou University,Master Dissertation,2017.
[14]徐朗莱,叶茂炳.过氧化物酶活力连续记录测定法[J].南京农业大学学报,1989,12(3):82-83.Xu L L,Ye M B.Continuous recording of peroxidase activity[J].J.Nanjing Agric.U.,1989,12(3):82-83.
[15]郝再斌.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004,116-117.Hao Z B.Plant physiology experiment[M].Harbin:Harbin Industrial University Press,2004,116-117.
[16]李仕飞,刘世同,周建平,等.分光光度法测定植物过氧化氢酶活性的研究[J].安徽农学通报,2007,13(2):72-73.Li S F,Liu S T,Zhou J P,et al..Spectrophotometric determination of catalase activity in plants[J].Anhui Agric.Bull.,2007,13(2):72-73.
[17]王卫康.《烤烟》国标中分级因素的概念及把握[J].烟草科技,2004(5):44-48.Wang W K.Concept and control of grading factors in national standard-flue-cured tobacco[J].Tobacco Sci.Technol.,2004(5):44-48.
[18]程传玲,唐琦,汪文良,等.烤烟常规化学成分与感官质量的典型相关分析[J].贵州农业科学,2011,39(1):59-61.Cheng C L,Tang Q Wang W L,et al..Canonical correlation between conventional chemical composition and sensory quality in flue-cured tobacco[J].Guizhou Agric.Sci.,2011,39(1):59-61.
[19]刘国顺.烟草栽培学[M].北京:中国农业出版社,2003,72-74.Liu G S.Tobacco cultivation[M].Beijing:China Agriculture Press,2003,72-74.
[20]陈瑞泰.中国烟草栽培学[M].上海:上海科技出版社,1987.Chen R T.Chinese tobacco culture[M].Shanghai:Shanghai Science&Technology Press,1987.
[21]王剑英,张帮琨,江锡瑜.烟地微气象与规范化栽培[J].烟草科技,1994(05):33-36.Wang J Y,Zhang B K,Jiang X Y.Tobacco micrometeorological and standardized cultivation[J].Tobacco Sci.Technol.,1994(05):33-36.
[22]李海平,朱列书,黄魏魏,等.不同植烟密度对填充型烟叶烟株农艺性状和产量的影响[J].湖南农业科学,2008(3):34-35.Li H P,Zhu L S,Huang W W,et al..Effects of different tobacco density on the agronomic characters and yield of the filled tobacco plant[J].Hunan Agric.Sci.,2008(3):34-35.
[23]朱大恒,张闪闪,莫骏坚,等.光强对湘南烟草生育后期光合及生理特性的影响[J].江苏农业科学,2018,46(8):81-84.Zhu D H,Zhang S S,Mo J J,et al..Effects of light intensity on photosynthesis and physiological characteristics of tobacco in late growth period in southern Hunan[J].Jiangsu Agric.Sci.,2018,46(8):81-84.
[24]谢兆文,王寿昆,林旋,等.高温胁迫对仿刺参抗氧化酶等指标的影响[J].水产科学,2016,35(4):327-333.Xie Z W,Wang S K,Lin X,et al..Effects of high temperature stress on antioxidant enzymes and other indexes of apostichopus japonicus[J].Fisheries Sci.,2016,35(4):327-333.
[25]任彩虹,闫桂琴,郜刚,等.高温胁迫对马铃薯幼苗叶片生理效应的影响[J].中国马铃薯,2007,21(1):5-10.Ren C H,Yan G Q,Gao G,et al..Effects of high temperature stress on physiological effects of potato seedling leaves[J].China Potato J.,2007,21(1):5-10.
[26]杜秀敏,殷文璇,张慧,等.超氧化物歧化酶(SOD)研究进展[J].中国生物工程杂志,2003,23(1):48-50.Du X M,Yin W X,Zhang H,et al..Research progress of superoxide dismutase(SOD)[J].J.China Biotechnol.,2003,23(1):48-50.
[27]朱雪梅,柯永培,邵继荣,等.高温胁迫对重穗型水稻品种叶片活性氧代谢的影响[J].种子,2005,24(3):25-27.Zhu X M,Ke Y P,Shao J R,et al..Effects of high temperature stress on activated oxygen metabolism of the leaves of the heavy panicle type of medium India hybrid rice[J].Seed,2005,24(3):25-27.
[28]孙胜楠.设施黄瓜对高温胁迫的响应与适应[D].山东泰安:山东农业大学,硕士学位论文,2017.Sun S N.Response and adaptation of protected cucumber to high temperature stress[D].Shandong Taian:Shandong Agriculture University,Master Dissertation,2017.
[29]Lee B R,Kim K Y,Jung W J,et al..Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover(Trifolium repens L.)[J].J.Exp.Bot.,2007,58(6):1271.
[30]Sofo A,Dichio B,Xiloyannis C,et al..Antioxidant defences in olive trees during drought stress:Changes in activity of some antioxidant enzymes[J].Funct.Plant Biol.,2005,32(1):351-364.
[31]齐玉鑫,李文滨,任海祥,等.施肥量和种植密度对大豆牡丰7号保护性酶活性的影响[J].黑龙江农业科学,2012(5):47-49.Qi Y X,Li W B,Ren H X,et al..Effects of fertilizer application and planting density on the activity of protective enzyme of soybean Mufeng No.7[J].Heilongjiang Agric.Sci.,2012(5):47-49.
[32]陈顺辉,李文卿,江荣风,等.施氮量对烤烟产量和品质的影响[J].中国烟草学报,2003,9(Z1):36-40.Chen S H,Li W Q,Jiang R F,et al..Effects of nitrogen application on yield and quality of flue-cured tobacco[J].China Tobacco Sci.,2003,9(Z1):36-40.
[33]闫玉秋,方智勇.试论烟草中烟碱含量及其调节因素[J].烟草科技,1996,50(6):31-34.Yan Y Q,Fang Z Y.Try to talk about nicotine content in tobacco and its control factors[J].Tobacco Sci.Technol.,1996,50(6):31-34.
[34]陈延玲,吴秋平,陈晓超,等.不同耐密性玉米品种的根系生长及其对种植密度的响应[J].植物营养与肥料学报,2012,18(1):52-59.Chen Y L,Wu Q P,Chen X C,et al..Different tightness of corn resistant varieties of root growth and its response to planting density[J].J.Plant Nutr.Fertilizer,2012,18(1):52-59.
[35]宋日,刘利,吴春胜,等.根系生长空间对玉米生长和养分吸收的影响[J].西北农林科技大学学报,2009,37(6):58-64.Song R,Liu L,Wu C S,et al..The effect of root growth space on maize growth and nutrient absorption[J].J.Northwest A.F.U.,2009,37(6):58-64.
[36]蒋士东,周冀衡,李强,等.不同处理对烟叶经济性状及化学协调性的影响[J].中国农业科技导报,2017,19(11):100-107.Jiang S D,Zhou J H,Li Q,et al..Effects of different treatments on economic characters and chemical coordination of flue cured tobacco[J].J.Agric.Sci.Technol.,2017,19(11):100-107.
[37]汪丽,刘雷,杨文钰,等.种植密度与施钾量对烤烟品质的影响[J].华北农学报,2007,22(增):106-110.Wang L,Liu L,Yang W Y,et al..Effects of plant population and K application level on the quality of leaves of flue-cured tobacco[J].Acta Agric.Boreali-Sin.,2007,22(Suppl.):106-110.
[38]王程栋,王树声,刘新民,等.曲靖烟区土壤化学性状及海拔对烟叶钾含量的影响[J].中国烟草科学,2013,34(4):25-29.Wang C D,Wang S S,Liu X M,et al..Effects of soil chemical properties and altitude on K content in flue-cured tobacco leaves in Qujing area[J].China Tobacco Sci.,2013,34(4):25-29.
[39]吴家昶,李军营,杨宇虹,等.种植密度对津巴布韦引进品种KRK26烟叶产量质量和化学成分的影响[J].西南农业学报,2011,24(1):38-42.Wu J X,Li J Y,Yang Y H,et al..Effect of different planting density on tobacco leaf yield,quality and chemical components for flue-cured tobacco variety KRK26 introduced from Zimbabwe[J].Southwest China J.Agric.Sci.,2011,24(1):38-42.
[2]赵东杰,赵喆,毛亚博,等.抚州地区高温逼熟烟叶化学成分与致香成分的相关性[J].山西农业科学,2017,45(9):1420-1425.Zhao D J,Zhao Z,Mao Y B,et al..Correlation analysis between chemical composition and aroma components of high temperature induced maturity tobacco leaves in Fuzhou area[J].Shanxi Agric.Sci.,2017,45(9):1420-1425.
[3]冯敏玉,祝必琴,雷俊,等.南昌高温逼熟发生规律及其对早稻产量的影响[J].中国农业气象,2014,35(3):287-292.Feng M Y,Zhu B Q,Lei J,et al..Characteristics of hightemperature forced maturity disaster and it's impacts on early rice in Nanchang area[J].Chin.J.Agrometeorol.,2014,35(3):287-292.
[4]李琦.烤烟优劣质年的气象条件分析[J].安徽农业科学,1997(2):32-35.Li Q.Analysis of meteorological conditions of flue-cured tobacco quality[J].J.Anhui Agric.Sci.,1997(2):32-35.
[5]黄国文,陈良碧.高温对烟叶品质的影响[J].生命科学研究,2002,6(4):362-366.Huang G W,Chen L B.The influence of high temperature on the quality of tobacco leaves[J].Life Sci.Res.,2002,6(4):362-366.
[6]王小青.高温胁迫下烟草-TMV互作中的防御酶活性变化[D].郑州:河南农业大学,硕士学位论文,2006.Wang X Q.Changed activities of defense-related enzymes in Nicotiana-TMV interaction under high-temperature stress[D].Zhengzhou:Henan Agriculture University,Master Dissertation,2006.
[7]陆德彪,骆耀平,童启庆.过氧化氢酶在干旱胁迫中的活性变化及其与茶树抗旱性关系[J].浙江大学学报(农业与生命科学版),1992(S1):63-68.Lu D B,Luo Y P,Tong Q Q.The changes of CAT activity during drought stress with relation to drought resistance of tea plant[J].J.Zhejiang Univ.(Agric.Life Sci.),1992(S1):63-68.
[8]杨通隆,吴峰,李洪勋,等.不同植烟密度对烟叶产质量的影响[J].安徽农业科学,2008,36(33):14617-14618.Yang T L,Wu F,Li H X,et al..Effect of different planting density on the quality of tobacco leaves[J].Anhui Agric.Sci.,2008,36(33):14617-14618.
[9]李正,敬海霞,刘冲.不同植烟密度对旺长期烟田微生态环境及烤后烟产值效益的影响[A].见:中国烟草学会2014年学术年会入选论文摘要汇编[C].2014.
[10]王伦梅,潘锋,王定斌,等.不同移栽密度对云烟85生长及产量和品质的影响[J].天津农业科学,2010,16(6):33-35.Wang L M,Pan F,Wang D B,et al..Effects of different density on growth,yield and quality of Yunyan 85[J].Tianjin Agric.Sci.,2010,16(6):33-35.
[11]郭瑞鸽,刘文英,蔡哲,等.江西省高温逼熟灾害特征分析[J].中国农学通报,2015,31(11):268-273.Guo R G,Liu W Y,Cai Z,et al..Characteristic analysis of high-temperature forced maturity disaster in Jiangxi province[J].China Agric.Bull.,2015,31(11):268-273.
[12]赵东杰,赵喆,毛亚博,等.高温热害气象指标对成熟期烟叶的影响评估[J].中国烟草科学,2017,38(5):62-68.Zhao D J,Zhao Z,Mao Y B,et al..Assessment on meteorological indexes of high temperature damage to tobacco leaves at maturity stage[J].China Tobacco Sci.,2017,38(5):62-68.
[13]王子腾.湘南烤烟“高温逼熟”指标监测及光温对烤烟光合生理的影响[D].郑州:郑州大学,硕士学位论文,2017.Wang Z T.The monitoring of“heat-forced maturity”index in Xiangnan and the response of heat and irradiance stress on photosynthetic physiology in tobacco[D].Zhengzhou:Zhengzhou University,Master Dissertation,2017.
[14]徐朗莱,叶茂炳.过氧化物酶活力连续记录测定法[J].南京农业大学学报,1989,12(3):82-83.Xu L L,Ye M B.Continuous recording of peroxidase activity[J].J.Nanjing Agric.U.,1989,12(3):82-83.
[15]郝再斌.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004,116-117.Hao Z B.Plant physiology experiment[M].Harbin:Harbin Industrial University Press,2004,116-117.
[16]李仕飞,刘世同,周建平,等.分光光度法测定植物过氧化氢酶活性的研究[J].安徽农学通报,2007,13(2):72-73.Li S F,Liu S T,Zhou J P,et al..Spectrophotometric determination of catalase activity in plants[J].Anhui Agric.Bull.,2007,13(2):72-73.
[17]王卫康.《烤烟》国标中分级因素的概念及把握[J].烟草科技,2004(5):44-48.Wang W K.Concept and control of grading factors in national standard-flue-cured tobacco[J].Tobacco Sci.Technol.,2004(5):44-48.
[18]程传玲,唐琦,汪文良,等.烤烟常规化学成分与感官质量的典型相关分析[J].贵州农业科学,2011,39(1):59-61.Cheng C L,Tang Q Wang W L,et al..Canonical correlation between conventional chemical composition and sensory quality in flue-cured tobacco[J].Guizhou Agric.Sci.,2011,39(1):59-61.
[19]刘国顺.烟草栽培学[M].北京:中国农业出版社,2003,72-74.Liu G S.Tobacco cultivation[M].Beijing:China Agriculture Press,2003,72-74.
[20]陈瑞泰.中国烟草栽培学[M].上海:上海科技出版社,1987.Chen R T.Chinese tobacco culture[M].Shanghai:Shanghai Science&Technology Press,1987.
[21]王剑英,张帮琨,江锡瑜.烟地微气象与规范化栽培[J].烟草科技,1994(05):33-36.Wang J Y,Zhang B K,Jiang X Y.Tobacco micrometeorological and standardized cultivation[J].Tobacco Sci.Technol.,1994(05):33-36.
[22]李海平,朱列书,黄魏魏,等.不同植烟密度对填充型烟叶烟株农艺性状和产量的影响[J].湖南农业科学,2008(3):34-35.Li H P,Zhu L S,Huang W W,et al..Effects of different tobacco density on the agronomic characters and yield of the filled tobacco plant[J].Hunan Agric.Sci.,2008(3):34-35.
[23]朱大恒,张闪闪,莫骏坚,等.光强对湘南烟草生育后期光合及生理特性的影响[J].江苏农业科学,2018,46(8):81-84.Zhu D H,Zhang S S,Mo J J,et al..Effects of light intensity on photosynthesis and physiological characteristics of tobacco in late growth period in southern Hunan[J].Jiangsu Agric.Sci.,2018,46(8):81-84.
[24]谢兆文,王寿昆,林旋,等.高温胁迫对仿刺参抗氧化酶等指标的影响[J].水产科学,2016,35(4):327-333.Xie Z W,Wang S K,Lin X,et al..Effects of high temperature stress on antioxidant enzymes and other indexes of apostichopus japonicus[J].Fisheries Sci.,2016,35(4):327-333.
[25]任彩虹,闫桂琴,郜刚,等.高温胁迫对马铃薯幼苗叶片生理效应的影响[J].中国马铃薯,2007,21(1):5-10.Ren C H,Yan G Q,Gao G,et al..Effects of high temperature stress on physiological effects of potato seedling leaves[J].China Potato J.,2007,21(1):5-10.
[26]杜秀敏,殷文璇,张慧,等.超氧化物歧化酶(SOD)研究进展[J].中国生物工程杂志,2003,23(1):48-50.Du X M,Yin W X,Zhang H,et al..Research progress of superoxide dismutase(SOD)[J].J.China Biotechnol.,2003,23(1):48-50.
[27]朱雪梅,柯永培,邵继荣,等.高温胁迫对重穗型水稻品种叶片活性氧代谢的影响[J].种子,2005,24(3):25-27.Zhu X M,Ke Y P,Shao J R,et al..Effects of high temperature stress on activated oxygen metabolism of the leaves of the heavy panicle type of medium India hybrid rice[J].Seed,2005,24(3):25-27.
[28]孙胜楠.设施黄瓜对高温胁迫的响应与适应[D].山东泰安:山东农业大学,硕士学位论文,2017.Sun S N.Response and adaptation of protected cucumber to high temperature stress[D].Shandong Taian:Shandong Agriculture University,Master Dissertation,2017.
[29]Lee B R,Kim K Y,Jung W J,et al..Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover(Trifolium repens L.)[J].J.Exp.Bot.,2007,58(6):1271.
[30]Sofo A,Dichio B,Xiloyannis C,et al..Antioxidant defences in olive trees during drought stress:Changes in activity of some antioxidant enzymes[J].Funct.Plant Biol.,2005,32(1):351-364.
[31]齐玉鑫,李文滨,任海祥,等.施肥量和种植密度对大豆牡丰7号保护性酶活性的影响[J].黑龙江农业科学,2012(5):47-49.Qi Y X,Li W B,Ren H X,et al..Effects of fertilizer application and planting density on the activity of protective enzyme of soybean Mufeng No.7[J].Heilongjiang Agric.Sci.,2012(5):47-49.
[32]陈顺辉,李文卿,江荣风,等.施氮量对烤烟产量和品质的影响[J].中国烟草学报,2003,9(Z1):36-40.Chen S H,Li W Q,Jiang R F,et al..Effects of nitrogen application on yield and quality of flue-cured tobacco[J].China Tobacco Sci.,2003,9(Z1):36-40.
[33]闫玉秋,方智勇.试论烟草中烟碱含量及其调节因素[J].烟草科技,1996,50(6):31-34.Yan Y Q,Fang Z Y.Try to talk about nicotine content in tobacco and its control factors[J].Tobacco Sci.Technol.,1996,50(6):31-34.
[34]陈延玲,吴秋平,陈晓超,等.不同耐密性玉米品种的根系生长及其对种植密度的响应[J].植物营养与肥料学报,2012,18(1):52-59.Chen Y L,Wu Q P,Chen X C,et al..Different tightness of corn resistant varieties of root growth and its response to planting density[J].J.Plant Nutr.Fertilizer,2012,18(1):52-59.
[35]宋日,刘利,吴春胜,等.根系生长空间对玉米生长和养分吸收的影响[J].西北农林科技大学学报,2009,37(6):58-64.Song R,Liu L,Wu C S,et al..The effect of root growth space on maize growth and nutrient absorption[J].J.Northwest A.F.U.,2009,37(6):58-64.
[36]蒋士东,周冀衡,李强,等.不同处理对烟叶经济性状及化学协调性的影响[J].中国农业科技导报,2017,19(11):100-107.Jiang S D,Zhou J H,Li Q,et al..Effects of different treatments on economic characters and chemical coordination of flue cured tobacco[J].J.Agric.Sci.Technol.,2017,19(11):100-107.
[37]汪丽,刘雷,杨文钰,等.种植密度与施钾量对烤烟品质的影响[J].华北农学报,2007,22(增):106-110.Wang L,Liu L,Yang W Y,et al..Effects of plant population and K application level on the quality of leaves of flue-cured tobacco[J].Acta Agric.Boreali-Sin.,2007,22(Suppl.):106-110.
[38]王程栋,王树声,刘新民,等.曲靖烟区土壤化学性状及海拔对烟叶钾含量的影响[J].中国烟草科学,2013,34(4):25-29.Wang C D,Wang S S,Liu X M,et al..Effects of soil chemical properties and altitude on K content in flue-cured tobacco leaves in Qujing area[J].China Tobacco Sci.,2013,34(4):25-29.
[39]吴家昶,李军营,杨宇虹,等.种植密度对津巴布韦引进品种KRK26烟叶产量质量和化学成分的影响[J].西南农业学报,2011,24(1):38-42.Wu J X,Li J Y,Yang Y H,et al..Effect of different planting density on tobacco leaf yield,quality and chemical components for flue-cured tobacco variety KRK26 introduced from Zimbabwe[J].Southwest China J.Agric.Sci.,2011,24(1):38-42.